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Acta Pharmacologica Sinica 2007年第7期28卷 1031-1036页

作者： Qiu-bai LI Zhi-chao CHEN Yong YOU ping zou Department of Hematology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China Department of Hematology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China

Aim:To investigate the effects of small interfering RNA of cyclooxygenase-2(COX-2) on the proliferation and apoptosis of human multiple myeloma RPMI8226cells and its relation with the Bcl-2 family in ***:Transcription andexpression of COX-2 in human myeloma RPMI8226 cells were checked by RT-PCRand Western blot analysis,*** COX-2 siRNA fragment targetingexon 5 of COX-2 gene was transfected into the cells with the Amaxa *** inhibition of cell growth was detected by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium (MTT) *** was estimatedby Annexin-V/propidium iodide double-labeled cytometry and confirmed by termi-nal deoxynucleotidyl transferase-mediated dUTP nick end-labeling ***-2and Bax expression was evaluated by Western blot ***:The COX-2siRNA fragment could be successfully transfected into RPMI8226 cells,whichresulted in the significant inhibition of transcription and expression of COX-2 inthe myeloma *** of the transfected cells was inhibited andapoptosis was induced (6.52%±0.32%,12.53%±2.52%,24.39%±3.51% and36.48%±4.96% for 0,12,24,and 48 h,respectively) in a time-dependent manner(P<0.01).However,the expression of Bcl-2 and Bax in the RPMI8226 cells had nosignificant changes after ***:COX-2 siRNA transfectioncan suppress COX-2 expression in human myeloma RPMI8226 cells,which leadsto growth inhibition and apoptosis independent of Bcl-2.

关键词： RPMI8226 small interfering RNA apoptosis proliferation

Inhibition of Fas-mediated Apoptosis in Yac-1 Cell via Anti-Fas Ribozyme

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Acta Biochimica et Biophysica Sinica 2004年第3期36卷 199-205页

作者： Min ZHANG, Fang LIU, Wei HE, Yong YOU, ping zou*, and Ling-Bo LIU Institute of Hematology Disease, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China Institute of Hematology Disease Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China

To detect a new and more effective way against apoptosis mouse lymphomatic cell line- Yac-1 in which fas gene was expressed highly was used as a model for studying the effects of anti-Fas ribozyme on Fas-mediated apoptosis. A hammerhead ribozyme gene targeting the fas mRNA was synthesized and its in vitro transcription vector was constructed, which was transfected into Yac-1 cells using electroporation. Rz596 expression was detected using RT-PCR, and Fas expression in Yac-1 cells was detected using RT-PCR, Western blot and flow cytometry. After treated with anti-Fas antibody (JO2), Yac-1 cell viability was measured with MTT assay, caspase-3 proteolytic activity was detected, and cell apoptosis was measured according to annexin V apoptosis detecting kit. Anti-Fas ribozyme could cleave fas mRNA efficiently in vivo and in vitro. Fas expression in Yac-1 cells transfected with anti-Fas ribozyme was decreased remarkably and correlated with resistance to Fas-mediated apoptosis as determined by flow cytometry and caspase-3 proteolytic activity. Anti-Fas ribozyme was detected in cells transfected with pU6-RZ596 and pU6-dRZ596 and could remarkably decrease the Fas expression in Yac-1 cells, which made Yac-1 cells get rid of Fas-mediated apoptosis. Because of wide expression of fas in organs and tissues, our research was very useful for studying the inhibition of apoptosis of many organs and tissues in the future.

关键词： transplantation immunology ribozyme Fas Yac-1 apoptosis

Quinoxaline-based Polymers with Asymmetric Aromatic Side Chain Enables 16.27% Efficiency for Organic Solar Cells

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Chinese Journal of Polymer Science 2023年第7期41卷 1002-1010页

作者： Dong-Xu Li Shu-Fang Li Cheng-Long Wen Chao-Yuan Sun Chang-Zhou Shi Xin-Xin Xia Xin-Hui Lu Li-Hui Jiang Jun Yuan Ying-ping zou College of Chemistry and Chemical Engineering Central South UniversityChangsha410083China Department of Physics The Chinese University of Hong KongNew TerritoriesHong Kong999077China

