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Calculating the Crystallite Size of Microsorum scolopendria AgCl Nanoparticles and Their Biological Activities

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Journal of Biomaterials and Nanobiotechnology 2024年第2期15卷 25-37页

作者： Christian Nanga Chick Mohammed Mahdaly phillippe Belle Ebanda Kedi Kiuchi Sonoko Francois Eya’ane Meva Department of Materials and Life Sciences Faculty of Science and Technology Sophia University Tokyo Japan Laboratory of Innovative Nanostructured Material (NANO: C) Faculty of Medicine and Pharmaceutical Sciences The University of Douala Douala Cameroon Nanosciences African Network iThemba LABS-National Research Foundation Cape Town South Africa Department of Studies in Human Life Sciences Otsuma Womens University Tokyo Japan Institute for Inorganic Chemistry and Structural Chemistry Heinrich-Heine-University Dsseldorf Dsseldorf Germany

In this work, AgCl nanoparticles were synthesized from Microsorum scolopendria (MS) aqueous extract and AgNO3 solution. Preliminary confirmation was a color change from a light brown to a dark-colored solution and a UV-Vis spectra surface plasmon resonance peak at 427 nm. Measured vibrational frequencies at 1713 cm1 and 1030 cm1 for C-O stretching of carboxylic acid or aliphatic ketone, and 1547 cm1 for possibly N-O stretching of nitro compounds by Infrared (FTIR) analysis explain the possible biomaterial electronegative species or functional groups responsible for the reduction of Ag ( 1) to Ag (0) for the formation of MS-AgCl nanoparticles. XRD analysis studies revealed that these particles contained face-centered cubic crystallites of metallic AgCl of 100 % with an average calculated crystallite size range of 30.34 nm (SD = 5.10 nm) by Scherrers equation and a calculated crystallite size of 66.04 nm with a lattice strain of 0.00175 nm by Williamson Hall equation. The measured albumin denaturing activity of MS-AgCl nanoparticles gave an IC50 value of 26.70 g/mL and 1.35 g/mL for the positive control diclofenac. Additionally, the measured ability of phosphomolybdate complex formation, the antioxidant IC value of MS-AgCl nanoparticles was 35.29 g/mL, and positive control ascorbic acid was 13.91 g/mL. In all, using MS fern frond aqueous extracts, this preliminary work confirms MS-AgCl nanoparticles as potential therapeutic agents for oxidative stress, inflammatory problems, and related diseases.

关键词： Microsorum scolopendria SNPs IR UV-Vis Antioxidant

Prevalence and Factors Associated with Positivity of Antinuclear Antibodies (ANA) Patterns, Native Anti-DNA and Extractable Nuclear Antigens (ENA) Antibodies: Experience from a Laboratory in Dakar

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Open Journal of Rheumatology and Autoimmune Diseases 2024年第1期14卷 26-36页

作者： Diop Abdou Diallo Thierno Abdoulaye Ndiaye Babacar Mahou Chantal Diop Marième Gaye Dubrous phillippe Seck Abdoulaye Medical Biology Laboratory Pasteur Institute of Dakar Dakar Senegal Cheikh Anta Diop University of Dakar Dakar Senegal

Background: Diagnosis of autoimmune diseases (AID) is challenging, due to overlapping features with other non-immune disorders. Anti-nuclear antibodies (ANA) are sensitive screening tests but anti-deoxyribonucleic acid-antibody (anti-DNA), and anti-extractable nuclear antigens (anti-ENA) are specific for AIDs. We aimed to look at ANA patterns in our patients and correlated them with anti-ENA for proper interpretation and better patient management cost-effectively. Methods: A retrospective study was conducted over 1 year from January to December 2022 who were tested for ANA at biology medical laboratory of Pasteur Institute of Dakar. Anti-ENA and anti-DNA results were also analyzed for ANA-positive patients. Statistical analysis was performed using STATA 14.0, p Results: 216 patients were analyzed. Women predominated at 79.2% and mean age was 48 years [CI 95%, 46 - 50], with extremes of 10 and 89. Most represented age group was [41 - 60] with 38%. ANA was positive in 27 (12.5%) of patients, 59.2% of whom were strongly positive (titer of 1/1000, 1/3200 or 1/6400). The most common pattern was nuclear speckled, which was found in 77.8% of samples. Anti-ENA and anti-DNA positivity in ANA-positive patients was found respectively in 63% (17/27) and 1.4% (3/27) of the samples analyzed. Most commonly identified anti-ENA was anti-Sm 29.6%, anti-SSA 29.6%, anti-Ro-52 25.9%, anti-RNP 18.5% and anti-SSB 14.8% which was associated with speckled pattern. Association results indicated a significant relationship between both tests and between ANA titer in the anti-ENA- and ANA-positive patients (p 0.001). Conclusions: ANA, Anti-ENA and anti-DNA antibodies are essential for AIDS diagnosis. However, the testing repertoire should follow an algorithm comprising of clinical features, followed by ANA results with nuclear, mitotic, and cytoplasmic patterns, anti-ENA, and anti-DNA for a more meaningful, and cost-effective diagnostic approach.

