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REGULAR EXPRESSION OF DISCOIDIN DOMAIN RECEPTOR 2 IN THE IMPROVED ADJUVANT-INDUCED ANIMAL MODEL FOR RHEUMATOID ARTHRITIS

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Chinese Medical Sciences Journal 2005年第2期20卷 133-137,页

作者： WeiLi Yuan-qiangZhang Xin-pingliu Li-boYao LanSun DepartmentofHistologyandEmbryology DepartmentofBiochemistryandMolecularBiology theFourthMilitaryUniversityXi＇an710032

Objective To investigate the expression of discoidin domain receptor 2 (DDR2) of fibroblast-like synovial cells in im- proved adjuvant-induced animal (AIA) model for rheumatoid arthritis (RA) and to provide evidence for DDR2’s antagonist use clinically. Methods AIA was modified by administrating 0.1 mL of complete Freund’s adjuvant (CFA, mixed with 5 mg Bacillus Calmette-Guerin vaccine/mL) into rats’ right hind paws and 0.125 mL tumor necrosis factor-α (2 U/mL) into right ankles and subpatellar fatty tissue. The expression of DDR2 in fibroblast-like synovial cells was assessed using immunohistochemistry, immunofluorescence histochemistry, and in situ hybridization methods. Levels of anti-collagen II antibody were measured using enzyme-linked immunosorbent assay. Results Given the terms mentioned above, we found a more practical rat model, apparently decreasing immunization time (average 3-5 days). DDR2 can be detected upon the 15th day of immunization; expression gradually increased with time going on, and reaching a peak 35 days after immunization before gradually decreasing. Serum anti-collagen II antibody showed similar expression patterns as DDR2, but reached peak later than DDR2, about 40 days after immunization. Conclusion Regular expression of DDR2 in animal models infers its important role in the pathological process of RA.

关键词： rheumatoid arthritis adjuvant-induced arthritis discoidin domain receptor 2 synovial tissue anti-collagen Ⅱ antibody

Spatio-Temporal Expression Pattern of Six Novel Candidate Genes in Ginsenoside Biosynthesis from Panax ginseng C. A. Meyer

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Journal of Integrative Plant Biology 2005年第5期47卷 622-631页

作者： Zhi-YongLUO Shui-pingliu Xiang-HuiCHEN YingRUAN Jian-QingLUO BinWEN Chun-LinLIU Wei-XinHU InstituteofMolecularBiology CollegeofBiologicalScienceandTechnologyCentralSouthUniversityChangsha410078China CropsGeneticEngineeringKeyLaboratoryofHunanProvince HunanAgriculturalUniversityChangsha410128China

Abstract: To explore the mode of the spatio-temporal expression of six newly discovered ginsenoside biosynthesis candidate gene transcripts, both Northern blotting and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) were used to elucidate the mRNA expression levels of the transcripts in various tissues and organs of Panax ginseng C. A. Meyer during different growth development stages. The six gene transcripts were all differentially expressed in cultured callus, root, stem, leaf, and seed. The mRNA expression levels were significantly higher in four-year-old roots than in one-year-old roots, and results of semi-quantitative RT-PCR assays were in accordance with those of Northern blotting analyses. The results strongly suggest that all six genes were differentially expressed at root-specific developmental stages. In particular, when a quiescent early stage culture suspension of P. ginseng cells was exposed to the ginsenoside biosynthesis-promoting elicitor Aspergillus niger polysaccharide, the GBR6 gene transcript response showed time-dependent increments and was parallel with ginsenoside productivity (P < 0.01). Overexpressionof the GBR6 gene is likely to play a critically important role in the biosynthesis of ginsenosides. The results of the present study provided a background for the further elucidation of the structure and physiological function of these six candidate genes.

