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Isolation and microbial transformation of tea sapogenin from seed pomace of Camellia oleifera with anti-inflammatory effects

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Chinese Journal of Natural Medicines 2024年第3期22卷 280-288页

作者： shen pingping JIANG Xuewa ZHANG Jingling WANG Jiayi Raj Richa LI Guolong GE Haixia WANG Weiwei YU Boyang ZHANG Jian State Key Laboratory of Natural Medicines China Pharmaceutical UniversityNanjing 210009China Co-construction Collaborative Innovation Center for Chinese Medicine Resources Industrialization by Shaanxi&Education Ministry Shaanxi Province Key Laboratory of New Drugs and Chinese Medicine Foundation ResearchShaanxi University of Chinese MedicineXianyang 712046China School of Life Sciences Huzhou UniversityHuzhou 313000China Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine Nanjing 210046China Jiangsu Key Laboratory of TCM Evaluation and Translational Research China Pharmaceutical UniversityNanjing 211198China

In the current study,tea saponin,identified as the primary bioactive constituent in seed pomace of Camellia oleifera Abel.,was meticulously extracted and hydrolyzed to yield five known sapogenins:16-O-tiglogycamelliagnin B(a),camelliagnin A(b),16-O-angeloybarringtogenol C(c),theasapogenol E(d),theasapogenol F(e).Subsequent biotransformation of compound a facilitated the isolation of six novel metabolites(a1−a6).The anti-inflammatory potential of these compounds was assessed using pathogenassociated molecular patterns(PAMPs)and damage-associated molecular patterns molecules(DAMPs)-mediated cellular inflammation models.Notably,compounds b and a2 demonstrated significant inhibitory effects on both lipopolysaccharide(LPS)and high-mobility group box 1(HMGB1)-induced inflammation,surpassing the efficacy of the standard anti-inflammatory agent,carbenoxolone.Conversely,compounds d,a3,and a6 selectivity targeted endogenous HMGB1-induced inflammation,showcasing a pronounced specificity.These results underscore the therapeutic promise of C.oleifera seed pomace-derived compounds as potent agents for the management of inflammatory diseases triggered by infections and tissue damage.

关键词： Camellia oleifera Seed pomace Tea sapogenin Microbial transformation Damage-associated molecular patterns and Pathogen-associated molecular patterns Anti-inflammatory activity

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Factors inducing morphological transition in harmful algal bloom-forming species Phaeocystis globosa with emphasis on predator chemical cue

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Journal of Oceanology and Limnology 2023年第4期41卷 1537-1546页

作者： Xiao YANG pingping shen Ke LI Yantai Institute of Coastal Zone Research Chinese Academy of SciencesYantai 264003China Ocean School Yantai UniversityYantai 264005China College of Resources and Environment University of Chinese Academy of SciencesBeijing 100049China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China

The microalga Phaeocystis globosa is recognized as a harmful alga and also one of the few keystone phytoplankton genera that shape the structure and function of marine ecosystems.P.globosa possess a complex polymorphic life cycle,exhibiting phase alternation between free-living cells of approximately 3-6μm in diameter and gelatinous colonies(palmelloid stage)reaching several millimeters.The knowledge on the factors that induced the morphological transition of P.globosa in the last two decades was reviewed.Emphasis is given to infochemicals,an additional biological factor induced by predator,with the attempt to reveal a relevant mechanism of induced morphological defense.

关键词： Phaeocystis globosa polymorphisms harmful algal blooms defense strategies infochemical

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Diversity and geographical distribution of haptophyte Phaeocystis in the Chinese seas based on metabarcoding analysis

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Journal of Oceanology and Limnology 2023年第6期41卷 2197-2207页

作者： Qingshang SONG Yue XUE Yanying ZHANG Jiehui YIN pingping shen Ocean School Yantai UniversityYantai 264005China

Phaeocystis is an important bloom-forming species and over 100 blooms have occurred since 1997 along the Chinese coasts,while only the species P.globosa was described.In this project,a total of 246 phytoplankton samples collected from the Bohai Sea,the Yellow Sea,and the East China Sea in April 2021 were analyzed to evaluate the species diversity and geographical distribution of the genus Phaeocystis based on metabarcoding 18S r DNA sequence.Four described Phaeocystis species were recognized,including P.globosa,P.pouchetii,P.jahnii,and P.cordata,and each has distinguished geographical distribution characteristics.P.globosa was the most widespread and abundant species,and P.pouchetii was found in the Bohai Sea and the northern Yellow Sea with substantially elevated abundance.P.cordata was also a widespread species,but its abundance was relatively low,while P.jahnii gathered only in the southeastern East China Sea.Water temperature,phosphate as well as ammonium contents were found to be associated with the abundance of P.globosa,P.pouchetii,and P.jahnii significantly.Moreover,two uncharacterized Phaeocystis species were detected in the Chinese seas,indicating the diversity of the genus remains underestimated worldwide.