关键词： Antinuclear Antibodies Extractable Nuclear antigen Autoimmune Disease Indirect Immunofluorescence

Bayesian inference of biomass growth characteristics for sugi(***)and hinoki(***)forests in self-thinned and managed stands

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Forest Ecosystems 2021年第4期8卷 1006-1023页

作者： Valerie Nicoulaud-Gouin Marc-Andre Gonze Pierre Hurtevent phillippe Calmon Institut de Radioprotection et de Surete Nucleaire St Paul-Lez-DuranceFrance

Background:Forests are an important sink for atmospheric carbon and could release that carbon upon deforestation and *** stand biomass dynamic of evergreen forests has become necessary to improve current biomass production *** different growth processes of managed forests compared to self-managed forests imply an adaptation of biomass prediction ***:In this paper we model through three models the biomass growth of two tree species(Japanese cedar,Japanese cypress)at stand level whether they are managed or not(self-thinning).One of them is named self-thinned model which uses a specific self-thinning parameterαand adapted to self-managed forests and an other model is named thinned model adapted to managed *** latter is compared to a Mitscherlich *** self-thinned model takes into account the light competition between trees relying on easily observable parameters(*** density).A Bayesian inference was carried out to determine parameters values according to a large database ***:In managed forest,Bayesian inference results showed obviously a lack of identifiability of Mitscherlich model parameters and a strong evidence for the thinned model in comparison to Mitscherlich *** self-thinning forest,the results of Bayesian inference are in accordance with the self-thinning 3/2 rule(α=1.4).Structural dependence between stand density and stand yield in self-thinned model allows to qualifying the expression of biological time as a function of physical time and better qualify growth and mortality *** mortality rate is 2.5 times more important than relative growth rate after about 40 years *** density and stand yield can be expressed as function of biological time,showing that yield is independent of initial ***:This paper addressed stand biomass dynamic models of evergreen forests in order to improve biomass growth dynamic assessment at regional scale relying on easily observable

关键词： Dynamic Biomass Self-thinning Sugi Hinoki Competition-Density rule

Effects of Cu^(2+)and humic acids on degradation and fate of TBBPA in pure culture of Pseudomonas *** CDT

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Journal of Environmental Sciences 2017年第12期29卷 60-67页

作者： Yini Ma Yingying Zhao Yongfeng Wang Xiangzhen Li Feifei Sun phillippe Francois-Xavier Corvini Rong Ji State Key Laboratory of Pollution Control and Resource Reuse School of the EnvironmentNanjing UniversityNanjing 210023China Key Laboratory of Environmental and Applied Microbiology Chengdu Institute of BiologyChinese Academy of SciencesChengdu 610041China Institute for Ecopreneurship School of Life SciencesUniversity of Applied Sciences and Arts Northwestern SwitzerlandGrundenstrasse 40Muttenz CH-4132Switzerland

Soil contamination with tetrabromobisphenol A（TBBPA） has caused great concerns;however, the presence of heavy metals and soil organic matter on the biodegradation of TBBPA is still unclear. We isolated Pseudomonas sp. strain CDT, a TBBPA-degrading bacterium, from activated sludge and incubated it with ^（14）C-labeled TBBPA for 87 days in the absence and presence of Cu^（2＋）and humic acids（HA）. TBBPA was degraded to organic-solvent extractable（59.4% ± 2.2%） and non-extractable（25.1% ± 1.3%） metabolites,mineralized to CO_2（4.8% ± 0.8%）, and assimilated into cells（10.6% ± 0.9%） at the end of incubation. When Cu^（2＋）was present, the transformation of extractable metabolites into non-extractable metabolites and mineralization were inhibited, possibly due to the toxicity of Cu^（2＋）to cells. HA significantly inhibited both dissipation and mineralization of TBBPA and altered the fate of TBBPA in the culture by formation of HA-bound residues that amounted to 22.1% ± 3.7% of the transformed TBBPA. The inhibition from HA was attributed to adsorption of TBBPA and formation of bound residues with HA via reaction of reactive metabolites with HA molecules, which decreased bioavailability of TBBPA and metabolites in the culture. When Cu^（2＋）and HA were both present, Cu^（2＋）significantly promoted the HA inhibition on TBBPA dissipation but not on metabolite degradation. The results provide insights into individual and interactive effects of Cu^（2＋）and soil organic matter on the biotransformation of TBBPA and indicate that soil organic matter plays an essential role in determining the fate of organic pollutants in soil and mitigating heavy metal toxicity.

