检索结果-南通市图书馆

Virulence Factors in Methicillin-Resistant staphylococcus aureus Isolated from ICU Units in Brazil

Advances in Microbiology 2014年第4期4卷 207-215页

作者： simone G.souza Guilherme B.Campos pollianna s.oliveira Daniel s.sousa Danilo C.C.Da silva Verena M.santos Aline T.Amorim Angelita M.O.G.santos Jorge Timenetsky Mariluze P.Cruz Regiane Yatsuda Lucas M.Marques Instituto Multidisciplinar em saúde Núcleo de Tecnologia em SaúdeUniversidade Federal da BahiaVitória da ConquistaBrazil Instituto de Ciências Biomédicas Departamento de MicrobiologiaUniversidade de Sao PauloSao PauloBrazil

species of staphylococcus are common in hospital infection (HI). Methicillin resistant s. aureus (MRsA) has also become a serious problem in Brazilian HI. The aim of this study was to characterize the pathogenicity of methicillin-resistant s. aureus (MRsA) and methicillin-sensitive staphylococcus aureus (MssA) isolated in public hospitals. The clinical isolates were obtained from intensive care unit. The MRsA and MssA strains were genotyped by PCR for detection genes related to virulence factors. Moreover, the strains were tested for biofilm formation and cytokine induction in macrophages. Three strains of MRsA (9.68%) expressed the sea gene, one (3.23%) seb, 17 (54.84%) spa and seven (22.58%) had PVL. Two MssA strains (2.98%) expressed the sea gene, three (4.48%) seb, 18 (26.87%) spa and 11 (16.42%) showed positive results for the PVL gene. There was no expression of sec and CflA between MRsA and MssA strains. Among MRsA and MssA isolates, none statistical differences were observed in biofilm production. The analysis of cytokine induction in the inflammatory response of J774 macrophages by MRsA and MssA isolates did not show statistical difference. Understanding the mechanisms of pathogenesis of s. aureus could provide important clues for both preventing and treating infection caused by these organisms.

关键词： MRsA MssA Virulence Factor Hospitalar Infection

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Virulence Factor Profile of staphylococcus aureus Isolated from Bovine Milk from Brazil

引用

Food and Nutrition sciences 2014年第15期5卷 1496-1505页

作者： Verena M.santos Hellen B.Martins Izadora s.Rezende Maysa s.Barbosa Ewerton F.Andrade simone G.souza Guilherme B.Campos pollianna s.oliveira Daniel s.sousa Danilo C.C.Da silva Aline T.Amorim Jorge Timenetsky Mariluze P.Cruz Regiane Yatsuda Lucas M.Marques Instituto Multidisciplinar em saúde Núcleo de Tecnologia em SaúdeUniversidade Federal da BahiaVitoria da ConquistaBrazil Instituto de Ciências Biomédicas Departamento de MicrobiologiaUniversidade de Sao PauloSao PauloBrazil

This study investigates the biofilm formation, presence and distribution of virulence genes and the capacity to induce an inflammatory response in strains of staphylococcus aureus isolated from milk samples in Bahia, Brazil. A total of 132 samples of raw milk were collected from four dairy farms (designated A to D) located in southwestern Bahia, in the municipality of Vitória da Conquista, from October/2009 to september/2010. After processing of the samples, 94 (71.2%) s. aureus isolates were obtained. These strains were subjected to the antibiogram method MIC (Minimum Inhibitory Concentration). As for the pathogenicity, tests were performedin vitrobiofilm formation induced by glucose. Moreover, we performed PCR for their virulence genes: sea (enterotoxin A), seb (B), sec (C), pvl (Panton-Valentine Leukocidin), clfA (Clumping Factor A) and spa (protein A) and analysis of cytokine induction in the inflammatory response of J774 macrophages by exocellular lipoteichoic acid. No isolates were resistant to oxacillin and vancomycin. In biofilm production, 5.31% (5/94) isolates did not produce biofilm, 5.31% (5/94) of the samples were poor producers, 15.96% (15/94) strains were moderate producers, 18.09% (17/94) were producers and 55.32% (55/94) of isolates were strong biofilm producers. One (1.06%) isolate expressed the seb gene, one (1.06%) sec, 18 (19.2%) cflA and 44 (46.8%) had spa. There was no expression of sea and pvl between isolates analyzed. The analysis of cytokine induction in the inflammatory response did not show statistical difference in the levels of IL-6, TNF-α and IL-10 induction. However, there was statistical difference in IL-1 induction between isolates from different farms. Thus, it appears that the results obtained in this study show significant effects for the region studied, since it is an important dairy region, hence the need for further studies, with the intent of attracting funding that contributes to improving prevention and control in both dai

