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Phytochemical Composition and Insecticidal Property of Native Plants against the Cowpea Weevil Callosobruchus maculatus Fab. (Coleoptera: Bruchidae) for Natural Preservation of Cowpea Seeds

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Advances in Entomology 2024年第1期12卷 56-66页

作者： Elias Mano Rabièta Simde Koï Wenceslas Kam Nadjatou Ouédraogo Désiré Dramane Abdoul Khader Sanou Rasmané Abdou Ouédraogo Boubakar Ouattara pierre alexandre eric djifaby sombié Souleymane Nacro Laboratory of Natural Substances and Technologies of Natural Products and the Environment (LABTECH-PRONE) Institute of Research in Applied Sciences and Technologies (IRSAT) Ouagadougou Burkina Faso Central Horticulture Laboratory of the Regional Center of Excellence in Fruits and Vegetables of Bobo-Dioulasso Institute of Environment and Agricultural Research (INERA) Bobo Dioulasso Burkina Faso Department of Animal Biology and Physiology University Joseph Ki-Zerbo Ouagadougou Burkina Faso National Agricultural Training School of Matourkou (ENAFA) Bobo Dioulasso Burkina Faso Institute of Rural Development (IDR) Nazi Boni University Bobo Dioulasso Burkina Faso

About 80% of cowpea seeds are lost along with 30% of their weight after six months of unprotected storage due to attacks by Callosobruchus maculatus. This study aimed at evaluating the effect of applying bioinsecticides on the biological parameters of C. maculatus to reduce its damage to cowpea stocks. Four treatments including an untreated control and three doses of powdery extracts of C. nigricans, Z. zanthoxyloides and H. suaveolens, were compared in a Fischer block with four completely randomized repetitions. One hundred undamaged cowpea seeds were mixed with doses of each extract in Petri dishes and then fed as food substrates to ten pairs of C. maculatus for eight days. The extracts of H. suaveolens, C. nigricans and Z. zanthoxyloides all contain active ingredients including polyphenols, flavonoids, anthocyanins and alkaloids, etc. The extract of Z. zanthoxyloides was found to be more active on adults (100.00% mortality) followed by C. nigricans (98.75% mortality) and H. suaveolens (97.50% mortality) at 80 mg/g. Fecundity per female decreased significantly in the treated batches of 13.82 eggs for H. suaveolens at 10.12 eggs for C. nigricans at a dose of 80 mg/g. Germination rate of seeds was statistically raised to 100% at 60 and 80 mg/g by C. nigricans powder and lowered to 83.00% at 20 mg/g by Z. zanthoxyloides extracts. The application of Z. zanthoxyloides extract would be a less expensive alternative for the sustainable management of C. maculatus in cowpea stocks.

关键词： Cassia nigricans Hyptis suaveolens Zanthoxylum zanthoxyloides Biological Activities Vigna unguiculata Pest Burkina Faso

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The Effect of Seed Size on Phytochemical Composition in Cowpea Lines (<i>Vigna unguiculata</i>(L.) Walp.) from Burkina Faso

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Agricultural Sciences 2021年第12期12卷 1462-1472页

作者： pierre alexandre eric djifaby sombié Hemayoro Sama Antoine Barro Adama Hilou Martin Kiendrébéogo Institute of Environment and Agricultural Research Ouagadougou Burkina Faso Laboratory of Biochemistry and Chemistry Applied (LABIOCA) University of Ouagadougou Ouagadougou Burkina Faso University of Dedougou Dédougou Burkina Faso

The study aimed to determine the effect of seeds size (length, width, thickness and 100 seeds-weight) on their phytochemical composition (total phenolics, total flavonoids, total proteins, soluble sugars and starch) of cowpea accessions (Vigna unguiculata (L.) Walp.) grown in Burkina Faso. The genotypes and their descendants had length, width, thickness and 100 seeds weight between 80 ± 0.7 to 7.40 ± 0.46 cm;6.51 ± 0.41 to 5.76 ± 0.46 cm;5.15 ± 0.37 to 4.22 ± 0.22 cm;12.93 ± 0.09 to 20.16 ± 0.39 g respectively. Total phenol, total flavonoid and starch contents ranged from 585.37 ± 9.73 to 1704.71 ± 4.87 mg GAE per 100 g DW;41.42 ± 2.03 to 52.26 ± 0.75 mg QE per 100 g DW;6.45% ± 0.3% to 46.58% ± 0.12% respectively. The soluble sugars and total proteins contents were ranged respectively from 149.33 ± 3.80 to 432.82 ± 40.81 μg/100mg, and 20.28 ± 0.17 to 27.32 ± 0.17 (%). Seeds size characters had significant and positive correlations with starch content, and significant and negative correlations with total phenolic content. The study of the genetic parameters of phytochemical traits also revealed that they are slightly influenced by the environment. Genetic factors are believed to account for the variation in the observed seed traits, which provides a high potential of studied accessions for breeding programs.

