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Correlations between Gene Resistant Markers and Second-Line Anti-TB Drug Resistance in Pre-XDR and XDR-TB Patients

Journal of Tuberculosis Research 2017年第3期5卷 178-188页

作者： Risara Jaksuwan Prasit Tharavichikul Charoen Chuchottaworn Jayanton Patumanond piyada kunawararak Jongkolnee Settakorn Clinical Epidemiology Unit Faculty of Medicine Chiang Mai University Chiang Mai Thailand Department of Microbiology Faculty of Medicine Chiang Mai University Chiang Mai Thailand Division of Respiratory Medicine Chest Disease Institute Nonthaburi Thailand Faculty of Medicine Thammasat University Bangkok Thailand Office of Disease Prevention and Control 10 Chiang Mai Thailand Department of Pathology Faculty of Medicine Chiang Mai University Chiang Mai Thailand

Background: Extensively drug resistant tuberculosis (XDR-TB) is a serious problem in public health and XDR-TB patients usually develop from multi-drug resistance tuberculosis (MDR-TB) and pre-XDR-TB. The rapid molecular test for drug susceptibility testing (DST) can be used for early detection to prevent XDR-TB. Methods: We examined 34 clinical Mycobacterium tuberculosis (M. tuberculosis) isolates from MDR/XDR-TB patients in the upper north of Thailand that were identified with drug susceptibility profiles by indirect agar proportion method from 2005-2012. Our study investigated the genetic mutations in gyrA for ofloxacin resistance and rrs for kanamycin resistance. The genetic mutations and drug susceptibility test results were analyzed using the exact test. Results: The majority of the ofloxacin resistance was detected in gyrA 21, gyrA 70, gyrA 87, gyrA 102, gyrA 162, and gyrA 187 were at 0%, 12.5%, 37.5%, 0%, 50.0% and 25.0% sensitivity, respectively, and at 96.2, 96.2%, 20.1%, 96.2%, 57.7% and 61.5% specificity, respectively. Kanamycin resistance was found in rrs 512, rrs 241, rrs 223, rrs 414 and rrs 408 at 16.7%, 0%, 0%, 16.7% and 16.7% sensitivity, respectively, and at 96.4%, 92.9%, 82.1%, 82.1% and 71.4% specificity, respectively. This study found no significant correlation between gyrA mutations and ofloxacin resistance and also no correlation between the rrs gene and kanamycin resistance. Conclusion: These primer sequences and PCR products in our study such as gyrA and rrs might be unsuitable to detect ofloxacin and kanamycin resistance in the upper north of Thailand.

关键词： XDR-TB Pre-XDR-TB Ofloxacin gyrA Kanamycin rrs

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Rice Grains from Slightly Saline Field Exhibited Unchanged Starch Physicochemical Properties but Enhanced Nutritional Values

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Rice science 2024年第3期31卷 343-360,I0055-I0057页

作者： Supranee SANTANOO Wichian SANGWONGCHAI Maysaya THITISAKSAKUL Suphatta PHOTHISET Paweena PONGDONTRI Noppawan NOUNJAN piyada THEERAKULPISUT Department of Biology Faculty of ScienceKhon Kaen UniversityKhon Kaen 40002Thailand Salt-Tolerant Rice Research Group Khon Kaen UniversityKhon Kaen 40002Thailand Biological Science Program Faculty of ScienceKhon Kaen UniversityKhon Kaen 40002Thailand Department of Biochemistry Faculty of ScienceKhon Kaen UniversityKhon Kaen 40002Thailand Department of Food Technology and Nutrition Faculty of Natural Resources and Agro-IndustryKasetsart UniversityChalermphrakiat Sakon Nakhon Province CampusSakon Nakhon 47000Thailand International College Khon Kaen UniversityKhon Kaen 40002Thailand

