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The mRNA Binding Proteome of Proliferating and Differentiated Muscle Cells

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Genomics, Proteomics & Bioinformatics 2020年第4期18卷 384-396页

作者： Monika Hiller Miriam Geissler George Janssen Peter van Veelen Annemieke Aartsma-Rus pietro Spitali Department of Human Genetics Leiden University Medical CenterLeiden 2333 ZCthe Netherlands Center for Proteomics and Metabolomics Leiden University Medical CenterLeiden 2333 ZCthe Netherlands

Muscle formation is a coordinated process driven by extensive gene expression changes where single cells fuse together to form multinucleated muscle fibers. Newly synthesized m RNAs are then regulated by RNA binding proteins(RBPs), affecting post-transcriptional transcript metabolism. Here, we determined how large-scale gene expression changes affect the catalog of RBPs by studying proliferating and differentiated muscle cells in healthy and dystrophic conditions. Transcriptomic analysis showed that the expression of more than 7000 genes was affected during myogenesis. We identified 769 RBPs, of which 294 were muscle-specific and 49 were uniquely shared with cardiomyocytes. A subset of 32 RBPs(half of which were muscle-specific) was found to be preferentially associated with target mRNAs in either myoblasts(MBs) or myotubes(MTs). A large proportion of catalytic proteins were bound to mRNAs even though they lack classical RNA binding domains. Finally, we showed how the identification of cell-specific RBPs enabled the identification of biomarkers that can separate healthy individuals from dystrophic patients. Our data show how interactome data can shed light on new basic RNA biology as well as provide cell-specific data that can be used for diagnostic purposes.

关键词： RNA binding protein Interactome Duchenne muscular dystrophy Proteomics Skeletal muscle

Carotid endarterectomies for relieving severe or moderate carotid stenosis

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Chinese Medical Journal 2004年第6期117卷 956-958页

作者：吕志前萧明第 Montagna pietro Farhat Fadi Olivier Jegaden Department of Cardiovascular Surgery Shanghai First People's Hospital Department of Cardiovascular Surgery Lyon Cardiac Hospital

Carotid artery stenosis is one of the major causes of ischemic strokes.1 To prevent or reduce the occurrences of ischemic strokes, carotid endarterectomies (CEAs) were performed on 48 patients with carotid artery sten... 详细信息

关键词： carotid stenosis·endarterectomy carotid

Ablative therapies for intrahepatic cholangiocarcinoma

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Hepatobiliary Surgery and Nutrition 2018年第3期7卷 192-194页

作者： Antonio Giorgio pietro Gatti Paolo Matteucci Valentina Giorgio Interventional Ultrasound Unit Tortorella Clinical HospitalSalernoItaly Internal Medicine Division Ostuni HospitalOstuni BRItaly Radiation Oncology Institute Campus Biomedico UniversityRomeItaly Pediatric Gastroenterology Unit Sacred Heart UniversityPoliclinico GemelliRomeItaly

Among the 3 sub-types of cholangiocarcinoma (CH), [intrahepatic CH, hylar (CH) and extrahepatic (CH)], intrahepatic cholangiocarcinoma (ICC) accounts for up 8–10% of cholangiocarcinomas and 10–20% of all primary liv... 详细信息

关键词： carcinoma liver for

The gut-liver axis as a target of liver disease management

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Hepatobiliary Surgery and Nutrition 2021年第1期10卷 100-102页

作者： Claudia Mandato Anna Pia Delli Bovi pietro Vajro Department of Pediatrics AORN“Santobono-Pausilipon”NaplesItaly Pediatrics Department of Medicine and Surgery University of SalernoSalernoItaly

The interplay between the gut and liver,the so called“gut-liver axis”(GLA),plays a significant role in health and *** shown in Figure 1,the GLA is regulated by a number of equally relevant interacting factors including gut microbiota,integrity and function of the intestinal wall,antigen trafficking,mucosal innate immune response,and oxidative stress(1,2).In addition to undigested or unabsorbed food and microorganisms,the gut lumen contains also their fermentation/metabolic products,bile,mucus,and epithelial cells spontaneously exfoliated from the gastrointestinal *** latter is composed of various cell types counting immune cells,goblet cells,and enterocytes strongly connected by tight junctions and covered with a mucus layer that acts as a dynamic *** elucidation of a gut-vascular barrier(GVB)has revealed this structure to intervene to control gut bacteria and antigen translocation from the gut into the blood stream(3).Interestingly,diseases that are characterized or aggravated by impairment of the barrier function(so-called“leaky gut”)and/or particular patterns of the microbiome(e.g.,a lack of balance between Bacteroides and Firmicutes)are not restricted to the intestinal tract and *** relevant examples are represented by diabetes type 1,familial Mediterranean fever,obesity,cardiovascular disease,decreased bone-mass density,autism,dysregulation of immune response and systemic allergic diseases(1).