关键词： Cowpea Seeds Size Phytochemistry Genetic Parameters

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Evaluation of Soybean Genotypes (Glycine max L. Merr.) Susceptibility to Parasitic Nematodes in Western Part of Burkina Faso

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Agricultural Sciences 2023年第1期14卷 23-34页

作者： Gilles Ibié Thio Bouma Thio Nofou Ouédraogo Frank Essem Inoussa Drabo Oumar Boro Fabrice Wendyam Nikiema pierre alexandre eric djifaby sombié David Yago Hervé Bépio Bama Mahamadou Sawadogo Paco Sérémé Institut de l’Environnement et de Recherches Agricoles (INERA) Ouagadougou Burkina Faso Akenten Appiah-Menka University of Skills Training and Entrepreneurial Development Ashanti Mampong Ghana Université Joseph KI-ZERBO/UFR Science de la Vie et de la Terre Ouagadougou Burkina Faso

Parasitic nematodes have been reported as one of the major constraints to soybean production worldwide. The majority of nematodes are so-called “free-living” and feed mainly on bacteria, fungi, protozoa and other nematodes. In Burkina Faso, the presence of parasitic nematodes has been reported in crops such as rice and sorghum. The objective of this study was to identify the genera and species of nematodes associated with soybean production in Burkina Faso. Investigations were carried out on 24 genotypes of a medium-maturity group of soybean at the Farako-Ba research station. Soil samples were taken from the trial soil before its installation. At harvest, soil samples with roots were taken from each genotype in the elementary plots. The composite sample is represented by 4 lots of soil samples with roots. Extractions and counts of nematodes were performed on the different lots. In total, 7 genera of plant-parasitic nematodes associated with soybean were identified. Among these genera, Pratylenchus (100% of infected genotypes), Helicotylenchus (97.28%) and Scutellonema (94.44%) were the most prevalent in terms of frequency and abundance. Some soybean genotypes were less susceptible to the genus Pratylenchus is known to be highly pathogenic in soybean. These were mainly the genotypes TGX2025-10E, TGX2023-3E and TGX2025-14E.

关键词： Parasitic Nematodes Susceptibility Genotypes Soybean Burkina Faso

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Comparison of Two Recurrent Neural Networks for Rainfall-Runoff Modeling in the Zou River Basin at Atchérigbé (Bénin)

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Journal of Geoscience and Environment Protection 2024年第9期12卷 167-181页

作者： Iboukoun Eliézer Biao Oscar Houessou pierre Jérôme Zohou Adéchina eric Alamou National School of Mathematical Engineering and Modeling National University of Sciences Technology Engineering and Mathematics Abomey Benin LaGEA National School of Public Works National University of Sciences Technology Engineering and Mathematics Abomey Benin International Chair of Mathematical Physics and Applications (ICMPA-UNESCO Chair) University of Abomey-Calavi Abomey-Calavi Bnin

Hydrological models are developed to simulate river flows over a watershed for many practical applications in the field of water resource management. The present paper compares the performance of two recurrent neural networks for rainfall-runoff modeling in the Zou River basin at Atchérigbé outlet. To this end, we used daily precipitation data over the period 1988-2010 as input of the models, such as the Long Short-Term Memory (LSTM) and Recurrent Gate Networks (GRU) to simulate river discharge in the study area. The investigated models give good results in calibration (R2 = 0.888, NSE = 0.886, and RMSE = 0.42 for LSTM;R2 = 0.9, NSE = 0.9 and RMSE = 0.397 for GRU) and in validation (R2 = 0.865, NSE = 0.851, and RMSE = 0.329 for LSTM;R2 = 0.9, NSE = 0.865 and RMSE = 0.301 for GRU). This good performance of LSTM and GRU models confirms the importance of models based on machine learning in modeling hydrological phenomena for better decision-making.