This study aims to investigate grain quality and nutritional values of rice(Pokkali,a salt-tolerant cultivar;RD73,a new cultivar improved from KDML105 introgressed with Saltol QTL from Pokkali,and KDML105,a moderately salt-susceptible cultivar)grown under non-saline(0.04–0.87 dS/m)and slightly saline(1.08–4.83 dS/m)field *** results revealed that salinity caused significant reduction in grain size but significant increments in reducing sugar and total protein contents in the ***,the amounts of starch in the grains of KDML105 and Pokkali rice genotypes were unaffected by the *** starch granule size distribution was also unaffected by ***,only starch from Pokkali was significantly diminished in amylose content,from 19.18%to 16.99%.Accordingly,parameters relating to starch gelatinization,retrogradation,and pasting properties of KDML105 and RD73 were unaffected by salinity;only Pokkali showed a significant increase in percentage of retrogradation along with a significant reduction in gelatinization *** the saline field,total phenolic content and antioxidant capacity in the grains of all rice cultivars tended to increase,particularly in *** average,essential element contents in grains from the saline-treated plants showed a 33%,32%,32%,22%,20%,11%,and 10%increase in total P,N,K,Mg,Zn,Fe,and Ca content,***,total Fe content exhibited the greatest percentage of increments in KDML105(187%).Taken together,cultivation of rice in the slightly saline field did not alter its eating and cooking qualities,while enhanced some nutritional properties such as proteins,minerals,and secondary metabolites like phenolic compounds.

关键词： antioxidant capacity elemental composition gelatinization grain morphology retrogradation rice salt stress starch granule

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Pollen morphology of the genus Cornukaempferia (Zingiberaceae) in Thailand

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Journal of Systematics and Evolution 2009年第2期47卷 139-143页

作者： Piyaporn SAENSOUK Pranom CHANTARANOTHAI piyada THEERAKULPISUT Applied Taxonomic Research Center Department of BiologyFaculty of ScienceKhon Kaen University

Cornukaempferia is a recently described genus of Zingiberaceae which only occurs in Thailand as a rare genus with limited geographical *** three species have been described so far,including a recently described new species,*** three species are morphologically very similar and additional data on other biological aspects are useful for the elucidation of their *** morphology of all three species of genus Cornukaempferia has been studied by light microscopy and scanning electron *** pollen grains are monad,spherical,*** exine sculpture is echinate with psilate between the spines for *** and ***,or echinate with regulate between the spines for *** obser-vation helps support the taxonomic status of ***.

关键词： Cornukaempferia Zingiberaceae pollen morphology exine

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Development of species-specific SCAR markers for identification of three medicinal species of Phyllanthus

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植物分类学报 2008年第4期46卷 614-621页

作者： piyada THEERAKULPISUT Nantawan KANAWAPEE Duangkamol MAENSIRI Sumontip BUNNAG Pranom CHANTARANOTHAI Applied Taxonomic Research Center Department of Biology Faculty of Science Khon Kaen UniversityKhon Kaen 40002 Thailand

Phyllanthus amarus Schum. & Thonn. has been widely used in traditional medicine in Thailand as an antipyretic, a diuretic, to treat liver diseases and viral infections. Two closely related species, P. debilis L. and P. urinaria Klein ex Willd., with different and less effective medicinal properties, are less commonly used. These three species are similar in morphology and often occur in overlapping populations in nature. The latter two species can easily be mistaken for P. amarus and collected for medicinal uses, which can lead to undesirable results. DNA fingerprints of these species were obtained using RAPD-PCR techniques. RAPD markers specific for each species were identified. Primers for highly specific sequence-characterized-amplified-regions (SCAR) were then designed from nucleotide sequences of specific RAPD markers. These primers efficiently amplified SCAR markers of 408, 501 and 319 bp unique to P. amarus, P. debilis and P. urinaria respectively. This method of plant identification was rapid and highly specific when tested against DNA of several closely related species and was able to amplify specific markers from mixed DNA samples.

