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Development of Highly Efficient Transformation System of Yeast-Like Conidia of Tremella fuciformis

Agricultural Sciences in China 2009年第3期8卷 268-275页

作者： GUO Li-qiong LIU er-xian WANG Jie LIN Jun-fang Department of Bioengineering College of Food Science South China Agricultural University Guangzhou 510640 P.R. China Institute of Biomass Research South China Agricultural University Guangzhou 510640 P.R.China

Tremellafuciformis is one of higher basidiomycetes. Its basidiospore can reproduce yeast-like conidia, which is also called the blastospore by budding. The yeast-like conidia of T. fuciformis is monokaryotic and easy to culture by submerged fermentation similar to yeast. Thus, it is a good recipient cell for exogenous gene expression. In this study, the expression plasmid pAN7-1 （containing promoter gpd-An derived from Aspergillus nidulans and selectable marker gene hph conferring resistance to hygromycin B） and plasmid pLg-hph （containing promoter gpd-Le derived from Lentinula edodes and selectable marker gene hph） were transformed into the yeast-like conidia of T. fuciformis by pEG-mediated protoplast transformation, respectively. The primary putative transformants were selected by the sandwich screening method with the selective medium containing 50 μg mL^-1 hygromycin. The putative transformants were obtained from the primary putative transformants transferred on pDSA plates containing 100 μg mL^-1 hygromycin for second round selection. Experimental results showed that the optimal concentration of pEG 4000 for mediating protoplast transformation was 25%. pCR and Southern blotting confirmed that the selectable marker gene hph was integrated effectively into the genome of the yeast-like conidia of T. fuciformis with plasmid pLg-hph transformation. Its transformation efficiency was 110 transformants per μg DNA, and the hph gene was integrated into the genome of some yeast-like conidia with plasmid pAN7-1 transformation. However, its transformation efficiency was only 9 transformants per μg DNA. The presence of hph gene in the genome of transformants after 5 generations of sub- culturing on pDSB medium was confirmed by pCR, suggesting that the foreign gene hph was stable during subculture.

关键词： Tremellafuciformis pEG-mediated protoplast transformation yeast-like conidia hph gene promoter

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Sodium 4-phenylbutyrate Attenuates High-fat Diet-induced Impaired Spermatogenesis

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Biomedical and Environmental Sciences 2018年第12期31卷 876-882页

作者： WANG er Hui YAO San Qiao TAO Ling XI Jin Yan Department of Nutrition and Food Hygiene College of Public Health Xinxiang Medical University Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine Xinxiang Medical University

Objective To determine the mitigating effects of sodium 4-phenylbutyrate(4-pBA) on high-fat diet(HFD)-induced spermatogenesis dysfunction. Methods Male rats(n = 30) were randomly divided into three groups: control, HFD, and 4-pBA(HFD +4-pBA). After 13 weeks, rats were euthanized. Testes and epididymis were harvested for further analysis. Sex hormones were detected, and hematoxylin and eosin staining was performed to examine the histological changes in the testes. Semen samples were collected to evaluate sperm quality. Spermatogenic cell apoptosis was detected by TUNEL assay. Results Compared with the control group, the final body weight and body weight gain were significantly higher in HFD-fed rats, while the testicle/body weight ratios were lower(p pathological changes in the testicular tissue were observed. Treatment with 4-pBA attenuated HFD-induced histological damage, ameliorated the HFD-induced decrease in serum testosterone(T), and reduced the rate of testicular cell apoptosis(p pBA significantly improved semen parameters in HFD rats(p posure induced detrimental effects on spermatogenesis, semen quality, serum T level, and testicular cell apoptosis in rats. Treatment with 4-pBA ameliorated HFD-induced impaired spermatogenesis via inhibition of apoptosis in rats. 4-pBA may have therapeutic value in the treatment of obesity-related impairment of spermatogenesis.