关键词： Supervised Learning Modeling Zou Basin Long and Short-Term Memory Gated Recurrent Unit Hyperparameters Optimization

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Diatom and Diatomite: Different Focus on Natural Media to Material Science Path

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American Journal of Analytical Chemistry 2024年第1期15卷 1-29页

作者： Secou Sall Vasiliki Papaefthimiou Thierry Dintzer Fabrice Vigneron eric Brendlé pierre Petit Tom Ferte Saliha Haddoum Sana Labidi Corinne Petit ICPEES UMR-CNRS 7515 Strasbourg University Strasbourg France Adscientis Wittelsheim France ICS CNRS UPR 22 Strasbourg France IPCMS UMR CNRS 7504 Strasbourg University Strasbourg France Ecole Nationale Polytechnique El-Harrach Algeria

Bio-silica issued from diatom, a microalgae, is attracted increasing attention in material science thanks to its peculiar nanoarchitecture and related properties with versatile applications. The present work is a deep analysis on morphological and chemical properties of bio-silica issued from fossil origin (diatomaceous earth) and living one (algal paste). An optimization in purification protocol was performed to obtain multiparous bio-silica from its raw media with keeping its original shape entirely. Multiple characterization methods as scanning electronic microscopy (SEM), infrared spectroscopy, x-ray diffraction (DRX), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDX), nitrogen adsorption and inverse gas chromatography (IGC), were used to check the purification protocol efficiency as well as to gather accurate information on morphology and chemical composition of diatom material obtained in large amount.

关键词： Diatom Diatomite Bio-Silica Purification Frustule

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Neonatal Intestinal Obstruction in Acute Renal Failure in Premature Infant: A Case Report

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Open Journal of Pediatrics 2024年第2期14卷 338-343页

作者： Ondima Lucienne Irène Patricia Okiemy Niendet Cardinale Princilia Gandzali-Ngabe pierre eric Caryne Mboutol-Mandavo Bosseba Missengui Rhodia Hélène Akobande Nuptia erica Department of Paediatric Surgery CHU Brazzaville Brazzaville Congo Faculty of Health Sciences Marien Ngouabi University Brazzaville Congo Department of Nephrology and Dialysis Brazzaville University Hospital Brazzaville Congo

Introduction: Intestinal obstruction is a common cause of abdominal surgery in the neonate. Diagnosis is straightforward using standard radiology, and surgical technique depends on the underlying anatomical lesion. Peritoneal dialysis (PD) is an effective, albeit invasive, therapy for neonatal renal failure. We report a case of neonatal obstruction with severe renal failure treated by PD to highlight our hospital practice and possible remedies in a context of limited resources. Case Presentation: This was a female neonate of moderate prematurity admitted on day 4 of life for management of a flat neonatal obstruction. Radiological diagnosis suggested small bowel atresia. Biological tests revealed severe renal failure with creatinine levels of 416 micromoles per liter and blood urea of 27.1 micromoles per liter. Management consisted of preoperative peritoneal dialysis for 48 hours followed by laparotomy. The intraoperative diagnosis was GROSFELD type IIIa digestive atresia. The postoperative course was favourable, transit was resumed on day 5 and the patient returned home on day 12. Progress at 3 months was satisfactory. Conclusion: Neonatal intestinal obstruction with renal failure in premature infants is associated with a poor prognosis, even more so if there is a delay in treatment. Peritoneal dialysis seems to be a suitable alternative for this management in our working conditions with limited resources.

关键词： Neonate Occlusion Peritoneal Dialysis Case Report

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Germinal center-dependent and-independent immune responses of tumor-infiltrating B cells in human cancers

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Cellular & Molecular Immunology 2023年第9期20卷 1040-1050页

作者： Eve Playoust Romain Remark eric Vivier pierre Milpied Marseille Université CNRSINSERMCentre d’Immunologie de Marseille-LuminyMarseilleFrance Innate Pharma MarseilleFrance

B cells play essential roles in immunity,mainly through the production of high affinity plasma cells(PCs)and memory B(Bmem)*** affinity maturation and differentiation of B cells rely on the integration of B-cell receptor(BCR)intrinsic and extrinsic signals provided by antigen binding and the microenvironment,*** recent years,tumor infiltrating B(TIL-B)cells and PCs(TIL-PCs)have been revealed as important players in antitumor responses in human cancers,but their interplay and dynamics remain largely *** lymphoid organs,B-cell responses involve both germinal center(GC)-dependent and GC-independent pathways for Bmem cell and PC *** maturation of BCR repertoires occurs in GC reactions with specific spatiotemporal dynamics of signal integration by B *** general,the reactivation of high-affinity Bmem cells by antigens triggers GC-independent production of large numbers of PC without BCR *** B-cell dynamics in immune responses requires the integration of multiple tools and readouts such as single-cell phenotyping and RNA-seq,in situ analyses,BCR repertoire analysis,BCR specificity and affinity assays,and functional ***,we review how those tools have recently been applied to study TIL-B cells and TIL-PC in different types of solid *** assessed the published evidence for different models of TIL-B-cell dynamics involving GC-dependent or GC-independent local responses and the resulting production of antigen-specific ***,we highlight the need for more integrative B-cell immunology studies to rationally investigate TIL-B cells as a leverage for antitumor therapies.

