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Idiopathic sclerosing encapsulating peritonitis (or abdominal cocoon):A report of 5 cases

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World Journal of Gastroenterology 2007年第26期13卷 3649-3651页

作者： ping Xu Li-hua chen You-Ming Li Department of Gastroenterology First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 Zhejiang Province China

Sclerosing encapsulating peritonitis (SEP) is a rare cause of intestinal obstruction that is characterized by a thick grayish-white fibrotic membrane encasing the small bowel. SEP can be classified as idiopathic,also known as abdominal cocoon,or secondary. It is difficult to make a definite pre-operative diagnosis. We experienced five cases of abdominal cocoon,and the case files were reviewed retrospectively for the clinical presentation,operative findings and outcome. All the patients presented with acute,subacute and chronic intestinal obstruction. Computed tomography (CT) showed characteristic findings of small bowel loops congregated to the center of the abdomen encased by a soft-tissue density mantle in four cases. Four cases had an uneventful post-operative period,one case received second adhesiolysis due to persistent ileus. The imaging techniques may facilitate pre-operative diagnosis. Surgery is important in the management of SEP.

关键词： Sclerosing encapsulating peritonitis Idiopathic Abdominal cocoon Intestinal obstruction Adhesiolysis

High-throughput Procedure for Single Pollen Grain Collection and Polymerase Chain Reaction in Plants

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Journal of Integrative Plant Biology 2008年第3期50卷 375-383页

作者： ping-hua chen Yong-Bao Pan Ru-Kai Che USDA-ARS Southem Regional Research Center Sugarcane Research Laboratory Houma LA 70360 USA Key Laboratory of Eco-physiology & Genetic Improvement for Sugarcane Ministry of Agriculture Fujian Agriculture and Forestry University Fujian Normal University Fuzhou 350002 China

Single pollen grain polymerase chain reaction （PCR） has succeeded in several species, however only limited numbers of pollen grains were involved due to difficulties in pollen isolation and lysis. This has limited its application in genetic analysis and mapping studies in plants. A high-throughput （HT） procedure for collecting and detecting genetic variation in a large number of individual pollen grains by PCR is reported. The HT procedure involved the collection of individual pollen grains by a pair of special forceps and the lysis of pollen grains in a heated alkali/detergent solution followed by neutralization with a tris-ethylenediamine tetraacetic acid （TE） buffer. These resulting template solutions yielded PCR reactions involving the 5S ribosomal RNA intergenic spacers, randomly amplified polymorphic DNA, and simple sequence repeats markers. Using this procedure, one person with experience could collect and process up to 288 single pollen grain PCR reactions per day. The method worked well on sugarcane, corn, Miscanthus spp., snap bean, sorghum, and tomato. The ability to collect and conduct PCR on individual pollen grains on a large scale offers a new approach to genetic analyses and mapping studies in an easily controllable environment with a considerable cost reduction. The method will also significantly benefit studies in species that are difficult subjects for classical genetic research.

关键词： DNA marker high-throughput polymerase chain reaction single pollen grain

Extra-pulmonary tuberculosis infection in the dialysis patients with end stage renal diseases:case reports and literature review

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第1期14卷 76-82页

作者： Wen-fang YANG Fei HAN Xiao-hui ZHANG ping ZHANG Jiang-hua chen Kidney Disease Center the First Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310003 China

The diagnosis of extra-pulmonary tuberculosis(TB) seems relatively difficult due to the absence of specific symptoms and signs in patients on peritoneal dialysis or *** report four cases of extra-pulmonary tuberculosis on dialysis,with two cases on peritoneal dialysis and two cases on *** presentations,therapy,and outcomes of TB infection in these patients were ***,the English literature published in the PubMed database associating extra-pulmonary tuberculosis on dialysis over the last three decades is reviewed.A total of 61 studies containing 70 cases were *** most common primary disease was diabetic nephropathy(22.86%,16/70).The peritoneum(31.42%,22/70),bone(21.42%,15/70),and lymph node(20%,14/70) were the most frequently *** organ infection was common(90%,63/70).Fever(58.57%,41/70),pain(35.71%,25/70),and enlarged lymph node(20%,14/70) were the most common ***(67.14%,47/70) and culture(40%,28/70) provided most reliable methods for clear diagnosis of *** combined treatment of isoniazid,rifampicin,pyrazinamide,and ethambutol(44.29%,31/70) was the most common *** majority of patients improved(82.86%,58/70);however,12 cases got worse(17.14%),with 10 of them dying(14.29%).Physicians should be aware of the non-specific symptoms and location of infection,and consider tuberculosis in their differential diagnoses in dialysis patients presenting with symptoms such as fever,pain,and weight loss.

