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Species of Lasiodiplodia associated with mango in Brazil

Fungal Diversity 2013年第4期61卷 181-193页

作者： Marília W.Marques nelson B.Lima Marcos Antônio de Morais Jr Maria Angélica G.Barbosa Breno O.Souza Sami J.Michereff Alan J.L.phillips Marcos P.S.Câmara Departamento de Agronomia Universidade Federal Rural de Pernambuco52171-900 RecifeBrazil Departamento de Genética Universidade Federal de Pernambuco50732-970 RecifeBrazil Embrapa Semi-Árido 56302-970 PetrolinaBrazil Centro de Recursos Microbiológicos Departamento de Ciências da VidaFaculdade de Ciências e TecnologiaUniversidade Nova de Lisboa2829-516 CaparicaPortugal

Mango(Mangifera indica)is a major tropical fruit species cultivated in *** objective of this study was to identify species of Lasiodiplodia associated with dieback and stem-end rot of mango in the semi-arid region of northeastern Brazil,and compare the species in relation to mycelial growth,pathogenicity and virulence.A total of 120 isolates of Lasiodiplodia were used and identifications were made using a combination of morphology and phylogenetic analysis based on partial translation elongation factor 1-αsequence(EF1-α)and internal transcribed spacers(ITS).The following species were identified:Lasiodiplodia crassispora,***,***,***,***,*** and Lasiodiplodia sp..Lasiodiplodia theobromae was the most frequently isolated species,which represented 41%of all the *** this species had been previously reported on mango in Brazil,while the other species represent the first report associated with mango tree diseases in this *** crassispora is reported for the first time associated with mango diseases *** were significant differences in mycelial growth rates among the Lasiodiplodia species and also in the optimum temperature for *** species of Lasiodiplodia were pathogenic on mango *** were significant differences in virulence among the species,wherein *** and Lasiodiplodia *** the most virulent,while the least virulent were ***,***,*** and ***.

关键词： Botryosphaeriaceae Mangifera indica ITS EF1-α Phylogeny Virulence

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曼氏血吸虫病抗独特型抗体制备初报

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中国兽医寄生虫病 1994年第2期2卷 1-4页

作者：林矫矫 S．M．phillips 中国农科院上海家畜寄生虫病研究所美国宾夕法尼亚大学医学院

用曼氏血吸虫抗原特异性的保护性单抗Ｄ６多次免疫新西兰大白兔，从１只免疫兔分离得到的免疫血清在ＥＬＩＳＡ试验中对单抗Ｄ６的反应，比对血吸虫的其它两株同亚型抗原特异性单抗Ｎ（１２２）和Ｎ（３４）的反应强，能强烈地抑制单抗... 详细信息

用曼氏血吸虫抗原特异性的保护性单抗Ｄ６多次免疫新西兰大白兔，从１只免疫兔分离得到的免疫血清在ＥＬＩＳＡ试验中对单抗Ｄ６的反应，比对血吸虫的其它两株同亚型抗原特异性单抗Ｎ（１２２）和Ｎ（３４）的反应强，能强烈地抑制单抗Ｄ６识别曼氏血吸虫尾蚴抗原（ＣＡＰ）和虫卵抗原（ＳＥＡ），而不能抑制单抗Ｎ（１２２）和Ｎ（３４）识别ＣＡＰ和ＳＥＡ。多次实验的结果还证明，单抗Ｄ６免疫兔血清不能识别ＣＡＰ和ＳＥＡ，正常兔血清不能抑制单抗Ｄ６识别ＣＡＰ和ＳＥＡ。单抗Ｄ６免疫兔血清经饱和硫酸铵和结合有正常小鼠血清蛋白的免疫亲和层析柱纯化后，其免疫原性不变。这些结果说明，免疫兔血清中含有抗保护性单抗Ｄ６的抗独特型抗体。日后可用它免疫动物，探索抗独特型抗体疫苗在血吸虫病免疫预防上的应用价值。

关键词：单抗抗独特型抗体血清 CAP 曼氏血吸虫病抗原特异性抑制初报抗n 识别

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离体培养种胚使红三叶与多年生三叶草种杂交成功

离体培养种胚使红三叶与多年生三叶草种杂交成功

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“加强世界饲草——牲畜系统”第十四届国际草地会议

作者： G.B.COLLInS n.L.TAYLOR G.C.phillips 吴永敷 University of Kentucky Lexingtonky.U.S.A.

