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Journal of Zhejiang University Science 2002年第3期3卷 348-354页

作者： Sateeshk.mar 徐孟奎陈玉银 ponnuvel,k.m Datta,R.k DepartmentofSericulture&Apiculture ZhejiangUniversityHangzhou310029.China molecularBiologyLaboratory CentralSerlculturalResearchandTrainingInstituteMysore570008India

Two silkworm strains viz, B20 A (high cocoon shell ratio) and *** (low cocoon shell ratio) were sib mated for 10 generations to determine the homozygosis. Both bulked segregant analysis(BSA) and near isogenic lines (NIL) studies were done to identify the RFLP markers closely linked to cocoon shell parameters. Three hundred and fifty two random clones were identified as the low copy number sequence and used for identification of Restriction Fragment Length Polymorphic (RFLP) marker linked to cocoon weight and cocoon shell character. In the bulk segregant analysis, DNA from the parents (B20 A, ***), F 1 and F 2 progeny of high shell ratio (HSR) and low shell ratio (LSR) were screened for hybridization with the random clones. Polymorphic banding pattern achieved through southern hybridization with different probes indicated the probable correlation of polymorphism with high and low cocoon shell character which are possible landmarks in identifying the putative marker(s) for the cocoon shell character. Out of the 100 probes tried with parents, F 1, F 2 and their bulks, 10 probes were found to be closely linked to cocoon shell characters.

关键词： Restriction fragment length polymorphic (RFLP) molecular marker Bombyx mori L Shell ratio Bulked segregant analysis(BSA) Near isogenic lines

Regression analysis of squeezing-induced hybrid nanofluid flow in Darcy-Forchheimer porous medium

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Applied mathematics and mechanics(English Edition) 2025年第1期46卷 193-208页

作者： k.mUHAmmAD m.SARFRAZ Department of Humanities and Sciences School of Electrical Engineering and Computer Science National University of Sciences and Technology Department of mathematics Quaid-i-Azam University

This article presents a mathematical model addressing a scenario involving a hybrid nanofluid flow between two infinite parallel plates. One plate remains stationary, while the other moves downward at a squeezing velocity. The space between these plates contains a Darcy-Forchheimer porous medium. A mixture of water-based fluid with gold(Au) and silicon dioxide(Si O2) nanoparticles is formulated. In contrast to the conventional Fourier's heat flux equation, this study employs the Cattaneo-Christov heat flux equation. A uniform magnetic field is applied perpendicular to the flow direction, invoking magnetohydrodynamic(mHD) effects. Further, the model accounts for Joule heating, which is the heat generated when an electric current passes through the fluid. The problem is solved via NDSolve in mATHEmATICA. Numerical and statistical analyses are conducted to provide insights into the behavior of the nanomaterials between the parallel plates with respect to the flow, energy transport, and skin *** findings of this study have potential applications in enhancing cooling systems and optimizing thermal management strategies. It is observed that the squeezing motion generates additional pressure gradients within the fluid, which enhances the flow rate but reduces the frictional drag. Consequently, the fluid is pushed more vigorously between the plates, increasing the flow velocity. As the fluid experiences higher flow rates due to the increased squeezing effect, it spends less time in the region between the plates. The thermal relaxation, however, abruptly changes the temperature, leading to a decrease in the temperature fluctuations.

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Species diversity of birds in mangroves of kundapura, Udupi District, karnataka, Southwest Coast of India

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Journal of Forestry Research 2014年第3期25卷 661-666页

作者： Vijaya k.mar k.m Vijaya k.mara Department of Zoology Bhandarkars’ Arts and Science College Department of Post Graduate Studies and Research in Wildlife and management Bioscience Block Kuvempu University

We quantified species diversity of birds in mangroves at kundapura from April-2010 to march-2013. We recorded 79 species of 36 families and 14 orders. Of these 71% are resident species, 22% are residential migrants and 8% are migratory. One endangered species, three near threatened species, and a few occasional visitors were re-corded. Species diversity and abundance of birds were greater during from October through may as there was availability of food, increased vegetation and the arrival of migratory birds. minimum diversity was recorded from June through September owing to heavy rains, increased flow of water, limited availability of food and return of migratory birds.

关键词： Species, mangroves, birds, threatened migratory

Sublethal Effects of the Formamidine Amitraz on Honeybees Gut Lipids, Following in vivo Injections

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Biomedical and Environmental Sciences 1991年第4期4卷 376-383页

作者： k.m'DIAYE m.BOUNIAS Laboratory of Biochemistry INRA-Avignon B.P.91 F 84143 Montfavet Cédex FranceLaboratory of Biochemistry INRA-Avignon B.P.91 F 84143 Montfavet Cédex France

The time-course and dose-related action of amitraz (AmZ) on gut lipids of worker honeybees were examined over 3 hours following in vivo injections of 0.25, 0.5, 1 and 2 nmols of pesticide per bee. Significant decreases were observed at 30-45 min with 0.25 nmols per bee, for phospholipids, fatty acids, steroids and triacylglycerols. However increases were observed either later or with higher doses. The decreasing action observed with 0.25 nmol AmZ per bee was inhibited by simultaneous injections of the a antagonist phentolamine (from 0.25 to 2.0 nmols per bee). The toxicity of AmZ to honeybees thus likely involves the mobilization of lipids from the gut, via action of this formamidine pesticide on a-adrenoceptors.

