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Gene expression changes in spleens of the wildlife reservoir species,Eurasian wild boar(Sus scrofa),naturally infected with Brucella suis biovar 2

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Journal of Genetics and Genomics 2010年第11期37卷 725-736页

作者： Ruth C.Galindo pilar m.muoz maría J.de miguel Clara m.marin Javier Labairu miguel Revilla José m.Blasco Christian Gortazar José de la Fuente Instituto de Investigación en Recursos Cinegéticos IREC(CSIC-UCLm-JCCm) Unidad de Sanidad Animal Centro de Investigación y Tecnología Agroalimentaria(CITA) ITG Ganadero Avda. Serapio Huici 2231610 VillabaNavarraSpain Departamento de Patología Animal Facultad de VeterinariaUniversity of Zaragoza Department of Veterinary Pathobiology Center for Veterinary Health SciencesOklahoma State University

Brucella suis is responsible for swine brucellosis worldwide. Of the five different B. suis biovars （by.）, bv. 2 appears restricted to Europe where it is frequently isolated from wild boar and hares, can infect pigs and can cause human brucellosis. In this study, the differ- ential gene expression profile was characterized in spleens of Eurasian wild boar naturally infected with B. suis bv. 2. Of the 20,201 genes analyzed in the microarray, 633 and 1,373 were significantly （fold change 〉 1.8; P 〈 0.01） upregulated and downregulated, respectively, in infected wild boar. The analysis was focused on genes that were over represented after conditional test for biological process gene ontology. Upregulated genes suggested that B. suis bv. 2 infection induced cell maturation, migration and/or proliferation in infected animals. The genes downregulated in infected wild boar impaired the activity of several important cellular metabolic pathways such as metabolism, cytoskeleton organization and biogenesis, immune response and lysosomal function and vesicle-mediated transport. In addition, the response to stress, sperm fertility, muscle development and apoptosis seemed to be also impaired in infected animals. These results suggested that B. suis bv. 2 may use strategies similar to other smooth brucellae to facilitate intracellular multiplication and the development of chronic infections. To our knowledge, this is the first report of the analysis of gene expression profile in hosts infected with B. suis bv. 2, which is important to understand the molecular mechanisms at the host-pathogen interface in the main reservoir species with possible implications in the zoonotic cycle of the pathogen.

关键词： Brucella suis boar genomics microarray systems biology

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Remediation of amide pesticide-polluted soils by combined solarization and ozonation treatment

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Pedosphere 2024年第3期34卷 641-651页

作者： Isabel GARRIDO Carmen mmARTINEZ pilar FLORES pilar HELLIN Fulgencio CONTRERAS Jose FENOLL Department of Sustainability and Quality Group of Fruit and Vegetable Products Instituto Murciano de Investigacion y Desarrollo Agrario y MedioambientalLa Alberca 30150(Spain)

Agriculture has a close relationship with nature,but it can also be the source of negative and permanent environmental *** use of pesticides in modern agriculture is a common practice,but their side effects on the environment cannot be *** this study,we evaluated a combination of solarization and ozonation techniques for the elimination of six amide pesticides(boscalid,chlorantraniliprole,cyflufenamid,fluopyram,napropamide,and propyzamide)in *** experiments were performed with four different soils to assess the efficiency of this methodology at different soil temperatures and ozone dosages under laboratory conditions,and then a greenhouse pot experiment was conducted under controlled conditions during *** days after the onset of the experiments,higher degradation percentages of amide pesticides were observed in ozonized soils than in other treated soils,particularly when ozone was applied at 10 cm soil *** results show that the utilization of ozonation,along with solarization,represents a valid method for degrading residues of the studied pesticides and suggest that this combined technology may be a promising tool for remediating pesticide-polluted soils.