关键词： Haptophyta Phaeocystis species diversity distribution Chinese seas amplicon sequence variant

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Crosstalk between IL-15Rα^(+)tumor-associated macrophages and breast cancer cells reduces CD8+T cell recruitment

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Cancer Communications 2022年第6期42卷 536-557页

作者： Wenlong Zhang Qing Zhang Nanfei Yang Qian Shi Huifang Su Tingsheng Lin Zhonglei He Wenxin Wang Hongqian Guo pingping shen State Key Laboratory of Pharmaceutical Biotechnology and Department of Urology Nanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical SchoolSchool of Life SciencesNanjing UniversityNanjingJiangsu 210023P.R.China Department of Urology Drum Tower HospitalMedical School of Nanjing UniversityInstitute of UrologyNanjing UniversityNanjingJiangsu 210008P.R.China shenzhen Research Institute of Nanjing University Shenzhen 518000China Department of Cellular and Integrative Physiology The University of Texas Health Science Center at San AntonioSan AntonioTexas 78229-3904USA Charles Institute of Dermatology School of MedicineUniversity College DublinDublin Eircode D04 V1W8Ireland

Background:Interleukin-15(IL-15)is a promising immunotherapeutic agent owing to its powerful immune-activating effects.However,the clinical benefits of these treatments are limited.Crosstalk between tumor cells and immune cells plays an important role in immune escape and immunotherapy drug resistance.Herein,this study aimed to obtain in-depth understanding of crosstalk in the tumor microenvironment for providing potential therapeutic strategies to prevent tumor progression.Methods:T-cell killing assays and co-culture models were developed to determine the role of crosstalk between macrophages and tumor cells in breast cancer resistant to IL-15.Western blotting,histological analysis,CRISPR-Cas9 knockout,multi-parameter flow cytometry,and tumor cell-macrophage co-injection mouse models were developed to examine the mechanism by which IL-15Rα^(+)tumor-associated macrophages(TAMs)regulate breast cancer cell resistance to IL-15.Results:We found thatmacrophages contributed to the resistance of tumor cells to IL-15,and tumor cells induced macrophages to express high levels of theαsubunit of the IL-15 receptor(IL-15Rα).Further investigation showed that IL-15Rα^(+)TAMs reduced the protein levels of chemokine CX3C chemokine ligand 1(CX3CL1)in tumor cells to inhibit the recruitment of CD8^(+)T cells by releasing the IL-15/IL-15Rαcomplex(IL-15Rc).Administration of an IL-15Rc blocking peptide markedly suppressed breast tumor growth and overcame the resistance of cancer cells to anti-programmed cell death protein 1(PD-1)antibody immunotherapy.Interestingly,Granulocyte-macrophage colony-stimulating factor(GMCSF)inducedγchain(γc)expression to promote tumor cell-macrophage crosstalk,which facilitated tumor resistance to IL-15.Additionally,we observed that the non-transcriptional regulatory function of hypoxia inducible factor-1alpha(HIF-1α)was essential for IL-15Rc to regulateCX3CL1 expression in tumor cells.Conclusions:The IL-15Rc-HIF-1α-CX3CL1 signaling pathway serves as a crosstalk between macrophages and tumor cells in the t

关键词： breast cancer CD8^(+)T-cell crosstalk IL-15Rα immunotherapy tumor microenvironment tumor-associated macrophages

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Intervening pyruvate carboxylase stunts tumor growth by strengthening anti-tumor actions of tumor-associated macrophages

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Signal Transduction and Targeted Therapy 2022年第3期7卷 607-609页

作者： Yuxin Shu Nanfei Yang Nan Cheng Zhengyun Zou\Wenlong Zhang Yuncheng Bei Qian Shi Menghao Qin Wei-Guo Zhu pingping shen State Key Laboratory of Pharmaceutical Biotechnology and The Comprehensive Cancer Center Nanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical SchoolSchool of Life SciencesNanjing UniversityNanjing 210023PR Chino Guangdong Key Laboratory of Genome Instability and Human Disease Shenzhen University International Cancer CenterDepartment of Biochemistry and Molecular BiologyShenzhen UniversityShenzhen 518060PR China Department of Cellular and Integrative Physiology The University of Texas Health Science Center at San Antonio7703 Floyd Curl DriveSan AntonioTX 78229-3904USA

