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Chinese Journal of Chemistry 2015年第9期33卷 1069-1076页

作者： Xiudong Yang Xue Li Ying Xu Zifeng pi Na Lin Zhiqiang Liu Fengrui Song National Center of Mass Spectrometry in Changchun & Jilin Province Key Laboratory of Chinese MedicineChemistry and Mass Spectrometry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun Jilin 130022 China Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences Beijing 100700 China

In traditional Chinese medicine theory, Panax ginseng and Veratrum nigrum L. is an important incompatible herb pair. Studies on the content variation of main components and the influences on the metabolism in rat intestinal bacteria are useful to understand the mechanism of incompatibility of this herb pairs. In this study, the content variation of ginsenosides and their metaboltic profiles in the extracts of P. ginseng and compatibility of P. ginseng with V. nigrum L. （G-V） were investigated using relative quantitative method of electrospray ionization mass spec- trometry （ESI-MS） and UPLC-MSn, respectively. The relative contents of most ginsenosides were reduced in the extract of G-V. Furthermore, ginsenosides Rbt, Rb2, Rc and Rd could be metabolized to Rd, F2 and C-K in rat in- testinal bacteria. The metabolic speeds ofRbl, Rb2 and re in the G-V extracts at ratios of 10 ： 5, 10 ： 7 and 10 ： 10 and the metabolic rates of ginsenosides Rbb Rb2 and Rc to Rd, Rd to F2 in all compatibility extracts were lower than that in the P ginseng extract. In conclusion, this study illustrated the mechanism of effect-reducing by comparison of the relative contents and metabolic profiles of ginsenosides after compatibility of P ginseng and V. nigrum L.

关键词： Panax ginseng Veratrum nigrum L. rat intestinal bacteria incompatibility UPLC-MS

Evaluation of Tear Malate dehydrogenase 2 in Mild Dry Eye Disease

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Eye Science 2014年第4期29卷 204-208页

作者： Qing Guo Houbin Huang Yuli pi Hancheng Zhang department of Ophthalmology First Hospital Affiliated to Chinese PLA General Hospital department of Ophthalmology Chinese PLA General Hospital department of Ophthalmology Zhujiang Hospital Southern Medical University

Purpose: To evaluate the effect of tear malate dehydrogenase2 on monitoring ocular surface injury in mild dry eye(de)***: A total of 15 de patients(30 eyes) with mild subjective symptoms but no ocular surface fluorescein staining signs were enrolled in this study.(de group)..The control group was 15 healthy age- and sex-matched volunteers(30eyes)..All subjects were asked to fill out a de symptoms questionnaire and take different tests including tear MDH and MDH2 activities evaluation,..tear breakup time.(TBUT),Schirmer I,.and slit-lamp examination of the ocular *** investigated different changes in tear MDH and MDH2 activities in the de group and control group,.discussed the association between tear MDH2 activity and de symptoms, and the relationship between tear MDH2 activity and diagnostic tests(Schirmer I and TBUT). We also analyzed the changes in tear MDH2 activities after the treatment with artificial ***:.Tear MDH activities in the de group and control group were 288 ±102 U / L and 259 ±112 U / L,.respectively,and this difference was not statistically significant(P >0.05).The tear MDH2 activities in de group were significantly increased compared with control group. Tear MDH2 was significantly and negatively correlated with the Schirmer's value(r=-0.733,Prrelation with soreness symptoms(r=0.687, P reatment with artificial tears relieved or eliminated all discomfort symptoms, together with a considerable decrease in MDH2 activities(P re ***: Tear MDH2 activity can indicate ocular surfaceinjury in mild de patients and may be used to monitor the response to therapy.

关键词：苹果酸脱氢酶撕裂评价眼病诊断测试疾病监测表面损伤对照组

Intersected functional zone of transcriptional regulators patterns stemness within stem cell niche of root apical meristem

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Journal of Integrative Plant Biology 2020年第7期62卷 897-911页

作者： Meizhi Xu Xu Gu Nengsong Liang Xinxin Bian Hong Wang Yaxin Qin Limin pi Shuang Wu College of Life Sciences FAFU-UCR Joint Center and Fujian Provincial Key Laboratory of Haixia Applied Plant Systems BiologyFujian Agriculture and Forestry UniversityFuzhou 350002China College of resource and Environment Fujian Agriculture and Forestry UniversityFuzhou 350002China Institute of Advanced studies Wuhan UniversityWuhan 430072China