关键词： Extra-pulmonary tuberculosis End stage renal disease Dialysis

Immune algorithm for discretization of decision systems in rough set theory

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2006年第4期7卷 602-606页

作者： JIA ping DAI Jian-hua chen Wei-dong PAN Yun-he ZHU Miao-liang Institute of Artificial Intelligence Zhejiang University Hangzhou 310027 China

Rough set theory plays an important role in knowledge discovery, but cannot deal with continuous attributes, thus discretization is a problem which we cannot neglect. And discretization of decision systems in rough set theory has some particular characteristics. Consistency must be satisfied and cuts for discretization is expected to be as small as possible. Consistent and minimal discretization problem is NP-complete. In this paper, an immune algorithm for the problem is proposed. The correctness and effectiveness were shown in experiments. The discretization method presented in this paper can also be used as a data pre- treating step for other symbolic knowledge discovery or machine learning methods other than rough set theory.

关键词： Rough sets Discretization Immune algorithm Decision system

Quantum Neural Network for Quantum Neural Computing

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Research 2024年第1期2023卷 71-80页

作者： Min-Gang Zhou Zhi-ping Liu hua-Lei Yin chen-Long Li Tong-Kai Xu Zeng-Bing chen National Laboratory of Solid State Microstructures School of PhysicsCollaborative Innovation Center of Advanced MicrostructuresNanjing UniversityNanjing 210093China MatricTime Digital Technology Co.Ltd. Nanjing 211899China

Neural networks have achieved impressive breakthroughs in both industry and *** to effectively develop neural networks on quantum computing devices is a challenging open ***,we propose a new quantum neural network model for quantum neural computing using(classically controlled)single-qubit operations and measurements on real-world quantum systems with naturally occurring environment-induced decoherence,which greatly reduces the difficulties of physical *** model circumvents the problem that the state-space size grows exponentially with the number of neurons,thereby greatly reducing memory requirements and allowing for fast optimization with traditional optimization *** benchmark our model for handwritten digit recognition and other nonlinear classification *** results show that our model has an amazing nonlinear classification ability and robustness to ***,our model allows quantum computing to be applied in a wider context and inspires the earlier development of a quantum neural computer than standard quantum computers.

关键词： quantum thereby breakthrough

Effect of Small Needle Knife on Autophagy and TGF-β in Synovial Fluid of KOA Rats

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Chinese Journal of Biomedical Engineering(English Edition) 2024年第1期33卷 33-39页

作者： ZHENG Kang-hua LIN Zeng-ping chen Yong Fujian Provincial 2nd People's Hospital Affiliated Hospital of Fujian University of Traditional Chinese MedicineFuzhou Fujian 350003China College of Integrative Chinese and Western Medicine Fujian University of Traditional Chinese MedicineFuzhou Fujian 350122China

Objective:To explore the effects of small needle knife on the autophagy function of synovium and the expression of TGF-βin synovial fluid of KOA rats,so as to provide a new theoretical basis for the treatment of ***:48 SD rats were randomly divided into blank control group,model group,drug group and acupotomy *** blank control group maintained the original feeding without any intervention,the model group adopted the sodium monoiodoacetate injection method to prepare the KOA rat model without any intervention,the drug group prepared the KOA rat model and then treated with tripterine by gavage,and the needle knife group treated the KOA rat model with small needle *** expression levels of autophagy-related proteins in synovial tissues were detected by immunohistochemistry and Western blotting,and the content of TGF-βin synovial fluid was measured by ***:Compared with the blank control group,Beclin-1,Caspase-3 and Bax were up-regulated and m TOR was down-regulated in the model ***-1,m TOR,Caspase-3 and Bax were up-regulated in the drug *** was no significant difference in the expression of autophagy-related proteins Beclin-1(0.28±0.09)and m TOR(0.45±0.12)between the acupotomy group and the blank control group,while Caspase-3(0.17±0.03)and Bax(0.30±0.01)were *** expression level of TGF-βin synovial fluid in the small needle knife group was(0.29±0.09),which was similar to that in the blank control group,and was significantly higher than that in the model ***:Small needle knife treatment can promote the recovery of synovial autophagy in KOA rats,and reduce the level of TGF-βin synovial fluid,and can play a clinical role in the treatment of KOA by regulating the expression of synovial autophagy and TGF-β.