In recent years, due to the rapid development of high-performance small molecule acceptor (SMA) materials, the researches on p-type electron donor materials for matching with current efficient SMAs have become important. By means of asymmetric strategies to optimize the energy levels and inter/intramolecular interactions of molecules, we designed and synthesized an asymmetric aromatic side chain quinoxaline-based polymer donor TPQ-0F. Meanwhile, we took advantage of F atom which could form noncovalent interaction and strong electron-withdrawing property, to obtain the optimized quinoxaline-based polymer donors TPQ-1F, TPQ-1Fi and TPQ-2F. Eventually, the binary device based on TPQ-2F achieved an efficient power conversion efficiency (PCE) of 16.27%, which attributed to balanced hole/electron mobilities, less charge carrier recombination, and more favorable aggregation morphology. Our work demonstrates the great potential of asymmetric aromatic side chain quinoxaline-based polymer donors on optimizing the morphology of blending films, improving inter/intramolecular interactions, and subtly tuning energy level, finally for more efficient organic solar cells.

关键词： Asymmetric aromatic side chain Quinoxaline-based polymer donor Fluorine substitution Binary device

Sirolimus can promote the disappearance of renal angiomyolipoma associated with tuberous sclerosis complex:a prospective cohort study

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World Journal of Pediatrics 2024年第6期20卷 602-610页

作者： Shuo Dun Yang-Yang Wang Lin Wan Qiu-Hong Wang Qian Lu Xiao-Yan Yang Qi Zhang Hui-Min Chen Lu-Peng Qiu Li-ping zou Medical School of Chinese PLA Beijing 100853China Department of Pediatrics The First Medical CenterChinese PLA General HospitalBeijing 100853China Center of Epilepsy Beijing Institute for Brain DisordersBeijing 100069China

Background Renal angiomyolipoma(RAML)is the most common kidney lesion in patients with tuberous sclerosis complex(TSC),affecting about 80%of *** is a benign tumor that grows over time,usually bilaterally,and can easily lead to kidney complications such as acute ***,we investigated the efficacy and safety of sirolimus in children with TSC-associated RAML and explored the factors affecting tumor disappearance under sirolimus treatment through subgroup *** A prospective cohort study was *** was initiated at 1 mg/(m^(2)×day),and dose adjustments were made by a 2-week titration period to attain a trough blood concentration of 5-10 ng/*** disappearance of RAML in chil-dren after sirolimus treatment was observed,and Cox regression was used to screen the factors affecting tumor *** One hundred and twenty-six patients who met the criteria were *** 3 months,6 months,12 months,and 24 months of follow-up,tumors disappeared in 18(14.3%),30(23.8%),39(31.0%),and 42(33.3%)children,*** disappeared in 50(39.7%)children by the last visit of each individual,and 30(60%)of them occurred within 6 *** multivariate Cox regression analysis showed that patients with a smaller maximum tumor diameter at baseline had a higher tumor disappearance ***-six(29%)patients had stomatitis during the entire treatment period,and no serious *** Sirolimus could promote the disappearance of TSC-related *** disappearance rate was correlated with the maximum diameter at baseline,and the smaller the tumor was,the higher the disappearance *** is well tolerated in the treatment of RAML associated with TSC.

关键词： Angiomyolipoma mTOR inhibitor Sirolimus Tuberous sclerosis

Proteomic analysis of the immune response of the silkworm infected by Escherichia coli and Bacillus bombyseptieus

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Insect Science 2012年第5期19卷 559-569页

作者： Xiao-Wu Zhong ping Zhao Yong zou Hong-Yi Nie Qi-Ying Yi Qing-You Xia Zhong-Huai Xiang The Key Sericultural Laboratory of Agriculture Ministry Southwest University Chongqing China