关键词： Male infertility Sodium 4-phenylbutyrate Obesity Spermatogenesis Semen Testis

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Synthesis of polysubstituted 1,4-Dihydropyridines via Four-component Reactions of Arylamine,Acetylenedicarboxylate, Aromatic Aldehyde and Ethyl Acetoacetate

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Chemical Research in Chinese Universities 2012年第4期28卷 652-655页

作者： WU ping XIA er-yan SUN Jing YAN Chao-guo Department of Biochemical Engineering Yangzhou Polytechnic UniversityYangzhou 225000P.R.China College of Chemistry&Chemical Engineering Yangzhou UniversityYangzhou 225002P..R.China

An efficient synthetic protocol for the 1,4-diaryl-1,4-dihydropyridines was developed via the domino four-component reaction of arylamine,acetylenedicarboxylate,aromatic aldehyde and ethyl *** reaction mechanism invol... 详细信息

关键词： 1 4-Dihydropyridine β-Enamino ester Domino reaction Four-component reaction

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An efficient data layout scheme for better I/O balancing in RAID-6 storage systems

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Frontiers of Information Technology & Electronic Engineering 2015年第5期16卷 335-345页

作者： ping XIE Jian-zhong HUANG er-wei DAI Qiang CAO Chang-sheng XIE Wuhan National Laboratory for Opto Electronics Huazhong University of Science & Technology Computer College Qinghai Normal University

Among redundant arrays of independent disks(RAID)-6 codes, maximum distance separable(MDS)based RAID-6 codes are popular because they have the optimal storage efficiency. Although vertical MDS codes exhibit better load balancing compared to horizontal MDS codes in partial stripes, an I/O unbalancing problem still exists in some vertical codes. To address this issue, we propose a novel efficient data layout, uniform p-code(UpC), to support highly balanced I/Os among p-coded disk arrays(i.e., pC). In UpC, the nonuniformly distributed information symbols in each parity chain of p-code are moved along their columns to other rows, thus enabling the parity chain to keep original parity relationships and tolerate double disk failures. The UpC scheme not only achieves optimal storage efficiency, computational complexity, and update complexity, but also supports better I/O balancing in the context of large-scale storage systems. We also conduct a performance study on reconstruction algorithms using an analytical model. Besides extensive theoretical analysis, comparative performance experiments are conducted by replaying real-world workloads under various configurations. Experimental results illustrate that our UpC scheme significantly outperforms the pC scheme in terms of average user response time. In particular, in the case of a 12-disk array, the UpC scheme can improve the access performance of the RAID-6 storage system by29.9% compared to the pC scheme.

关键词： RAID-6 Data availability High performance I/O balancing

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Impacts of formal credit on rural household income:Evidence from deprived areas in western China

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Journal of Integrative Agriculture 2021年第4期20卷 927-942页

作者： CHEN Si LUO er-ga Lita ALITA HAN Xiao NIE Feng-ying College of Mathematics and Informatics South China Agricultural UniversityGuangzhou 510642P.R.China Department of Agricultural Economics and Management School of Public AffairsChina Academy for Rural Development(CARD)Zhejiang UniversityHangzhou 310000P.R.China Environmental policy Group Wageningen UniversityHollandseweg 16706KN Wageningen China Agricultural Science and Technology press Chinese Academy of Agricultural SciencesBeijing 100081P.R.China Agricultural Information Institute Chinese Academy of Agricultural SciencesBeijing 100081P.R.China

Financial support is a crucial part of China's poverty alleviation ***,it is vital to understand how formal credit impacts income growth in rural *** 2012,2015,and 2018,a survey was conducted to obtain a panel dataset of 592 rural households from 6 poverty-stricken counties in western China,including counties in Guizhou,Yunnan,and Shaanxi *** use the data to examine the effect of formal credit on rural household income and the mechanism that underlies this *** find that formal credit can significantly increase rural households'income in deprived areas in western ***,formal credit promotes the reallocation of household labor from the agricultural sector to the non-agricultural sector and changes rural households'decisions about investment-consumption *** are the drivers of changes in the amount and structure of household *** analyses show that formal credit may widen income inequality among rural households in western China's deprived *** individual characteristics of rural households,such as different levels of material capital,human capital,and social capital,bring about differences in the effects of formal credit on income *** study emphasizes that the implementation of formal credit is an essential strategy for poverty alleviation in underdeveloped areas,but policymakers should not excessively interfere with the financial market.

