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Liver steatosis correlates with iron overload but not with HFE gene mutations in chronic hepatitis C

Hepatobiliary & Pancreatic Diseases International 2013年第4期12卷 377-384页

作者： Katarzyna Sikorska piotr stalke Tomasz Romanowski Robert Rzepko Krzysztof piotr Bielawski Department of Infectious Diseases Medical University of GdanskSmoluchowskiego 18 80-214 Gdansk Poland Department of Biotechnology Intercollegiate Faculty of Biotechnology University of Gdansk and Medical University of GdanskKladki 24 80-822 Gdansk Poland Department of Pathology Medical University of GdanskDebinki 7 80-952 Gdansk Poland

BACKGROUND: Liver steatosis and iron overload, which are frequently observed in chronic hepatitis C (CHC), may contribute to the progression of liver injury. This study aimed to evaluate the correlation between liver steatosis and iron overload in Polish patients with CHC compared to non- alcoholic fatty liver disease (NAFLD) and HFE-hereditary hemochromatosis (HH) patients. METHODS: A total of 191 CHC patients were compared with 67 NAFLD and 21 HH patients. Liver function tests, serum markers of iron metabolism, cholesterol and triglycerides were assayed. The inflammatory activity, fibrosis, iron deposits and steatosis stages were assessed in liver specimens. HFE gene polymorphisms were investigated by PCR-RFLP. RESULTS: Liver steatosis was associated with obesity and diabetes mellitus. This disease was confirmed in 76/174 (44%) CHC patients, most of whom were infected with genotype 1. The average grade of steatosis was higher in NAFLD patients. CHC patients had significantly higher iron concentrations and transferrin saturations than NAFLD patients. Compared with CHC patients, HH patients had higher values of serum iron parameters and more intensive hepatocyte iron deposits without differences in the prevalence and intensity of liver steatosis. In the CHC group, lipids accumulation in hepatocytes was significantly associated with the presence of serummarkers of iron overload. No correlation between the HFE gene polymorphism and liver steatosis in CHC patients was found. CONCLUSIONS: Liver steatosis was diagnosed in nearly half of CHC patients, most of whom were infected with genotype 1. The intensity of steatosis was lower in CHC patients than that in NAFLD patients because of a less frequent diagnosis of metabolic syndrome. Only in CHC patients were biochemical markers of iron accumulation positively correlated with liver steatosis; these findings were independent of HFE gene mutations.

关键词： hepatitis C virus iron overload fatty liver hemochromatosis metabolic syndrome X

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Iron overload and HFE gene mutations in Polish patients with liver cirrhosis

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Hepatobiliary & Pancreatic Diseases International 2011年第3期10卷 270-275页

作者： Katarzyna Sikorska piotr stalke Tomasz Romanowski Ewa Izycka-Swieszewska Krzysztof piotr Bielawski Department of Infectious Diseases Medical University of Gdanskul.Smoluchowskiego 1880-218 GdanskPoland Department of Biotechnology Intercollegiate Faculty of BiotechnologyUniversity of Gdansk and Medical University of Gdanskul.Kladki 2480-822 GdańskPoland Department of Pathology Medical University of Gdanskul.Debinki 780-952 GdanskPoland

BACKGROUND:Increased liver iron stores may contribute to the progression of liver injury and fibrosis,and are associated with a higher risk of hepatocellular carcinoma ***-transplant symptoms of iron overload in patients with liver cirrhosis are associated with higher risk of infectious and malignant complications in liver transplant *** gene mutations may be involved in the pathogenesis of liver iron overload and influence the progression of chronic liver diseases of different *** study was designed to determine the prevalence of iron overload in relation to HFE gene mutations among Polish patients with liver ***:Sixty-one patients with liver cirrhosis included in the study were compared with a control group of 42 consecutive patients subjected to liver biopsy because of chronic liver *** function tests and serum iron markers were assessed in both *** patients were screened for HFE mutations (C282Y,H63D,S65C).Thirty-six of 61 patients from the study group and all controls had liver biopsy performed with semiquantitative assessment of iron deposits in ***:The biochemical markers of iron overload and iron deposits in the liver were detected with a higher frequency (70% and 47% respectively) in patients with liver *** were no differences in the prevalence of all HFE mutations in both *** patients with a diagnosis of hepatocellular carcinoma,no significant associations with iron disorders and HFE gene mutations were ***:Iron disorders were detected in patients with liver cirrhosis frequently but without significant association with HFE gene *** the homozygous C282Y mutation seems to occur more frequently in the selected population of patients with liver *** elevated biochemical iron indices accompanied liver iron deposits more frequently in liver cirrhosis compared to controls with chronic liver disease,there is a need for more extensive s

关键词： liver cirrhosis iron overload gene mutations iron deposits hepatocytes

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应用PCR-变性梯度凝胶电泳与免疫组化法检测慢性肝病患者肝脏与胃组织中的幽门螺杆菌

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世界核心医学期刊文摘（胃肠病学分册） 2006年第2期2卷 58-59页

作者： stalke P. Al-Soud W.A. Bielawski K.P. 成虹 Department of Infectious Disease Medical University of Gdansk ul. Smoluchowskiego 18 80- 214 Gdansk Poland Dr.

Objective. Helicobacter DNA has been detected in the hepatobiliary tree of patients with chronic liver diseases (CLD). The presence of H. pylori in the stomach compared with in the liver of the same patients with CLD has not been studied, therefore to the aim of this study was to investigate the presence of Helicobacter DNA and antigens in the liver and stomach of Polish patients with chronic liver diseases using molecular and immunological methods. Material and methods. Gastric mucosa and liver tissue samples and sera were collected from 97 Polish patients with CLD. Anti-H. pylori antibodies were detected by enzyme immunoassay (EIA), and H. pylori like antigens detected by immunohistochemistry. Helicobacter DNA was detected in stomach and liver samples using a seminested Helicobacter genus-specific polymerase chain reaction (PCR) assay, and Helicobacter species identified by denaturing gradient gel electrophoresis (DGGE) and sequencing analysis of amplified PCR products. Results. H. pylori was identified by DGGE and sequence analysis in 60/62 (97% ) and 25/25 (100% ) of the gastric and liver Helicobacter genus-positive samples, respectively, whereas DNA of H. heilmannii was detected in 2/62 (3% ) of the Helicobacter genus-positive gastric samples. H. pylori cagA gene was detected in 23/62 (36% ) and 3/25 (12% ) gastric and liver tissue samples, respectively. H. pylori-like antigens were detected in 61/97 (63% ) gastric mucosa and in 40/97 (41% ) liver tissue samples. Conclusions. H. pylori-like organisms appeared to dominate the gastric mucosa and liver tissue of Polish patients with CLD. The prevalence of the cagA gene was higher in stomach compared with liver samples, which suggests a possible role of cagA negative H. pylori-like organisms in CLD. On the other hand, no significant correlation was found between the presence of H. pylori-like DNA and antigens in the liver and liver function tests.

关键词：慢性肝病患者 PCR 免疫组化法胃组织海尔曼螺杆菌活检标本酶免疫测定肝胆管免疫学方法肝

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