Dear Editor,Tumor-associated macrophages(TAMs)are critical pro-tumor immunocytes and depletion of TAMs has been exploited for cancer therapy.1 However,the phenotypes and functions of TAMs are plastic,TAMs can also be effector cells by engulfing tumor cells and recruiting cytotoxic T cells,thus shaping the actions of TAMs is more scientific rational than depleting them indiscriminately.2 To promote the entry of reorienting TAMs into the clinical treatments of tumors,it is essential to explore the underline mechanisms controlling the anti-and pro-tumor activities of TAMs.

关键词： strengthening clinical rational

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A novel mesenchymal stem cell-based regimen for acute myeloid leukemia differentiation therapy

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药学学报:英文版 2023年第7期13卷 3027-3042页

作者： Luchen Sun Nanfei Yang Bing Chen Yuncheng Bei Zisheng Kang Can Zhang Nan Zhang Peipei Xu Wei Yang Jia Wei Jiangqiong Ke Weijian Sun Xiaokun Li pingping shen The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University Wenzhou 325027China School of Pharmaceutical Science Wenzhou Medical UniversityWenzhou 325035China Department of Urology Drum Tower HospitalMedical School of Nanjing UniversityInstitute of UrologyNanjing UniversityNanjing 210008China The Comprehensive Cancer Centre of Nanjing Drum Tower Hospital the Affiliated Hospital of Nanjing University Medical SchoolNanjing UniversityNanjing 210008China Department of Hematology the Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjing 210093China State Key Laboratory of Natural Medicines and Jiangsu Key Laboratory of Drug Discovery for Metabolic Diseases Center of Drug DiscoveryChina Pharmaceutical UniversityNanjing 210009China Centre of Micro/Nano Manufacturing Technology(MNMT-Dublin) School of Mechanical&Materials EngineeringUniversity College DublinDublin 4Ireland Department of Surgery Division of Cancer Biology and TherapeuticsCedars-Sinai Medical CenterLos AngelesCA 90048USA Oujiang Laboratory(Zhejiang Lab for Regenerative Medicine Vision and Brain Health)&School of Pharmaceutical ScienceWenzhou Medical UniversityWenzhou 325035China shenzhen Research Institute of Nanjing University Shenzhen 518000China

Currently the main treatment of acute myeloid leukemia(AML)is chemotherapy combining hematopoietic stem cell transplantation.However,the unbearable side effect of chemotherapy and the high risk of life-threatening infections and disease relapse following hematopoietic stem cell transplantation restrict its application in clinical practice.Thus,there is an urgent need to develop alternative therapeutic tactics with significant efficacy and attenuated adverse effects.Here,we revealed that umbilical cord-derived mesenchymal stem cells(UC-MSC)efficiently induced AML cell differentiation by shuttling the neutrophil elastase(NE)-packaged extracellular vesicles(EVs)into AML cells.Interestingly,the generation and release of NE-packaged EVs could be dramatically increased by vitamin D receptor(VDR)activation in UC-MSC.Chemical activation of VDR by using its agonist 1a,25-dihydroxyvitamin D3 efficiently enhanced the pro-differentiation capacity of UC-MSC and then alleviated malignant burden in AML mouse model.Based on these discoveries,to evade the risk of hypercalcemia,we synthetized and identified sw-22,a novel non-steroidal VDR agonist,which exerted a synergistic prodifferentiation function with UC-MSC on mitigating the progress of AML.Collectively,our findings provided a non-gene editing MSC-based therapeutic regimen to overcome the differentiation blockade in AML.

关键词： Mesenchymal stem cell Acute myeloid leukemia Extracellular vesicles Nonsteroidal VDR modulators Combination therapy

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Research on the New Modern English Teaching Mode in Higher Vocational Colleges based on Multimedia and Interactive System

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International Journal of Technology Management 2016年第7期 54-56页

作者： pingping shen Shandong Foreign Languages Vocational College Rizhao CityShandong Province276826 China

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气体流量计量虚拟仿真教学项目设计

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实验室研究与探索 2024年第5期43卷 68-71,115页