该项研究的目的是用长寿命的多年生三叶草种与农艺性状合乎要求的红三叶种杂交。以前许多人想以常规的方法来进行红三叶与多年生三叶草种的杂交,但都未成功。在最近的研究中,种胚离体培养方法已用来促进败育前的杂种种胚的生长和恢复。...

该项研究的目的是用长寿命的多年生三叶草种与农艺性状合乎要求的红三叶种杂交。以前许多人想以常规的方法来进行红三叶与多年生三叶草种的杂交,但都未成功。在最近的研究中,种胚离体培养方法已用来促进败育前的杂种种胚的生长和恢复。这一较大的成果是一条有助于*** Hazsl与红三叶杂交的捷径。在间三叶、*** L三叶草与红三叶草的杂交中也取得了初步的效果。用多年生草种做母本进行杂交。杂交后从授粉12—19天的小花中,用无菌操作法将未成熟的,呈心脏形的种胚解剖下来,培养在球脂凝胶培养基上,该培养基是从以前配制的适合于红三叶组织和细胞培养的培养基改良而来的,最初的培养周期是在高浓度蔗糖培养基上培养8—14天,此培养基含中等含量的植物生长素,低含量的细胞激动素。高含量蔗糖防止了种胚过早产生胚根。而所用生长调节剂的含量要有利于种胚的成熟。培养试验成活的种胚要转移到低含量生长素和中等含量的细胞激动素的培养基上培养,此培养基有助于幼苗的发芽。在低含量生长素,高含量细胞激动素的培养基上培养能增加杂种幼苗的数目,而且在幼苗转移到温室土壤之前有利于生根。从*** X红三叶的杂交中已获得许多杂种植株。在适宜的培养顺序下,大约有10%的培养种胚使其恢复了活性。使杂种的五个F株系成功地种植到了成熟。根据细胞学与遗传学的特征来检查被离体培养的植株证明确是杂种。用同样的方法,间三叶,***三叶草与红三叶的杂交已得到一些植株。但是否为杂种还未找到证据。在这些研究中所采用的种胚离体培养方法已获得初步的成功,证实了多年生三叶草种与红三叶杂交的可能性。这些方法应该用于产生红三叶的其他种间杂种上。通过种间杂种把多年生性的基因转移到红三叶上,这将会由于改良了红三叶的田间持久性,而在生产上具有巨大的价值。

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Outline of Ascomycota: 2017

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Fungal Diversity 2018年第1期88卷 167-263页

作者： nalin n.Wijayawardene Kevin D.Hyde H.Thorsten Lumbsch Jian Kui Liu Sajeewa S.n.Maharachchikumbura Anusha H.Ekanayaka Qing Tian Rungtiwa Phookamsak Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of ScienceKunming 650201YunnanPeople’s Republic of China Science&Education The Field Museum1400 S.Lake Shore DriveChicagoIL 60605USA Guizhou Institute of Biotechnology Guizhou Academy of Agricultural SciencesGuiyang 550006People’s Republic of China Department of Crop Sciences College of Agricultural and Marine SciencesSultan Qaboos UniversityPO Box 34123 AlkhoudOman Center of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand

Taxonomic placement of genera have been changing rapidly as taxonomists widely use DnA sequence data in phylogenetic and evolutionary studies. It is essential to update existing databases/outlines based on recent studies, since thesesources are widely used as a foundation for other research. In this outline, we merge both asexual and sexual genera intoone outline. The phylum Ascomycota comprises of three subphyla viz. Pezizomycotina (including 13 classes, 124 ordersand 507 families), Saccharomycotina (including one class, one order and 13 families) and Taphrinomycotina (five classes,five orders and six families). Approximately, 6600 genera have been listed under different taxonomic ranks includingauxiliary (intermediate) taxonomic ranks.

