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Mixing Efficiency across Rayleigh-Taylor and Richtmeyer-Meshkov Fronts

Open Journal of Fluid Dynamics 2015年第2期5卷 145-150页

作者： Jose Manuel Redondo Pilar lopez Gonzalez-Nieto Jose Leandro Cano German Andres Garzon Applied Physics Department Politecnica University of Catalu&#241 a (UPC) Barcelona Spain Applied Mathematics Department Complutense University of Madrid (UCM) Madrid Spain Earth Physics Astronomy and Astrophsics II Department Complutense University of Madrid (UCM) Madrid Spain

Mixing generated by gravitational acceleration and the role of local turbulence measured through multifractal methods is examined in numerical experiments of Rayleigh-Taylor and Richtmyer-Meshkov driven front occurring at density interfaces. The global advance of the fronts is compared with laboratory experiments and Nusselt and Sherwood numbers are calculated in both large eddy simulation (LES) and kinematic simulation KS models. In this experimental method, the mixing processes are generated by the evolution of a discrete set of forced turbulent plumes. We describe the corresponding qualitative results and the quantitative conclusions based on measures of the density field and of the height of the fluid layers. We present an experimental analysis to characterize the partial mixing process. The conclusions of this analysis are related to the mixing efficiency and the height of the final mixed layer as functions of the Atwood number, which ranges from 9.8 × 10−3 to 1.34 × 10−1.

关键词： Rayleigh-Taylor Instability Richtmyer-Meshkov Instability Mixing Efficiency Front Evolution Fractal Dimension

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Evaluating the potential of(epi)genotype‑by‑low pass nanopore sequencing in dairy cattle:a study on direct genomic value and methylation analysis

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Journal of Animal Science and Biotechnology 2023年第6期14卷 2276-2289页

作者： Oscar gonzalez‑Recio Adrian lopez‑Catalina Ramon Peiro‑Pastor Alicia nieto‑Valle Monica Castro Almudena Fernandez Dpt.Mejora Genetica Animal INIA-CSICCtra La Coruna Km 7.528040 MadridSpain. ETSIAAB Universidad Politecnica de Madrid.Ciudad Universitaria S/N28040 MadridSpain.

Background Genotype-by-sequencing has been proposed as an alternative to SNP genotyping arrays in genomic selection to obtain a high density of markers along the genome.It requires a low sequencing depth to be cost effective,which may increase the error at the genotype assigment.Third generation nanopore sequencing technology offers low cost sequencing and the possibility to detect genome methylation,which provides added value to genotype-by-sequencing.The aim of this study was to evaluate the performance of genotype-by-low pass nanopore sequencing for estimating the direct genomic value in dairy cattle,and the possibility to obtain methylation marks simultaneously.Results Latest nanopore chemistry(LSK14 and Q20)achieved a modal base calling accuracy of 99.55%,whereas previous kit(LSK109)achieved slightly lower accuracy(99.1%).The direct genomic value accuracy from genotype-by-low pass sequencing ranged between 0.79 and 0.99,depending on the trait(milk,fat or protein yield),with a sequencing depth as low as 2×and using the latest chemistry(LSK114).Lower sequencing depth led to biased estimates,yet with high rank correlations.The LSK109 and Q20 achieved lower accuracies(0.57-0.93).More than one million high reliable methylated sites were obtained,even at low sequencing depth,located mainly in distal intergenic(87%)and promoter(5%)regions.Conclusions This study showed that the latest nanopore technology in useful in a LowPass sequencing framework to estimate direct genomic values with high reliability.It may provide advantages in populations with no available SNP chip,or when a large density of markers with a wide range of allele frequencies is needed.In addition,low pass sequencing provided nucleotide methylation status of>1 million nucleotides at≥10×,which is an added value for epigenetic studies.

关键词： Genomic selection Genomic values Low pass sequencing Low sequencing imputation Polygenic risk score

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Selective sphincteroplasty of the papilla in cases at risk due to atypical anatomy

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World Journal of Gastroenterology 2007年第22期13卷 3106-3111页

作者： F Mugica G Urdapilleta A Castiella A Berbiela F Alzate E Zapata L Zubiaurre P lopez JI Arenas Gastroenterology Department Donostia Hospital San Sebastian Spain Department Donostia Hospital San Sebastian Spain Gastroenterology Department Bajo Deva Hospital Spain Anaesthesia Department Donostia Hospital San Sebastian Spain

AIM: To analyze the indications, effi cacy and safety of sphincteroplasty in our centre. METHODS: A retrospective study of sphincteroplasty in 53 cases of papilla at high risk was performed in 2004-2006. The procedure consisted of duodenoscopy with Olympus TJF 145 Videoduodenoscope, approach to the biliary tract using a catheter with a guidewire, and dilatation of the papilla with a dilatation balloon catheter using a syringe with a manometer for control of the fi lling pressure. RESULTS: The indications included intradiverticular papilla in 26 patients (49/), stenosis of a previous sphincterotomy in 19 patients (35.8/), small size of the papilla in 4 patients (7.5/), Billroth Ⅱ gastrectomy in 3 patients (5.6/), and coagulopathy in one patient (1.9/). The efficacy was 97.8/, with all the calculi extracted from the common bile duct in 84.4/ of the patients, even though 21 of the patients (39.6/) had calculi with a diameter equal to or greater than 10 mm. Seven patients (13.2/) presented complications: haemorrhage in 1 patient (1.9/) and mild pancreatitis in 6 patients (11.3/). The mean hospital stay in case of complications was of 3 ± 0.63 d. CONCLUSION: Sphincteroplasty is highly effective, with a complication rate similar to that of sphincterotomy, furthermore, the complications are of low clinical importance. The use of the 10 mm balloon makes it possible to extract calculi with a diameter of over 15 mm and to extract more than 3 calculi without increasing the rate of complications and reduces the need to resort tolithotripsy or rescue sphincterotomy.

关键词：括约肌切开术解剖学胆总管结石病急性胰腺炎乳头

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