关键词： antibody tertiary lymphoid structure plasma cell germinal center cancer

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This work was supported by the European Research Council Advanced Grant(ETAP-742985 to *** J.F.)and by the Ministry of Science and Technology(grant 110-2636-B-005-001 to K.-J.L.).

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Plant Communications 2024年第1期5卷 130-147页

作者： Yu Zhang Yuan Fu Wenfei Xian Xiuli Li Yong Feng Fengjiao Bu Yan Shi Shiyu Chen Robin van Velzen Kai Battenberg Alison M.Berry Marco G.Salgado Hui Liu Tingshuang Yi Pascale Fournier Nicole Alloisio Petar Pujic Hasna Boubakri M.eric Schranz pierre-Marc Delaux Gane Ka-Shu Wong Valerie Hocher Sergio Svistoonoff Hassen Gherbi Ertao Wang Wouter Kohlen Luis G.Wall Martin Parniske Katharina Pawlowski Philippe Normand Jeffrey J.Doyle Shifeng Cheng Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518124China Biosystematics Group Department of Plant SciencesWageningen University6708PB Wageningenthe Netherlands Department of Plant Sciences University of CaliforniaDavisDavisCA 95616USA Department of Ecology Environment and Plant SciencesStockholm University10691 StockholmSweden Germplasm Bank of Wild Species Kunming Institute of BotanyChinese Academy of SciencesLanhei RoadKunming 650201China Universite´de Lyon Universite´Lyon 1CNRSUMR5557Ecologie MicrobienneINRAUMR 141843 bd du 11 novembre 191869622 VilleurbanneFrance Laboratoire de Recherche en Sciences Ve´ge´tales(LRSV) Universite´de ToulouseCNRSUPS24 chemin de Borde RougeAuzevilleBP4261731326 Castanet TolosanFrance Department of Biological Sciences University of AlbertaEdmontonAB T6G 2E9Canada French National Research Institute for Sustainable Development(IRD) UMR LSTM(IRD/CIRAD/INRAe/Montpellier University/Supagro)-Campus International BaillarguetTA A-82/J34398 Montpellier Cedex 5France National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and EcologySIBSChinese Academy of SciencesShanghaiChina Laboratory of Molecular Biology Department of Plant SciencesWageningen University6708PB Wageningenthe Netherlands Laboratory of Biochemistry Microbiology and Soil Biological InteractionsDepartment of Science and TechnologyNational University of QuilmesCONICETBernalArgentina Faculty of Biology GeneticsLMU MunichGroßhaderner Strasse 2-482152 MartinsriedGermany School of Integrative Plant Science Sections of Plant Biology and Plant Breeding&GeneticsCornell UniversityIthacaNY 14853USA State Key Laboratory of Systematic and Evolutionary Botany Institute of BotanyChinese Academy of SciencesBeijing 100093China Univer

Plant root-nodule symbiosis(RNS)with mutualistic nitrogen-fixing bacteria is restricted to a single clade of angiosperms,the Nitrogen-Fixing Nodulation Clade(NFNC),and is best understood in the legume *** species share many commonalities,explained either by divergence from a common ancestor over 100 million years ago or by convergence following independent origins over that same time ***,comparative analyses of diverse nodulation syndromes can provide insights into constraints on nodulation—what must be acquired or cannot be lost for a functional symbiosis—and the latitude for Plant Communications Genomic landscape of nodulation variation in the ***,much remains to be learned about nodulation,especially outside of ***,we employed a large-scale phylogenomic analysis across 88 species,complemented by 151 RNA-seq libraries,to elucidate the evolution of *** phylogenomic analyses further emphasize the uniqueness of the transcription factor NIN as a master regulator of nodulation and identify key muta-tions that affect its function across the *** transcriptomic assessment revealed nodule-specific upregulated genes across diverse nodulating plants,while also identifying nodule-specific and nitrogen-response *** 70%of symbiosis-related genes are highly conserved in the four representative species,whereas defense-related and host-range restriction genes tend to be lineage *** study also identified over 900000 conserved non-coding elements(CNEs),over 300000 of which are unique to sampled NFNC ***-specific CNEs are enriched with the active H3K9ac mark and are correlated with accessible chromatin regions,thus representing a pool of candidate regula-tory elements for genes involved in ***,our results provide novel insights into the evolution of nodulation and lay a foundation for engineering of RNS traits in agriculturally important crops.

