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Post Cryopreservation Growth Kinetic and Photosynthetic Assessment of an Acid Tolerant Strain of Stichococcus bacillaris

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Natural Resources 2024年第5期15卷 130-148页

作者： Marzia Licata Luigi Marra Nunzia Nappi Elena Aurino Feliciana Oliva Antonino De Natale Donato giovannelli Antonino Pollio Department of Biology University of Naples &#8220Federico II&#8221 Naples Italy ACUF Algal Collection of University of Naples &#8220Federico II&#8221 Naples Italy Department of Chemical Materials and Industrial Production Engineering University of Naples &#8220Federico II&#8221 P.le V. Tecchio Naples Italy Institute for Marine Biological Resources and Biotechnologies Italian National Research Council CNR-IRBIM Ancona Italy Earth-Life Science Institute ELSI Tokyo Institute of Technology Tokyo Japan Marine Chemistry and Geochemistry Department Woods Hole Oceanographic Institution Woods Hole MA USA Department of Marine and Coastal Science Rutgers University New Brunswick NJ USA

Preserving microbial diversity has become a strategic undertaking. Thus, ex situ microalgal culture conservation results in strategic and functional resource in both biodiversity protection and application domains. Cryopreservation of microalgae has been practiced since the 1960s and is now considered the optimal preservation strategy. Furthermore, the overall monitoring during growth of cultures after freezing/thawing protocols was hardly investigated and there is poor evaluation related to preserve especially the photosystem apparatus. The present study focuses on Stichococcus bacillaris as case study for short-term cryopreservation at −80 °C storage. Various freezing pretreatments using cryoprotective agents, and two thawing methods were compared introducing a novel variable to evaluate viability recovery and assessing growth kinetics of cultures immediately after thawing and after a series batch cultivation. Photosynthetic rate and pigments assessment were proposed to evaluate hidden metabolic cell damage. Results underline cryoprotective agents can increase the kinetic recovery of preserved cells in terms of reduction of lag phase during batch cultivation tests: the use of dimethyl sulfoxide and glycerol granted a growth comparable to unpreserved cells when sudden thawing occurs after 24 hours of storage, but recovery after preservation is less sensitive to cryoprotective agents when gradual thawing and 1 month of storage is considered. However, cells are always able to restore their physiological pathways even without agents, so their kinetic effect has been proved and quantified. Interestingly, both the photosynthetic efficiency and the ratio between total chlorophyll and carotenoids are comparable (0.75 Fv/Fm, 2.2 ± 0.25 g/g) to unpreserved cells and they are unsensitive to chosen agents, but the ratio between chlorophyll a and chlorophyll b was clearly altered (up to 10 times), suggesting that photoactive pigments relative proportions

关键词： Microalgae Stichococcus bacillaris Cryopreservation Growth Kinetic Photosynthetic Rate

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Low Rate of Oral Human Papilomavirus （Hpv） Infection in Women with Genital HPV Infection.

Low Rate of Oral Human Papilomavirus （Hpv） Infection in Wo...

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24th International Papillomavirus Conference and Clinical Workshop

作者： Lucia giovannelli Nicoletta Termine MariaColomba Migliore Giuseppina Campisi Giuseppina Capra Mariapia Caleca Pietro Ammatuna Universita di Palermo Universitadi Palermo Universitadi Palermo Universitadi Palermo Universitadi Palermo Universitadi Palermo Universitadi Palermo

＜正＞Infection with HPV of oral mucosa is currently recognized,but the figures on transmission of oral HPV infections are incom- pletely established To elucidate whether genital HPV infections are a risk factor for the infection of oral mucosa,this case- control study researched HPV presence in cytological scrapings of oral mucosa from 76 women with genital HPV infections

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经皮冠状动脉血管重建术时的血压水平与冠状动脉支架内再狭窄的风险

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中华高血压杂志 2016年第7期24卷 698-698页

作者： Tocci G Barbato E Coluccia R Modestino A Pagliaro B Mastromarino V giovannelli F Berni A Volpe M 陈云叶鹏

血压水平增高使进行过经皮冠状动脉介入治疗（percutaneous coronary interventions,PCI）的患者10年内心血管病和死亡率的风险处于很高危的水平。该研究目的是探讨患者PCI时血压水平对今后发生支架内再狭窄（in stent restenosis,ISR... 详细信息

血压水平增高使进行过经皮冠状动脉介入治疗（percutaneous coronary interventions,PCI）的患者10年内心血管病和死亡率的风险处于很高危的水平。该研究目的是探讨患者PCI时血压水平对今后发生支架内再狭窄（in stent restenosis,ISR）风险的作用。方法：回顾性分析行PCI的患者796例,

关键词：支架内再狭窄 restenosis stent 可逆性心肌缺血心血管病介入治疗药物洗脱支架 eluting 血管造影内直径

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