关键词： formal credit rural household income rural finance poverty-stricken areas in western China

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脑卒中患者采取数字OT训练系统与作业疗法干预对上肢功能和生活自理能力的影响分析

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黑龙江医学 2024年第3期48卷 336-339页

作者：王梦露尔兆娟肖中兴天津市宝坻区人民医院康复医学科天津301800

目的:探讨数字OT训练系统与作业疗法在脑卒中疾病中联合应用,对生活自理能力(ADL)评分和上肢功能的影响。方法:选取2020年5月—2021年5月天津市宝坻区人民医院收治的68例脑卒中患者作为研究对象,按照随机数表法分为对照组和观察组,每组... 详细信息

目的:探讨数字OT训练系统与作业疗法在脑卒中疾病中联合应用,对生活自理能力(ADL)评分和上肢功能的影响。方法:选取2020年5月—2021年5月天津市宝坻区人民医院收治的68例脑卒中患者作为研究对象,按照随机数表法分为对照组和观察组,每组各34例。对照组采取作业疗法,观察组采取作业疗法联合数字OT训练系统疗法。对比两组患者康复效果、训练配合度、ADL评分、肢体运动功能(Fugl-Meyer)评分、神经功能(NIHSS)评分、认知功能(MoCA)评分、健康生活质量(SF-36)评分等指标。结果:观察组患者临床康复效果高于对照组,差异有统计学意义(χ^(2)=5.100,p>0.05)。观察组患者训练配合度明显高于对照组,差异有统计学意义(χ^(2)=4.220,p>0.05)。干预后,观察组患者ADL评分、上肢功能评分高于对照组,差异有统计学意义(t=7.035、3.475,ppp<0.05)。结论:脑卒中患者采取数字OT训练系统与作业疗法联合干预的效果明显,可改善其认知、神经功能,经合理训练后其上肢功能恢复明显,提升患者日常ADL、生活质量及训练配合度。

关键词：脑卒中数字OT训练系统作业疗法上肢功能生活自理能力

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人性化护理服务模式应用于眼科门诊护理工作中的价值分析

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中外医疗 2024年第18期43卷 124-127页

作者：张美丽尔梦娜王倩怡天津医科大学附属宝坻医院门诊部天津301800 天津医科大学附属宝坻医院脊柱关节外科天津301800

目的考察人性化护理服务模式实施在眼科门诊护理工作中的效果。方法回顾性选取天津医科大学附属宝坻医院于2021年1月—2023年1月收治的72例患者的临床资料,根据护理方式的不同,分为对照组(n=36,常规护理)和观察组(n=36,人性化护理),比... 详细信息

目的考察人性化护理服务模式实施在眼科门诊护理工作中的效果。方法回顾性选取天津医科大学附属宝坻医院于2021年1月—2023年1月收治的72例患者的临床资料,根据护理方式的不同,分为对照组(n=36,常规护理)和观察组(n=36,人性化护理),比较两组患者的遵医行为、不良情绪评分、疼痛评分、生活质量评分以及护理满意度。结果观察组的总依从率为97.22%,高于对照组的77.78%,差异有统计学意义(χ^(2)=4.571,pp均p<0.05)。结论在眼科门诊护理工作中实施人性化护理服务模式,可显著提升患者的依从性以及护理满意度,并降低其不良情绪与疼痛感。

关键词：人性化护理不良情绪疼痛评分

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The Chinese Society of Clinical Oncology (CSCO): Clinical guidelines for the diagnosis and treatment of gastric cancer, 2023

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Cancer Communications 2024年第1期44卷 127-172页