关键词： intermediate ozone pesticide degradation pesticide residue polyethylene film remediation technique

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morphometric and kinematic sperm subpopulations in split ejaculates of normozoospermic men

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Asian Journal of Andrology 2016年第6期18卷 831-834页

作者： pilar Santolaria Caries Soler pilar Recreo Teresa Carretero Araceli Bono Jose m Berne Jesus L Yaniz TECNOGAm research group Environmental Sciences Institute (IUCA) Department of Animal Production and Food Sciences University of Zaragoza Huesca Spain Department of Functionat 8iotogy and Physical Anthropology University of Valencia 46100 Burjassot Valencia Spain R+D Department Proiser R+D Scientific Park University of Valencia 46980 Paterna Valencia Spain TECNOGAm research group Environmental Sciences Institute (IUCA) Departament of Human Anatomy and Histolosy Faculty of Health and Sport Sciences of Huesca PLaza Universidad 3 22002 Huesca Spain TECNOGAm research group Environmental Sciences Institute (IUCA) Hospital General San Jorge de Huesca Area de Urologia Av. Martinez de Velasco 36 22004 Huesca Spain

This study was designed to analyze the sperm kinematic and morphometric subpopulations in the different fractions of the ejaculate in normozoospermic men. Ejaculates from eight normozoospermic men were collected by masturbation in three fractions after 3-5 days of sexual abstinence. Analyses of sperm motility by computer-assisted sperm analysis （CASA-mot）, and of sperm morphometry by computer-assisted sperm morphometry analysis （CASA-morph） using fluorescence were performed. Clustering and discriminant procedures were performed to identify sperm subpopulations in the kinematic and morphometric data obtained. Clustering procedures resulted in the classification of spermatozoa into three kinematic subpopulations （slow with low ALH [35.6% of all motile spermatozoa], with circular trajectories [32.0%], and rapid with high ALH [32.4%]）, and three morphometric subpopulations （large-round [33.9% of all spermatozoa], elongated [32.0%], and small [34.10%]）. The distribution of kinematic sperm subpopulations was different among ejaculate fractions （P 〈 0.001）, with higher percentages of spermatozoa exhibiting slow movements with low ALH in the second and third portions, and with a more homogeneous distribution of kinematic sperm subpopulations in the first portion. The distribution of morphometric sperm subpopulations was also different among ejaculate fractions （P〈 0.001）, with more elongated spermatozoa in the first, and of small spermatozoa in the third, portion. It is concluded that important variations in the distribution of kinematic and morphometric sperm subpopulations exist between ejaculate fractions, with possible functional implications.

关键词： man sperm morphometry by computer-assisted sperm morphometry analysis sperm motility by computer-assisted spermanalysis sperm subpopulations split ejaculate

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New genes emerging for colorectal cancer predisposition

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World Journal of Gastroenterology 2014年第8期20卷 1961-1971页

作者： Clara Esteban-Jurado pilar Garre maria Vila Juan José Lozano Anna Pristoupilova Sergi Beltrán Anna Abulí Jenifer muoz Francesc Balaguer Teresa Ocaa Antoni Castells Josep m Piqué Angel Carracedo Clara Ruiz-Ponte Xavier Bessa montserrat Andreu Luis Bujanda Trinidad Caldés Sergi Castellví-Bel Department of Gastroenterology Hospital ClínicCentro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas(CIBEREHD)Institut d’Investigacions Biomèdiques August Pi i Sunyer(IDIBAPS)University of Barcelona08036 BarcelonaCataloniaSpain molecular Oncology Laboratory Hospital Clínico San CarlosInstituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC)28040 MadridSpain Bioinformatics platform Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD)08036 BarcelonaSpain Centre Nacional d’Anàlisi Genòmica (CNAG) Parc Científic de Barcelona08028 BarcelonaSpain Department of Gastroenterology Hospital del Mar-IMIM (Hospital del Mar Medical Research Centre)Pompeu Fabra University08003 BarcelonaSpain Galician Public Foundation of Genomic medicine (FPGmX) Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER)Genomics Medicine GroupHospital ClínicoSantiago de CompostelaUniversity of Santiago de Compostela15706 GaliciaSpain Center of Excellence in Genomic medicine Research King Abdulaziz University21589 JeddahKingdom of Saudi Arabia Gastroenterology Department Hospital DonostiaNetworked Biomedical Research Centre for Hepatic and Digestive Diseases (CIBEREHD)Basque Country University20080 San SebastiánSpain