关键词： Sublethal Effects of the Formamidine Amitraz on Honeybees Gut Lipids Following in vivo Injections

One Pot Synthesis of medicinally Important ***-4-am i no s u bstituted-1,2,3,4-tetrahyd roq u i nolines

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Chinese Journal of Chemistry 2012年第3期30卷 534-540页

作者： kiran,k.mar H.C mahadevan,k.m Prabhakara,VarmaP Srinivasa,A Department of Post Graduate Studies and Research in Chemistry School of Chemical SciencesKuvempu UnivesityShankaraghatta-577451KarnatakaIndia Bioorganics andApplied materials Pvt.Ltd. PIABangalore-58KarnatakaIndia Cdymax(India)Pharma Pvt.Ltd.116/117 KIADB Industrial AreaJiganiBangalore-560105KarnatakaIndia

A simple synthesis of medicinally important cis-2-methyl-4-azapan-2-one-1,2,3,4-tetrahydroquinolines/cis-9- （2-methyl-l,2,3,4-tetrahydroquinolin-4-yl）-9H-carbazole was reported. multicomponent one pot synthesis with ani- lines and N-vinylcaprolactam/N-vinyl carbazole via imino Diels-Alder reaction by using antimony trichloride as catalyst and acetonitrile as solvent was employed. NmR technique （2D） was used to study the regio- and stereo- chemistry of newly synthesized compounds. The cis diastereo-selectivity of the products was predicted by COSY and NOESY studies.

关键词： imino Diels-Alder antimony trichloride tetrahydroquinoline N-vinylcaprolactam N-vinylcarbazole

molecular characterization of DnaJ 5 homologs in silkworm Bombyx mori and its expression during egg diapause

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Insect Science 2014年第6期21卷 677-686页

作者： Sasibhushan Sirigineedi Esvaran Vijayagowri Geetha N. murthy Guruprasada Rao kangayam m. ponnuvel Genomics Division Seribiotech Research Laboratory Carmelaram Post Kodathi Bangalore 560035 Karnataka India

A comparison of the cDNA sequences （1 056 bp） of Bombyx mori DnaJ 5 homolog with B. mori genome revealed that unlike in other Hsps, it has an intron of 234 bp. The DnaJ 5 homolog contains 351 amino acids, of which 70 contain the conserved DnaJ domain at the N-terminal end. This homolog orB. mori has all desirable functional domains similar to other insects, and the 13 different DnaJ homologs identified in B. mori genome were distributed on different chromosomes. The expressed sequence tag database analysis of Hsp40 gene expression revealed higher expression in wing disc followed by diapause-induced eggs. microarray analysis revealed higher expression of DnaJ 5 homolog at 18th h after oviposition in diapause-induced eggs. Further validation of DnaJ 5 expression through qPCR in diapause-induced and nondiapause eggs at different time intervals revealed higher expression in diapause eggs at 18 and 24 h after oviposition, which coincided with the expression of Hsp70 as the Hsp 40 is its co-chaperone. This study thus provides an outline of the genome organization of lisp40 gene, and its role in egg diapause induction in B. mori.

关键词： Bombyx mori diapause DnaJ exon Hsp40 intron

Time and Dose-related Effects of the Pyrethroid Fluvalinate on Haemolymph Carbohydrates and Gut Lipids of Honeybees,Following in vivo Injection of very Low Doses

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Biomedical and Environmental Sciences 1993年第2期6卷 145-153页

作者： k.m'DIAYE m.BOUNIAS University University of AvignonBiomathematics & Toxicology23 rue du 58eR.I.F 84000 AvignonFranceof AvignonBiomathematics & Toxicology23 rue du 58eR.I.F 84000 AvignonFrance

The injection to emerging adult workerbees with fluvalinate doses ranging from 1 femtomol to 1 nanomol per individual resulted in a reduction of haemolymph carbohydrate concentrations, particularly at the lowest dose 1 hour after injections. At the same time, a large increase was observed for triacylglycerols and to a much lesser extent for steroids and phospholipids with 0.1 picomol per bee. By contrast, fatty acids, steroids and triacylglycerols exhibited a depress at the higher dose. most responses were thus biphasic, showing that much attention should be paid to the effects of very low doses of pesticide.

关键词： Time and Dose-related Effects of the Pyrethroid Fluvalinate on Haemolymph Carbohydrates and Gut Lipids of Honeybees,Following in vivo Injection of very Low Doses very

Online tuning of PID controllers using delayed state variable filters

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Online tuning of PID controllers using delayed state variabl...