关键词： Aspergillus niger polysaccharide elicitor GBR6 mRNA expression ginsenoside biosynthesis ginsenoside biosynthesis candidate genes mRNA differential expression Panax ginseng C. A. Meyer

Investigation on etretin effects on expression of Fas/FasL ligand and apoptosis in cultured human keratinocytes

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Journal of Nanjing Medical University 2005年第3期19卷 144-147页

作者： pingliu ShunshengTan YanpingXi ZhenpingCao DepartmentofDermatology theSecondHospitalXi＇anJiaotongUniversityXi＇an710004China DepartmentofDermatology theSecondHospitalXi＇anJiaotongUniversityXi＇an710004China

Objective:To further illuminate a possibme mechanism of Fas/FasL in the treatment of psoriasis, the expression of it and apoptosis of KC were investigated. Methods: With cell culture,immunocytochemistry, the expression of Fas/FasL protein after the treatment with etretin was observed in cultured human normal keratinocytes. Then, the state of apoptosis in cultured keratinocyte after stimulation with etretin was detected with FACS(Fluorescence Activited Cell Sortor). Results:① There was no expression of Fas/FasL protein in the cultured normal human KC. ②After the treatment with etretin, the strongest expression of FasL protein appeared after 16h,so did Fas after 40h.③ The higher the concentration of etretin,the stronger the expression of Fas/FasL *** the same condition,the expression of Fas is stronger than that of FasL.④ Keratinocytes' apoptosis occurred after stimulation with etretin. Conclusion: Fas/FasL system wasn't involved in apoptosis in cultured normol human *** during limited period, the apoptosis of KC could be induced by (etretin),thus it can antagonize benign proliferate of *** data showed up-regulation of the expression of Fas/FasL and apoptosis in cultured human keratinocytes stimulated by etretin, and its function may be involved in the therapeutic machanism of psoriasis.

关键词： etretin keratinocyte Fas FasL apoptosis

Grey scale enhancement of rabbit liver and kidney by intravenous injection of a new lipid-coated ultrasound contrast agent

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World Journal of Gastroenterology 2004年第16期10卷 2369-2372页

作者： pingliu Yun-HuaGao Kai-BinTan ZhengLiu SongZuo DepartmentofUltrasound XinqiaoHospitalThirdMilitaryMedicalUniversityChongqing400037China

AIM: To assess the grey scale enhancement of a new lipidcoated ultrasound contrast agent in solid abdominal organs as liver and ***: Size distribution and concentration of the lipidcoated contrast microbubbles were analyzed by a Coulter counter. Two-dimensional (2D) second harmonic imaging of the hepatic parenchyma, the inferior vena cava and the right kidney of the rabbits were acquired before and after contrast agent injection. Images were further quantified by histogram in Adobe Photoshop 6.0. Time-intensity curves of hepatic parenchyma, inferior vena cava and renal cortex were qenerated from the oriqinal qrev ***: The 2D images of hepatic parenchyma and cortex of the kidney were greatly enhanced after injection and the peak time could last more than 50 ***: This new lipid ultrasound contrast agent could significantly enhance the grey scale imaging of the hepatic parenchyma and the renal cortex for more than 50 min.

关键词：灰白刻度野兔肝脏肾脏静脉内注射脂质涂层超声波诊断

Epidemiology of peptic ulcer disease in Wuhan area of China from 1997 to 2002

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World Journal of Gastroenterology 2004年第22期10卷 3377-3379页

作者： Wei-GuoDong Chun-ShengCheng Shao-pingliu Jie-PingYu DepartmentofGastroenterology RenminHospitalofWuhanUniversityWuhan430060HubeiProvinceChina DepartmentofGastroenterology HuangshiCentralHospitalHuangshi435000HubeiProvinceChina

AIM: To describe the eplclemlologlcal Teatures or peptic ulcer disease in Wuhan area during 1997-2002, to analyze the sex, age and occupation characteristics, as well as the geographic distribution of peptic ulcer disease, and to determine the effective methods of preventing and controlling peptic ulcer disease. METHODS: In the early 1980s, the peptic ulcer disease registry system was established to collect the data of peptic ulcer disease in Wuhan area. Here we performed a statistically detailed analysis of 4876 cases of peptic ulcer disease during 1997-2002. RESULTS: The morbidity of peptic ulcer disease between males and females was significantly different (x^2=337.9, P<0.001). The majority of peptic ulcer diseases were found at the age of 20 to 50 years. Because of different occupations, the incidence of peptic ulcer disease was different in different areas. CONCLUSION: The incidence of peptic ulcer disease is highly associated with sex, age, occupation and geographic environmental factors. By analyzing the epidemiological features of peptic ulcer disease, we can provide the scientific data for prevention and control of peptic ulcer disease.

