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Methylenetetrahydrofolate Reductase Genotypes, Dietary Habits and Susceptibility to Stomach Cancer

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Chinese Journal of Clinical Oncology 2004年第3期1卷 162-166页

作者： ChangmingGao TakezakiToshiro JianzhongWu JianhuoDing YantingLiu SupingLi pingsu XuHu TianliongXu HamajimaNobuyuki TajimaKazuo DivisionofEpidemiology JiangsuProvinceInstituteofCancerResearchNanjing210009China. DivisionofEpidemiologyandPrevention AichiCancerCenterResearchInstituteNagoya464-8681Japan. PublicHealthCenterofChuzhouDistrict Huaian221300China.

OBJECTIVE To study the relation among methylenetetrahydrofolate reductase (MTHFR) C677T genotypes, dietary habits and the risk of stomach cancer (SC).METHODS A case-control study was conducted with 107 cases of SC and 200 population-based controls in Chuzhou district, Huaian, Jiangsu province, China. The epidemiological data were collected, and DNA of peripheral blood leukocytes was obtained from all of the subjects..MTHFR genotypes were detected by PCR-RFLP. RESULTS (1) The prevalence of the MTHFR C/T or T/T genotypes was found to be significantly different between controls (68.5%) and SC cases (79.4%,P=0.0416), the increased risk had an adjusted OR of 1.79 (95%C1:1.01-3.19). (2) Among subjects who had a low intake of garlic or Chinese onion, MTHFR C/T or T/T genotypes significantly increased the risk of developing SC. Among non-tea drinkers or among subjects who had a frequent intakeof meat, the carriers of the MTHFR C/T or T/T genotypes had a higher risk of SC than individuals with the C/C type MTHFR. CONCLUSION The polymorphism of MTHFR C677T was associated with increased risk of developing SC, and that individuals with differing genotypes may have different susceptibilities to SC, based on their exposure level to environmental factors.

关键词：亚甲基四氢叶酸还原酶基因型饮食习惯易感性胃癌肿瘤

Cloning and expression of mouse peroxiredoxin I in 1EC-6 Cells

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World Journal of Gastroenterology 2004年第14期10卷 2109-2112页

作者： BoZhang Yong-pingsu TaoWang Feng-ChaoWang Guo-PingAi HuiXu Jun-PingWang Yue-ShengHuang Jian-XinJiang InstituteofCombinedInjuryofPLA StateKeyLaboratoryofTraumaBurnsandCombinedInjuryThirdMilitaryMedicalUniversityChongqing400038China

AIM: To clone and express mouse peroxiredoxin Ⅰ in IEC-6 ***: Total RNAs were isolated from cultured IEC-6 cells, and the coding region of peroxiredoxin I was amplified by RT-PCR. After it was cloned into T-vector and sequenced,pSG5 was used to transiently express peroxiredoxin I in IEC-6 by liposome-mediated transfection, and the expression of peroxiredoxin I was evaluated by RT-PCRand Western blot. RESULTS: A DNA fragment about 750 bp was amplified from total RNAs of IEC-6 cells using specific primers of peroxiredoxin Ⅰ. The sequencing confirmed the coding region was successfully cloned into T-vector, which was completely coincident with the sequence in GeneBank. After the EcoRI-BamHI fragment of T-vector containing peroxiredoxin I was inserted into pSG5, the recombinant plasmid was transferred to IEC-6 cells. RT-PCR assay showed that a DNA fragment of 930 bp could be amplified,which indicated the transcription of pSG5-Prx. Western blot confirmed the expression of peroxiredoxin Ⅰ in IEC-6 ***: Mouse peroxiredoxin I can be successfully expressed in IEC-6 cells.

关键词：无性繁殖基因表达老鼠 peroxiredoxinⅠ IEC-6细胞重组器官

Protective role of metallothionein (Ⅰ/Ⅱ) against pathological damage and apoptosis induced by dimethylarsinic acid

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World Journal of Gastroenterology 2004年第1期10卷 91-95页

作者： GuangJia Yi-QunGu Kung-TungChen You-YongLu LeiYan Jian-LingWang Ya-pingsu J.C.GastonWu DepartmentofOccupationalandEnvironmentalHealthSciences SchoolofPublicHealthPekingUniversityBeijing100083China DepartmentofPathology HepingliHospitalBeijing100013China DepartmentofGeneralEducation Ming-hsinUniversityofScienceandTechnologyTaiwanChina SchoolofOncology PekingUniversityBeijing100034China DepartmentofChemistry NationalTaiwanNormalUniversityTaiwanChina

