检索结果-南通市图书馆

Fatty acid hydratase for value-added biotransformation:A review

Chinese Journal of Chemical Engineering 2020年第8期28卷 2051-2063页

作者： Yan Zhang Bekir Engin Eser peter kristensen Zheng Guo Department of Engineering Faculty of Science and TechnologyAarhus UniversityGustav wieds vej 108000 AarhusDenmark Faculty of Engineering and Science Department of Chemistry and BioscienceAalborg University9220 AalborgDenmark

The synthesis of hydroxy fatty acids(HFAs) from renewable oil feedstock by addition of water onto C_C bonds has attracted great attention in recent years. Given that selective asymmetric hydration of non-activated C_C bonds has been proven difficult to achieve with chemical catalysts, enzymatic catalysis by fatty acid hydratases(FAHs) presents an attractive alternative approach to produce value-added HFAs with high regio-, enantioand stereospecificity, as well as excellent atom economy. Even though FAHs have just been investigated as a potential biocatalyst for a decade, remarkable information about FAHs in different aspects is available;however, a comprehensive review has not been archived. Herein, we summarize the research progresses on biochemical characterization, structural and mechanistic determination, enzyme engineering, as well as biotechnological application of FAHs. The current challenges and opportunities for an efficient utilization of FAHs in organic synthesis and industrial applications are critically discussed.

关键词： Hydroxy fatty acids Fatty acid hydratases Enzyme characterization Catalytic mechanism Enzyme engineering Biotransformation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Net absorption and liver metabolism of amino acids and heat production of portaldrained viscera and liver in multiparous sows during transition and lactation

引用

Journal of Animal Science and Biotechnology 2020年第2期11卷 424-439页

作者： Liang Hu Niels Bastian kristensen Lianqiang Che De Wu peter Kappel Theil Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education Institute of Animal NutritionSichuan Agricultural UniversityNo.211Huimin RoadWenjiang DistrictChengduSichuan 611130People’s Republic of China Department of Animal Science Faculty of Science and TechnologyAarhus UniversityDK-8830 TjeleDenmark Present address:SEGES Danish Pig Research Centre DK-1609 CopenhagenDenmark

Background:Determination of nutrient requirements in the late gestating and lactating sows is essential to optimize sow *** objectives of the present study were to quantify amino acid(AA)fluxes and heat production across portal-drained viscera(PDV)and liver in multiparous sows during transition and ***:Eight second parity sows were fitted with indwelling catheters in the femoral artery and in the mesenteric,portal and hepatic *** hourly sets of blood samples were taken starting 0.5 h before feeding at−10,−3,+3,and+17 d in milk(DIM).Blood gases,plasma metabolites and apparent total tract digestibility(ATTD)of nutrients were ***:Feed intake,the ATTD of DM,energy,nitrogen,fat and crude fiber changed with DIM(P<0.001).Except for Glu,O2,and urea,all net portal fluxes were positive,and all were affected by DIM(P<0.05)and by sampling time(P<0.01).Compared with pre partum levels,net portal uptake of AA was 3-63%lower at+3 DIM but 40-100%higher at+17 *** portal fluxes of AA peaked at 1.5 to 2.5 h after feeding except for Glu,and they were positively correlated with changes in sow feed intake across *** net portal recovery was low for Met(49%),Thr(54%),and His(54%)and high for the remaining essential AA(63-69%)and none of them differed across *** hepatic uptake(*** oxidation)of Lys,Thr,Ile,Leu and Phe peaked at 0.5 to 2.5 h after feeding,whereas uptake of Trp,Val,and His was constant,while that of Met was close to ***:The net portal recovery was substantially lower for Met,Thr,and His than the remaining essential *** AA oxidation peaks 0.5 to 2.5 h after *** heat production in PDV and liver was approximately two-fold higher at peak lactation compared to other *** study suggests that lysine was the limiting AA in peak lactation but not in early lactation.

关键词： Gestation Heat production Lactation Net hepatic flux Net portal flux Sow

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Metabolomic, Transcriptional, Hormonal, and Signaling Cross-Talk in Superroot2

引用

Molecular Plant 2010年第1期3卷 192-211页

作者： Marc Morant Claus Ekstrom peter Ulvskov Charlotte kristensen Mats Rudemo Carl Erik Olsen Jorgen Hansen Kirsten Jorgensena Bodil Jorgensen Birger Lindberg Moilera Soren Bak Plant Biochemistry Laboratory Department of Plant Biology and Biotechnology University of Copenhagen 40 Thorvaldsensvej DK-1871 FrederiksbergC Copenhagen Denmark Center for Molecular Plant Physiology University of Copenhagen. DK-1871 Frederiksberg C Copenhagen Denmark Department of Natural Sciences University of Copenhagen DK-1871 Frederiksberg C Copenhagen Denmark VKR research centre 'Pro-Active Plants' Plant Biochemistry Laboratory Department of Plant Biology and Biotechnology University of Copenhagen DK-1871 Frederiksberg C Copenhagen Denmark Evolva A/S Bulowsvej 25 DK-1870 Frederiksberg C Copenhagen Denmark Center for Applied Bioinformatics at LIFE University of Copenhagen 40 Thorvaldsensvej DK-1871 Frederiksberg C Copenhagen Denmark

