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基因工程抗体片段——谱写抗体家族的新乐章

中国肿瘤生物治疗杂志 2011年第5期18卷 467-472页

作者：崔恒高磊北京大学人民医院妇科肿瘤中心北京100044

随着分子生物学技术的进步和抗体基因结构的阐明,以小分子抗体为基础并经基因工程改建从而用于研究和临床诊治的大量基因工程抗体片段应运而生。其中,可将生物学活性效应桥连于治疗靶标的双特异性抗体、同时增强抗体效能及细胞毒靶向性... 详细信息

随着分子生物学技术的进步和抗体基因结构的阐明,以小分子抗体为基础并经基因工程改建从而用于研究和临床诊治的大量基因工程抗体片段应运而生。其中,可将生物学活性效应桥连于治疗靶标的双特异性抗体、同时增强抗体效能及细胞毒靶向性的免疫结合物、以独特的单域结构在生物技术和临床医学等方面倍受青睐的纳米抗体,以及在细胞内特定部位表达的细胞内抗体是近年来研究最活跃的几个领域。凭借免疫原性弱、组织渗透性强、低毒性及制备简便等优势,基因工程抗体片段较之单克隆抗体受到了更广泛的关注与认可。尽管大多数处于临床开发的抗体片段的安全性和有效性还有待确定,但基因工程抗体片段的研发必将大大提高人类疾病尤其是肿瘤的诊治水平。

关键词：抗体片段基因工程双特异性抗体免疫结合物纳米抗体细胞内抗体抗肿瘤作用

X-射线吸收谱原位表征cu-Zn/Si._2催化剂cu价态

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化学学报 2018年第8期76卷 639-643页

作者：贾臻龙涂云宝王建强 Frenkel Anatoly i. 杨为民刘仲能许中强中国石油化工股份有限公司上海石油化工研究院上海201208 Yeshi.a Uni.ersi.y Physics Department

铜基催化剂的价态组成与制备方法、还原温度有关,也是决定其催化性能的关键因素.本文对比研究cu-Zn/Si._2和cu/Si._2催化剂前驱体在不同还原温度下的价态组成及其醋酸甲酯加氢性能.采用同步辐射X射线吸收谱原位表征催化剂前驱体的cu K边... 详细信息

铜基催化剂的价态组成与制备方法、还原温度有关,也是决定其催化性能的关键因素.本文对比研究cu-Zn/Si._2和cu/Si._2催化剂前驱体在不同还原温度下的价态组成及其醋酸甲酯加氢性能.采用同步辐射X射线吸收谱原位表征催化剂前驱体的cu K边的X射线近边吸收谱(XANES),通过线性组合分析方法(LCF)拟合XANES吸收谱得到了催化剂前驱体在不同还原温度下氧化态(cu^(2+)、cu^+)和金属态(cu0)的组分含量.结果表明:(1)采用蒸氨法添加Zn制备的cu-Zn/Si._2催化剂前驱体铜组分还原度高;(2)催化剂前驱体在还原过程中cu^+主要存在于低温还原阶段(cu^0+cu^+含量较低(≤40%);(3)金属态铜是醋酸酯加氢反应的活性中心.

关键词： cu/Si.2 cu-Zn/Si.2 醋酸甲酯乙醇 XANES

Twi.ni.g i. i.termetalli. Compounds Are Long Shear Vectors and/or Shuffles Really Necessary?

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Journal of Materi.ls Sci.nce & Technology 1993年第5期9卷 313-321页

作者： F.M.Chu Davi. P.pope Dept.of Materi.ls Sci.nce and Engi.eeri.g,Uni.ersi.y of Pennsylvani.,Phi.adelphi.,PA 19104,USA

i. thi. paper the geometri. descri.ti.n and general theory of mechani.al twi.ni.g are revi.wed, the twi.s i. general latti.es and superlatti.es are summari.ed, and the ki.eti. process by whi.h mechani.al twi.s form i. revi.i.ed. A case study of mechani.al twi.ni.g of HfV2+Nb, (cubi.) Laves phase, i. presented and the synchroshear of selected atomi. layers i. proposed to explai. the physi.al process of twi. formati.n. i. the twi.s form i. thi. way, then long shear vectors and / or atomi.shuffles are not really necessary.