AIM: To better clarify the main target organs of dimethylarsinic acid toxicity and the role of metallothionein (MTs) in modifying dimethylarsinic acid (DMAA) toxicity. METHODS: MT-Ⅰ/Ⅱ null (MT^-/-) mice and the corresponding wild-type mice (MT^+/+), six in each group, were exposed to DMAA (0-750 mg/kg body weight) by a single oral *** four hours later, the lungs, livers and kidneys were collected and undergone pathological analysis, induction of apoptolic cells as determined by TUNEL and Mr concentration was detected by radio-immunoassay. RESULTS: Remarkable pathological lesions were observed at the doses ranging from 350 to 750 mg/kg body weight in the lungs, livers and kidneys and MT^+/+ mice exhibited a relatively slight destruction when compared with that in dose matched MT/ mice. The number of apoptotic cells was increased in a dose dependent manner in the lungs and livers in both types of mice. DMAA produced more necrotic cells rather than apoptotic cells at the highest dose of 750 mg/kg,however, no significant increase was observed in the *** Mr level in Mr^+/+ mice was significantly increased by DMAA in a dose-dependent manner and there was no detectable amount of hepatic MT in untreated MT^-/- mice. CONCLUSION: DMAA treatment can lead to the induction of apoptosis and pathological damage in both types of *** exhibits a protective effect against DMAA toxicity.

关键词：金属硫因二甲胂酸细胞凋亡病理形态学细胞毒性

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Siwear纤维的性能测试

棉纺织技术 2010年第5期38卷 9-11页

作者：张婷婷杨彩云秦平素天津工业大学河北科技大学

研究Siwear纤维的性能及其可纺性。测试了Siwear纤维的摩擦因数、强力、回潮率和质量比电阻,并与Outlast腈纶纤维和粘胶纤维进行了对比。指出:Siwear纤维的抱合性较好,强力和回潮率较高,但其质量比电阻较大,在纺纱过程中应采取相应措施... 详细信息

研究Siwear纤维的性能及其可纺性。测试了Siwear纤维的摩擦因数、强力、回潮率和质量比电阻,并与Outlast腈纶纤维和粘胶纤维进行了对比。指出:Siwear纤维的抱合性较好,强力和回潮率较高,但其质量比电阻较大,在纺纱过程中应采取相应措施以保证纺纱顺利进行。

关键词： Siwear纤维摩擦因数强力回潮率质量比电阻可纺性

番茄红素改善BTBR小鼠社交障碍及降低海马炎症

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陆军军医大学学报 2023年第14期45卷 1483-1492页

作者：李江辉李达兵朱先玉陈梅范晓棠毛苹苏西南医科大学基础医学院肿瘤医学研究所四川泸州646000 陆军军医大学(第三军医大学)医学心理系军事认知心理学教研室重庆400038 西南医科大学基础医学院基础医学院生理学教研室四川泸州646000

目的研究番茄红素(Lycopene,Lyc)对BTBR T^(+)Itpr3^(tf)/J(BTBR)自闭症模型小鼠海马组织中炎症水平、社交缺陷、认知障碍和重复刻板行为的影响。方法7~8周龄成年雄性健康C57BL/6(C57)与BTBR小鼠随机数字表法分为4组(n=8):C57+Veh组、C5... 详细信息

