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Cladogynos orientalis Zipp. extracts inhibit cell culture-derived hepatitis C virus genotype 2a replication in Huh-7 cells through NS5B inhibition

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Asian Pacific Journal of Tropical Biomedicine 2019年第8期9卷 346-352页

作者： piyanoot thongsri Khanit Sa-ngiamsuntorn Pongtip Sithisarn Mullika T. Chomnawang Krit Thirapanmethee Department of Microbiology Faculty of PharmacyMahidol UniversityBangkok 10400Thailand Department of Biochemistry Faculty of PharmacyMahidol UniversityBangkok 10400Thailand Department of Pharmacognosy Faculty of PharmacyMahidol UniversityBangkok 10400Thailand

Objective: To evaluate the potential anti-hepatitis C virus (HCV) activities of Cladogynos orientalis Zipp. ex Span and to investigate the molecular mode of action. Methods: Ethanolic and water extracts from various parts of Cladogynos orientalis were examined for cytotoxicity by MTT assay. Sub-cytotoxic concentrations of the extracts were used for further determining anti-HCV activity using cell culture-derived HCV genotype 2a propagated in HepaRG cell line. Immunofluorescence assay was performed to observe the effect on viruses at the pre-entry step. Mode of action at the post-entry step was investigated for the viral RNA and protein expressions by real time RT-PCR and Western blotting assays, respectively. Results: Although Cladogynos orientalis water extracts exhibited lower cytotoxicity than ethanolic extracts, all ethanolic extracts from roots, stems, and leaves of Cladogynos orientalis exhibited higher anti-HCV activities than water extracts. The highest anti-HCV activity was observed in infected cells treated with the extracts 5 h after absorption. No extracts showed pre-viral entry effect. At the post-viral entry step, only leaf ethanolic extracts inhibited NS5B expression, while all extracts did not inhibit HCV NS3 expression. Conclusions: Cladogynos orientalis ethanolic extracts could be further studied and the major active compound needs to be identified as a promising source for anti-HCV agents.

关键词： Cladogynos orientalis Hepatitis C virus Cell culture-derived HCV genotype 2a

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