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Evaluation of the Safety of Three Phenolic Compounds from <i>Dipteryx alata</i>Vogel with Antiophidian Potential

Chinese Medicine 2015年第1期6卷 1-12页

作者： Edson Hideaki Yoshida Miriéle Cristina Ferraz Natália Tribuiani Renata Vasques da Silva Tavares José Carlos Cogo Márcio Galdino dos Santos Luiz Madaleno Franco Cháriston André Dal-Belo Rone A. De Grandis Flávia Aparecida Resende Eliana Aparecida Varanda pilar puebla Arturo San-Feliciano Francisco Carlos Groppo Yoko Oshima-Franco Post-Graduate Program in Pharmaceutical Sciences University of Sorocaba Sorocaba Brazil Serpentarium of the University of Vale do Paraíba S&atildeo José dos Campos Brazil Post-Graduate Program in Environmental Sciences Tocantins Federal University Palmas Brazil Methodist University of Piracicaba Piracicaba Brazil Laboratory of Neurobiology and Toxinology Federal University of Pampa S&atildeo Gabriel Brazil Department of Biological Sciences S&atildeo Paulo State University Araraquara Brazil Department of Pharmaceutical Chemistry Salamanca University Salamanca Spain Department of Physiological Sciences University of Campinas Piracicaba Brazil

Phenolic compounds from Dipteryx alata Vogel were assayed against the in vitro neurotoxic effect induced by Bothrops jararacussu (Bjssu) venom. Mutagenicity was assessed by the Ames test using Salmonella typhimurium strains TA98, TA97a, TA100, and TA102, in experiments with and without metabolic activation. Anti-bothropic activity was obtained by using mouse phrenic nerve-diaphragm (PND) preparation and myographic technique. Control experiments with physiological Tyrode solution were used for keeping the PND preparations alive (n = 4). Concentrations of phe-nolic compounds were as follow: protocatechuic and vanillic acids (200 μg/mL, n = 4), vanillin (50 μg/mL, n = 4). These compounds were used alone or pre-incubated with the venom (40 μg/mL), 30 min prior the addition to the organ bath (n = 4). Phenolic compounds significantly inhibited the neuromuscular blockade of Bjssu in the following order of potency: vanillic acid > protocatechuic = vanillin. Vanillic acid added 10 min after the Bjssu venom was also able to avoid the venomblockade evolution. The mutagenicity assay indicated that all phytochemicals were unable to in-crease the number of revertants, demonstrating the absence of mutagenic activity. This study demonstrated both the safety and therapeutical potential of the three phenolic compounds as novel complementary anti-bothropic agents.

关键词： Ames Test Baru Bothrops jararacussu Venom Vanillic Acid Vanillin

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