Analysis of genetic relationship in mutant silkworm strains of Bom-byx mori using inter simple sequence repeat (ISSR) markers

计算机，通信，控制与电力工程国际会议

作者： Tsang,k.m Rad,A.B To,F.W. Hong kong Polytechnic Department of Electrical Engineering

ISBN: (纸本)780003285X

Delayed state variable filters are implemented for the estimation of monotone open-loop processes which can be approximated by a dominant time delay coupled with a first order *** on the delayed filtered step output response,the first and second order derivatives,the corresponding static process gain,the apparent dead time and the apparent time constant are evaluated.A new PID tuning formula is formed which based on the placement of the open loop zeros related to the apparent time constant and time *** with the root locus design procedure,the algorithm provides a range of PID settings for different damping *** are included to illustrate the concepts.

关键词： origin Online tuning of PID controllers using delayed state variable filters

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Journal of Genetics and Genomics 2008年第5期35卷 291-297页

作者： Dhanikachalam Velu kangayam m. ponnuvel murugiah muthulakshmi Randhir k. Sinha Syed m.H. Qadri Biotechnology Laboratory Central Sericultural Germplasm Resources Centre Hosur-635109 Tamil Nadu India

Amplified inter simple sequence repeats （ISSR） markers were used to determine genetic relationships among mutant silkworm strains of Bombyx mori. Fifteen ISSR primers containing simple sequence repeat （SSR） motifs were used in this study. A total of 113 markers were produced among 20 mutant strains, of which 73.45% were found to be polymorphic. In selected mutant genetic stocks, the average number of observed allele was （1.7080 ± 0.4567）, effective alleles （1.5194 ± 0.3950） and genetic diversity （Ht）（0.2901 ± 0.0415）. The dendrogram produced using the unweighted pair group method with arithmetic means （UPGmA） and cluster analysis made using Nei＇s genetic distance resulted in the formation of one major group containing 6 groups separated 20 mutant silkworm strains. Therefore, ISSR amplification is a valuable method for determining the genetic variability among mutant silkworm strains. This efficient molecular marker would be useful for characterizing a considerable number of silkworm strains maintained at the germplasm center.

关键词： genetic diversity Bombyx mori mutant strains ISSR

The sexually dimorphic expression of glutamate transporters and their implication in pain after spinal cord injury

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Neural Regeneration Research 2025年第11期20卷 3317-3329页

作者： Jennifer m.Colón-mercado Aranza I.Torrado-Tapias Iris k.Salgado Jose m.Santiago Samuel E.Ocasio Rivera Dina P.Bracho-Rincon Luis H.Pagan Rivera Jorge D.miranda Department of Physiology University of Puerto RicoMedical Sciences CampusSan JuanPRUSA National Institutes of Health National Human Genome Research InstituteBethesdaMDUSA Universidad Central del Caribe School of MedicineBayamónPRUSA University of Puerto Rico Carolina CampusCarolinaPRUSA Institute of Neurobiology San JuanPRUSA

In addition to the loss of motor function,~60% of patients develop pain after spinal cord *** cellular-molecular mechanisms are not well understood,but the data suggests that plasticity within the rostral,epicenter,and caudal penumbra of the injury site initiates a cellularmolecular interplay that acts as a rewiring mechanism leading to central neuropathic *** can lead to the formation of new connections triggering abnormal sensory *** excitatory glutamate transporters are responsible for the reuptake of extracellular glutamate which makes them a critical target to prevent neuronal hyperexcitability and *** previous studies showed a sexually dimorphic therapeutic window for spinal cord injury after treatment with the selective estrogen receptor modulator *** this study,we investigated the anti-allodynic effects of tamoxifen in male and female rats with spinal cord *** hypothesized that tamoxifen exerts anti-allodynic effects by increasing the expression of glutamate transporters,leading to reduced hyperexcitability of the secondary neuron or by decreasing aberrant *** and female rats received a moderate contusion to the thoracic spinal cord followed by subcutaneous slow-release treatment of tamoxifen or matrix pellets as a control(placebo).We used von Frey monofilaments and the“up-down method”to evaluate mechanical *** treatment decreased allodynia only in female rats with spinal cord injury revealing a sexdependent *** expression profile of glutamatergic transporters(excitatory amino acid transporter 1/glutamate aspartate transporter and excitatory amino acid transporter 2/glutamate transporter-1)revealed a sexual dimorphism in the rostral,epicenter,and caudal areas of the spinal cord with a pattern of expression primarily on *** rodents showed a significantly higher level of excitatory amino acid transporter-1 expression while male rodents showed increased

关键词： allodynia central neuropathic pain EAAT-1/GLAST EAAT-2/GLT-1 glutamate transporters selective estrogen receptor modulator sexual dimorphism spinal cord injury trauma