检索结果-南通市图书馆

Complexity of molecular alterations impacts pancreatic cancer prognosis

World Journal of Gastrointestinal Oncology 2013年第1期5卷 1-3页

作者： Ivonne Regel Bo Kong philipp bruns Christoph W Michalski Jrg Kleeff Department of Surgery Technische Universitt München

Individualized cancer treatment(*** therapy) based on molecular alterations has emerged as an important strategy to improve the current standardof-care chemotherapy.A large number of studies have demonstrated the importance of biomarkers not only in predicting prognosis but more importantly in predicting the response towards *** example,amplification or mutation status of the two biomarkers HER2(human epidermal growth factor 2) and BRCA(breast cancer) can be used to decide on a specific targeted therapy in breast ***,no biomarkers with a similar clinical impact have been identified in pancreatic ductal *** many genome-wide and proteome-based high-throughput studies have identified candidate genes or proteins as promising biomarkers,none of them were eventually transferred into the clinical ***,the most reliable markers for predicting prognosis are still the tumor stage and grade and biomarkers for therapy response remain *** reason lies in the lack of systemic approaches to analyze the complexity of dominating cancer pathways and the impact of such signal complexity on prognosis and therapy response.

关键词： Pancreatic cancer Diagnostic markers Biomarkers Targeted therapy Prognosis Pathways

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Dual-sugar tests of small intestinal permeability are poor predictors of bacterial infections and mortality in cirrhosis: a prospective study

引用

World Journal of Gastroenterology 2016年第11期22卷 3275-3284页

作者： Anika Vogt philipp A Reuken Sven Stengel Andreas Stallmach Tony bruns Department of Internal Medicine IV (Gastroenterology Hepatologyand Infectious Diseases)Jena University Hospital Center for Sepsis Control and Care Jena University Hospital

AIM: To prospectively analyze the impact of increased intestinal permeability (IP) on mortality and the occurrence of infections in patients with ***: IP was quantified using the lactulose/mannitol (L/M) test in 46 hospitalized patients with cirrhosis (25 Child-Pugh A/B, 21 Child-Pugh C) and in 16 healthy controls. Markers of inflammation [LPS-binding protein, Interleukin-6 (IL-6)] and enterocyte death [intestinal fatty-acid binding protein (I-FABP)] were determined in serum using enzyme-linked immunosorbent assays. Patients were followed for one year and assessed for survival, liver transplantation, the necessity of hospitalization and the occurrence of bacterial infections. The primary endpoint of the study was defined as differences in survival between patients with pathological and without pathological lactulose/mannitol ***: Thirty-nine (85%) patients with cirrhosis had a pathologically increased IP index (L/M ratio > 0.07) compared to 4 (25%) healthy controls (P < 0.0001). The IP index correlated with the Child-Pugh score (r = 0.484, P = 0.001) and with serum IL-6 (r = 0.342, P = 0.02). Within one year, nineteen (41%) patients developed a total of 33 episodes of hospitalization with bacterial or fungal infections. Although patients who developed spontaneous bacterial peritonitis (SBP) (n = 7) had a higher IP index than patients who did not (0.27 vs 0.14, P = 0.018), the baseline IP index did not predict time to infection, infection-free survival or overall survival, neither when assessed as linear variable, as tertiles, nor dichotomized using an established cut-off. In contrast, model for end-stage liver disease score, Child-Pugh score, the presence of ascites, serum IL-6 and I-FABP were univariate predictors of infection-free ***: Although increased IP is a frequent phenomenon in advanced cirrhosis and may predispose to SBP, it failed to predict infection-free and overall survival in this prospective coho

关键词： Intestinal permeability Liver cirrhosis Lactulose/mannitol ratio Spontaneous bacterial peritonitis Bacterial translocation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Liver-specific gene expression in mesenchymal stem cells is induced by liver cells

引用

World Journal of Gastroenterology 2005年第29期11卷 4497-4504页

作者： Claudia Lange philipp Bassler Michael V. Lioznov Helge bruns Dietrich Kluth Axel R. Zander Henning C. Fiegel Center of Bone Marrow Transplantation University Hospital Hamburg-Eppendorf Martinistrasse 52 D-20246 Hamburg Germany Department of Pediatric Surgery University Hospital Hamburg-Eppendorf Martinistrasse 52 D-20246 Hamburg Germany