Root stem cell niche(SCN)consists of a quiescent center(QC)and surrounding stem *** symplastic communication leads to loss of stemness in the whole *** SCN regulators were reported to move between cells for SCN ***,single mutant of these regulators is insu?cient to abolish QC stemness despite the high differentiation rate in surrounding stem *** dis-sect the mechanism behind such distinct stemness in SCN,we combined the mis-expression strategy with pWOX5:icals3m system in which QC is symplastically *** found the starch accumulation in QC could be synergistically repressed by WUSCHEL-re-LATED HOMEOBOX 5(WOX5),SHORT-ROOT(SHR),SCARCROW(SCR),and PLETHORA(PLT).Like PLTs,other core regulators also exhibited dimorphicfunctions by inhibiting differentiation at a higher dose while promoting cell division at a low protein *** located in the center of the intersected ex-pression zones,QC cells receive the highest level of core regulators,forming the most robust stemness within ***-reLATED HOMEOBOX 5 was su?cient to activate PLT1/2 expression,contributing to the QC-enriched *** results provide ex-perimental evidence supporting the long-standing hypothesis that the combination of spatial ex-pression,synergistic function and dosage effect of core regulators result in spatially distinct stemness in SCN.

关键词： dosage synergistic stem

Induced current of high temperature superconducting loops by combination of exciting coil and thermal switch

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Chinese Physics B 2022年第3期31卷 521-526页

作者： Jia-Wen Wang Yin-Shun Wang Hua Chai Ling-Feng Zhu Wei pi State Key Laboratory of New Energy renewable Power System North China Electric Power UniversityBeijing 102206China

With its commercialization, the second-generation(2G) high temperature superconducting(HTS) re–Ba–Cu–O(reBCO, re is rare earth) tape is extensively applied to the superconducting magnets in the high magnetic fields. However,unlike low temperature superconducting(LTS) magnets, the HTS magnet cannot operate in the persistent current mode(PCM) due to the immature superconducting soldering technique. In this paper, an exciting method for two HTS sub-loops,so-called charging and load loops, is proposed by flux pump consisting of exciting coil and controllable thermal *** HTS sub-loops are made of an reBCO tape with two slits. An exciting coil with iron core is located in one sub-loop and is supplied with a triangular waveform current so that magnetic field is generated in another sub-loop. The influence of magnetic flux on induced current in load loop is presented and verified in experiment at 77 K. The relationship between the induced magnetic flux density and the current on the sub-loops having been calibrated, magnetic flux density, and induced current are obtained. The results show that the HTS sub-loops can be excited by a coil with thermal switch and the induced current increases with magnetic flux of exciting coil increasing, which is promising for persistent current operation mode of HTS magnets.

关键词： bridge high temperature superconducting(HTS)sub-loops induced current thermal switch

Analysis of the Self-Protection Characteristics of a 1.5T Bitter-Like HTS Magnet Operated at 65K

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Chinese Physics Letters 2018年第6期35卷 79-83页

作者： Yan-Bing Hou Yin-Shun Wang Chang-Tao Kan Xi Yuan Wei pi State Key Laboratory of New Energy Power System North China Electric Power University

We present a conceptual configuration of a high-temperature superconducting（HTS）magnet made from reBCO（re=Rare Earth,B=Barium,C=Copper,O=Oxide）annular plates,called a Bitter-like HTS magnet,which can operate in persistent current mode without joint resistance and can be excited by a flux pump and without current leads and a persistent power *** reBCO annular magnet which can generate 1.5 T corresponding to the operating current density 80%of critical current density of the magnet at an operating temperature of65 K is conceptually *** the thermal stability of the magnet is numerically simulated by Comsol *** a piece of RBCO annular plate quenches,the maximum released energy is its stored energy because each reBCO annular plate in the Bitter-like magnet is in *** calculate the stored energy in the reBCO annular plate,the inductance of every annular plate,including self-inductance and mutual inductance,is *** with the minimum quench energy（MQE）and stored energy in one reBCO annular plate,the stored energy in one reBCO annular plate is always smaller than the MQE,and the reBCO annular plate will not be damaged even though the stored energy in the reBCO annular plate is fully released,which indicates that this 1.5 T Bitter-like magnet has the property of self-protection.