Colorectal cancer(CRC)is one of the most frequent neoplasms and an important cause of mortality in the developed *** cancer is caused by both genetic and environmental factors although 35%of the variation in CRC susceptibility involves inherited genetic *** syndromes account for about5%of the total burden of CRC,with Lynch syndrome and familial adenomatous polyposis the most common *** hereditary forms,there is an important fraction of CRC cases that present familial aggregation for the disease with an unknown germline genetic *** can be also considered as a complex disease taking into account the common diseasecommom variant hypothesis with a polygenic model of inheritance where the genetic components of common complex diseases correspond mostly to variants of low/moderate *** far,30 common,low-penetrance susceptibility variants have been identified for ***,new sequencing technologies including exomeand whole-genome sequencing have permitted to add a new approach to facilitate the identification of new genes responsible for human disease *** using whole-genome sequencing,germline mutations in the POLE and POLD1 genes have been found to be responsible for a new form of CRC genetic predisposition called polymerase proofreading-associated polyposis.

关键词： Colorectal neoplasm, genetic predisposition to disease Next generation sequencing Genotype-phenotype correlation Genetic variant Single nucleotide polymorphism

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Type XIX collagen: a promising biomarker from the basement membranes

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Neural Regeneration Research 2020年第6期15卷 988-995页

作者： Ana C.Calvo Laura moreno Leticia moreno Janne m.Toivonen Raquel manzano Nora molina miriam de la Torre Tresa López Francisco J.miana-mena maría J.munoz pilar Zaragoza pilar Larrodé Alberto García-Redondo Rosario Osta Laboratory of Genetics and Biochemistry(LAGENBIO) University of ZaragozaFaculty of Veterinary SciencesInstituto de Investigación Sanitaria de Aragón(IIS)IA2CIBERNEDZaragozaSpain Hospital Clínico Universitario Lozano Blesa ZaragozaSpain Neurology Department ALS UnitCIBERER U-723Health Research InstituteMadridSpain

Among collagen members in the collagen superfamily,type XIX collagen has raised increasing interest in relation to its structural and biological *** XIX collagen is a Fibril-Associated Collagen with Interrupted Triple helices member,one main subclass of collagens in this *** collagen contains a triple helix composed of three polypeptide segments aligned in parallel and it is associated with the basement membrane zone in different *** molecular structure of type XIX collagen consists of five collagenous domains,COL1 to COL5,interrupted by six non-collagenous domains,NCI to *** most relevant domain by which this collagen exerts its biological roles is NCI domain that can be cleavage enzymatically to release matricryptins,exerting anti-tumor and anti-angiogenic effect in murine and human models of *** physiological conditions,type XIX collagen expression decreases after birth in different tissues although it is necessary to keep its basal levels,mainly in skeletal muscle and hippocampal and telencephalic interneurons in ***,in amyotrophic lateral sclerosis,altered transcript expression levels show a novel biological effect of this collagen beyond its structural role in basement membranes and its anti-tumor and anti-angiogenic *** XIX collagen can exert a compensatory effect to ameliorate the disease progression under neurodegenerative conditions specific to amyotrophic lateral sclerosis in transgenic SOD1 G93 A mice and amyotrophic lateral sclerosis *** novel biological role highlights its nature as prognostic biomarker of disease progression in and as promising therapeutic target,paving the way to a more precise prognosis of amyotrophic lateral sclerosis.

关键词： anti-tumor and anti-angiogenic properties C1 domain COL19A1 gene and protein levels compensatory effect FACIT collagens hippocampal interneurons matricryptins multiplexins NC1 domain regenerative response skeletal muscle type XIX collagen

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Comparison of different statistical approaches to evaluate morphometric sperm subpopulations in men

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Asian Journal of Andrology 2016年第6期18卷 819-823页