AIM： The origin of putative liver cells from distinct bone marrow stem cells, e.g. hematopoietic stem cells or multipotent adult progenitor cells was found in recent in vitro studies. Cell culture experiments revealed a key role of growth factors for the induction of liver-specific genes in stern cell cultures. We investigated the potential of rat mesenchymal stem cells （MSC） from bone marrow to differentiate into hepatocytic cells in vitro. Furthermore, we assessed the influence of cocultured liver cells on induction of liver-specific gene expression. METHODS： Mesenchymal stem cells were marked with green fluorescent protein （GFP） by retroviral gene transduction. Clonal marked MSC were either cultured under liver stimulating conditions using fibronectin-coated culture dishes and medium supplemented with SCF, HGF, EGF, and FGF-4 alone, or in presence of freshly isolated rat liver cells. Cells in cocultures were harvested and GFP＋ or GFP- cells were separated using fluorescence activated cell sorting. RT-PCR analysis for the stem cell marker Thyl and the hepatocytic markers CK-18, albumin, CK-19, and AFP was performed in the different cell populations. RESULTS： Under the specified culture conditions, rat MSC cocultured with liver cells expressed albumin-, CK-18, CK-19, and AFP-RNA over 3 weeks, whereas MSC cultured alone did not show liver specific gene expression, CONCLUSION： The results indicate that （1） rat MSC from bone marrow can differentiate towards hepatocytic lineage in vitro, and （2） that the microenvironment plays a decisive role for the induction of hepatic differentiation of rMSC.

关键词： Mesenchymal stem cells Liver-specific differentiation Coculture

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

手小关节病高分辨MRI特征——骨性关节炎和银屑病性关节炎的鉴别限度

引用

国际医学放射学杂志 2013年第4期 406-406页

作者： L.S. Braum D. McGonagle A. bruns S. philipp S. Hermann K. Aupperle 范璐 Department of Radiology Charité Medical School Campus MitteCharitéplatz 110117 Berlin Germany

目的检验如下假设:在临床实践中,可以应用高分辨MRI依据关节病变定位的组织学差异鉴别类风湿性关节炎(RA)、脊椎关节炎/银屑病性关节炎(SpA/PsA)和骨性关节炎(OA)。方法对69例疑诊手或足炎性关节病的病人行高分辨MRI检查,使用小环形线... 详细信息

目的检验如下假设:在临床实践中,可以应用高分辨MRI依据关节病变定位的组织学差异鉴别类风湿性关节炎(RA)、脊椎关节炎/银屑病性关节炎(SpA/PsA)和骨性关节炎(OA)。方法对69例疑诊手或足炎性关节病的病人行高分辨MRI检查,使用小环形线圈。在不知道临床资料的前提下对影像进行盲法评分。记录不同的关节改变,如骨膜炎、骨炎、骨侵蚀、起止点炎和滑膜炎。将基于影像的诊断和临床诊断进行比较。结果 59.4%临床诊断病人经影像分析确认。OA高达80%,RA中等为67%,SpA/PsA仅为50%。主要的难点在于鉴别OA和SpA/PsA,因为它们有共同的影像表现,包括骨膜炎(SpA/PsA为45%,OA为40%,而RA为0;P=0.015)。同样,骨炎也常见于SpA/PsA(79%)和OA(80%),而少见于RA(42%)(P=0.014)。结论仅使用高分辨MRI鉴别小关节炎性病变的特征仍存挑战,尤其当鉴别OA和PsA时。这些表现或许应归因于病变表达的组织学相似而非影像技术存在限度。要点①高分辨MRI越来越多地应用于关节病变的研究。②骨炎和骨膜炎发生于银屑病性关节炎和骨性关节炎(但不会发生于类风湿关节炎)。③病变严重的病人中骨膜炎和骨侵蚀的数量相近。

关键词：高分辨MRI 类风湿关节炎脊椎关节炎银屑病性关节炎骨性关节炎

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：