关键词： small needle knife knee osteoarthritis(KOA) synovium autophagy transforming growth factorβ(TGF-β)

Goal-Oriented Control Systems(GOCS):From HOW to WHAT

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IEEE/CAA Journal of Automatica Sinica 2024年第4期11卷 816-819页

作者： Wen-hua chen the Department of Aeronautical and Automotive Engineering Loughborough UniversityLeicestershireLE113TUU.K.

Brief:New control theory is required to underpin safe design and deployment of future highly automated systems to deal with uncertain environments and complicated tasks,enabled by AI and other advanced ***-Oriented Control Systems offer potential to transform the control system design from currently instructing a control system how to perform a task to specifying what is to be achieved.

关键词： automated instru system

Identification and Detection of Actinobacillus pleuropneumoniae in Infected and Subclinically Infected Pigs by Multiplex PCR Based on the Genes ApxIVA and OmlA

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Agricultural Sciences in China 2006年第2期5卷 146-154页

作者： XIAO Guo-sheng CAO San-jie DUAN Li-li WEN Xin-tian MA Xiao-ping chen hua-mei Laboratory of Animal Infectious Disease and Microarray/Key Laboratory of Animal Disease and Human Health of Sichuan Province College of Animal Science and Veterinary Medicine Sichuan Agricultural University Ya＇an 625014 P.R.China

PCRs based on different genes of Actinobacillus pleuropneumoniae have been developed for detecting and identifying A. pleuropneumoniae. Some of them could amplify positive fragments from the phylogenetically closely related species bacteria. To improve veracity and specificity of PCR, a species-specific multiplex PCR assay was developed to identify and detect A. pleuropneumoniae, based on the 3＇-terminus of the species-specific apxlVA gene and the already existing species-specific primers in the omlA gene. Both 346-bp and 950-bp fragments could be simultaneously amplified from all A. pleuropneumoniae reference strains and isolates, and the species specificity of the assay was evaluated with a collection of ten strains representing eight different species bacteria including species normally found in the respiratory tracts of swine. All of these strains turned out negative in the multiplex PCR. All sequences of products of multiplex PCR randomly sampled were also correct. The sensitivity of the multiplex PCR was determined to be 10 pg of A. pleuropneumoniae DNA. The multiplex PCR and bacterial isolation were compared to determine their sensitivities by using experimentally infected pigs and clinical disease pigs. The multiplex PCR was more sensitive than bacterial isolation. The multiplex PCR was also evaluated on mixed bacterial cultures from clinical healthy pigs. 26/100 （26%） of the subclinically infected pigs were detected from clinical healthy pigs. The results indicate that the multiplex PCR assay is a sensitive, highly specific, and effective diagnostic tool for identification and detection of A. pleuropneumoniae.

关键词： multiplex PCR Actinobacillus pleuropneumoniae pig bacteria apxIVA and omlA genes

Manipulating submolecular-scale phototautomerization

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Science China Materials 2024年第6期67卷 2048-2050页

作者： ping Zhou Hongliang chen Stoddart Institute of Molecular Science Department of ChemistryZhejiang UniversityHangzhou 310058China Zhejiang-Israel Joint Laboratory of Self-Assembling Functional Materials ZJU-Hangzhou Global Scientific and Technological Innovation CenterZhejiang UniversityHangzhou 311215China

A recent study on the submolecular-scale control of phototautomerization methods and the underlying reaction mechanisms was reported in Nature Nanotechnology by Guillaume and co-workers[1],elucidating how the field confinement effect generated by the scanning tunneling microscope(STM)tip precisely triggers the phototautomerization of a free-base phthalocyanine at the submolecular level and demonstrating that the reaction process can be finely controlled by adjusting either the laser excitation wavelength or the position of the tip.

关键词： tunneling excitation phthalocyanine