关键词：叶下珠中药药物药用价值成份分析

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Differential Proteins Expressed in Rice Leaves and Grains in Response to Salinity and Exogenous Spermidine Treatments

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Rice science 2016年第1期23卷 9-21页

作者： Paweena SALEETHONG Sittiruk ROYTRAKUL Kanlaya KONG-NGERN piyada THEERAKULPISUT Rajamangala University of Technology Isann Surin Campus Surin 32000 Thailand National Center for Genetic Engineering and Biotechnology Pathum Thani 12120 Thailand Salt-tolerant Rice Research Group Department of Biology Faculty of ScienceKhon Kaen University Khon Kaen 40002 Thailand

Exogenous application of spermidine（Spd） has been reported to modulate physiological processes and alleviate salt-induced damage to growth and productivity of several plants including rice. Employing a proteomic approach, we aimed at identifying rice leaf and grain proteins differentially expressing under salt stress, and in response to Spd prior to Na Cl treatment. A total of 9 and 20 differentially expressed protein spots were identified in the leaves of salt-tolerant（Pokkali） and saltsensitive（KDML105） rice cultivars, respectively. Differential proteins common to both cultivars included a photosynthetic light reaction protein（oxygen-evolving complex protein 1）, enzymes of Calvin cycle and glycolysis（fructose-bisphosphate aldolase and triose-phosphate isomerase）, malate dehydrogenase, superoxide dismutase and a hypothetical protein（Os I_18213）. Most proteins were present at higher intensities in Pokkali leaves. The photosynthetic oxygen-evolving enhancer protein 2 was detected only in Pokkali and was up-regulated by salt-stress and further enhanced by Spd treatment. All three spots identified as superoxide dismutase in KDML105 were up-regulated by Na Cl but down-regulated when treated with Spd prior to Na Cl, indicating that Spd acted directly as antioxidants. Important differential stress proteins detected in mature grains of both rice cultivars were late embryogenesis abundant proteins with protective roles and an antioxidant protein, 1-Cys-peroxiredoxin. Higher salt tolerance of Pokkali partly resulted from higher intensities and more responsiveness of the proteins relating to photosynthesis light reactions, energy metabolism, antioxidant enzymes in the leaves, and stress proteins with protective roles in the grains.

关键词： Oryza sativa L proteomics salt stress spermidine

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Groundwater Quality and Arsenic Contamination in Amphoe Khemmarat, Ubon Ratchathani, Thailand

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Journal of Environmental Science and Engineering(A) 2012年第2期1卷 133-141页

作者： Pranee Pattanapipitpaisal t piyada Suraruk BioremediationLaboratory DepartmentofBiologyScienceFacultyofScienceUbonRatchathaniUniversityUbonRatchathani34910Thailand

Arsenic （As） is one of the most important elemental pollutants in groundwater and drinking water because it causes health problem of arsenicosis after consumption of drinking arsenic-rich water more than 5-10 years. Arsenic contamination of groundwater is an emerging issue in Mekong Basin including Cambodia, Vietnam, and Thailand. In Thailand, information about arsenic contamination in drinking water resources are quite rare due to that arsenic is not the main element in water qualification assay. The objective of this study is to determine groundwater quality and arsenic contamination in rural Mekong Basin, Ubon Ratchathani. Groundwater samples were collected from 20 different sampling points, between August 2009 and February 2010 in Amphoe Khemmarat, Ubon Ratchathani, Thailand. Physical and chemical characteristics of groundwater were determined. It was observed that the groundwater was 27.9-30.3 ~C, pH 5.7-6.9. The conductivity was 707-767 ***＂. Dissolved oxygen was 2.04-5.12 mg.L-1 and TDS was 352-384 mg.L~. The samples showed soft- to very hardness-water properties. In some area, few parameters like CI, Fe, Mn, and As exceeded the WHO guideline limits. This result represents basic information for quality of groundwater and the arsenic contamination in rural Mekong Basin, Ubon Ratchathani. Thus, it is probably useful for arsenic standard level assignment and public health authorities. Moreover, it also leads to establish research activity in treatment of arsenic-contaminated groundwater for different purposes

关键词： Arsenic arsenic contamination groundwater quality.

