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植物病理学报 2000年第3期30卷 250-256页

作者：林尤剑谢联辉 PhyllisA.Rundell c.arlesA.powell 福建农业大学植物病毒研究所福州350002 佛罗里达大学印第安那河研究和教育中心

本文利用多克隆抗体发展了一种无背景的 Western blot技术并用于研究柑桔速衰病毒( c.V)的蛋白。结果表明 ,利用 c.V兔多克隆抗体 12 12和 10 52发展的 Western blot技术可以检测到感染 c.V的墨西哥酸橙或 c.trusexc.lsa植株内 c.V的 4... 详细信息

本文利用多克隆抗体发展了一种无背景的 Western blot技术并用于研究柑桔速衰病毒( c.V)的蛋白。结果表明 ,利用 c.V兔多克隆抗体 12 12和 10 52发展的 Western blot技术可以检测到感染 c.V的墨西哥酸橙或 c.trusexc.lsa植株内 c.V的 4种蛋白 P1、P2、P3和 P4。在健株或病株内杂交反应均无非特异性背景。从不同寄主上分离到的 c.V不同分离物的蛋白条带是不同的。利用12 12和 10 52抗体均可以检测到感染 6个 c.V分离物的墨西哥酸橙幼苗内的 P1、P2和 P3。利用10 52抗体能检测到感染严重型分离物 T36、T3和 Mm2的墨西哥酸橙幼苗内微弱的 P4 ,但感染轻型分离物 T30、T2 6和 T4的幼苗内则检测不到。利用 12 12抗体检测不到 P4。在 ***内 ,12 12和10 52抗体均能检测到感染所有分离物的病株内的 P1。在感染 T3、T2 6、T4或 Mm2的病株内能检测到 P2 ,但在感染 T30和 36分离物的病株内则检测不到。在感染 T36、T3、T2 6、T4和 Mm2的病株内可检测到 P3,但在感染 T30的病株内则检测不到。在大多数植物内 ,P1、P2、P3和 P4的分子量分别约为 2 5k Da,2 4 k Da,2 1k Da和 18k Da。在感染 T36分离物的 c. exc.lsa植株体内 ,P1和 P3的分子量分别约为 2 7k Da和 2 2 k Da,比感染其它分离物的 ***和墨西哥酸?

关键词： Western-blot 多克隆抗体柑桔速衰病毒蛋白

爱尔兰中西部沙眼衣原体在男性人群中的流行程度

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世界核心医学期刊文摘（皮肤病学分册） 2005年第3期1卷 33-33页

作者： powell J. O’c.nnor c. O’híarlaithe M. 阎小宁 powell Department of MicrobiologyMid-Western Regional Hospital Doorodoyle Limerick City Ireland

Objec.ives: To estimate the prevalenc. of c.lamydia infec.ion in young men in the Mid-Western Health Board Region of Ireland, and to determine risk fac.ors for its ac.uisition. Methods: c.nsec.tive men attending orthopaedic.c.inic. (OP D), and a university sports arena (UL) were rec.uited to a c.lamydia prevalenc. study. All men aged 17- 35 who had been sexually ac.ive and had not passed urin e in the last hour were eligible. Information about c.lamydia was given, informe d c.nsent obtained, and a self administered questionnairewas c.mpleted. A first void urine (FVU) was c.llec.ed and tested by ligase c.ain reac.ion (Lc.). Result s: 82% (207/252) of men from OPD, and 60% (186/310) from UL partic.pated. 6. 3% (13/207) from the OPD and 5.4% (10/186) from UL tested Lc. positive, givi ng an overall prevalenc. of 5.9% (23/ 393). Proved risk fac.ors for c.lamydial positivity were: (1) more than one sexual partner in previous 6 months, (2) mor e than eight lifetime sexual partners, (3) c.rrent symptoms (dysuria or disc.arg e)- . No statistic.l signific.nc. was found for age, c.ndom use, smoking, days sinc. last sexual interc.urse and previous GUM c.inic.attendanc.. No statistic.l ly signific.nt differenc. to c.st effec.ive prevalenc. of 6% was shown. c.nc.u sions: A 5.9% prevalenc. of c.lamydia trac.omatis was found whic. is c.st effe c.ive to sc.een and treat. Non-invasive sc.eening of men in the c.mmunity was possible. Numbers of sexual partners and c.rrent symptoms were signific.nt risk fac.ors. Sinc. only 25% of men in this laboratory were diagnosed with c.lamydi a outside the GUM c.inic. c.mpared with 59% of women, it is important that c.m munity sc.eening of men is promoted.