作者：沈平平赵伟国赵鹏梁佳娜梁晓瑜中国计量大学计量测试工程学院杭州310018 浙江方圆检测集团股份有限公司杭州310018

气体流量计量标准装置结构复杂、价格昂贵,在传统实验教学模式下学生难以开展动手操作,为此开发气体流量计量的虚拟仿真实验。运用虚拟仿真技术搭建音速喷嘴气体流量传递标准装置,在虚拟三维场景中学生可对气体流量标准装置的结构和工... 详细信息

气体流量计量标准装置结构复杂、价格昂贵,在传统实验教学模式下学生难以开展动手操作,为此开发气体流量计量的虚拟仿真实验。运用虚拟仿真技术搭建音速喷嘴气体流量传递标准装置,在虚拟三维场景中学生可对气体流量标准装置的结构和工作原理展开全方位的认知,掌握气体流量量值传递与溯源方法。同时依据检定规程设计气体涡轮流量计检定交互步骤,使学生掌握涡轮流量计的检定流程及其计量性能评价方法。该项目克服了传统流量计量实验教学中存在的难题,取得了较好的实验教学效果,有效提升学生的专业认知和行业从业能力。

关键词：气体流量计量虚拟仿真实验教学

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聚焦超声温度分布实验测量方法研究

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中国测试 2024年第5期50卷 86-92页

作者：石婉婷赵鹏王月兵徐佳奇谢鹏飞沈平平中国计量大学浙江杭州310018

针对医疗领域中聚焦超声场温度分布测量操作繁琐、条件复杂等问题,研制一种柔性膜式温度阵列传感器及测量方法。利用PTC效应实现温度测量,基于聚乙烯高分子基薄膜研制温度传感器阵列,基于多通道快速采集系统搭建恒温水浴标定系统,配制... 详细信息

针对医疗领域中聚焦超声场温度分布测量操作繁琐、条件复杂等问题,研制一种柔性膜式温度阵列传感器及测量方法。利用PTC效应实现温度测量,基于聚乙烯高分子基薄膜研制温度传感器阵列,基于多通道快速采集系统搭建恒温水浴标定系统,配制仿生体模,并进行聚焦超声温度分布实验。标定结果表明:该传感器可以覆盖6 mm测温范围,空间分辨率为0.4 mm,测量重复性为2.4%以内,可用于聚焦超声温度分布的测量。与仿真及热电偶测量结果比较表明,焦点最高温度偏差在1.25℃以内。基于高分子基薄膜的聚焦超声场温度分布测量方法是一种精度高、操作简单的测温方法。

关键词：阵列传感器聚焦超声温度分布高分子基膜

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山东沿海红纤维虾形草根际微生物群落结构及功能

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烟台大学学报（自然科学与工程版） 2024年第1期37卷 26-36页

作者：张燕英王天雨孙浩沈萍萍烟台大学海洋学院山东烟台264005

采用高通量扩增子测序技术和宏基因组测序技术,探究俚岛和长岛海域红纤维虾形草(Phyllospadix iwatensis)根际细菌、原生生物和真菌群落结构特征和潜在功能。结果表明,细菌的多样性和丰度显著高于原生生物和真菌,且细菌互作网络更复杂,... 详细信息

采用高通量扩增子测序技术和宏基因组测序技术,探究俚岛和长岛海域红纤维虾形草(Phyllospadix iwatensis)根际细菌、原生生物和真菌群落结构特征和潜在功能。结果表明,细菌的多样性和丰度显著高于原生生物和真菌,且细菌互作网络更复杂,俚岛和长岛海域的微生物群落结构存在显著差异。细菌以拟杆菌纲(Bacteroidia)、γ-变形菌纲(Gammaproteobacteria)、Desulfobulbia和α-变形菌纲(Alphaproteobacteria)为优势类群。原生生物群落组成差异较大,未鉴定的原生生物占比较高,俚岛优势类群为类锥体纲(Conoidasida),而长岛优势类群为石莼纲(Ulvophyceae)和褐藻纲(Phaeophyceae)。两个海域真菌群落均以粪壳菌纲(Sordariomycetes)为优势类群。功能分析揭示参与红纤维虾形草根际氮循环和硫循环的微生物共分布于17个属中,其中荧光杆菌属(Ilumatobacter)、硫卵菌属(Sulfurovum)和甲基海洋杆菌属(Methyloceanbacter)在两个海域的相对丰度存在显著差异,这三个微生物类群对海草根际硫酸盐还原和脱氮过程贡献度较高。所得数据可为挖掘微生物资源用于海草床生态系统的修复提供理论依据。

关键词：海草细菌真菌原生动物群落结构宏基因组学

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