关键词： Milk staphylococcus aureus Resistance Virulence Factors

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Efficiency in Teaching speaker and Listener Repertoires:Comparing Three Instructional sequences in Autistic Children

引用

Psychology Research 2023年第6期13卷 235-245页

作者： Daniel Carvalho de Matos pollianna Galvão soares de Matos Neylla Cristina Pereira Cordeiro Katiane Reis da silva Universidade Ceuma Brasil Universidade Federal do Maranhão and Instituto EvoluirSão Luís cityBrazil Universidade Ceuma and Instituto Evoluir São Luís cityBrazil

Previous studies have investigated the efficiency in teaching listener and speaker repertoires in children diagnosed with autism spectrum disorder(AsD).some investigations focused on listener responding by function,feature,and class(LRFFC)and intraverbal by function,feature,and class(FFC).For some children,teaching intraverbal FFC was more efficient because it resulted in a better emergence effect of a related untaught repertoire(LRFFC).For other children,teaching LRFFC along with tacting pictures was more efficient,resulting in a better emergence effect of a related untaught repertoire(intraverbal FFC).In these cases,it is not clear whether the tact increased the efficiency of LRFFC training because a comparison with a condition in which tacts were not required was not *** investigation consisted of a replication with two children diagnosed with *** instructional sequences were compared:teaching LRFFC-probing intraverbal;teaching LRFFC+tacts-probing intraverbal;teaching intraverbal-probing *** a child,all sequences were equally efficient because all related untaught repertoires emerged without ***,the acquisition of intraverbals during training occurred with *** the case of the second child,the most efficient sequence consisted of teaching intraverbals,resulting in the emergence of LRFFC without *** both cases of teaching LRFFC,the emergence of related intraverbals was partial and acquisition of the trained repertoires occurred with *** case that did not demand tact responses was slightly more *** were discussed in the sense that the best instructional sequence may vary from learner to learner.

关键词： autism spectrum disorder listener repertoire speaker repertoire efficiency

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Performance of the school Psychologist in schools for the Inclusion of Autistic students in the scenario of COVID-19

引用

Psychology Research 2023年第2期13卷 60-74页

作者： Vinicius Cutrim Ferreira pollianna Galvão soares de Matos Daniel Carvalho de Matos Francidalma soares sousa Carvalho Filha Universidade Ceuma São LuísBrazil Instituto Evoluir São LuísBrazil Universidade Federal do Maranhão São LuísBrazil Universidade Estadual do Maranhão São LuísBrazil

During COVID-19,the repercussions on scholar dynamics,functioning and structure were inevitable,causing an impact on groups that,historically,are minority in relation to the scope of school inclusion policies,such as students diagnosed with autism spectrum disorder(AsD).This scenario is followed by educational phenomena in the face of which school Psychology has collaborated with the advancement of research and knowledge *** qualitative study,based on Historical-Cultural Psychology and Critical school Psychology,analyzed the role of the school psychologist in monitoring students diagnosed with AsD in the context of regular school during the *** following procedures were conducted:application of an online questionnaire;and semi-structured interviews with four school *** a result,three zones of shared meanings were built:the claim for the right to education with support and adaptations;the psychologist as the great savior of inclusion;the non-total inclusion that denounces the structure of injustice by *** information suggests that professional performance was hampered by different obstacles in the pandemic,such as the difficulty imposed by school,overload of demands,and impasses in the implementation of inclusion historically experienced at school,revealing that the school structure still carries historical consequences of *** shared meanings have demanded a professional performance with more effort,resistance,and strategies that accept the demands of school inclusion,providing collective mobilization,together with school actors,capable of breaking down barriers,especially those related to capacitist concepts.

关键词： school psychology autism spectrum disorder school inclusion pandemic

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：