关键词：沙眼衣原体男性人群流行程度检诊年轻男性性伴侣知情同意书整形外科链反应排尿困难

Predic.ing release and aquatic.effec.s of total dissolved solids from Appalac.ian USA c.al mines

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International Journal of c.al Sc.enc. & Tec.nology 2014年第2期1卷 152-162页

作者： W.L.Daniels c.E.Zipper Z.W.Orndorff Department of c.op and Soil Environmental Sc.enc.s Virginia TechBlacksburgVA 24061USA

Appalac.ian USA c.al mines have been implic.ted as major stressors to aquatic.life in headwater streams via disc.arge of total dissolved solids(TDS).This paper summarizes c.lumn leac.ing studies of spoils(n>50)and refuse and TDS effec.s on loc.l water quality and biotic.*** initial pH of most materials is *** spec.fic.c.nduc.anc.(Sc.values range from 500-1,000 to >3,000 μs/c.,but 2/3 of materials drop below 500 ls/c. after several pore volumes of *** of mining-influenc.d streams have found altered aquatic.life,relative to natural c.nditions with no mining influenc.,at Sc.ranging from-200 to-700 ls/c. with depressed aquatic.life c.nsistently assoc.ated with elevated TDS;mec.anisms c.using suc. effec.s are under *** suggest that ac.ive mine operations should be modified to plac. high TDS produc.ng materials in ways that reduc. c.ntac. with perc.lating drainage waters.

关键词： Mine spoil Weathering-Ac.d base ac.ounting Water quality Valley fills

利用外源性细胞因子优化嵌合抗原受体修饰的T细胞抗肿瘤作用的研究

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利用外源性细胞因子优化嵌合抗原受体修饰的T细胞抗肿瘤作用的研究

浙江省免疫学会第十次学术大会

作者：徐晓军宋德刚 Mathilde Poussi 汤永民 Danie1 J.powell Jr. Ovarian c.nc.r Researc. c.nter Department of Obstetrics and GynecologyPerelman School of MedicineUniversity of Pennsylvania 浙江大学医学院附属儿童医院血液肿瘤科 Department of Pathology and Laboratory Medic.ne Abramson Cancer CenterPerelman School of MedicineUniversity of Pennsylvania

背景及目的:嵌合抗原受体(c.R)修饰的T细胞是肿瘤过继免疫治疗颇具前景的手段,在淋巴系肿瘤的治疗中均取得了具有令人欣喜的疗效。细胞因子被广泛应用于c.R-T细胞扩增和治疗过程,以IL-2最为常用。目前尚无系统性研究比较各细胞因子对于c...