he silkworm, Bombyx mori, is an economically important insect with a 5 000-year history of domestication. During evolution, the silkworm has developed highly effective defenses against invasion and parasitization by microorganisms. In this study, two microorganisms Escherichia coli and Bacillus bombyseptieus were orally infected to silk- worm larvae. After infection with E. coli and B. bombyseptieus for 24 h, we investigated the polypeptide changes in the hemolymph, midgut and integument using two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time of flight mass spec- trometry. Forty-seven differentially expressed proteins were identified in these tissues. They belonged to a variety of functional classes, including immune proteins, metabolic proteins and structural proteins. Compared with controls, E. coli-infected silkworms showed 21 up- regulated proteins, 25 down-regulated proteins and lost one protein. After infection with B. bombyseptieus, silkworms showed 15 up-regulated proteins, 27 down-regulated pro- teins, lost three proteins and retained two proteins unchanged. We speculate that all these proteins may play a role in the silkworm immune response, although it is unclear why and how the two kinds of bacteria can so markedly alter expression of these proteins. These re- sults offer valuable insights for measuring the proteomic responses of the silkworm innate immune mechanism.

关键词： Bacillus bombyseptieus Escherichia coli immune response proteomics silkworm

历史记忆的转换与身份认同——论《萨拉米斯士兵》的叙事策略

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当代外国文学 2018年第1期39卷 47-54页

作者：邹萍南京师范大学外国语学院

哈维尔·塞尔卡斯的《萨拉米斯士兵》是当代西班牙历史回忆小说创作的里程碑式作品。小说以多声部叙述、空间转换的记忆激活等叙事策略,在讲述西班牙内战期间真实故事的同时,又展示了作品从构思到完成的文学创作全程,为读者揭示了叙事... 详细信息

哈维尔·塞尔卡斯的《萨拉米斯士兵》是当代西班牙历史回忆小说创作的里程碑式作品。小说以多声部叙述、空间转换的记忆激活等叙事策略,在讲述西班牙内战期间真实故事的同时,又展示了作品从构思到完成的文学创作全程,为读者揭示了叙事的艺术建构,因此又被认为是元小说。该小说创作的独特艺术风格,不仅为历史题材小说如何把历史的真实性与文学的艺术性融合起来,提供了值得借鉴的新路径,更深刻表现了西班牙知识分子的民族认同感和作家文学创作的艺术追求。

关键词：哈维尔·塞尔卡斯《萨拉米斯士兵》西班牙文学叙事策略

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云南教育供给侧结构性改革的若干思考

教育研究 2016年第11期37卷 150-155页

作者：邹平云南省教育厅

教育供给与需求的矛盾是客观存在的,因此有必要研究教育供给侧结构性改革。推进教育供给侧结构性改革是教育持续发展的重要途径,要坚持问题为导向,改革完善教育评价制度,以科学方式增加教育的有效供给,确保合理的教育供给结构,提高教育... 详细信息

教育供给与需求的矛盾是客观存在的,因此有必要研究教育供给侧结构性改革。推进教育供给侧结构性改革是教育持续发展的重要途径,要坚持问题为导向,改革完善教育评价制度,以科学方式增加教育的有效供给,确保合理的教育供给结构,提高教育供给质量,改善供给方式和供给环境。

关键词：供给侧结构性改革有效供给供给结构供给质量供给环境

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货币政策、股票流动性与资本结构动态调整

审计与经济研究 2015年第1期30卷 74-82页

作者：邹萍中南财经政法大学会计学院湖北武汉430073

以2003—2012年沪深A股上市公司为研究样本,考察货币政策和股票流动性对资本结构动态调整的影响。研究结果表明:伴随着股票流动性的上升,资本结构调整速度显著加快;货币政策的放松有利于资本结构调整速度的改善,而且股票流动性与资本结... 详细信息

以2003—2012年沪深A股上市公司为研究样本,考察货币政策和股票流动性对资本结构动态调整的影响。研究结果表明:伴随着股票流动性的上升,资本结构调整速度显著加快;货币政策的放松有利于资本结构调整速度的改善,而且股票流动性与资本结构调整速度的关系也在宽松的货币政策下变得更加敏感。进一步研究发现,在不同的产权性质和负债水平下,货币政策、股票流动性对资本结构动态调整的影响存在非对称性:在非国有性质和负债较低的上市公司中影响更显著,而在国有性质和负债较高的上市公司中影响更弱。