关键词：流行病学消化性溃疡消化系统疾病武汉中国 1997年 2002年预防措施

A Survey and Statistics of Interstellar OH and H_2O Masers

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Chinese Journal of Astronomy and Astrophysics 2005年第2期5卷 175-183页

作者： Han-pingliu J.R.Forster JinSun DepartmentofPhysics BeijingNormalUniversityBeijing100875 UniversityofCalifornia BerkeleyandHatCreekRadioObservatory42231BidwellRoadHatCreekCA96040 DepartmentofAstronomy BeijingNormalUniversityBeijing100875

We present a statistical analysis of a sky survey of interstellar H2O and OH masers. These masers can be classified into three categories: isolated H2O masers, isolated OH masers, and simple OH/H2O maser associations. The total number of sources in each category is of the same order of magnitude, and as an evolutionary phase they can maintain ~ 105 yr. An improved radiative pumping mechanism is proposed. This model avoids some of the deficiencies of previous radiative models, such as shortage of exciting photons. The statistical results obtained from the survey can be interpreted by the new mechanism together with the evolutionary model in which the gravitational force of the central stellar objects is responsible for the HII region.

关键词： surveys masers radiation mechanism: non-thermal HII regions

Multicenter clinical study on Fuzhenghuayu capsule against liver fibrosis due to chronic hepatitis B

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World Journal of Gastroenterology 2005年第19期11卷 2892-2899页

作者： pingliu Yi-YangHu ChengLiu Lie-MingXu Cheng-HaiLiu Ke-WeiSun De-ChangHu You-KuanYin xia-QiuZhou Mo-BinWan XiongCai Zhi-QingZhang JUNYe Ren-XingZhou JiaHe Bao-ZhangTang InstituteofLiverDisease ShanghaiUniversityofTraditionalChineseMedicineShanghai201203China ShanghaiSecondMilitaryMedicalUniversity Shanghai200233China theAffiliatedHospital YunnanCollegeofTCMYunnan650000KunmingProvinceChina theAffiliatedHospital HunanCollegeofTCMChangsha410000HunanProvinceChina ZhongshanHospital FudanUniversityShanghai200032.China RuijinHospital ShanghaiSecondMedicalUniversityShanghai200025China ChanghaiHospital Shanghai200423China ShanghaiChangzhengHospital Shanghai200236China the4thPeopleHospitalofHuaianCity Huaian232000JiangsuProvinceChina theCentralHospitalofPutuoDistrict Shanghai200062China ShanghaiModemTechniqueCo. LtdforTCMShanghai200037China

AIM: To study the efficacy and safety of Fuzhenghuayu capsule (FZHY capsule, a capsule for strengthening body resistance to remove blood stasis) against liver fibrosis due to chronic hepatitis B. METHODS: Multicenter, randomized, double blinded and parallel control experiment was conducted in patients (aged from 18 to 65 years) with liver fibrosis due to chronic hepatitis B. Hepatic histologic changes and HBV markers were examined at wk 0 and 24 during treatment. Serologic parameters (HA, LM, P-Ⅲ-P, Ⅳ-C) were determined and B ultrasound examination of the spleen and liver was performed at wk 0, 12 and 24. Liver function (liver function and serologic parameters for liver fibrosis) was observedat wk 0, 6, 12, 18 and 24. Blood and urine routine test, renal function and ECG were examined before and after treatment. RESULTS: There was no significant difference between experimental group (110 cases) and control group (106 cases) in demographic features, vital signs, course of illness, history for drug anaphylaxis and previous therapy, liver function, serologic parameters for liver fibrosis, liver histologic examination (99 cases in experimental group, 96 cases in control group), HBV markers, and renal function. According to the criteria for liver fibrosis staging, meanscore of fibrotic stage(s) in experimental group after treatment (1.80) decreased significantly compared to the previous treatment (2.33, P＜0.05), but there was no significant difference in mean score of fibrotic stage(s) (2.11 and 2.14 respectively). There was a significant difference in reverse rate between experimental group (52%) and control group (23.3%) in liver biopsy. With marked effect on decreasing the mean value of inflammatory activity and score of inflammation (P＜0.05), Fuzhenghuayu capsule had rather good effects on inhibiting inflammatory activity and was superior to that of Heluoshugan capsule. Compared to that of pretreatment, there was a significant decrease in HA, LM, P-Ⅲ-P and Ⅳ-C content

