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Bioelectrochemically-assisted degradation of chloroform by a coculture of Dehalobacter and Dehalobacterium

Environmental science and Ecotechnology 2022年第4期12卷 81-86页

作者： David Fernandez-Verdejo pilar cortés.albert guis.s.la Paqui Blánquez Ernest Marco-Urrea Biorem UAB Department of ChemicalBiological and Environmental EngineeringSchool of EngineeringUniversitat Autonoma de Barcelona08193BellaterraBarcelonaSpain Departament de Genetica i de Microbiologia Facultat de BioCienciesUniversitat Autonoma de Barcelona08193BellaterraBarcelonaSpain GENOCOV Department of ChemicalBiological and Environmental EngineeringSchool of EngineeringUniversitat Autonoma de Barcelona08193BellaterraBarcelonaSpain

Using bioelectrochemical systems(BEss)to provide electrochemically generated hydrogen is a promising technology to provide electron donors for reductive dechlorination by organohalide-respiring *** this study,we inoculated two syntrophic dechlorinating cultures containing Dehalobacter and Dehalobacterium to sequentially transform chloroform(CF)to acetate in a BEs using a graphite fiber brush as the *** this co-culture,Dehalobacter transformed CF to stoichiometric amounts of dichloromethane(DCM)via organohalide respiration,whereas the Dehalobacterium-containing culture converted DCM to acetate via *** were initially inoculated with Dehalobacter,and sequential cathodic potentials of-0.6,-0.7,and -0.8 V were poised after consuming three CF doses(500 μM)per each potential during a time-span of 83 *** the end of this period,the accumulated DCM was degraded in the following seven days after the inoculation of *** this point,four consecutive amendments of CF at increasing concentrations of 200,400,600,and 800 μM were sequentially transformed by the combined degradation activity of Dehalobacter and *** Dehalobacter 16s rRNA gene copies increased four orders of magnitude during the whole *** coulombic efficiencies associated with the degradation of CF reached values>60% at a cathodic potential of -0.8 V when the degradation rate of CF achieved the highest *** study shows the advantages of combining syntrophic bacteria to fully detoxify chlorinated compounds in BEss and further expands the use of this technology for treating water bodies impacted with pollutants.

关键词： Dehalobacter Dehalobacterium Chloroform Dichloromethane Bioelectrochemistry Bioremediation

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Diagnosis of Liver Involvement in Primary sj(o)gren syndrome

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Journal of Clinical and Translational Hepatology 2013年第2期1卷 94-102页

作者： pilar Brito Zeron soledad Retamozo albert Bové Belchin Adriyanov Kostov Antoni sisó Manuel Ramos-Casals sj(o)gren syndrome Research Group(AGAUR) Laboratory of Autoimmune Diseases Josep Font Institut d'Investigacions Biomèdiques August Pi i Sunyer(IDIBAPS) Department of Autoimmune Diseases ICMiD Hospital Clinic Barcelona Spain Primary Care Research Group IDIBAPS Centre d'Assistència_Primària ABS Les Corts GESCLINIC Barcelona Spain

Liver involvement was one of the first extraglandular manifestations to be reported in patients with primary sj(o)gren syndrome (ss).In the 1990s,a study of liver involvement in patients with primary ss integrated the evaluation of clinical signs of liver disease,liver function and a complete panel of *** developments in the field of hepatic and viral diseases have significantly changed the diagnostic approach to liver involvement in *** most recent studies have shown that,after eliminating hepatotoxic drugs and fatty liver disease,the two main causes of liver disease in primary ss are chronic viral infections and autoimmune liver *** differential diagnosis of liver disease in primary ss (viral vs autoimmune) is clinically important,since the two processes require different therapeutic approaches and have different *** respect to viral infections,chronic HCV infection is the main cause of liver involvement in ss patients from the Mediterranean area,while chronic HBV infection may be the main cause of liver involvement in ss patients from Asian *** eliminating viral hepatitis,primary biliary cirrhosis (PBC) should be considered the main cause of liver disease in primary ***-related ss patients may have a broad spectrum of abnormalities of the liver,including having no clinical or analytical data suggestive of liver *** hepatitis (AIH) is the second most frequently found autoimmune liver disease to be associated with ss (all reported cases are type I),and nearly 10% of these patients have an AIH-PBC ***,IgG4-related disease must be investigated in patients with ss presenting with sclerosing cholangitis,especially when autoimmune pancreatitis or retroperitoneal fibrosis are also present.