关键词： reBCO Analysis of the Self-Protection Characteristics of a 1.5T Bitter-Like HTS Magnet Operated at 65K HTS

Plant Phosphatidylcholine-Hydrolyzing Phospholipases C NPC3 and NPC4 with Roles in Root development and Brassinolide Signaling in Arabidopsis thaliana

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Molecular Plant 2010年第3期3卷 610-625页

作者： Rinukshi Wimalasekera Premysl Pejchar Andre Holka Jan Martinec Gunther re. Scherer Leibniz University of Hannover Institute of Floriculture and Wood Science Section of Applied Molecular Physiology Herrenhauser Strasse 2 D-30419Hannover Germany Institute of Experimental Botany Academy of Sciences of the Czech Republic v.v.i. Rozvojova 263 165 02 Prague 6 Czech Republic

Phosphatidylcholine-hydrolyzing phospholipase C （PC-PLC） catalyzes the hydrolysis of phosphatidylcholine （PC） to generate phosphocholine and diacylglycerol （DAG）. PC-PLC has a long tradition in animal signal transduction to generate DAG as a second messenger besides the classical phosphatidylinositol splitting phospholipase C （pi-PLC）. Based on amino acid sequence similarity to bacterial PC-PLC, six putative PC-PLC genes （NPC1 to NPC6） were identified in the Arabidopsis genome. RT-PCR analysis revealed overlapping expression pattern of NPC genes in root, stem, leaf, flower, and silique. In auxin-treated PNPc3：GUS and PNPc4：GUS seedlings, strong increase of GUS activity was visible in roots, leaves, and shoots and, to a weaker extent, in brassinolide-treated （BL） seedlings. PNPc4：GUS seedlings also responded to cytokinin with increased GUS activity in young leaves. Compared to wild-type, T-DNA insertional knockouts npc3 and npc4 showed shorter primary roots and lower lateral root density at low BL concentrations but increased lateral root densities in response to exogenous 0.05-1.0 I^M BL BL-induced expression of TCH4 and LRX2, which are involved in cell expansion, was impaired but not impaired in repression of CPD, a BL biosynthesis gene, in BL-treated npc3 and npc4. These observations suggest NPC3 and NPC4 are important in BL-mediated signaling in root growth. When treated with 0.1 I^M BL, DAG accumulation was observed in tobacco BY-2 cell cultures labeled with fluorescent PC as early as 15 min after application. We hypothesize that at least one PC-PLC is a plant signaling enzyme in BL signal transduction and, as shown earlier, in elicitor signal transduction.

关键词： Auxin brassinolide signaling phosphate deficiency phosphatidylcholine-splitting phospholipase C (PC-PLC) NPC genes.

Investigation of Aconitine-type Alkaloids from Processed Tuber of Aconitum carmiechaeli by HPLC-ESI-MS/MS^n

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Chemical research in Chinese Universities 2007年第5期23卷 625-627页

作者： YUE Hao pi Zi-feng ZHAO Yu-feng SONG Feng-rui LIU Zhi-qiang LIU Shu-ying Changchun Center of Mass Spectrometry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 P. R. China Graduate School of the Chinese Academy of Sciences Beijing 100039 P. R. China

Introduction Aconitine-type alkaloids isolated from the roots of Aconitum carmiechaeli show a potential toxicity and a broad spectrum of bioactivity[1-4].On the basis of the C8-substituent of C19-diterpenoid skeleton,aconitine-type alkaloids can be divided into diester-diterpenoid alkaloids（DDAs）,monoester-diterpenoid alkaloids（MDAs）,and lipo-alkaloids（Fig.1）.

关键词： Aconitine-type alkaloids HPLC-ESI-MS/MS^n Aconitum carmiechaeli

Polydopamine-modified chitin conduits with sustained release of bioactive peptides enhance peripheral nerve regeneration in rats

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Neural regeneration research 2022年第11期17卷 2544-2550页

作者： Ci Li Song-Yang Liu Li-ping Zhou Tian-Tian Min Meng Zhang Wei pi Yong-Qiang Wen Pei-Xun Zhang department of Orthopedics and Trauma Peking University People’s HospitalBeijingChina Key Laboratory of Trauma and Neural regeneration Ministry of EducationBeijingChina National Center for Trauma Medicine BeijingChina Beijing Key Laboratory for Bioengineering and Sensing Technology School of Chemistry and Biological EngineeringUniversity of Science and Technology BeijingBeijingChina