关键词： twi. i.termetalli. compounds shear vector shuffles

Thermomechani.al Analysi. of (cu_(0.5)Tl_(0.5))-1223 Substi.uted by Pr and La

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Journal of Materi.ls Sci.nce & Technology 2012年第2期28卷 169-176页

作者： A.i.Abou Aly R.Awad i.H.i.rahi. M.Kamal M.Anas Physi.s Department Faculty of ScienceAlexandria University Physi.s Department Faculty of ScienceMansoura University

The thermomechani.al analysi. （TMA） of cu0.5T10.5Ba2Ca2-xRxcu30110-δ, where R=Pr and La, wi.h 0.0〈x〈0.15, was carri.d out i. temperature range from 450 to 1145 K. The samples were prepared by si.glestep soli. state reacti.n techni.ue. The prepared samples were characteri.ed by X-ray di.fracti.n （XRD） and scanni.g electron mi.roscopy （SEM）. The superconducti.i.y of the prepared samples was i.vesti.ated by electri.al resi.ti.i.y measurement. The results showed that low substi.uti.n content enhanced the （cu0.5Tlo.5）- 1223 phase formati.n, whi.e the hi.her substi.uti.n content degraded thi. phase. The hi.her superconducti.g transi.i.n temperatures Tc were found to be 114 K and 109 K at x= 0.025 for Pr- and La-substi.uti.ns, respecti.ely. The average li.ear thermal expansi.n coeffi.i.nt i.creased as x i.creased, whi.e the shri.kage temperature decreased as x i.creased. Those results were emphasi.ed by porosi.y and Vi.kers mi.rohardness calculati.ns. Debye temperature 0D was calculated from the li.ear thermal expansi.n coeffi.i.nt data and correlated to Tc to esti.ate the electron-phonon coupli.g λep.

关键词： (cu0.5Tl0.5)-1223 Thermomechani.al analysi. Thermal expansi.n coeffi.i.nt Debye temperature

Spati.l Di.tri.uti.n of Heavy Metals i. Agri.0}ltural Soi.s of an i.dustry-Based Peri.Urban Area i. Wuxi. Chi.a

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Pedosphere 2007年第1期17卷 44-51页

作者： ZHAO Yan-Feng SHi.Xue-Zheng HUANG Bi.o YU Dong-Sheng WANG Hong-Ji. SUN Wei.Xi. i. OEBOERN K. BLOMBACK State Key Laboratory of Soi. and Sustai.able Agri.0}lture Institute of Soil Science Chinese Academy of Sciences Nanjing 210008 （China） Department of Soi. Sci.nce the Swedish University of Agricultural Sciences SE-75007 Uppsala （Sweden） Graduate School of the Chi.ese Academy of Sci.nces Beijing 100039 （China）

i. i.dustry-ori.nted peri.urban areas, the heavy metal accumulati.n i. soi.s caused by i.dustri.li.ati.n has become a potenti.l threat. The top soi. samples from 27 paddy fi.lds and 75 vegetable fi.lds were collected from a typi.al i.dustry- based peri.urban area of about 8 km^2 i. Wuxi. Chi.a, to study the accumulati.n and di.tri.uti.n of As, Hg, cu, Zn, Pb, Cr, and Cd i. compari.on wi.h heavy metal contents i. soi.s near developed i.dustri.l si.es （Guangzhou, Chi.a; Wallsend Burn of Tynesi.e, UK; and Osnabrück, Germany）. Kri.i.g i.terpolati.n was used to determi.e the metals, spati.l di.tri.uti.n. The results showed that most soi.s, compared to the background values, contai.ed elevated contents of As, Hg, cu, Zn, and Pb wi.h some havi.g elevated contents of Cd and Cr. Except for less than 10% of the soi. samples of cu, Zn and Cd contents, these heavy metal contents were lower than the soi. threshold levels of the Grade Ⅱ cri.eri. for the Chi.ese envi.onmental quali.y standard. Probably, because of the scattered di.tri.uti.n and di.ersi.y of i.dustri.s i. the study area, spati.l di.tri.uti.ns of these heavy metals from Kri.i.g i.terpolati.n i.di.ated li.tle si.i.ari.y. Nevertheless, when compared wi.h other areas i. the Tai.u Lake regi.n, mean contents of cu, Zn, Pb, and Cd were relati.ely hi.h i. the Wuxi.peri.urban area. Addi.i.nally, compared to soi.s i. agri.0}ltural areas around Guangzhou, Osnabrück, or Wallsend Burn, contents of most heavy metals i. soi.s from thi. area were lower.

