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Nutritional Requirements for Mycelial Growth of <i>Ophiocordyceps sinensis</i>on Agar Media

Advances in Microbiology 2018年第11期8卷 821-829页

作者： poyu huang Shoji Ohga Department of Agro-Environmental Sciences Faculty of Agriculture Kyushu University Fukuoka Japan

Ophiocordyceps sinensis has been used as one of the most valuable traditional Chinese Medication. This fungus parasitizes larva of Hepialus armoricanus, and converts each larva into a sclerotium form, in which the fruit body grows. Due to the geographical limitation, where O. sinensis can only be found in Himalayas region, the natural resources are limited and very expensive. This research aims to compare the growth-rate of O. sinensis mycelia with different ingredients mix with agar media using one-factor-at-a-time method. This research demonstrated the mycelial growth-rate with different carbon sources, including monosaccharide (Fructose, Glucose), disaccharide (Maltose, Sucrose), and polysaccharide (Dextrin, Malt extract), complex organic nitrogen sources, including beef extract, yeast extract, whey protein, and soy protein, and eight different carbon to nitrogen ratios. The objective of this research is to find out the suitable carbon and organic complex nitrogen sources and ratio for the O. sinensis solid cultivation. As results, O. sinensis grew best with disaccharides comparing to the other types of carbon sources. Furthermore, O. sinensis preferred whey protein in contrast to other organic complex nitrogen sources. As for the carbon to nitrogen ratios, an optimal ratio of 18:1 was observed. Based on those experiments, carbon source shows a greater influence for the mycelial growth. Hence many different types of grains and cereals would be great candidates as the main ingredients for the O. sinensis solid cultivation.

关键词： Ophiocordyceps sinensis Solid Cultivation Disaccharides Whey Protein C/N Ratios Grains

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Utilization of Ceramic Beads for Edible Mushrooms Cultivation

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Advances in Microbiology 2017年第12期7卷 853-862页

作者： poyu huang Shoji Ohga Department of Agro-environmental Sciences Faculty of Agriculture Kyushu University Fukuoka Japan

This study examined ceramic beads as a reusable material for cultivation of edible mushrooms. There are 20 species of popular edible mushrooms in Japan all of which were tested. Within the cultivation vessels, 70% were ceramic beads (diameter 1 cm) and 30% of the nutrient solution. Moreover, the control groups used several types of sawdust, wheat bran, and rice bran with the ratio of 8:1:1 as the substrate. Two sets of substrates were evaluated with the fruit bodies yield. The result indicated that there were 11 species that responded well with the ceramic bead substrate when compared to the traditional sawdust substrate with Agrocybe cylindrica and Pleurotus ostreatus performed the best adding 70 g more of the fruit bodies. Conversely, nine species responded poorly with the ceramic beads substrate with Auricularia polytricha performed the worst losing 120 g. Ceramic beads as a reusable material for substrates not only provide a clean and controllable environment for mycelium to colonize but also deliver more aeration and water availability inside the cultivation vessels. The application of the ceramic bead cultivation can be viewed as an alternative solution for producing Ophiocordyceps sinensis on the industrial level.

关键词： Ceramic Bead Cultivation Edible Mushroom Ophiocordyceps sinensis Aeration Availability Water Potential

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Cultivation of <i>Flammulina velutipes</i>on Modified Substrate Using Fermented Apple Pomace

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Advances in Microbiology 2017年第11期7卷 719-728页

作者： Chikao Hiramori Katsuki Koh Sayaka Kurata Yutaka Ueno Sanath Gamage poyu huang Shoji Ohga Kyozen Syoji Co. Ltd. Nagano Japan Interdisciplinary Graduate School of Science and Technology Shinshu University Nagano Japan Faculty of Agriculture Shinshu University Nagano Japan Graduate School of Agriculture Shinshu University Nagano Japan Faculty of Agriculture Kyushu University Fukuoka Japan

In present study, a cultivation experiment was performed on modified substrates for the Flammulina velutipes, which contains 0% (control group), 4.8% (S group), 9.6% (M group) and 14.4% (L group) of fermented apple pomace (FAP) substituting corncob on a dry matter basis. The pH of all substrates was maintained at the required level, although the pH of FAP was low (3.9). Addition of FAP affected the mycelial growth and full colonizing in test groups to a minor degree, but extended the period by one day in L group. The initiations of fruit bodies were extended in all FAP groups by one day, but there was a little difference in terms of total cultivation time duration among groups due to FAP groups showing raise of growth rates in later phase. The effect of FAP on fruit bodies yield was observed clearly: the yield has the positive correlation with increasing levels of FAP. Fruit bodies showed little difference concerning Brix degree, proximate composition, and organic acid profile among groups. In conclusion, it is suggested that FAP can be used as an alternative to corncobs as a F. velutipes substrates raw material.

