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In vitro cytotoxic and toxicological activities of ethanolic extract of Kaempferia galanga Linn. and its active component,ethyl-p-methoxycinnamate, against cholangiocarcinoma

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Journal of Integrative Medicine 2020年第4期18卷 326-333页

作者： porwornwisit tritripmongkol Tullayakorn Plengsuriyakarn Mayuri Tarasuk Kesara Na-Bangchang Graduate Program in Bioclinical Sciences Chulabhorn International College of MedicineThammasat UniversityPathum Thani 12120Thailand Center of Excellence in Molecular Biology and Pharmacology of Malaria and Cholangiocarcinoma Chulabhorn International College of MedicineThammasat UniversityPathum Thani 12120Thailand Drug Discovery and Development Center Office of Advanced Science and TechnologyThammasat UniversityPathum Thani 12120Thailand

Objective:To evaluate the cytotoxic,apoptotic,mutagenic and immunomodulatory activities of Kaempferia galanga Linn.(KG)extract and ethyl-p-methoxycinnamate(EPMC)in ***:The present study investigated the cytotoxic[using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphe nyl-2-H-tetrazolium bromide test],apoptotic(using a mitochondrial membrane potential assay),mutagenic(using a micronucleus test)and immunomodulatory(using flow cytometry)activities of the ethanolic extract of KG and its bioactive component,EPMC,against two cholangiocarcinoma(CCA)cell lines,CL-6 and HuCCT1,and one normal human cell line,OUMS-36 T-1 ***:Both KG extract and EPMC exhibited moderate cytotoxic activity against both CCA *** cytotoxic activity was supported by their concentration-dependent induction of ***-6 was most sensitive(3–4 fold)and selective to 5-fluorouracil(5-FU),compared with KG extract and EPMC[median half inhibiting concentration(IC50)and selectivity index(SI)were 23.01 lg/mL and 17.32;78.41 lg/mL and 4.44;100.76 lg/mL and 2.20,respectively for 5-FU *** extract ***].HuCCT1 was relatively more sensitive and selective to 5-FU and EPMC than KG extract[median IC50 and SI were 66.03 lg/mL and6.04;60.90 lg/mL and 3.65;156.60 lg/mL and 2.23,respectively for 5-FU *** *** extract].EPMC produced relatively potent cytotoxic activity against polymorphonuclear cells(IC50=92.20 lg/mL).KG extract and EPMC exhibited concentration-dependent mutagenic activity,as well as inhibition of tumor necrosis factor-a and ***:Considering cytotoxic,apoptotic,immunomodulatory and mutagenic activities,further development of KG as a drug candidate is likely to focus on the oral pharmaceutical formulation of a standardized KG extract rather than isolated compounds.

关键词： Kaempferia galanga Linn. Cytotoxicity Cholangiocarcinoma Mutagenicity Apoptosis Immunomodulatory activity

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