作者： Feng-Hua Wang Xiao-Tian Zhang Lei Tang Qi Wu Mu-Yan Cai Yuan-Fang Li Xiu-Juan Qu Hong Qiu Yu-Jing Zhang Jie-er Ying Jun Zhang Ling-Yu Sun Rong-Bo Lin Chang Wang Hao Liu Miao-Zhen Qiu Wen-Long Guan Sheng-Xiang Rao Jia-Fu Ji Yan Xin Wei-Qi Sheng Hui-Mian Xu Zhi-Wei Zhou Ai-ping Zhou Jing Jin Xiang-Lin Yuan Feng Bi Tian-Shu Liu Han Liang Yan-Qiao Zhang Guo-Xin Li Jun Liang Bao-Rui Liu Lin Shen Jin Li Rui-Hua Xu Department of Medical Oncology Sun Yat-sen University Cancer CenterState Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangzhouGuangdongP.R.China Department of Gastrointestinal Oncology Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education)Peking University Cancer HospitalBeijingP.R.China Department of Radiology Peking University Cancer HospitalBeijingP.R.China Department of Endoscopy Center Peking University Cancer HospitalBeijingP.R.China Department of pathology Sun Yat-sen University Cancer CenterState Key Laboratory of Oncology in South ChinaCollaborative Innovation Center of Cancer MedicineGuangzhouGuangdongP.R.China Department of Gastric Surgery Sun Yat-sen University Cancer CenterState Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangzhouGuangdongP.R.China Department of Medical Oncology The First Hospital of China Medical UniversityShenyangLiaoningP.R.China Department of Medical Oncology Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and TechnologyWuhanHubeiP.R.China Department of Radiotherapy Sun Yat-sen University Cancer CenterState Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangzhouGuangdongP.R.China Department of Medical Oncology Zhejiang Cancer HospitalHangzhouZhejiangP.R.China Department of Medical Oncology Ruijin HospitalShanghai Jiaotong University School of MedicineShanghaiP.R.China Department of Surgical Oncology The Fourth Affiliated Hospital of Harbin Medical UniversityHarbinHeilongjiangP.R.China Department of Medical Oncology Fujian Cancer HospitalFuzhouFujianP.R.China Tumor Center The First Hospital of Jilin UniversityChangchunJilinP.R.China Department of General Surgery Nanfang HospitalSouthern Medical UniversityGuangzhouGuangdongP.R.China Department of Radiology Zhongshan HospitalFudan UniversityShanghaiP.R.China Department of

The 2023 update of the Chinese Society of Clinical Oncology(CSCO)Clini-cal Guidelines for Gastric Cancer focuses on standardizing cancer diagnosis and treatment in China,reflecting the latest advancements in evidence-based medicine,healthcare resource availability,and precision *** updates address the differences in epidemiological characteristics,clinicopatho-logical features,tumor biology,treatment patterns,and drug selections between Eastern and Western gastric cancer *** revisions include a structured template for imaging diagnosis reports,updated standards for molecular marker testing in pathological diagnosis,and an elevated recommendation for neoadju-vant chemotherapy in stage III gastric *** advanced metastatic gastric cancer,the guidelines introduce new recommendations for immunotherapy,anti-angiogenic therapy and targeted drugs,along with updated management strategies for human epidermal growth factor receptor 2(Her2)-positive and deficient DNA mismatch repair(dMMR)/microsatellite instability-high(MSI-H)***,the guidelines offer detailed screening recommendations for hereditary gastric cancer and an appendix listing drug treatment regimens for various stages of gastric *** 2023 CSCO Clinical Guidelines for Gastric Cancer updates are based on both Chinese and international clinical research and expert consensus to enhance their applicability and relevance in clinical practice,particularly in the heterogeneous healthcare landscape of China,while maintaining a commitment to scientific rigor,impartiality,and timely revisions.