关键词： Tetrabromobisphenol-A Biodegradation Soil organic matter Heavy metals Fate

Patterns of color phase indicate spawn timing at a Nassau grouper Epinephelus striatus spawning aggregation

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Current Zoology 2012年第1期58卷 73-83页

作者： Archer, Stephanie K Heppell, Scott A Semmens, Brice X Pattengill-Semmens, Christy V Bush, phillippe G Mccoy, Croy M Johnson, Bradley C Department of Fisheries and Wildlife 104 Nash Hall Oregon State University Corvallis OR 97331 USA Reef Environmental Education Foundation (REEF) PO Box 246 Key Largo Fl 33037 USA Department of Environment Cayman Islands Government P.O. Box 486GT Grand Cayman Cayman Islands

Nassau grouper Epinephelus striatus are a large bodied, top level predator that is ecologically important throughout the Caribbean. Although typically solitary, Nassau grouper form large annual spawning aggregations at predictable times in spe- cific locations. In 2003, The Cayman Islands Marine Conservation Board established protection for a newly rediscovered Nassau grouper spawning aggregation on Little Cayman, British West Indies. The large size of this aggregation provides a unique oppor- tunity to study the behavior of Nassau grouper on a relatively intact spawning aggregation. During non-spawning periods Nassau grouper display a reddish-brown-and-white barred coloration. However, while aggregating they exhibit three additional color phases：＂bicolor＂, ＂dark＂, and ＂white belly＂. We video sampled the population on multiple days leading up to spawning across five spawning years. Divers focused a laser caliper equipped video camera on individual fish at the aggregation. We later ana- lyzed the video to determine the length of the fish and record the color phase. Our observations show that the relative proportion of fish in the bicolor color phase increases significantly on the day leading up to the primary night of spawning. The increase in the proportion of the bicolor color phase from 0.05 early in the aggregation to 0.40 on the day of spawning suggests that this color phase conveys that a fish is behaviorally and physiologically prepared to spawn. Additionally, 82.7% of fish exhibiting dark or white belly coloration early in the aggregation period suggests that these color phases are not only shown by female fish as was previously posited [Current Zoology 58 （1）： 73-83, 2012].

关键词： Nassau grouper Epinephelus striatus Spawning aggregation Nuptial coloration Spawning behavior

Use,calibration and verification of agroecologicalmodels for boreal environments:A review

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Grassland Research 2022年第1期1卷 14-30页

作者： Daniel Forster Samuli Helama Matthew T.Harrison Clarence Alan Rotz Jinfeng Chang phillippe Ciais Elizabeth Pattey Perttu Virkajärvi Narasinha Shurpali Production Systems/Grasslands and Sustainable Farming Natural Resources Institute Finland(LUKE)MaaninkaFinland Natural Resources Institute Finland(Luke) RovaniemiFinland Tasmanian Institute of Agriculture University of TasmaniaLauncestonTasmaniaAustralia USDA/Agricultural Research Service University ParkPennsylvaniaUSA College of Environmental and Resource Sciences Zhejiang UniversityHangzhouChina Laboratoire des Sciences du Climat et de l'Environnment LSCE/IPSLCEA-CNRS-UVSQUniversitéParis-SaclayGif-sur-YvetteFrance Agriculture and Agri-Food Canada Ottawa Research and Development CentreCentral Experimental FarmOttawaOntarioCanada

Past assessments report negative impacts of the climate crisis in boreal areas;but milder and shorter winters and elevated atmospheric CO_(2) may provide opportunities for agricultural productivity potentially playing a significant role in future food *** cropping systems are expanding in boreal areas,but the regional mainstay will likely continue to be livestock *** models can when appropriately calibrated and evaluated,facilitate improved productivity while minimising environmental impacts by identifying system interactions,and quantifying greenhouse gas emissions,soil carbon stocks and fertiliser *** models designed for temperate and tropical zones abound,few are developed specifically for boreal zones,and there is uncertainty around the performance of existing models in boreal *** reviewed model performance across boreal environments and management *** identified a dearth of modelling studies in boreal regions,with the publication of three or less papers per year since the year 2000,constituting a significant research *** IFSM and BASGRA_N performed best in grassland production,DNDC best in predicting soil N_(2)O and NH_(3) *** model outperformed all others,strengthening the case for ensemble *** agroecological models would be worthy of further evaluation,providing model improvements designed for boreal systems.