关键词： agri.0}ltural soi. heavy metals peri.urban area spati.l di.tri.uti.n

Synthesi. and Characteri.ati.n of GLBCO-123 Phase: Gd1-xLxBa2cu3O7-δ(x = 0.0 - 0.5)

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Journal of Materi.ls Sci.nce and Chemi.al Engi.eeri.g 2017年第11期5卷 49-57页

作者： Made Sumadi.asa i. Gusti.Agung Putra Adnyana Nyoman Wendri.Putu Suardana Department of Physi.s Faculty of Mathematics and Natural Sciences Udayana University Badung Indonesia

i.vesti.ati.n of the substi.uti.n process can provi.e a better understandi.g of the superconducti.g mechani.ms i. cuprous oxi.e materi.ls. i. thi. work the effects of substi.uti.n Lanthanum (La) for Gadoli.i.m (Gd) on the structure and oxygen content for x = 0.0 - 0.5 i. the compound Gd1-xLaxBa2cu3O7-δ (GLBCO-123 phase) have been i.vesti.ated. Samples were synthesi.ed by usi.g a wet-mi.i.g method from powders of Gd2O2, La2O2, BaO, cuO, and soluti.n of HNO3. Based on the analysi. of XRD data and SEM-EDXA, i. confi.ms that the sample has formed the GLBCO-123 phase, as expected. i. has been obtai.ed that the latti.e parameters a and c are i.creased whi.e the parameter b i. sli.htly decreased wi.h i.creasi.g content of Lanthanum. The oxygen content sli.htly decreased and structure of the Gd1-xLaxBa2cu3O7-δ phase changed from orthorhombi. to tetragonal wi.h i.creasi.g the content of Lanthanum.

关键词： Gd1-xLaxBa2cu3O7-δ Phase La Substi.uti.n Latti.e Parameters Orthorhombi. Oxygen Content

人成纤维细胞样细胞株产生的肿瘤细胞生长抑制因子的初步提取物及其性质

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中国医学科学院学报 1987年第1期9卷 7-12页

作者：吴克复 Sculley TB pope JH Ellem KAO 中国医学科学院血液学研究所澳大利亚昆士兰医学研究所(Qi.R) 澳大利亚昆士兰医学研究所(Qi.R) 天津

用Sephadex G-200,DEAE-Bi.-Gel A和Blue-agarose层析取得人成纤维细胞产生的肿瘤细胞生长抑制因子(HFDi.的初步提取物。用Sephadex G-200和高效液相层折(HPLC)测定,估算其分子量约为55000。HFDi.提物可耐受56℃,半h,于pH 4～9、4℃下2... 详细信息

用Sephadex G-200,DEAE-Bi.-Gel A和Blue-agarose层析取得人成纤维细胞产生的肿瘤细胞生长抑制因子(HFDi.的初步提取物。用Sephadex G-200和高效液相层折(HPLC)测定,估算其分子量约为55000。HFDi.提物可耐受56℃,半h,于pH 4～9、4℃下24 h,并可在-70℃保存数周。初提物的肿瘤细胞敏感谱与细胞洗液相似,均未查出干扰素活性。

关键词：肿瘤抑制因子成纤维细胞 Burki.t 淋巴瘤黑色素瘤

Preli.i.ary Study towards Enhanced Crude Oi. Bi.degradati.n Reveals Congeneri. Total Peroxi.ases wi.h Stri.i.g Di.ti.cti.ns

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Advances i. Enzyme Research 2015年第3期3卷 66-74页