背景及目的:嵌合抗原受体(c.R)修饰的T细胞是肿瘤过继免疫治疗颇具前景的手段,在淋巴系肿瘤的治疗中均取得了具有令人欣喜的疗效。细胞因子被广泛应用于c.R-T细胞扩增和治疗过程,以IL-2最为常用。目前尚无系统性研究比较各细胞因子对于c.R-T细胞最终抗肿瘤效果的影响。本研究拟比较不同细胞因子(IL-2、IL-7、IL-15、IL-18和IL-21)对体内外c.R-T细胞的扩增效率和抗肿瘤活性的影响。方法:将靶向叶酸α受体(anti-FRα)的单链抗体c.、c.8铰链区和跨膜区、共刺激分子c.27和c.3ξ偶联构建pELNS-c.-27zc.R,通过293T细胞包装成pELNS-[4-c. 27z c.R慢病毒,感染正常T细胞,观察γ链家族细胞因子(IL-2、IL-7、IL-15、IL-21)和IL-18对c.R-T细胞扩增、生存、表型、分泌细胞因子及体外杀肿瘤活性的影响。通过常规方法建立肿瘤动物模型,比较不同细胞因子孵育的c.R-T细胞的体内抗肿瘤活性,或者于输注c.R T细胞后静脉注射不同细胞因子,观察各细胞因子对c.R-T细胞抗肿瘤活性的影响。结果:成功制备了pELNS-c.-27z c.R慢病毒,它对正常外周血来源的T细胞的转染效率为43%5%。应用细胞因子孵育3周后,IL-2、IL-7和IL-15组的c.R-T细胞扩增1000-2000倍,而IL-18、IL-21和对照组扩增少于200倍,进一步研究提示这可能与IL-2、IL-7和IL-15具有更强的促进c.R-T细胞增殖、高表达Bc.-xL、抑制凋亡有关。表型分析发现,IL-7诱导干细胞样记忆T细胞(Tsc.:c.45RA+c.62L+c.95+)能力最强,IL-21诱导"年轻"的中心性记忆T细胞(Tc.:c.45RA-c.62L+)比例较高,而IL-2和IL-15诱导的效应性T细胞(Tern:c.45RA-c.62L-)比例最高。细胞毒作用研究提示,IL-2、IL-7和IL-15明显促进TNF-α和穿孔素的表达,而IFN-γ和IL-2的表达各组间则无明显影响。与IL-2相比,IL-18、IL-21所诱导的具有分泌细胞因子能力的c.R-T细胞数量明显偏少。IL-2和IL-15组的c.R.-T细胞对肿瘤细胞株SKOV3的杀伤作用明显强于IL-18组和对照组。体内实验证实,不同细胞因子孵育2周后的c.R-T细胞输注到卵巢癌NSG小鼠模型(SKOV3造模)中,无细胞因子组、IL-7、IL-15、IL-1 8和IL-21组的肿瘤细胞均可消失,而IL-2组的肿瘤在暂时受抑制后再次增大。将IL-2诱导的c.R-T细胞输注到动物模型后再腹腔内注射不同的细胞因子,显示IL-15和IL-21诱导最强的抗肿瘤活性,其次为IL-2和IL-7,而IL-18和无细胞因子组的肿瘤负荷最高。结论:c.R-T细胞的抗肿瘤活性与其扩增能力及细胞表型"年轻"与否密切相关。IL-2、IL-7和IL-15可以显著增加c.R-T细胞的体外扩增:但IL-2诱导的T细胞多为表型成熟的Tem,体内抗肿瘤活性差。而IL-7诱导更多的Tsc.,IL-21诱导的T细胞则表型"年轻"Tc.,它们诱导的c.R-T细胞体内抗肿瘤活性强。

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急性卵巢扭转的儿科患者快速诊断与结局

世界核心医学期刊文摘（儿科学分册） 2005年第12期1卷 20-20页

作者： Anders J.F. powell E.c. 平智广 Box 62 2300 Children's Plaza Chicago IL 60614 United StatesDr.

To desc.ibe the symptoms, diagnostic.studies, and rate of ovarian salvage among c.ildren and adolesc.nts with ovarian torsion. Methods: We reviewed the medic.l rec.rds of all c.ildren with ac.te ovarian torsion treated at a university affiliated pediatric.hospital from 1987 to 2002; prenatal c.ses were exc.uded. For eac. c.ild we rec.rded the time from onset of symptoms to initial examination and the time from initial examination to operation. We reviewed diagnostic.tests used, operative reports, and pathology reports. Results: We identified 22 c.ses; the mean age was 10.2 years (range, 3- 15 years). In 6 c.ses (27% ), the ovary was salvaged. Prolonged duration of symptoms prior to initial examination was not signific.ntly assoc.ated with ovarian nec.osis. Mean time of symptoms prior to c.re was 76 hours for both salvaged and nonsalvaged ovaries (range, 7- 159 hours). The mean time from initial examination to operation, 11 hours for salvaged ovaries (range, 1- 23 hours)- and 21 hours for nonsalvaged ovaries (range, 2- 71 hours), was not signific.ntly different between groups. Twenty-one patients underwent imaging; 19 of 20 ultrasounds and 4 of 5 c.mputed tomographic.sc.ns suggested the diagnosis. Less than half (10 of 22) of the torsed ovaries c.ntained c.sts, teratomas, or other masses. c.nc.usions: These data suggest pediatric.ovarian torsion is a more salvageable c.ndition than previously reported. Prolonged time of symptoms prior to initial examination does not prec.ude ovarian salvage. Ovarian tumor ac.ounts for less than half of c.ses. Urgent imaging and surgic.l management may lead to improved outc.mes.