关键词： Flammulina velutipes Mushroom Cultivation Fermented Apple Pomace

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基于Arcgis的风区分布图绘制方法研究

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科技通报 2018年第12期34卷 219-222+235页

作者：黄欢犹珀玉彭赤曾华荣罗洪杜昊贵州电网有限责任公司电力科学研究院贵州电力设计研究院

将贵州省视为主要研究区域对象,其中贵州省包含84个气象站观测点,其周边省份包含9个气象站观测点。对贵州省气象站观测点所观测的近30年(1981～2014年)的风速数据和气压数据进行处理。其中风速数据和气压数据分别为离地10m高度10min内... 详细信息

将贵州省视为主要研究区域对象,其中贵州省包含84个气象站观测点,其周边省份包含9个气象站观测点。对贵州省气象站观测点所观测的近30年(1981～2014年)的风速数据和气压数据进行处理。其中风速数据和气压数据分别为离地10m高度10min内平均最大风速数据以及DEM数据,采用克里金内插方法、协同克里金内插方法及数学建模法绘制贵州30年一遇最大风速分区图。通过对比3种绘制方法的结果表明:在空间分布上看,克里金与协同克里金插值法能够得到效果较好的图形分区,图形分区的细节变化可依据数学建模法得到较好的体现;对数据误差进行分析得出,克里金与协同克里金值法能够绘制出最小数据误差的风区图。

关键词：风区图绘制克里金插值法协同克里金插值法数学建模

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黄平县古梯田景观格局与坡改梯策略分析

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贵州师范大学学报（自然科学版） 2015年第5期33卷 15-23页

作者：詹奉丽兰安军付宇文钟九生刘发勇犹珀玉贵州师范大学地理与环境科学学院贵州贵阳550001 贵州省水土保持监测站贵州贵阳550002 贵州师范大学中国南方喀斯特研究院贵州贵阳550001

古梯田的形成与发展充分证实了坡改梯工程是山区水土保持的一项重要措施.通过无人机遥感技术获得分辨率为0.1～0.5m的遥感影像,利用ArcGIS等地理信息系统软件进行数据处理,结合景观软件Fragstats3.3计算特征指标,对贵州省黄平县4个古梯... 详细信息

古梯田的形成与发展充分证实了坡改梯工程是山区水土保持的一项重要措施.通过无人机遥感技术获得分辨率为0.1～0.5m的遥感影像,利用ArcGIS等地理信息系统软件进行数据处理,结合景观软件Fragstats3.3计算特征指标,对贵州省黄平县4个古梯田景观格局进行研究.结果显示：研究区梯田景观和林地景观约占整个景观的80％,其中梯田约占30％,林地约占50％;单块田块平均面积多集中在100～200m2、高度0.7 ～1.7m、宽度0.5 ～0.6m;所在坡度为5～25°的梯田最多,约占总田块数的70％.运用古梯田中蕴含的水土保持思想,为坡改梯工程以及坡耕地水土流失治理提供借鉴.

关键词：古梯田景观格局坡改梯黄平县

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许芝银治疗良性甲状腺结节经验

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中医药临床杂志 2019年第7期31卷 1264-1266页

作者：黄家辉马朝群南京中医药大学江苏南京210000 南京中医药大学附属医院江苏南京210000

介绍许芝银教授运用理气化痰破瘀法治疗良性甲状腺结节的经验。现临床上良性甲状腺结节的患者逐渐增多,许芝银教授总结本病的病机主要为气机郁滞、痰瘀互结,治疗以理气化痰破瘀为主,遣方用药时灵活运用现代药理学研究成果,多有良效,附验... 详细信息

介绍许芝银教授运用理气化痰破瘀法治疗良性甲状腺结节的经验。现临床上良性甲状腺结节的患者逐渐增多,许芝银教授总结本病的病机主要为气机郁滞、痰瘀互结,治疗以理气化痰破瘀为主,遣方用药时灵活运用现代药理学研究成果,多有良效,附验案1则,以资佐证。

关键词：良性甲状腺结节理气化痰破瘀许芝银名医经验

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