Auxin homeostasis is pivotal for normal plant growth and development. The superroot2 （sur2） mutant was initially isolated in a forward genetic screen for auxin overproducers, and SUR2 was suggested to control auxin conju- gation and thereby regulate auxin homeostasis. However, the phenotype was not uniform and could not be described as a pure high auxin phenotype, indicating that knockout of CYP83B1 has multiple effects. Subsequently, SUR2 was identified as CYP83B1, a cytochrome P450 positioned at the metabolic branch point between auxin and indole glucosinolate metabolism. To investigate concomitant global alterations triggered by knockout of CYP83B1 and the countermeasures chosen by the mutant to cope with hormonal and metabolic imbalances, 10-day-old mutant seedlings were characterized with respect to their transcriptome and metabolome profiles. Here, we report a global analysis of the sur2 mutant by the use of a combined transcriptomic and metabolomic approach revealing pronounced effects on several metabolic grids including the intersection between secondary metabolism, cell wall turnover, hormone metabolism, and stress responses. Metabolic and transcriptional cross-talks in sur2 were found to be regulated by complex interactions between both positively and negatively acting transcription factors. The complex phenotype of sur2 may thus not only be assigned to elevated levels of auxin, but also to ethylene and abscisic acid responses as well as drought responses in the absence of a water deficiency. The delicate balance between these signals explains why minute changes in growth conditions may result in the non-uniform phenotype. The large phenotypic variation observed between and within the different surveys may be reconciled by the complex and intricate hormonal balances in sur2 seedlings decoded in this study.

关键词： Abiotic/environmental stress hormonal regulation metabolomics secondary metabolism/natural products transcriptome analysis development.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Tourniquets do not increase the total blood loss or reamputation risk in transtibial amputations

引用

World Journal of Orthopedics 2017年第1期8卷 62-67页

作者： Christian Wied peter T Tengberg Gitte Holm Thomas Kallemose Nicolai B Foss Anders Troelsen Morten T kristensen Depart-ment of Orthopedic Surgery Copenhagen University Hospital Hvidovre Clinical Research Centre Copenhagen University Hospital Hvidovre Department of Anesthesiology and Intensive Care Copenhagen University Hospital Hvidovre Physical Medicine and Rehabilita-tion Research--Copenhagen Department of Physical TherapyCopenhagen University Hospital Hvidovre

AIM To investigate the total blood loss(TBL) and the safety with respect to the re-amputation rate after transtibial amputation(TTA) conducted with and without a tourniquet. METHODS The study was a single-centre retrospective cohort study of patients with a primary TTA admitted between January 2013 and April 2015. All patients with a primary TTA were assessed for inclusion if the amputation was performed because of arteriosclerosis or diabetic complications. All patients underwent a standardized TTA procedure that was performed approximately 10 cm below the knee joint and performed with sagittalflaps. The pneumatic tourniquet, when used, was inflated around the femur to a pressure of 100 mmH g above the systolic blood pressure. The number of blood transfusions within the first four postoperative days was recorded. The intraoperative blood loss(OBL), which is defined as the volume of blood lost during surgery, was determined from the suction volume and by the weight difference of the surgical dressings. The trigger for a blood transfusion was set at a decrease in the Hgb level < 9.67 g/dL(6 mmol/L). Transfusions were performed with pooled red blood cells containing 245 m L per portion, which equals 55 g/L of haemoglobin. The TBL during the first four postoperative days was calculated based on the haemoglobin level and the estimated blood volume. The re-amputation rate was evaluated within 30 d. RESULTS Seventy-four out of 86 consecutive patients who underwent TTA within the two-year study period were included in the analysis. Of these, 38 were operated on using a tourniquet and 36 were operated on without using a tourniquet. There were no significant preoperative differences between the groups. The patients in both groups had a postoperative decrease in their Hgb level compared with preoperative baseline values. The patients operated on using a tourniquet received approximately three millilitres less blood transfusion per kilogram body weight compared with patients o

关键词： Total blood loss Intraoperative blood loss Transtibial amputation Lower extremity amputation Pneumatic tourniquet Re-amputation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：