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Identification of Genes for Powdery Mildew Resistance in Mungbean

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Journal of Life Sciences 2010年第5期4卷 25-29页

作者： Parinya Khajudpam Sopone Wongkaew piyada Tantasawat School of Crop Production Technology Suranaree University of Technology Nakhon Ratchasima 30000 Thailand Center for Agricultural Biotechnology （AG-BIO / PERDO-CHE） Bangkok 10330 Thailand

Powdery mildew, which called Sphaerotheca phaseoli in Latin, is one of the major diseases of mungbean （Vigna radiata L.） worldwide, causing up to 50% yield losses. Most mungbean varieties grown in Thailand are susceptible to the disease, therefore, new resistant varieties are highly desirable. Three resistant mungbean lines, V4718, V4758 and V4785, were identified from the AVRDC collection. In this study, the authors compared the resistance levels among these 3 lines and tested the allelic relationship among these resistance genes. Three crosses, V4718 × V4758, V4718 × V4785 and V4758 × V4785, were made and the F1 hybrids were selfed to generate the F2 populations and crossed to a susceptible variety, CN72 to generate the F1 × S populations. In the F1 × S and F2 populations, the resistance segregated in a ratio of 3 Resistant （R）：I Susceptible （S） and 15R：IS, respectively for all three crosses. These results indicate that a single dominant gene confers resistance to powdery mildew in each resistant line and these resistance genes are non-allelic. The authors are currently transferring these resistance genes into commercial varieties to provide durable resistance to powdery mildew.

关键词： Allelism resistance gene Sphaerotheca phaseoli Vigna radiata (L.)

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中国游客赴泰国观看人妖表演的旅游动机研究

中国游客赴泰国观看人妖表演的旅游动机研究

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作者：周雅玲（piyada SRIBUSDI）广西大学

学位级别：硕士

根据泰国旅游局2015年的研究报告显示,观看泰国人妖表演是最受欢迎中国游客的旅游活动之一,尤其是在曼谷、芭提雅以及普吉岛。同时据泰国蒂芬妮人妖表演剧院的调查报告也显示,中国游客的旅游团赴泰国观看人妖表演数最多,比2015年增长16%... 详细信息

根据泰国旅游局2015年的研究报告显示,观看泰国人妖表演是最受欢迎中国游客的旅游活动之一,尤其是在曼谷、芭提雅以及普吉岛。同时据泰国蒂芬妮人妖表演剧院的调查报告也显示,中国游客的旅游团赴泰国观看人妖表演数最多,比2015年增长16%,在自助游的中国游客也发现,观看人妖表演也是他们最主要的旅游活动之一,在报告中还预测在2016年泰国的人妖表演企业将增长30%。因此,研究关于中国游客赴泰国观看人妖表演的旅游动机是创造中泰旅游市场的利润的重要关键。本研究以中国游客赴泰国观看人妖表演的旅游动机、旅游行为及满意度为主要研究目的,通过"Taro Yamane公式”计算样本容量后确定为400人,然后借鉴国内外相关旅游动机的理论和前人研究成果进行调查问卷设计,并通过Cronbach's alpha信度分析法进行问卷中的信度检验,在数据分析方面,本研究主要是采用描述性统计分析法,以分析样本的人口统计学特征、旅游行为特征,包括满意度为主,然后利用因子分析法找出主要的旅游动机,接着通过回归分析进行假设检验。本研究的结果发现,大多数中国游客赴泰国观看人妖表演是中年人,男性居多,并是跟团旅游,至少观看过一次人妖表演,结果也发现,中国游客以便利性与价值的旅游动机为主要的旅游动机;其次为放松与挑战的旅游动机。满意度而言,结果显示大多数中国游客赴泰国观看人妖感到满意,并发现个人的年龄和职业会导致旅游动机和满意度,可是旅游行为不会导致旅游动机,它只会反映游客的满意度。从本研究的结果来看,作者认为泰国地政府、旅游运营商、旅行社,尤其泰国人妖表演剧院需要对人妖表演加强创新力,提高人妖的艺术和汉语水平,并加重职业道德的培训。同时需要不断地提高剧院内的基础设施和技术,包括市场营销,并促进与其他国家扩大合作,这些建议有助于泰国的人妖表演剧院和泰国的人妖表演企业的发展,而且有利于中泰旅游市场的发展。

关键词：中国游客泰国人妖表演旅游动机

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