关键词： Chinese Society of Clinical Oncology(CSCO) gastric cancer diagnosis surgery neoadjuvant adjuvant radiotherapy chemotherapy targeted therapy immunotherapy

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新辅助化疗联合免疫治疗对食管癌根治术患者围术期肾功能的影响

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中华麻醉学杂志 2024年第8期44卷 932-936页

作者：王凯元涂慧芳邓城旗关山耳建旭尹毅青天津医科大学肿瘤医院麻醉科国家恶性肿瘤临床医学研究中心天津市恶性肿瘤临床医学研究中心天津市肿瘤防治重点实验室天津300060 天津中医药大学研究生院天津301617 天津大学胸科医院麻醉科天津300222

目的评价新辅助化疗联合免疫治疗对食管癌根治术患者围术期肾功能的影响。方法本研究为回顾性队列研究。收集2020年1月至2022年4月于天津医科大学肿瘤医院和天津大学胸科医院行食管癌根治术患者的临床资料,根据患者术前治疗方案分为2组... 详细信息

目的评价新辅助化疗联合免疫治疗对食管癌根治术患者围术期肾功能的影响。方法本研究为回顾性队列研究。收集2020年1月至2022年4月于天津医科大学肿瘤医院和天津大学胸科医院行食管癌根治术患者的临床资料,根据患者术前治疗方案分为2组:新辅助化疗组(nCT组)和新辅助化学联合免疫治疗组(nCT+IT组)。nCT组接受以铂类药为基础,结合氟尿嘧啶或紫杉醇的新辅助化疗;nCT+IT组在接受新辅助化疗的基础上,联合使用程序性死亡受体1抗体药物进行免疫治疗。患者均接受了2~3个周期的治疗,每个周期21 d,在治疗完成后4~6周进行手术。收集治疗前、术前72 h及术后72 h的血清肌酐、尿酸及尿素浓度。采用KDIGO标准诊断急性肾损伤(AKI),记录术前72 h及术后72 h时AKI发生情况。收集患者术后病理完全缓解率、复发率和无病生存时间。结果与nCT组比较,nCT+IT组治疗后血清尿素浓度升高,术前72 h和术后72 h时血清尿酸浓度升高,术后病理完全缓解率升高,复发率降低,无病生存时间延长(pp>0.05)。结论新辅助化疗联合免疫治疗虽然可提高食管癌根治术患者病理完全缓解率和无病生存率,但对肾功能会产生一定的影响,围术期应加强肾功能检测,防止AKI的发生。

关键词：新辅助化疗免疫疗法食管肿瘤肾功能试验

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生肌止痛方坐浴联合紫草纱条创面引流对小儿肛周脓肿术后患者的临床疗效

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中成药 2024年第1期46卷 121-125页

作者：位艳赏刘红振袁义朋白二龙王燕燕河北省中医院肛肠外科河北石家庄050000 华北医疗健康集团石家庄华药医院肛肠科河北石家庄050000 河北省深州市医院普外科河北石家庄053800 北京京煤集团总医院普外科北京102300

目的探讨生肌止痛方坐浴联合紫草纱条创面引流对小儿肛周脓肿术后患者的临床疗效。方法100例患者随机分为对照组和观察组,每组50例,对照组给予常规治疗,观察组给予生肌止痛方坐浴联合紫草纱条创面引流。检测创面水肿评分、创面分泌物评... 详细信息

目的探讨生肌止痛方坐浴联合紫草纱条创面引流对小儿肛周脓肿术后患者的临床疗效。方法100例患者随机分为对照组和观察组,每组50例,对照组给予常规治疗,观察组给予生肌止痛方坐浴联合紫草纱条创面引流。检测创面水肿评分、创面分泌物评分、创面肉芽组织生长评分、FLACC评分、中医证候评分、创面愈合情况(渗出物消失时间、创面水肿消失时间、创面愈合时间)、pCT、TNF-α、sTREM-1、白细胞、Sp、NpY、5-HT、肛门功能指标(直肠最大耐受容量、直肠肛管抑制反射阈、肛管高压带长度)变化。结果观察组创面愈合情况优于对照组(ppCT、TNF-α、sTREM-1、白细胞、Sp、NpY、5-HT降低(ppppp<0.05)。结论生肌止痛方坐浴联合紫草纱条创面引流可抑制小儿肛周脓肿术后患者机体炎症,促进创面愈合,提升肛门功能。

关键词：生肌止痛方紫草纱条坐浴创面引流小儿肛周脓肿

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