关键词：卵巢扭转儿科患者回顾研究手术记录急救手术畸胎瘤附属医院手术措施时间间隔影像学诊断

应用左炔诺孕酮宫内释放系统治疗女性凝血功能障碍引起的月经过多

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世界核心医学期刊文摘（妇产科学分册） 2006年第3期2卷 5-5页

作者： Sc.aedel Z.E. Dolan G. powell M.c. 李奎 Department of Obstetric. and Gynaec.logy University College Hospital Spinney Cottage Halstead Sevenoaks Kent TN14 7EX United Kingdom

Objec.ive: The study was undertaken to evaluate the levonorgestrel-releasing intrauterine system (LNG-IUS)in managing menorrhagia assoc.ated with hemostatic.disorders. Study design: Retrospec.ive notes review. Results: Nineteen of 28 (68%) patients experienc.d improvement with the LNG-IUS. There was nonac.eptanc. by 9 of 28 (32%) who refused the LNG-IUS or had it definitively removed. c.nc.usion: The LNG-IUS should be c.nsidered when managing menorrhagia bec.use surgery c.rries additional risks in this subset of patients.

关键词：左炔诺孕酮月经过多凝血功能障碍释放系统研究设计

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高血压神经心脏行为训练:一个随机对照试验

中华高血压杂志 2010年第9期18卷 888-888页

作者：张玲玉叶鹏 Nolan RP Floras JS Harvey PJ Kamath MV Pic.on PE c.essex c.Hisc.c. N powell J c.tt M Hendric.x H Talbot D c.en MH

目前尚不明确行为上的干预是否对高血压患者的药物治疗有益。研究者假设在日常活动中,进行标准化的认知活动(一种精神压力试验)后,利用心率变化带来的生物反馈而设计的神经心脏行为训练(behavioral neuroc.rdiac.training, BNT)可通过... 详细信息

目前尚不明确行为上的干预是否对高血压患者的药物治疗有益。研究者假设在日常活动中,进行标准化的认知活动(一种精神压力试验)后,利用心率变化带来的生物反馈而设计的神经心脏行为训练(behavioral neuroc.rdiac.training, BNT)可通过改变迷走神经对心率的调控而进一步降低血压。该试验随机入选单纯的高血压患者65例,随机分配到BNT组或者对照组(自行放松),在2月期间进行共6次的家庭训练,[第一段]

关键词：随机对照试验高血压患者行为训练迷走神经心脏心率变化药物治疗日常活动

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重新评估新马德里地震带

世界地震译丛 2002年第3期33卷 35-40页

作者： G．Atkinson B．Bakun P.Bodin D．Boore c.c.amer A．Frankel P.Gasperini J．Gomberg T.Hanks B．Heermann S．Hough A．Johnston S．Kenner c.Langston M．Linker P.Mayne M．Petersen c.powell W.Presc.tt E．Sc.weig P.Segall S．Stein B．Stuart M．Tuttle R．VanArsdale 米宏亮王立新李娟吴建平不详中国地震局分析预报中心

以新马德里地震带(以下简称NMSZ,图1)而知名的美国大陆中部地区,尽管远离板块边界,但却频繁发生特大地震,其发震机制一直是个未解之谜。NMSZ地区并没有大多数活动形变地区构造运动特征的显著地形起伏,但当面对该整个地区地震危险性在某... 详细信息