关键词： Figure gut latter

A LoRa-based protocol for connecting IoT edge computing nodes to provide small-data-based services

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Digital Communications and Networks 2022年第3期8卷 257-266页

作者： Kiyoshy Nakamura pietro Manzoni Alessandro Redondi Edoardo Longo Marco Zennaro Juan-Carlos Cano Carlos T.Calafate Universitat Politécnica de Valéncia ValenciaSpain Abdus Salam International Centre for Theoretical Physics(ICTP) TriesteItaly Politecnico di Milano MilanItaly

Data is becoming increasingly *** regularly interact with a variety of structured data,ranging from SQLite databases on the phone to personal sensors and open government ***“digital traces left by individuals through these interactions”are sometimes referred to as“small data”.Examples of“small data”include driving records,biometric measurements,search histories,weather forecasts and usage *** this paper,we present a flexible protocol called LoRaCTP,which is based on LoRa technology that allows data“chunks”to be transferred over large distances with very low energy *** provides all the mechanisms necessary to make LoRa transfer reliable by introducing a lightweight connection setup and allowing the ideal sending of an as-long-as necessary data *** designed this protocol as communication support for small-data edge-based IoT solutions,given its stability,low power usage,and the possibility to cover long *** evaluated our protocol using various data content sizes and communication distances to demonstrate its performance and reliability.

关键词： Small data Edge computing LoRa IoT

Assessing spatial variability and erosion susceptibility of soils in hilly agricultural areas in Southern Italy

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International Soil and Water Conservation Research 2020年第4期8卷 354-362页

作者： Carmen Maria Rosskopf Erika Di Iorio Luana Circelli Claudio Colombo pietro P.C.Aucelli University of Molise Department of Biosciences and Territoryc.da Fonte Lappone86090PescheISItaly University of Molise Department of AgriculturalEnvironmental and Food SciencesVia F.De Sanctis186100CampobassoCBItaly University of Napoli"Parthenope" Department of Environmental SciencesCentro Direzionale Isola C/480143NapoliItaly

Soil erosion is one of the main environmental problems in the Mediterranean *** problem is becoming even more important especially in Italy,in the Apennines,where severe erosive processes occur due to the action of concentrated running *** erodibility(K-Factor)of a soil,estimated using the Revised Universal Soil Loss Equation(RUSLE),is a measure of its susceptibility to erosion and depends on several soil properties such as organic matter,texture and permeability and *** assess the spatial variability of soil properties and soil erodibility in hilly agricultural areas and to investigate the relationships between soil features and landscape morphodynamics,a detailed study in Molise region(southern Italy),in a small drainange basin located along its hilly Adriatic flank,was carried *** this catchment,63 topsoil samples(A horizons)were collected and 10 soil profiles,forming a catena crossing 3 land units,were *** calculated K-Factors ranges between 0.012 and 0.048 t ha h ha-1 MJ-1 mm-1 indicating a complex spatial distribution,due to the several local pedological and geomorphological factors affecting soil *** results give clear evidence about the relationships among soil characteristics,soil erodibility and landscape morpho-dynamics(land units).Comparing the soil loss rates estimated for the study area with those reported in literature,a good correspondence can be observed only for the more stable land unit,not characterized by intense erosive *** proposed methodology is suitable to highlight areas characterized by similar morphodynamics features,and comparable soil erodibility,for a more effective spatialization of K factor.

关键词： Hillslope morphodynamics RUSLE equation K factor Mediterranean region Soil catena Land units

Evaluation of dietary addition of 2 essential oils from Achillea moschata,or their components (bornyl acetate,camphor,and eucalyptol) on in vitro ruminal fermentation and microbial community composition

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Animal Nutrition 2021年第1期7卷 224-231页

作者： Stefania Colombini Andrea Rota Graziosi pietro Parma Marcello Iriti Sara Vitalini Chiara Sarnataro Mauro Spanghero Dipartimento di Scienze Agrarie e Ambientali—Produzione TerritorioAgroenergiaUniversitàDegli Studi di MilanoVia Celoria 220133 MilanoItaly Dipartimento di Scienze Agroalimentari Ambientali e AnimaliUniversitàDegli Studi di Udine33100 UdineItaly