目的研究番茄红素(Lycopene,Lyc)对BTBR T^(+)Itpr3^(tf)/J(BTBR)自闭症模型小鼠海马组织中炎症水平、社交缺陷、认知障碍和重复刻板行为的影响。方法7~8周龄成年雄性健康C57BL/6(C57)与BTBR小鼠随机数字表法分为4组(n=8):C57+Veh组、C57+Lyc组、BTBR+Veh组、BTBR+Lyc组。C57+Lyc组和BTBR+Lyc组小鼠予以番茄红素(20 mg/kg)灌胃,1次/d,共7 d;C57+Veh组小鼠和BTBR+Veh组小鼠给予等体积的玉米油。给药7 d后开始三箱社交、雌雄交互、理毛、埋珠、开场实验、新物体识别、筑巢和Y迷宫等行为实验。结束后立即收取脑组织样本,进行RT-qPCR、Western blot实验。结果行为学结果显示:三箱社交实验中,BTBR+Veh组小鼠在陌生物体所在的箱体时间更多,表现出对陌生物体更偏好(P<0.05);而Lyc治疗后小鼠表现出对陌生小鼠更偏好(P<0.05)。理毛、埋珠实验中,BTBR+Veh组小鼠较C57+Veh组小鼠的重复刻板行为显著增多(P<0.05),Lyc治疗后显著减少了BTBR小鼠的重复刻板行为(P<0.05)。BTBR+Veh组小鼠较C57+Veh组小鼠海马组织中NLRP3炎症小体在mRNA和蛋白水平均显著增高(P<0.05);白细胞介素-1β(interleukin-1β,IL-1β)、IL-18、IL-6、核因子κB(nuclear factor kappaB,NF-κB)、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)在mRNA水平显著增高(P<0.05);Lyc治疗后BTBR小鼠各炎症因子均显著降低(P<0.05)。结论番茄红素治疗通过降低BTBR小鼠海马中NLRP3炎症小体的水平,改善BTBR小鼠三箱社交缺陷和重复刻板行为。

关键词：番茄红素自闭症 NLRP3炎症小体海马社交障碍

松辽盆地上白垩统高分辨率层序界面类型及特征

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大庆石油地质与开发 2014年第4期33卷 1-8页

作者：刘丽娟朱思源高有峰白雪峰刘平粟大庆油田有限责任公司勘探开发研究院黑龙江大庆163712 大庆油田有限责任公司第六采油厂黑龙江大庆163114 吉林大学古生物学与地层学研究中心吉林长春130061 中国地质大学北京100036

综合应用岩心、测井及露头剖面等资料,在松辽盆地上白垩统识别出古暴露面、冲刷面、岩性岩相转换面、同种岩性颜色变化界面等4种类型的层序界面,并描述了层序界面的野外露头、钻井岩心及测井曲线的识别特征.研究认为：大型冲刷面和长期... 详细信息

综合应用岩心、测井及露头剖面等资料,在松辽盆地上白垩统识别出古暴露面、冲刷面、岩性岩相转换面、同种岩性颜色变化界面等4种类型的层序界面,并描述了层序界面的野外露头、钻井岩心及测井曲线的识别特征.研究认为：大型冲刷面和长期、中长期暴露面可作为长期或中期基准面旋回的层序界面;中小型冲刷面、间歇暴露面、岩性—岩相转换面及颜色变化界面可作为短期或超短期基准面旋回的层序界面.

关键词：松辽盆地上白垩统高分辨率层序地层古暴露面冲刷面

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端粒DNA损伤修复机制及其意义

西南医科大学学报 2023年第1期46卷 87-92页

作者：朱先玉(综述) 李江辉(综述) 毛苹苏(审校) 西南医科大学基础医学院肿瘤医学研究所泸州646000

DNA损伤是细胞存活的主要威胁,若得不到恰当的修复,可能引起细胞衰老、死亡或者肿瘤的发生。真核生物线性染色体的末端保护依赖于端粒,端粒在维护基因组稳定性中具有重要的作用。端粒末端的特殊结构需要避免被识别为DNA损伤位点,从而避... 详细信息

DNA损伤是细胞存活的主要威胁,若得不到恰当的修复,可能引起细胞衰老、死亡或者肿瘤的发生。真核生物线性染色体的末端保护依赖于端粒,端粒在维护基因组稳定性中具有重要的作用。端粒末端的特殊结构需要避免被识别为DNA损伤位点,从而避免发生降解、重组以及末端连接。然而,端粒DNA富含鸟嘌呤,易受活性氧自由基攻击而产生损伤。细胞如何平衡末端保护与DNA损伤修复是一个重要的问题。本文将围绕端粒末端保护机制以及端粒DNA损伤修复最新研究进行综述。端粒随着细胞增殖而逐渐变短,最终引起细胞衰老。肿瘤细胞具有延伸端粒的能力,主要通过端粒酶或端粒延伸替代机制维持端粒长度。端粒在衰老以及肿瘤发生发展中扮演了重要的作用,本文还将总结端粒DNA损伤修复在衰老和肿瘤防治中的意义。

关键词：端粒末端保护 DNA损伤修复衰老肿瘤