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人胰岛素基因的合成——Ⅷ.表达型质粒的构建和在大肠杆菌内生产含有胰岛素原的融合蛋白

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生物工程学报 1985年第2期 14-33页

作者：郭礼和 piotr P.stepien 曹仲欣 Roland Brousseau Saran Narang David Y.Thomas 吴瑞中国科学院上海细胞生物学研究所 Division of Biological Sciences National Research Council of Canada Section of Biochemistry Molecular and Cell Biology Cornell University Ithaca NY 14853 U.S.A.

我们已构建两组质粒,适合于在大肠杆菌内克隆基因和直接大量合成融合蛋白。这些质粒含有***的lac启动基因和部分编码β-半乳糖苷酶的序列,编码的蛋白长度约590或450个氨基酸残基(原基因编码1023个氨基酸残基)。被缩短的β-半乳糖苷酶基... 详细信息

我们已构建两组质粒,适合于在大肠杆菌内克隆基因和直接大量合成融合蛋白。这些质粒含有***的lac启动基因和部分编码β-半乳糖苷酶的序列,编码的蛋白长度约590或450个氨基酸残基(原基因编码1023个氨基酸残基)。被缩短的β-半乳糖苷酶基因的末端跟随一个多种限制性酶的接头序列;这个序列可被八种限制性酶中的任一种切断,然后插入任何蛋白基因。每组质粒有三种,可以满足所有三种不同的翻译读框。我们克隆了人工合成的人胰岛素原基因到这些质粒上去,在大肠杆菌内可以生产大量β-半乳糖苷酶残基——人胰岛素原融合蛋白。

关键词： BCA gene 大肠杆菌大肠埃希氏菌埃希氏杆菌属融合蛋白蛋白质质粒基因载体

Neocosmopolitan distributions of invertebrate aquatic invasive species due to euryhaline geographic history and human-mediated dispersal: Ponto-Caspian versus other geographic origins

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Ecological Processes 2023年第1期12卷 15-30页

作者： Aldona Dobrzycka-Krahel Carol A.stepien Zofia Nuc WSB University in Gdańsk Faculty of Oceanography and Geography Institute of Oceanography University of Gdansk Department of Vertebrate Zoology Smithsonian Institution National Museum of Natural History

Background: Aquatic invertebrate species that have broad salinity tolerances may be pre-adapted for invasion success and biogeographic distributional range expansions, facilitated by human-mediated dispersal(HMD), leading to a trend to become neocosmopolitan across many regions of the world. This pattern appears to characterize many Ponto-Caspian(P-C) aquatic invertebrates, which have a > 100-year history as aquatic invasive species(AIS), spreading throughout much of Eurasia and for some, in North America and beyond. Our study compiles comparative salinity conditions and distributional data for AIS invertebrate species globally versus those originating from the P-C region, to test whether they statistically ***: Our investigation discerns that a total of 1861 invertebrate AIS taxa have been recorded worldwide, with(A)70.5% exclusively living in the saline adaptive zone of brackish(0.5–30 ppt; A1) and/or marine waters(> 30 ppt; A2),(B) 20% in the freshwater adaptive zone alone(0–0.5 ppt),(C) 7.5% being euryhaline(across both A and B), and(D) 2%being semi-aquatic in either(D1) freshwater/terrestrial or(D2) saline/terrestrial environments. In contrast, our results indicate the following proportions for AIS invertebrates of P-C origins:(A) 27% exclusively inhabit the saline adaptive zone,(B) 25% are entirely freshwater,(C) 45% are euryhaline, and(D) 3% are semi-aquatic, significantly differing from the global pattern. Euryhaline AIS native to the P-C region thus markedly outnumber(45%) those originating from other regions(7.5%), likely pre-adapting them for widespread establishment in harbors, estuaries, and coastal ***, most P-C invertebrate AIS(70%) contain freshwater-tolerant populations(B + C), rendering them very successful invaders of inland water bodies. These broad salinity tolerances of P-C AIS underlie their tremendous invasion successes and growing neocosmopolitan distributions with ***: An evolutionary and recent history of

关键词： Aquatic invasive species Biogeography Distribution pattern Euryhalinity Freshwater adaptive zone Invasive species Neocosmopolitan Nonindigenous species Saline adaptive zone Zebra mussel

Beneficial impact of lithium bis(oxalato)borate as electrolyte additive for high-voltage nickel-rich lithium-battery cathodes

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InfoMat 2023年第8期5卷 67-80页

作者： Fanglin Wu Angelo Mullaliu Thomas Diemant Dominik stepien Tatjana NParac-Vogt Jae-Kwang Kim Dominic Bresser Guk-Tae Kim Stefano Passerini Helmholtz Institute Ulm(HIU) UlmGermany Karlsruhe Institute of Technology(KIT) KarlsruheGermany Department of Chemistry KU LeuvenLeuvenBelgium Department of Energy Convergence Engineering Cheongju UniversityCheongjuRepublic of Korea Chemistry Department Sapienza University of RomeRomeItaly

High-voltage nickel-rich layered cathodes possess the requisite,such as excellent discharge capacity and high energy density,to realize lithium batteries with higher energy ***,such materials suffer from structural and interfacial instability at high voltages(>4.3 V).To reinforce the stability of these cathode materials at elevated voltages,lithium borate salts are investigated as electrolyte additives to generate a superior cathode-electrolyte ***,the use of lithium bis(oxalato)borate(LiBOB)leads to an enhanced cycling stability with a capacity retention of 81.7%.Importantly,almost no voltage hysteresis is detected after 200 cycles at *** outstanding electrochemical performance is attributed to an enhanced structural and interfacial stability,which is attained by suppressing the generation of micro-cracks and the superficial structural degradation upon *** improved stability stems from the formation of a fortified borate-containing interphase which protects the highly reactive cathode from parasitic reactions with the ***,the decomposition process of LiBOB and the possible adsorption routes to the cathode surface are deduced and elucidated.