关键词： Asexual genera Classification Sexual genera Systematic Taxonomic ranks

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Three-Photon Up-conversion from Polymeric Lanthanide (III) Complexes

Three-Photon Up-conversion from Polymeric Lanthanide (III) C...

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第五届全国稀土发光材料学术研讨会

作者： Ka-Leung Wong Wai-Ming Kwok Wing-Tak Wong David Lee phillips Kok-Wai Cheah Department of Chemistry The University of Hong Kong Pokfulam Road Hong Kong P. R.China Department of Physics Hong Kong Baptist University Kowloon Tong Hong Kong P. R.China

We report the first observation of three-photon up-conversion emission from polymeric organic lanthanide complexes, [n,n’,n”-tris(2–methoxybenzamido)-triethylamine]-lanthanide complexes (Ln = Tb, Eu),

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Forecasting the number of species of asexually reproducing fungi(Ascomycota and Basidiomycota)

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Fungal Diversity 2022年第3期114卷 463-490页

作者： nalin n.Wijayawardene Alan J.L.phillips Diana Santos Pereira Dong-Qin Dai AndréAptroot Josiane S.Monteiro Irina S.Druzhinina Feng Cai Xinlei Fan Laura Selbmann Claudia Coleine Rafael F.Castañeda-Ruiz Martin Kukwa Adam Flakus Patricia Oliveira Fiuza Paul M.Kirk Kunhiraman C.Rajesh Kumar Ilesha S.leperuma Arachchi nakarin Suwannarach Li-Zhou Tang Teun Boekhout Chen Shuhui Tan R.P.Prabath K.Jayasinghe Marco Thines Centre for Yunnan Plateau Biological Resources Protection and Utilization College of Biological Resource and Food EngineeringQujing Normal UniversityQujing 655011YunnanPeople’s Republic of China Section of Genetics Institute for Research and Development in Health and Social CareNo:393/3Lily AvenueOff Robert Gunawardane MawathaBattaramulla 10120Sri Lanka Faculdade de Ciências Biosystems and Integrative Sciences Institute(BioISI)Universidade de LisboaCampo Grande1749-016 LisbonPortugal Laboratório de Botânica/Liquenologia Instituto de BiociênciasUniversidade Federal de Mato Grosso do SulAvenida Costa e Silva S/NBairro UniversitárioCampo GrandeMS CEP 79070-900Brazil Coordination of Botany Museu Paraense Emílio GoeldiBelémPA 66077830Brazil Fungal Genomics Laboratory(FungiG) College of Resources and Environmental SciencesNanjing Agricultural UniversityWeigang 1Nanjing 210095JiangsuPeople’s Republic of China The Key Laboratory for Silviculture and Conservation of Ministry of Education Beijing Forestry UniversityBeijing 100083People’s Republic of China Department of Ecological and Biological Sciences University of TusciaViterboItaly Italian national Antarctic Museum(MnA) Mycological SectionGenoaItaly Alejandro de Humboldt’(InIFAT) Instituto de Investigaciones Fundamentales en AgriculturaTropicalSantiago de Las VegasHavanaCuba Department of Plant Taxonomy and nature Conservation Faculty of BiologyUniversity of GdańskWita Stwosza 5980-308 GdańskPoland W.Szafer Institute of Botany Polish Academy of SciencesLubicz 4631-512 KrakowPoland Universidade Federal do Rio Grande do norte(UFRn) Programa de Pós-Graduação em Sistemática e EvoluçãoCentro de BiociênciasCampus UniversitárioAv.Senador Salgado Filho3000Lagoa NovaNatalRN 59078-970Brazil Biodiversity Informatics and Spatial Analysis Jodrell LaboratoryRoyal Botanic GardensKewRichmond TW93DSSurreyUK national Fungal Culture Collection of India(nFCCI) Biodiversity and Palaeobiology GroupMACS Agharkar Research InstituteG.G.