作者： Jesus L Yaniz Sandra Vicente-Fiel Caries Soler pilar Recreo Teresa Carretero Araceli Bono Jose m Berne pilar Santolaria TECNOGAm research group Environmental Sciences Institute (IUCA) Department of Animal Production and Food Sciences Higher Polytechnic School University of Zaragoza Ctra. Cuarte S/N 22071 Huesca Spain Department of Functional Biology and Physical Anthropology University of Valencia 46100 Burjassot Valencia Spain R+D Department Proiser R+D Scientific Park University of Valencia 46980 Paterna Valencia Spain TECNOGAm research group Environmental Sciences Institute (IUCA) Departament of Human Anatomy and Histology Faculty of Health and Sport Sciences of Huesca Plaza Universidad 3 22002 Huesca Spain TECNOGAm research group Environmental Sciences Institute (IUCA) Hospital General San Jorge de Huesca Area de Urologla Av. Martfnez de Velasco 36 22004 Huesca Spain

This study was designed to characterize morphometric sperm subpopulations in normozoospermic men by using different statistical methods and examining their suitability to classify correctly different sperm nuclear morphologies present in human ejaculates. Ejaculates from 21 normozoospermic men were collected for the study. After semen collection and analysis, samples were prepared for morphometric determination. At least 200 spermatozoa per sample were assessed for sperm morphometry by computer-assisted sperm morphometry analysis （CASA-morph） using fluorescence. Clustering and discriminant procedures were performed to identify sperm subpopulations from the morphometric data obtained. Clustering procedures resulted in the classification of spermatozoa into three morphometric subpopulations （large-round 30.4%, small-round 46.6%, and large-elongated 22.9%）. In the second analysis, using discriminant methods, the classification was made independently of size and shape. Three morphological categories according to nuclear size （small 〈10.90 μm^2, intermediate 10.91-13.07 μm^2, and large 〉13.07 μm^2） and four categories were defined on 400 canonical cells （100 × 4） from 10 men according to sperm nuclear shape （oval, pyriform, round, and elongated）. Thereafter, the resulting classification functions were used to categorize 4200 spermatozoa from 21 men. Differences in the class distribution were observed among men from both clustering and discriminant procedures. It was concluded that the combination of CASA-morph fluorescence-based technology with multivariate cluster or discriminant analyses provides new information on the description of different morphometric sperm subpopulations in normal individuals, and that important variations in the distribution of morphometric sperm subpopulations may exist between men, with possible functional implications.

关键词： computer-assisted sperm morphometry analysis man sperm subpopulations

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Heritage Conservation in Postcolonial India

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Built Heritage 2022年第1期6卷 110-112页

作者： pilar m.Guerrieri Department DASTU Politecnico Di MilanoEdoardo Bonardi32013320133 MilanoMIltaly

Heritage Conservation in Postcolonial *** and Challenges,edited by manish Chalana and Ashima Krishna,is very rich,and counts thirteenth different con-tributors among urban and architectural conservationists,urban and historic preservation plan-ners,landscape designers and architects,social entrepre-neurs,experts in cultural heritage from universities,social and research institutions.

关键词： architectural landscape coloni

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Efficiency of white lupin in the removal of mercury from contaminated soils: Soil and hydroponic experiments

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Journal of Environmental Sciences 2010年第3期22卷 421-427页

作者： pilar Zornoza Rocío milln m. Jos Sierra Almudena Seco Elvira Esteban Department of Agricultural Science Autonomous University of Madrid Francisco Tomsy Valiente 7. E-28049 Madrid Spain Research Center for Energy Environment and Technology Environmental Department. Soil Degradation Research Unit. Av. Complutense 22. E-28040 Madrid Spain

This study examined the ability of the white lupin to remove mercury （Hg） from a hydroponic system （Hg concentrations 0, 1.25, 2.5, 5 and 10 μmol/L） and from soil in pots and lysimeters （total Hg concentration （19.2 ± 1.9） mg/kg availability 0.07%, and （28.9 ± 0.4） mg/kg availability 0.09%, respectively）, and investigated the accumulation and distribution of Hg in different parts of the plant. White lupin roots efficiently took up Hg, but its translocation to the harvestable parts of the plant was low. The Hg concentration in the seeds posed no risk to human health according to the recommendations of the World Health Organization, but the shoots should not be used as fodder for livestock, at least when unmixed with other fodder crops. The accumulation of Hg in the hydroponically-grown plants was linear over the concentration range tested. The amount of Hg retained in the roots, relative to the shoots, was almost constant irrespective of Hg dose （90%）. In the soil experiments, Hg accumulation increased with exposure time and was the greater in the lysimeter than in the pot experiments. Although Hg removal was the greater in the hydroponic system, revealing the potential of the white lupin to extract Hg, bioaccumulation was the greatest in the lysimeter-grown plants; the latter system more likely reflects the true behaviour of white lupin in the field when Hg availability is a factor that limits Hg removal. The present results suggest that the white lupin could be used in long-term soil reclamation strategies that include the goal of profitable land use in Hg-polluted areas.