This study investigated the effects of 2 Achillea moschata essential oils extracted from plants collected in 2 different valleys of the Italian Alps and 3 pure compounds of oils—bornyl acetate(BOR),camphor(CAM),and eucalyptol(EUCA)—on in vitro ruminal fermentation and *** in vitro batch fermentation experiment(Exp.1)tested the addition of all of the substances(2 essential oils and 3 compounds)in fermentation bottles(120 mL)at 48 h of incubation,whereas a subsequent in vitro continuous culture experiment(Exp.2)evaluated the pure compounds added to the fermenters(2 L)for a longer incubation period(9 d).In both experiments,total mixed rations were incubated with the additives,and samples without additives were included as the control(CTR).Each treatment was tested in duplicate and was repeated in 3 and 2 fermentation runs in Exp.1 and 2,*** production(GP)in Exp.1 was similar for all of the treatments,and short chain volatile fatty acid(SCFA)production was similar in both experiments except for a decrease of SCFA produced(P=0.029)due to EUCA addition in *** to CTR,BOR and CAM reduced the valerate proportion(P=0.04)in Exp.1,and increased(P<0.01)the acetate proportion in *** treatments increased(P<0.01)total protozoa counts(+36.7%and+48.4%compared to CTR on average for Exp.1 and 2,respectively).In Exp.1,all of the treatments lowered the Bacteroidetes and Firmicutes and increased the Proteobacteria relative abundances(P<0.05),whereas in Exp.2,the EUCA addition increased(P=0.012)the *** Exp.1,methane(CH4)as a proportion of the GP was lowered(P=0.004)by the addition of CAM and EUCA compared to CTR,whereas in Exp.2,EUCA reduced the amount of stoichiometrically calculated CH4 compared to ***,essential oils extracted from *** and the pure compounds did not depress in vitro rumen fermentation,except for EUCA in *** both experiments,an increase of the protozoal population occurred for all the additives.

关键词： Methane Rumen fermentation Achillea moschata Protozoa Microbiome

Fluoroquinolone-based protocols for eradication of Helicobacter pylori

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World Journal of Gastroenterology 2014年第27期20卷 8947-8956页

作者： Antonio Rispo pietro Capone fabiana Castiglione Luigi Pasquale Matilde Rea Nicola Caporaso Division of Gastroenterology Department of Clinical Medicine and Surgery-University "Federico Ⅱ" of Naples 80131 Napoli Italy Division of Gastroenterology P.O. "Sant’ Ottone Frangipane" Ariano Irpino 80131 Napoli Italy

Helicobacter pylori (H. pylori) is a widespread pathogen infecting about 40% of people living in urban areas and over 90% of people living in the developing regions of the world. H. pylori is well-documented as the main factor in the pathogenesis of peptic ulcer disease, chronic gastritis, and gastric malignancies such as cancer and mucosa-associated lymphoid tissue-lymphoma; hence, its eradication is strongly recommended. The Maastricht IV consensus, which focused on the management of H. pylori infection, set important new strategies in terms of treatment approaches, particularly with regards to first- and second-line treatment protocols and led to improved knowledge and understanding of H. pylori resistance to antibiotics. In recent years, various fluoroquinolone-based protocols, mainly including levofloxacin, have been proposed and effectively tested at all therapeutic lines for H. pylori eradication. The aim of the present paper is to review the scientific literature focused on the use of fluoroquinolones in eradicating H. pylori.

关键词： Helicobacter pylori eradication fluoroquinolone therapy

Comprehensive lumped parameter and multibody approach for the dynamic simulation of agricultural tractors with tyre–soil interaction

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IET Cyber-Systems and Robotics 2023年第3期5卷 1-23页

作者： Massimo Martelli Damiano Chiarabelli Silvia Gessi pietro Marani Emiliano Mucchi Marco Polastri Institute of Sciences and Technologies for Sustainable Energy and Mobility CNR‐STEMSNational Research CouncilFerraraItaly Department of Engineering University of FerraraFerraraItaly

Modern agricultural tractors are complex systems,in which multiple physical(and technological)domains interact to reach a wide set of competing goals,including work operational performance and energy *** complexity translates to the dynamic,multi‐domain simulation models implemented to serve as digital twins,for rapid prototyping and effective pre‐tuning,prior to bench and on‐field ***,a suitable simulation framework should have the capability to focus both on the vehicle as a whole and on individual *** each of the latter,multiple options should be available,with different levels of detail,to properly address the relevant phenomena,depending on the specific focus,for an optimal balance between accuracy and computation *** methodology proposed here by the authors is based on the lumped parameter approach and integrates the models for the following subsystems in a modular context:internal combustion engine,hydromechanical transmission,vehicle body,and tyre–soil *** model is completed by a load cycle module that generates stimulus time histories to reproduce the work load under real operating *** capability is affected by vertical load on the wheels,which is even more relevant if the vehicle is travelling on an uncompacted soil and subject to a variable drawbar pull force as it is when *** vertical load is,in turn,heavily affected by vehicle dynamics,which can be accurately modelled via a full multibody *** presented lumped parameter model is intended as a powerful simulation tool to evaluate tractor performance,both in terms of fuel consumption and traction dynamics,by considering the cascade phenomena from the wheel–ground interaction to the engine,passing through the dynamics of vehicle bodies and their mass *** capabilities and numerical results are presented for the simulation of a realistic ploughing operation.

关键词： agricultural tractor digital twin lumped parameter simulation,multibody powertrain tyre-soil interaction