作者： Folasade M. Olajuyi.be Kevi. i. Ehi.sun Ki.elomo F. Jai.esi.i Enzyme Bi.technology Uni. Department of Biochemistry Federal University of Technology Akure Nigeria

Peroxi.ases (POXs) are the key extracellular enzymes produced by crude oi. degradi.g mi.robes. Knowledge of opti.um condi.i.ns for POXs acti.i.y i. cruci.l for provi.i.g effecti.e envi.onment for bi.remedi.ti.n. i. thi. study, physi.ochemi.al properti.s of POXs produced by Acti.omyces i.raeli. and Acti.omyces vi.cosus duri.g growth on crude oi. were studi.d. The POXs exhi.i.ed si.i.ari.i.s i. acti.i.y and stabi.i.y wi.h stri.i.g di.ferences i. response to two di.alent metal i.ns. The POXs from both speci.s had opti.um pH of 7.0 and were very stable over a narrow pH range (6.0 - 8.0). The POXs demonstrated si.i.ar thermostabi.i.y exhi.i.i.g relati.e resi.ual acti.i.y of 62% at 50°C after 30 mi. i.cubati.n and 45% resi.ual acti.i.y at the same temperature after 60 mi. despi.e the fact that POXs from A. vi.cosus and A. i.raeli. had opti.um temperatures of 50°C and 40°C, respecti.ely. The POXs from A. vi.cosus and A. i.raeli. were greatly acti.ated by Fe2+ at 5.0 and 10.0 mM. The enzymes were both strongly i.hi.i.ed by cu2+, Mg2+ and Hg2+. Surpri.i.gly, these congeneri. POXs demonstrated stri.i.g di.ferences i. thei. response to Ca2+ and Mn2+. POX from A. vi.cosus was acti.ated by Ca2+ and Mn2+ exhi.i.i.g relati.e acti.i.y of 136% and 106% at 5 mM, respecti.ely. i. contrast, POX from A. i.raeli. was strongly i.hi.i.ed by Ca2+ and Mn2+ exhi.i.i.g 62.5% relati.e acti.i.y i. the presence of 5 mM of each metal i.n. i.creasi.g the concentrati.n of Ca2+ and Mn2+ led to further acti.ati.n of POX from A. vi.cosus and i.hi.i.i.n of POX from A. i.raeli.. Results provi.e deeper i.si.hts i.to functi.nal properti.s of studi.d POXs from closely related mi.robes. The physi.ochemi.al properti.s are very si.i.ar;however, notable di.ferences provi.e a strong basi. for structural characteri.ati.n of these congeneri. enzymes.

关键词： Acti.omyces i.raeli. Acti.omyces vi.cosus Bi.remedi.ti.n Congeneri. Enzymes Crude Oi. Peroxi.ases

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竖直旋喷桩在风积沙隧道洞口段的应用研究

现代隧道技术 2019年第S2期56卷 712-718页

作者：刘罡醋经纬中交第一公路勘察设计研究院有限公司西安710075

某公路改扩建工程隧道穿越厚层风积沙地层,风积沙地层具有结构松散、颗粒间粘聚力极低、抗剪强度低等特点,除洞身段围岩稳定性极差外,明洞亦存在地基承载力不足的问题。为解决隧道洞口段稳定性及地基承载力不足的问题,采用一种一次钻孔... 详细信息

某公路改扩建工程隧道穿越厚层风积沙地层,风积沙地层具有结构松散、颗粒间粘聚力极低、抗剪强度低等特点,除洞身段围岩稳定性极差外,明洞亦存在地基承载力不足的问题。为解决隧道洞口段稳定性及地基承载力不足的问题,采用一种一次钻孔、分段喷浆的旋喷桩对洞口段进行加固处理。采用通用离散元软件UDEC对风积沙隧道洞口段进行力学行为分析,结合现场监控量测结果表明,竖直旋喷桩对风积沙隧道洞口段的稳定性及地基承载力起到了明显的改善作用,可为类似工程提供一定的参考。

关键词：公路隧道竖直旋喷桩数值模拟风积沙洞口段