关键词： edible plant parts hydroponic-soil-lysimeter experiments Lupinus albus L. mercury

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Female mate choice based on pheromone content may inhibit reproductive isolation between distinct populations of Iberian wall lizards

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Current Zoology 2013年第2期59卷 210-220页

作者： marianne GABIROT pilar LOPEZ Jose mARTIN Departement Ecologie Comportementale (U.M.R. 5175) CEFE-CNRS 1919 Route de Mende F34293 Montpellier Cedex 5 France Departamento de Ecologia Evolutiva Museo Naeional de Ciencias Naturales C.S.I.C. Jos6 Guti6rrez Abascal 2 28006 Madrid Spain

The Iberian wall lizard Podarcis hispanica forms part of a species complex with several morphologically and geneti- cally distinct types and populations, which may or may not be reproductively isolated. We analyzed whether female mate choice based on males＇ chemical signals may contribute to a current pre-mating reproductive isolation between two distinct populations of P. hispanica from central Spain. We experimentally examined whether females choose to establish territories on areas scent-marked by males of their own population, versus areas marked by males of the other population. Results showed that fe- males did not prefer scent-marks of males from their own population. In contrast, females seemed to attend mostly to among-individual variation in males＇ pheromones that did not differ between populations. Finally, to test for strong premating re- productive isolation, we staged intersexual encounters between males and females. The population of origin of males and females did not affect the probability nor the duration of copulations. We suggest that the different environmental conditions in each population might be selecting for different morphologies and different chemical signals of males that maximize efficiency of communication in each environment. However, females in both populations based mate choice on a similar condition-dependent signal of males. Thus, male signals and female mate choice criteria could be precluding premating reproductive isolation between these phenotypically ＂distinct＂ populations [Current Zoology 59 （2）： 210-220, 2013].

关键词： Podarcis hispanica Chemical communication mate preferences mate recognition Species recognition Conflict

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microstructural Feature of As-cast A-356 Alloy modifiers with the Addition of SrO

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Journal of Wuhan University of Technology(materials Science) 2014年第3期29卷 617-619页

作者： mu?IZ Rodrigo FLORES Alfredo ORTIZ Juan RODRíGUEZ Nelly mARTíNEZ Fernando ORozCO pilar LUNA Jesús Equihua Fabián García Adrián Faculty of Engineering Autonomous University of Coahuila Camporedondo UnitSaltillo Coahuila México CINVESTAV Saltillo Unit Department of metal mechanics Technological Institute of Saltillo Faculty of Engineering Autonomous University of Zacatecas FImE Autonomous University of Coahuila Unit

To explore the effect of strontium on the structure of as-cast A356 alloy, the strontium was incorporated to the alloy by metallothermic reduction of SrO where the mineral was added to the melt through the submerged powders injection technique. The evaluation of the modification of the eutectie silicon and the chemical analysis of samples were done using optical microscopy （OP） and inductively coupled plasma （ICP）, respectively, while microstructural analyses by scanning electron microscopy （SEm）, and the injection time was variable. magnesium was added to the melt to increase the reactivity and reduce the surface tension of the molten aluminum. It was possible to increase the strontium content from 0 to 0.027% after 20 minutes treatment. This concentration was sufficient to bring about full modification structure of eutectic silicon of as cast alloy A 356 and the acceptable quality metallurgical of alloy.

关键词： modification microstmcture strontium eutectic silicon submerged powder injection

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