关键词：货币政策股票流动性资本结构动态调整产权性质负债水平微观公司行为最优资本结构公司资本结构调整

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地区经济发展、社会责任行为与科技创新投入

科学学研究 2018年第5期36卷 922-932页

作者：邹萍华中农业大学经济管理学院

目前许多研究表明社会责任和科技创新都有助于企业价值的增长，然而多数研究仅将这二者孤立的进行探讨，实践中许多企业既进行了科技创新投入又有社会责任行为，本文将这两种行为置于一个框架下探讨二者对于企业价值的交互效应。以中国2... 详细信息

目前许多研究表明社会责任和科技创新都有助于企业价值的增长，然而多数研究仅将这二者孤立的进行探讨，实践中许多企业既进行了科技创新投入又有社会责任行为，本文将这两种行为置于一个框架下探讨二者对于企业价值的交互效应。以中国2007-2014年A股上市公司为样本，检验了企业社会责任行为与科技创新投入对企业价值的交互效应，并考察了地区经济发展水平对该交互效应的影响，研究结果表明：社会责任行为与科技创新投入对企业价值的交互效应为替代效应，即社会责任行为与科技创新投入共存一定程度上削弱了对企业价值增长的促进作用；动态研究的结果同样表明，社会责任行为与科技创新投入对企业价值的影响存在替代效应；地区经济发展水平是该替代效应的外部激发机制，在经济发展水平较高的地区，存在显著的替代效应，而在经济发展水平较低地区不显著。进一步研究发现，成长性高的企业替代效应更显著，同时地区经济发展水平对该替代效应的影响也仅在成长性高的企业中存在。本研究反映了社会责任与科技创新对于企业价值的交互效应以及这种效应的外部激发机制，打开了社会责任与科技创新促进企业价值增长作用的黑箱。

关键词：社会责任行为科技创新投入替代效应地区经济发展企业价值

Ruxolitinib add-on in corticosteroid-refractory graft-vs-host disease after allogeneic stem cell transplantation:Results from a retrospective study on 38 Chinese patients

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World Journal of Clinical Cases 2020年第6期8卷 1065-1073页

作者： Si-Hua Dang Qin Liu Rong Xie Na Shen Shu Zhou Wei Shi Wen Liu ping zou Yong You Zhao-Dong Zhong Department of Hematology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030Hubei ProvinceChina Department of Oncology Luohe Central HospitalLuohe 462300Henan ProvinceChina Department of Gynecology and Obstetrics Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030Hubei ProvinceChina

BACKGROUND Graft-vs-host disease (GVHD) is a major cause of mortality after allogeneic hematopoietic stem cell *** patients have steroid-refractory(SR) *** To evaluate the effect and safety of ruxolitinib add-on in the treatment of patients with SR acute (a) and chronic (c) *** We retrospectively analyzed 38 patients administered ruxolitinib add-on to standard immunosuppressive therapy for SR-aGVHD or SR-cGVHD following allogeneic hematopoietic stem cell *** was administered5-10 mg/d depending on disease severity,patient status,and the use of antifungal *** response rate,time to best response,malignancy relapse rate,infection rate,and treatment-related adverse events were *** The analysis included 10 patients with SR-aGVHD (gradeⅢ/Ⅳ,n=9) and 28patients with SR-cGVHD (moderate/severe,n=24).For the SR-aGVHD and SRcGVHD groups,respectively:Median number of previous GVHD therapies was 2(range:1-3) and 2 (1-4);median follow-up was 2.5 (1.5-4) and 5 (1.5-10) mo;median time to best response was 1 (0.5-2.5) and 3 (1-9.5) mo;and overall response rate was 100%(complete response:80%) and 82.1%(complete response:10.7%) with a response observed in all GVHD-affected *** malignancy relapse rates for the SR-aGVHD and SR-cGVHD groups were 10.0%and 10.7%,*** rates for cytomegalovirus,Epstein-Barr virus,and varicella-zoster virus,respectively,were 30.0%,10.0%,and 0%for the SR-aGVHD group and 0%,14.3%,and 7.1%for the SR-cGVHD *** Ruxolitinib add-on was effective and safe as salvage therapy for SR-GVHD.

关键词： Graft-vs-host disease Graft-vs-leukemia effect Allogeneic hematopoietic stem cell transplantation Ruxolitinib Treatment Antifungal drugs