Asexually reproducing fungi play a significant role in essential processes in managed and wild ecosystems such as nutrients cycling and multitrophic interactions.A large number of such taxa are among the most notorious plant and animal *** addition,they have a key role in food production,biotechnology and *** without or rare sexual reproduction are distinguished based on their sporulating structures and conidiomata in traditional morphology-based *** number,variation and diversity of asexually reproducing taxa are insufficiently known,even though fungi capable of asexual reproduction may provide an untapped,rich biological resource for future ***,ca.30,000 asexual species belonging to ca.3800 genera have been reported(including 1388 coelomycetous and 2265 hyphomycetous genera).Recent reports(2017–2020)reiterate that the number of asexually producing fungi is higher than the number of frequently sexually-reproducing *** the advent of molecular tools and the abandonment of the dual nomenclature system for pleomorphic fungi,priority criteria were established and revisited in the latest outline of fungi and fungus-like ***,species numbers and taxonomic boundaries of pleomorphic taxa and their synanamorphs or synasexual morphs have yet to be *** number of species of speciose genera(***,Aspergillus,Cercospora,Fusarium,Phoma and Pseudocercospora),cryptic species,species of pleomorphic genera,less studied life modes(such as lichenicolous taxa,taxa from extreme environments)and species from biodiversity-rich areas still need evaluation to achieve more reliable estimates of their *** paper discusses the current knowledge on the matter,with diversity estimates,and potential obstacles in several chapters on(1)speciose genera;(2)pleomorphic genera;(3)cryptic species;(4)well-studied but insufficiently resolved taxa,*** inhabiting species,marine fungi,(5)less studied life modes,e.g.

关键词： Cryptic species DnA sequences Morphology Pleomorphism Speciose genera Species identificat

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阿基米德,数值分析学者

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数学通报 1985年第3期 42-44页

作者： G.M.phillips ,王连芬北京大学

1.引言设pn和Pn分别为单位圆的内接及外切正n边形的周长的一半。那么有p3=3（3～1/2）/2,P3=3（3～1/2）,p4=2（2～1/2）,P4=4。从几何上看,显然序列{Pn}及{Pn}分别是单调上升及单调下降序列,具有共同极限π。这就是阿基米德求π的近似... 详细信息

1.引言设pn和Pn分别为单位圆的内接及外切正n边形的周长的一半。那么有p3=3（3～1/2）/2,P3=3（3～1/2）,p4=2（2～1/2）,P4=4。从几何上看,显然序列{Pn}及{Pn}分别是单调上升及单调下降序列,具有共同极限π。这就是阿基米德求π的近似值的基本方法（可参看Heath[2].）阿基米德利用初等几何的推理方法,得到了下面的递推关系,在此关系式中这两个序列是交错地联

关键词：阿基米德递推关系外推法近似值相对误差数值分析

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单眼视减缓了青少年单侧近视的进展

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世界核心医学期刊文摘（眼科学分册） 2005年第12期1卷 40-41页

作者： phillips J.R 王海燕 Department of Optometry and Vision Science University of Auckland Private Bag 92019 Auckland New Zealand.Dr

Aim: To evaluate the acceptability, effectivity, and side effects of amonovision spectacle correction designed to reduce accommodation and myopia progression in schoolchildren. Methods: Dominant eyes of 11 year old children with myopia (- 1.00 to - 3.00 D mean spherical equivalent)were corrected for distance; fellow eyes were uncorrected or corrected to keep the refractive imbalance n was followed with cycloplegic autorefraction and A-scan ultrasonography measures of vitreous chamber depth (VCD) for up to 30 months. Dynamic retinoscopy was used to assess accommodationwhile reading. Results: All children accommodated to read with the distance corrected (dominant) eye. Thus, the near corrected eye experienced myopic defocus at all levels of accommodation. Myopia progression in the near corrected eyes was significantly slower than in the distance corrected eyes (intereye difference=0.36 D/year (95% CI: 0.54 to 0.19, p=0.0015, n=13); difference in VCD elongation=0.13 mm/year (95% CI: 0.18 to 0.08, p=0.0003, n=13)). After refitting with conventional spectacles, the resultant anisometropia returned to baseline levels after 9- 18 months. Conclusions: Monovision is not effective in reducing accommodation in juvenile myopia. However, myopia progression was significantly reduced in the near corrected eye, suggesting that sustained myopic defocus slows axial elongation of the human eye.

