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Mass spectrometry analysis of intact protein N-glycosylation signatures of cells and sera in pancreatic adenocarcinomas

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2024年第1期25卷 51-64页

作者： Mingming XU Zhaoliang LIU Wenhua HU Ying HAN Zhen WU Sufeng CHEN Peng XIA Jing DU Xumin ZHANG piliang hao Jun XIA Shuang YANG Center for Clinical Mass Spectrometry College of Pharmaceutical SciencesSoochow UniversitySuzhou215123China School of Life Science and Technology ShanghaiTech UniversityShanghai201210China State Key Laboratory of Genetic Engineering Department of BiochemistrySchool of Life SciencesFudan UniversityShanghai200438China Department of Clinical Laboratory Center Zhejiang Provincial People’s HospitalPeople’s Hospital of Hangzhou Medical CollegeHangzhou310014China

Pancreatic cancer is among the most malignant cancers,and thus early intervention is the key to better survival ***,no methods have been derived that can reliably identify early precursors of development into ***,it is urgent to discover early molecular changes during pancreatic *** aberrant glycosylation is closely associated with cancer progression,numerous efforts have been made to mine glycosylation changes as biomarkers for diagnosis;however,detailed glycoproteomic information,especially site-specific N-glycosylation changes in pancreatic cancer with and without drug treatment,needs to be further ***,we used comprehensive solid-phase chemoenzymatic glycoproteomics to analyze glycans,glycosites,and intact glycopeptides in pancreatic cancer cells and patient *** profiling of N-glycans in cancer cells revealed an increase in the secreted glycoproteins from the primary tumor of MIA PaCa-2 cells,whereas human sera,which contain many secreted glycoproteins,had significant changes of glycans at their specific *** results indicated the potential role for tumor-specific glycosylation as disease *** also found that AMG-510,a small molecule inhibitor against Kirsten rat sarcoma viral oncogene homolog(KRAS)G12C mutation,profoundly reduced the glycosylation level in MIA PaCa-2 cells,suggesting that KRAS plays a role in the cellular glycosylation process,and thus glycosylation inhibition contributes to the anti-tumor effect of AMG-510.

关键词： Pancreatic cancer Glycosylation Biomarker Glycoproteomics Mass spectrometry

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A simple,rapid,and practical method for single-cell proteomics based on mass-adaptive coating of synthetic peptides

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Science Bulletin 2022年第6期67卷 581-584,M0003页

作者： Siqi Li Kaichan Su Zhenkun Zhuang Qing Qin Lei Gao Yamei Deng Xuyang Liu Guixue Hou Longtao Wang piliang hao Huanming Yang Siqi Liu Hongming Zhu Yan Ren BGI-Shenzhen Shenzhen 528083China Department of Biology University of CopenhagenCopenhagen 2100Denmark BGI-Wuhan Clinical Laboratories BGI-ShenzhenWuhan 430000China Department of Cardiology Zhongshan HospitalFudan UniversityShanghai Institute of Cardiovascular DiseaseShanghai 200032China Department of Cardiovascular Surgery The First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of China(USTC)Hefei 230001China School of Life Science and Technology Shanghai Tech UniversityShanghai 201210China Institute for Regenerative Medicine Shanghai East HospitalTongji University School of MedicineShanghai 200120China Experiment Center for Science and Technology Shanghai University of Traditional Chinese MedicineShanghai 201203China

Since a typical mammalian cell contains only 200–300 picograms of proteins and the lack of a proteome amplification strategy[1],a practical and high-throughput approach for single-cell proteomics has long been awaited.A series of methods have been explored to make single-cell proteomics *** design of nanoPOTS and OAD chip-based processes minimizes sample preparation steps and volumes to reduce sample loss[2,3].

关键词：乙醛脱氢酶2 单细胞蛋白质蛋白质组学潜在作用质谱数据数据采集模式 HeLa细胞快速检测方法

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甘肃省56万例新生儿苯丙酮尿症筛查结果分析

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国际检验医学杂志 2015年第24期36卷 3588-3590页

作者：王兴郝胜菊陈丕亮冯暄闫有圣甘肃省妇幼保健院医学遗传学中心甘肃兰州730050 甘肃省妇幼保健院新生儿疾病筛查中心甘肃兰州730050

目的回顾性分析甘肃省567 691例新生儿苯丙酮尿症筛查结果,了解甘肃省新生儿苯丙酮尿症（PKU）的发病情况,为甘肃省PKU的防治提供基础数据。方法标本为甘肃省新生儿筛查中心于2009~2014年收集的567 691例新生儿足跟血滤纸干血斑样品,用... 详细信息

目的回顾性分析甘肃省567 691例新生儿苯丙酮尿症筛查结果,了解甘肃省新生儿苯丙酮尿症（PKU）的发病情况,为甘肃省PKU的防治提供基础数据。方法标本为甘肃省新生儿筛查中心于2009~2014年收集的567 691例新生儿足跟血滤纸干血斑样品,用荧光定量法检测苯丙氨酸水平,并采用尿碟呤谱分析及苯丙氨酸羟化酶（PAH）基因突变检测进行鉴别诊断。结果在567 691例新生儿中,确诊PKU患儿166例;总体检出率为1/3 420,其中经典PKU 119例（71.7%）,中等型PKU33例（19.9%）,轻型PKU 14例（8.4%）。结论甘肃省PKU发病率远高于全国平均发病水平,以经典型PKU发病为主,应成为PKU重点防治地区。

关键词：新生儿疾病筛查苯丙酮尿症荧光定量法甘肃

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