The introduction of neurotrophic factors into injured peripheral nerve sites is beneficial to peripheral nerve ***,neurotrophic facto rs are rapidly degraded in vivo and obstruct axonal regeneration when used at a supraphysiological dose,which limits their clinical *** mimetic peptides have been developed to be used in place of neurotrophic factors because they have a similar mode of action to the original growth fa ctors and can activate the equivalent receptors but have simplified sequences and *** this study,we created polydopamine-modified chitin conduits loaded with brain-derived neurotrophic factor mimetic peptides and vascular endothelial growth fa ctor mimetic peptides(Chi/PDA-Ps).We found that the Chi/PDA-Ps conduits were less cytotoxic in vitro than chitin conduits alone and provided sustained release of functional *** this study,we evaluated the biocompatibility of the Chi/P DA-Ps ***-derived neurotrophic factor mimetic peptide and vascular endothelial growth fa ctor mimetic peptide synergistically promoted prolife ration of Schwann cells and secretion of neurotrophic factors by Schwann cells and attachment and migration of endothelial cells in *** Chi/P DA-Ps conduits were used to bridge a 2 mm gap between the nerve stumps in rat models of sciatic nerve *** found that the application of Chi/PDA-Ps conduits could improve the motor function of rats and reduce gastrocnemius *** electrophysiological results and the microstructure of regenerative nerves showed that the nerve conduction function and re myelination was further resto *** findings suggest that the Chi/PDA-Ps conduits have great potential in peripheral nerve injury repair.

关键词： angiogenesis bioactive peptides nerve repair neurotrophic factor peripheral nerve injury peripheral nerve regeneration polydopamine surface modification synergistic effects tissue engineering

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老年群体数字化生存能力研究

老龄化研究 2024年第3期11卷 584-590页

作者：贺静雯梅妍妍皮道坤张燨文张雪伍江苏理工学院经济学院江苏常州

随着信息技术在社会生活应用逐渐泛化,对人们的数字化能力要求逐渐提高。老年群体由于专业知识缺乏和数字化能力不足,融入数字化社会生活困难重重,不仅影响他们生活质量,还可能引发一系列的社会问题。研究受教育水平、收入水平、家庭数... 详细信息

随着信息技术在社会生活应用逐渐泛化,对人们的数字化能力要求逐渐提高。老年群体由于专业知识缺乏和数字化能力不足,融入数字化社会生活困难重重,不仅影响他们生活质量,还可能引发一系列的社会问题。研究受教育水平、收入水平、家庭数字反哺水平以及社交水平四个因素对数字化生存能力的影响,开展影响机制分析,并运用多元线性回归分析进行实证研究。研究结果表明,老年群体数字化生存能力较低,较高的受教育水平、收入水平、家庭数字反哺水平以及社交水平会显著促进老年群体数字生存能力的提高。应采取加大适老化产品研发奖励力度、提升老年群体数字素养和能力、保障老年群体信息安全、家庭数字反哺和强化社交工具适老化改造等举措,推动老年群体顺利融入数字社会。

关键词：老年群体数字社会数字化生存能力适老化改造

THROMBOENDARTEreCTOMY FOR CHRONIC PULMONARY THROMBOEMBOLISM

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Chinese Medical Sciences Journal 2005年第3期20卷 194-197页

作者： Hua ren pi-xiong Su Chao-ji Zhang Song Gu Heng Zhang Chen Wang department of Cardiovascular Surgery Peking Union Medical College Hospital Chinese Academy of Medical Sciences ＆ Peking Union Medical College Beijing 100730 department of Cardiovascular Surgery Chaoyang Hospital Beijing 100007 department of respiratory Medicine Chaoyang Hospital Beijing 100007

Objective To evaluate the improving reliability and safety of thromboendarterectomy and perioperative management for chronic pulmonary thromboembolism. Methods The clinical data of 12 cases with chronic pulmonary thromboembolism, who underwent thromboendarterec- tomy assisted by low flow or circulation arrest with deep hypothermia, were reviewed retrospectively. results Pulmonary artery pressure decreased 20 to 40 mmHg immediately after surgical procedures in 9 cases. The postoperative pulmonary edema at various degrees happened in 12 cases, among them, 1 died of severe lung infection and pulmonary re-embolism at 19 days postoperation. Computed tomography pulmonary angiography and angiography of 11 cases indicated that the original obstruction of pulmonary artery disappeared. During the follow-up period of 2 months to 5 years, the clinical symptoms and activity was improved. Conclusion Thromboendarterectomy is an effective treatment for chronic pulmonary thromboembolism. The outcome of the surgical procedure needs to be further investigated and followed up regularly according to an evaluative system, because it might be influenced by multiple factors.

关键词： chronic pulmonary thromboembolism .thromboendarterectomy pulmonary hypertension postoperative pulmonary edema