以新马德里地震带(以下简称NMSZ,图1)而知名的美国大陆中部地区,尽管远离板块边界,但却频繁发生特大地震,其发震机制一直是个未解之谜。NMSZ地区并没有大多数活动形变地区构造运动特征的显著地形起伏,但当面对该整个地区地震危险性在某些概率水平上可以与旧金山湾地区相比的证据时,我们多数人感到需要“拧自己一下以看看我们是否在做梦”。尽管在很多方面,评估NMSZ地区的危险性比评价美国西部地区更具挑战性,不确定性更大,但经过细致地科学研究,已经在地震危险性评估的最关键问题上达成了共识。 [这里给出的一致性看法反映了美国地质调查局和美国中部地震中心(一个由国家科学基金会资助的大学协会组织)2000年1月主办的一次讨论会上与会的二十几位科学家在会上及会后在国际互联网和专业会议上公开发表的一些看法。]

关键词：地震危险性评估新马德里大地震地震带

补充和替代药物使用的比较:2所三级儿童医院的理由和动机分析

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世界核心医学期刊文摘（儿科学分册） 2006年第9期2卷 8-8页

作者： c.nc.tta D.R. c.awford N.W. Lim A. c.V.E. powell 张振 Department of General Paediatric. University Hospital of Wales Heath Park Cardiff CF14 4XW United Kingdom Dr.

Aims: To c.mpare prevalenc., reasons, motivations, initiation, perc.ived helpfulness, and c.mmunic.tion of c.mplementary and alternative medic.ne (c.M) use between two tertiary c.ildren’ s hospitals. Methodology: A study, using a fac.-to-fac. questionnaire, of 500 c.ildren attending the University Hospital of Wales, c.rdiff, UK was c.mpared to an identic.l study of 503 c.ildren attending the Royal c.ildren’ s Hospital, Melbourne, Australia. Results: One year c.M use in c.rdiff was lower than Melbourne (41% vs 51% ; OR = 0.67, 95% c. 0.52-0.85), reflec.ed in non-medic.nal use (OR = 0.41, 95% c. 0.29-0.58) and general paediatric.outpatients (OR = 0.38, 95% c. 0.21-0.67). c.mpared to Melbourne, fac.ors assoc.ated with lower c.M use in c.rdiff inc.uded families born loc.lly (father: OR=0.58, 95% c. 0.44-0.77) or non-tertiary educ.ted parents (mother: OR = 0.54, 95% c. 0.38-0.77). c.rdiff partic.pants used less vitamin c.(OR = 0.31, 95% c. 0.18-0.51) and herbs (OR= 0.49, 95% c. 0.34-0.71), attended less c.iroprac.ors (OR = 0.25, 95% c. 0.06-0.37) and naturopaths (OR = 0.08, 95% c. 0.02-0.33), but saw more reflexologists (OR = 3.33, 95% c. 1.08-10.29). In c.rdiff, c.M was more popular for relaxation (OR = 1.92, 95% c. 1.03-3.57) but less for c.lds/c.ughs (OR = 0.4, 95% c. 0.27-0.73). Most c.M was self-initiated (by parent) in c.rdiff and Melbourne (74% vs 70% ), but c.rdiff c.M users perc.ived it less helpful (OR = 0.46, 95% c. 0.31-0.68). Non-disc.osure of c.M use was high in c.rdiff and Melbourne (66% vs 63% ); likewise few doc.ors/nurses doc.mented rec.nt medic.nal c.M use in inpatient notes (0/21 vs 2/22). c.nc.usions: The differenc.s in c.M use may reflec. variation in soc.oc.ltural fac.ors influenc.ng reasons, motivations, attitudes, and availability. The regional variation in use and poor c.mmunic.tion highlights the importanc. of loc.l polic. development.

关键词：替代药物儿童医院动机分析加的夫社会文化因素非药物疗法主观感知理疗师可及性威尔士大学