关键词： nitrogen-fixing root-nodule symbiosis two competing hypotheses phylogenomics phylotranscrip-tomics conserved non-coding elements convergence deep homology

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Mapping data gaps to estimate biomass across Brazilian Amazon forests

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Forest Ecosystems 2020年第3期7卷 334-348页

作者： Graciela Tejada eric Bastos Görgens Alex Ovando Jean pierre Ometto Earth System Science Center(CCST) National Institute for Space Research(INPE)Av dos Astronautas 1758São Josédos CamposSP12227-010Brazil Department of Forestry Engineering Universidade Federal dos Vales do Jequitinhonha e MucuriCampus JKRod.MGT 367km 5835000Alto do JacubaDiamantinaMG39100-000Brazil National Center for Monitoring and Early Warning of Natural Disasters(CEMADEN) Estrada Doutor Alino Bondesani 500São Josédos CamposSP12247-016Brazil

Background: Tropical forests play a fundamental role in the provision of diverse ecosystem services, such as biodiversity,climate and air quality regulation, freshwater provision, carbon cycling, agricultural support and culture. To understand the role of forests in the carbon balance, aboveground biomass(AGB) estimates are needed. Given the importance of Brazilian tropical forests, there is an urgent need to improve AGB estimates to support the Brazilian commitments under the United Nations Framework Convention on Climate Change(UNFCCC). Many AGB maps and datasets exist, varying in availability, scale and coverage. Thus, stakeholders, policy makers and scientists must decide which AGB product, dataset or combination of data to use for their particular goals. In this study, we assessed the gaps in the spatial AGB data across the Brazilian Amazon forests not only to orient the decision makers about the data that are currently available but also to provide a guide for future ***: We obtained a map of the gaps in the forest AGB spatial data for the Brazilian Amazon using statistics and differences between AGB maps and a spatial multicriteria evaluation that considered the current AGB datasets. The AGB spatial data gap map represents areas with good coverage of AGB data and, consequently, the main gaps or priority areas where further biomass assessments should focus, including the northeast of Amazon State, Amapá and northeast of Pará. Additional y, by quantifying the variability in both the AGB maps and field data on multiple environmental factors,we provide valuable elements for understanding the current AGB data as a function of climate, soil, vegetation and ***: The map of AGB data gaps could become a useful tool for policy makers and different stakeholders working on National Communications, Reducing Emissions from Deforestation and Degradation(REDD+), or carbon emissions modeling to prioritize places to implement further AGB a

关键词： Amazon Tropical forest Carbon Aboveground biomass Data gaps REDD+ Environmental factors

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Comparative Genomic Study of the Thioredoxin Family in Photosynthetic Organisms with Emphasis on Populus trichocarpa

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Molecular Plant 2009年第2期2卷 308-322页

作者： Kamel Chibani Gunnar Wingsle Jean-pierre Jacquot eric Gelhaye Nicolas Rouhier UMR 1136 Nancy University-INRA Interactions Arbres Microorganismes IFR 110 GEEF Faculte des Sciences BP 239 54506 Vandoeuvre-les-Nancy Cedex France Department of Forest Genetics and Plant Physiology Swedish University of Agricultural Sciences Umea Plant Science Centre 90183 Umea Sweden

The recent genome sequencing of Populus trichocarpa and Vitis vinifera, two models of woody plants, of Sorghum bicolor, a model of monocot using C4 metabolism, and of the moss Physcomitrella patens, together with the availability of photosynthetic organism genomes allows performance of a comparative genomic study with organisms having different ways of life, reproduction modes, biological traits, and physiologies. Thioredoxins （Trxs） are small ubiq- uitous proteins involved in the reduction of disulfide bridges in a variety of target enzymes present in all sub-cellular compartments and involved in many biochemical reactions. The genes coding for these enzymes have been identified in these newly sequenced genomes and annotated. The gene content, organization and distribution were compared to other photosynthetic organisms, leading to a refined classification. This analysis revealed that higher plants and bryo- phytes have a more complex family compared to algae and cyanobacteria and to non-photosynthetic organisms, since poplar exhibits 49 genes coding for typical and atypical thioredoxins and thioredoxin reductases, namely one-third more than monocots such as Oryza sativa and S. bicolor. The higher number of Trxs in poplar is partially explained by gene duplication in the Trx m, h, and nucleoredoxin classes. Particular attention was paid to poplar genes with emphasis on Trx-like classes called Clot, thioredoxin-like, thioredoxins of the lilium type and nucleoredoxins, which were not described in depth in previous genomic studies.

关键词： Genomic oxidoreductase poplar redox regulation thioredoxins.

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