关键词：视力矫正光学离焦睫状肌麻痹等量球镜眼轴自动验光仪对侧眼矫正镜片玻璃体腔时用

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Active tracking of rejected dried blood samples in a large program in nigeria

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World Journal of Virology 2016年第2期5卷 73-81页

作者： Auchi Inalegwu Sunny phillips Rawlings Datir Christopher Chime Petronilla Ozumba Samuel Peters Obinna Ogbanufe Charles Mensah Alash'Le Abimiku Patrick Dakum nicaise ndembi Institute of Human Virology US Centers for Disease Control and Prevention Embassy of the United States of America Institute of Human Virology University of Maryland School of Medicine

AIM: To study the impact of rejection at different levels of health care by retrospectively reviewing records of dried blood spot samples received at the molecular laboratory for human immunodeficiency virus(HIV) early infant diagnosis(EID) between January 2008 and December ***: The specimen rejection rate, reasons for rejection and the impact of rejection at different levels of health care was examined. The extracted data were cleaned and checked for consistency and then deduplicated using the unique patient and clinic identifiers. The cleaned data were ciphered and exported to SPSS version 19(SPSS 2010 IBM Corp, new York, United States) for statistical ***: Sample rejection rate of 2.4%(n = 786/32552) and repeat rate of 8.8%(n = 69/786) were established. The mean age of infants presenting for first HIV molecular test among accepted valid samples was 17.83 wk(95%CI: 17.65-18.01) vs 20.30 wk(95%CI: 16.53-24.06) for repeated samples. HIV infection rate was 9.8% vs 15.9% for accepted and repeated samples. Compared to tertiary healthcare clinics, secondary and primary clinics had twofold and three-fold higher likelihood of sample rejection, respectively(P nificant increase in sample rejection rate with increasing number of EID clinics(r = 0.893, P = 0.041). The major reasons for rejection were improper sample collection(26.3%), improper labeling(16.4%) and insufficient blood(14.8%). COnCLUSIOn: Programs should monitor pre-analytical variables and incorporate continuous quality improvement interventions to reduce errors associated with sample rejection and improve patient retention.

关键词： Human immunodeficiency virus Prevention of mother-to-child transmission Early infant diagnosis Dried blood spot Pre-analytical error Sample rejection

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直接数字式合成器中寄生的抑制

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宇航计测技术 1983年第S1期 119-127页

作者： C.E.Wheatley D.E.phillips 谢蔚文

一、前言本文介绍一种能消除直接数字式合成器(DDS)输出中通常存在的离散寄生谱线的极好方法。所研究的 DDS 的型式主要由一个 n 位累加器组成,其容量在时钟频率 Fc 时增加一 K值。虽然在用这种方法来合成周期波方面有许多方案,但是,最... 详细信息

一、前言本文介绍一种能消除直接数字式合成器(DDS)输出中通常存在的离散寄生谱线的极好方法。所研究的 DDS 的型式主要由一个 n 位累加器组成,其容量在时钟频率 Fc 时增加一 K值。虽然在用这种方法来合成周期波方面有许多方案,但是,最简单可行的方案是只利用累加器溢出发生的时间来确定“方”波的两沿。对于幂次为2的输入数 K,输出频率是时钟频率的直接分谐波。但是,所有其它的数,电路的性能如同非均匀输出周期序列的分数分频器一样。这些周期序列和可确定的图形重复,以给出与所需信号周期相等的平均值。遗憾的是,寄生谱线或边带都是由输出的周期性调相所引起的。在过去,这样的谱线都是利用累加

关键词：累加器随机数谱线加法器乱数噪声谱时钟频率时钟脉冲寄生信号相位误差分谐波合成器

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