关键词： cathode electrolyte interphase electrolyte additive high voltage cathodes LiBOB nickel-rich cathodes

On the Consistency of the Arithmetic System

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Journal of Mathematics and System Science 2017年第2期7卷 43-55页

作者： T. J. stepien, L. T. Stepiefi The Pedagogical University of Cracow ul. Podchorazych 2 30 - 084 Krakow Poland

DYNAMIC DISORDER OF CADMIUM IN Cd2Nb2O7

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DYNAMIC DISORDER OF CADMIUM IN Cd2Nb2O7

Biomass Gasification and Syngas Production for Use in Solid Oxide Fuel Cell: Gas Cleaning and Conditioning, and Performance Tests

Sixteenth Congress of the International Union of Crystallography

作者： K.Lukaszewicz A.Pietraszko J.stepien-Damm N.N.Kolpakova Institute of Low Temperature and Structure Research Polish Academy of Sciences50-950 Wroclaw P.O.Box 937Poland

＜正＞Cadmium nlobate undergoes numerous phase transitions with minute or almost non-observable changes of the crystal structure（Ye,Z.G.,Kolpakova,N.N.,Rivera, J.-*** Schmid,H.,Ferroelectrlcs,1991,144,275- 280）.The crystal structure has been redetermined at RT, 350K and *** crystal structure analysis proved considerable anisotropy of cadmium temperature factors. In good agreement with x-ray diffraction data,this

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Biomass Gasification and Syngas Production for Use in Solid ...

On the Inconsistency of Classical Propositional Calculus

The 20th European Biomass Conference and Exhibition

作者： Jewulski,J. stepien,M. Blesznowski,M. Gallucci,K. Barisano,D.

Performance tests have been completed with a SOFC unit fuelled with syngas produced from biomass gasification reactors, properly conditioned and cleaned. Three series of tests at bench and industrial scale have been completed. The results of the tests indicate that cleaned syngas, from both steam blown and oxygen/steam blown gasification reactors, is a feasible fuel for anode supported SOFC cell. Cell performance on cleaned syngas is comparable or better than SOFC cell performance fuelled with the reference, hydrogen/nitrogen gas mixture. Several potential issues for the integrated SOFC/gasifier operation have been identified. Proper humidification of the gas stream, necessary to avoid carbon deposition, is critical for the long term cell performance. The presence of larger amounts of methane and higher hydrocarbons may lead to SOFC cell cracking due to large temperature gradients resulting from internal reforming process.

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Journal of Mathematics and System Science 2020年第1期10卷 13-14页

作者： Teodor J.stepien Lukasz T.stepien The Pedagogical University of Cracow Krakowul.Podchorazych230-084Poland

The classical propositional calculus(often called also as“zero-order logic”),is the most fundamental two-valued logical *** is necessary to construct the classical calculus of quantifiers(often called also as“classical calculus of predicates”or“first-order logic”),which is necessary to construct the classical functional *** last one is being used for formalization of the Arithmetic *** the beginning of this paper,we introduce a notation and we repeat certain well-known notions(among others,the notions of operation of consequence,a system,consistency in the traditional sense,consistency in the absolute sense)and certain well-known ***,we establish that classical propositional calculus is an inconsistent theory.

关键词： Classical propositional calculus consistency in the traditional sense consistency in the absolute sense

Atomic Entailment and Atomic Inconsistency and Classical Entailment

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Journal of Mathematics and System Science 2015年第2期5卷 60-71页

作者： T. J. stepien L. T. stepien The Pedagogical University of Cracow ul. Podchorazych 2 30 - 084 Krakow Poland

In this paper we put forward a new solution of the well-known problem of relevant logics, i. e., we construct an atomic entailment. Hence, we construct a system of predicate calculus based on the atomic entailment. Next, we establish the definition of atomic inconsistency. The atomic inconsistency establishes an infinite class of inconsistent, but non-trivial systems. In this paper we construct the new definition of the classical entailment, into the bargain.

关键词：原子蕴涵不一致性古典相关逻辑谓词演算无限类构造

The Formalization of The Arithmetic System on The Ground of The Atomic Logic

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Journal of Mathematics and System Science 2015年第9期5卷 364-368页

作者： T. J. stepien L. T. stepien The Pedagogical University of Cracow ul. Podchorazych 2 30 - 084 Krakow Poland.

This paper is a continuation of the paper [16]. Namely, in [16] we have introduced, among others, the definition of the atomic entailment and we have constructed the system s?, which is based on the atomic entailment.... 详细信息

关键词：运算系统原子逻辑蕴涵算术