关键词： boreal region carbon-nitrogen cycling ecophysiological modelling greenhouse gas emissions soil carbon

Spatiotemporal dynamics of ecosystem fires and biomass burning-induced carbon emissions in China over the past two decades

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Geography and Sustainability 2020年第1期1卷 47-58页

作者： Anping Chen Rongyun Tang Jiafu Mao Chao Yue Xiran Li Mengdi Gao Xiaoying Shi Mingzhou Jin Daniel Ricciuto Sam Rabin phillippe Ciais Shilong Piao Department of Biology Colorado State UniversityFort CollinsCO 80523USA Graduate Degree Program in Ecology Colorado State UniversityFort CollinsCO 80523USA Department of Industrial and Systems Engineering University of TennesseeKnoxvilleTN 37996USA Environmental Sciences Division and Climate Change Science Institute Oak Ridge National LaboratoryOak RidgeTN 37831USA State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau Northwest Agriculture&Forestry UniversityYanglingShanxi 712100China College of Urban and Environmental Sciences Central China Normal UniversityWuhanHubei 430079China Sino-French Institute for Earth System Science College of Urban and Environmental SciencesPeking UniversityBeijing 100871China Atmospheric Environmental Research(IMK-IFU) Institute of Meteorology and Climate ResearchKarlsruhe Institute of Technology(KIT)Kreuzeckbahnstraße 19Garmisch-Partenkirchen 82467Germany LSCE UMR CEA-CNRSBat.709CEL’Orme des MerisiersGif-sur-Yvette 91191France

Fire is a major type of disturbance that has important influences on ecosystem dynamics and carbon *** our understanding of ecosystem fires and their carbon cycle consequences is still limited,largely due to the difficulty of large-scale fire monitoring and the complex interactions between fire,vegetation,climate,and anthropogenic ***,using data from satellite-derived fire observations and ecosystem model simulations,we performed a comprehensive investigation of the spatial and temporal dynamics of China’s ecosystem fire disturbances and their carbon emissions over the past two decades(1997–2016).Satellite-derived results showed that on average about 3.47-4.53×10^(4) km^(2) of the land was burned annually during the past two decades,among which annual burned forest area was about 0.81-1.25×10^(4) km^(2),accounting for 0.33-0.51%of the forest area in *** burning emitted about 23.02 TgC per *** to satellite products,simulations from the Energy Exascale Earth System Land Model(ELM)strongly overestimated China’s burned area and fire-induced carbon *** burned area and fire-induced carbon emissions were high for boreal forest in Northeast China’s Daxing’anling region and subtropical dry forest in South Yunnan,as revealed by both the satellite product and the model *** results suggest that climate and anthropogenic factors play critical roles in controlling the spatial and seasonal distribution of China’s ecosystem fire *** findings highlight the importance of multiple complementary approaches in assessing ecosystem fire disturbance and its carbon *** studies are required to improve the methods of observing and modelling China’s ecosystem fire disturbances,which will provide valuable information for fire management and ecosystem sustainability in an era when both human activities and the natural environment are rapidly changing.

关键词： Fire emission Burned area Fire models China

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ADSL调制解调器的保护线路接口设计

电子设计应用 2003年第9期 66-67页

作者： phillippe Rabier Stephane Serrier ED Online ED Online

异步数字用户线路(ADSL)调制解调器由于同普通电话线相连,容易遭受电击.因此,必须施加恰当保护,以抵抗破坏性过压和压流的冲击.通晓保护标准、指导原则和器件,可以提高设计人员保护ADSL调制解调器的电话线及数据线免遭过压冲击的能力.

关键词： ADSL 调制解调器保护线路接口设计异步数字用户线路

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KLOOF 145 开普敦斜坡上的风景房

现代装饰 2019年第8期 122-127页

作者： phillippe Fouché Adam Letch(摄影) 苏烨(编译) 不详

Kloof 145风景房坐落于Lion’s Head大街向西方向的斜坡上,地处开普敦郊外。地理位置的优越性让它更好地抵御西风的影响。斜坡原本被森林所覆盖,现已开发成另一番壮阔的景色:沙滩、海边裸露的礁石、朝向南面的Twelve Apostles山脉皆在'... 详细信息

Kloof 145风景房坐落于Lion’s Head大街向西方向的斜坡上,地处开普敦郊外。地理位置的优越性让它更好地抵御西风的影响。斜坡原本被森林所覆盖,现已开发成另一番壮阔的景色:沙滩、海边裸露的礁石、朝向南面的Twelve Apostles山脉皆在'沐浴'夕阳。'这个项目的设计理念旨在还原自然本质。'SAOTA建筑设计所南非主案设计师phillippe Fouché如是说。住宅底层是一个独立的结构,它为住宅提供坚固的岩石地基,形成独特的特色。

关键词：开普敦 KLOOF 145