关键词： Chronic hepatitis B Fuzhenghuayu capsule

Signaling on Telomerase:A Master Switch of Cellular Immortalization in Tumor Cells

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Signaling on Telomerase:A Master Switch of Cellular Immortal...

Protective effect of angelica sinensis polysaccharide on experimental immunological colon injury in rats

Asian-Pacific Conference of Tumor Biology 2001

作者： HeLi YingCao SiddharthaDeb SuadHadzic Jun-pingliu Baker Medical Research Institute MelbourneAustralia Baker Medical Research Institute MelbourneAustralia Baker Medical Research Institute MelbourneAustralia Baker Medical Research Institute MelbourneAustralia Baker Medical Research Institute MelbourneAustralia

＜正＞Telomerase is a ribonucleoprotein complex Gontaining telomerase reverse transcriptase （TERT） respon-sible for the maintenance and elongation of telomeres （the ends of chromosomes）. Considerable evidencesuggests cells during tumorigenesis. Telomerase is activated in most cancers and activaion occurs withincreased gene expression of TERT and protein phosphorylation and heat shock proein -dependent as-sembly of telomerase holoenzymp. Forced ectopic expression of TERT in normal fibroblats induces con-tinuous cell proliferation toward immortalianion. To explore the control mechanisms as well as cellular ac-tions of telomerase, we found that telomerase can be regulated by mukiple distinct mechanisms including

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World Journal of Gastroenterology 2003年第12期9卷 2786-2790页

作者： Shao-pingliu Wei-GuoDong Dong-FangWu He-ShengLuo Jie-PingYu DepartmentofGastroenterology RenminHospitalofWuhanUniversity238JiefangRoadWuhan430060HubeiProvinceChina DepartmentofPharmacy RenminHospitalofWuhanUniversity238JiefangRoadWuhan430060HubeiProvinceChina

AIM: To study the effect of angelica sinensis polysaccharide (ASP) on immunological colon injury and its mechanisms in ***: Immunological colitis model of rats was induced by intracolon enema with 2, 4, 6-trinitrobenzene sulfonic acid (TNBS) and ethanol. The experimental animals were randomly divided into normal control, model control, 5-aminosalicylic acid therapy groups and three doses of ASP therapy groups. The 6 groups were treated intracolonically with normal saline, normal saline, 5-aminosalicylic acid (100 ***-1), and ASP daily (8:00 am) at the doses of 200, 400 and 800 ***-1 respectively for 21 days 7 d following induction of colitis. The rat colon mucosa damage index (CMDI), the histopathological score (HS), the score of occult blood test (OBT), and the colonic MPO activity were evaluated. The levels of SOD, MDA, NO, TNF-α, IL-2 and IL-10 in colonic tissues were detected biochemically and immunoradiometrically. The expressions of TGF-β and EGF in colonic tissues were also determined ***: Enhanced colonic mucosal injury, inflammatory response and oxidative stress were observed in colitis rats,which manifested as significant increases of CMDI, HS, OBT,MPO activity, MDA and NO contents, as well as the levels of TNF-α and IL-2 in colonic tissues, although colonic TGF-β protein expression, SOD activity and TL-10 content were significantly decreased compared with the normal control (P＜0.01). However, these parameters were found to be significantly ameliorated in colitis rats treated intracolicly with ASP at the doses of 400 and 800 mg@kg-1 (P＜0.05-0.01).Meantime, colonic EGF protein expression in colitis rats was remarkably ***: ASP has a protective effect on immunological colon injury induced by TNBS and ethanol enema in rats,which was propably due to the mechanism of antioxidation,immunomodulation and promotion of wound repair.

关键词：当归多糖免疫损伤药理作用动物实验