关键词： sj(o)gren syndrome Liver disease Hepatitis B virus Hepatitis C virus Primary biliary cirrhosis Autoimmune hepatitis sclerosing cholangitis

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Acute Respiratory Distress syndrome (ARDs) Case Report after a Caesarean section Related to the Immune Reconstitution Inflammatory syndrome

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Open Journal of Anesthesiology 2014年第8期4卷 197-201页

作者： álvaro Ramiro Ruiz pilar Reboto cortés Ignacio García sánchez Juan Emilio Losa Miguel Miró Murillo santiago García del Valle y Manzano Hospital Universitario Fundación Alcorcón Madrid Spain

During pregnancy, a cellular immunosuppressant status is produced. It is characterized by anti-inflammatory cellular responses that allow embryonic implantation. The reversal of these changes during the postpartum period may result in overt clinical manifestations of otherwise quiescent or latent infections. We present an Acute Respiratory Distress syndrome (ARDs) case after a caesarean section related to the Immune Reconstitution Inflammatory syndrome (IRIs) in a Guinean black woman. The patient was treated with large doses of corticosteroids showing a great clinical and radiological improvement.

关键词： Immune Reconstitution Inflammatory syndrome Mycobacterium Tuberculosis Pregnancy

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Bioelectrochemically-assisted degradation of chloroform by a co-culture of Dehalobacter and Dehalobacterium

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"Environmental science and Ecotechnology" 2022年第4期 41-46页

作者： David Fernández-Verdejo pilar cortés.albert guis.s.la Paqui Blánquez Ernest Marco-Urrea Biorem UAB Department of Chemical Biological and Environmental Engineering School of Engineering Universitat Autònoma de Barcelona Departament de Genètica i de Microbiologia Facultat de BioCiències Universitat Autònoma de Barcelona GENOCOV Department of Chemical Biological and Environmental Engineering School of Engineering Universitat Autònoma de Barcelona

Using bioelectrochemical systems(BEss) to provide electrochemically generated hydrogen is a promising technology to provide electron donors for reductive dechlorination by organohalide-respiring bacteria. In this study, we inoculated two syntrophic dechlorinating cultures containing Dehalobacter and Dehalobacterium to sequentially transform chloroform(CF) to acetate in a BEs using a graphite fiber brush as the electrode. In this co-culture, Dehalobacter transformed CF to stoichiometric amounts of dichloromethane(DCM) via organohalide respiration, whereas the Dehalobacterium-containing culture converted DCM to acetate via fermentation. BEs were initially inoculated with Dehalobacter, and sequential cathodic potentials of-0.6,-0.7, and-0.8 V were poised after consuming three CF doses(500 μM) per each potential during a time-span of 83 days. At the end of this period, the accumulated DCM was degraded in the following seven days after the inoculation of Dehalobacterium. At this point, four consecutive amendments of CF at increasing concentrations of 200, 400, 600, and 800 μM were sequentially transformed by the combined degradation activity of Dehalobacter and Dehalobacterium. The Dehalobacter 16s r RNA gene copies increased four orders of magnitude during the whole period. The coulombic efficiencies associated with the degradation of CF reached values > 60% at a cathodic potential of-0.8 V when the degradation rate of CF achieved the highest values. This study shows the advantages of combining syntrophic bacteria to fully detoxify chlorinated compounds in BEss and further expands the use of this technology for treating water bodies impacted with pollutants.

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