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Chinese Medical Sciences Journal 2010年第1期25卷 1-12页

作者： Wen Zhang Xuan Zhang Xiao-li Wu Liu-sheng He Xiao-feng Zeng Amrie C. Grammer peter e. lipsky National Institute of Arthritis and Musculoskeletal and Skin Diseases Intramural Research Program National Institutes of Health Bethesda MD 20892 USA Department of Rheumatology Peking Union Medical College Hospital Chinese Academy of Medical Sciences ＆ Peking Union Medical College Beijing 100730 China

Objective To investigate the role of TNF receptor-associated factor 2 (TRAF-2) and TRAF6 in CD40-induced nuclear factor-κB (NF-κB) signaling pathway and whether CD40 signaling requires TRAF2. Methods Human B cell lines were transfected with plasmids expressing wild type TRAF2 or dominant negative TRAF2,TRAF2-shRNA,or TRAF6-shRNA. The activation of NF-κB was detected by Western blot,kinase assay,transfactor enzyme-linked immunosorbent assay (eLISA),and fluorescence resonance energy transfer (FReT). Analysis of the role of TRAF-2 and TRAF-6 in CD40-mediated NF-κB activity was examined following stimulation with recombinant CD154. Results TRAF2 induced activity of IκB-kinases (IKKα,IKKi/ε),phosphorylation of IκBα,as well as nuclear translocation and phosphorylation of p65/RelA. In contrast,TRAF6 strongly induced NF-κB activation and nuclear translocation of p65 as well as p50 and c-Rel. engagement of CD154-induced nuclear translocation of p65 was inhibited by a TRAF6-shRNA,but conversely was enhanced by a TRAF2-shRNA. examination of direct interactions between CD40 and TRAFs by FReT documented that both TRAF2 and TRAF6 directly interacted with CD40. However,the two TRAFs competed for CD40 binding. Conclusions These results indicate that TRAF2 can signal in human B cells,but it is not essential for CD40-mediated NF-κB activation. Moreover,TRAF2 can compete with TRAF6 for CD40 binding,and thereby limit the capacity of CD40 engagement to induce NF-κB activation.

关键词： human B lymphocytes TNF receptor-associated factor 2 TNF receptor-associated factor 6 IκB kinase IκBα p65

B淋巴细胞中CD154／CD40诱导转录因子核因子κB激活及在其系统性红斑狼疮发病机制中的研究

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B淋巴细胞中CD154／CD40诱导转录因子核因子κB激活及在其系统性...

TRAF是介导人类B细胞核转录因子κB活化的重要因子

中华医学会全国风湿病学年会

作者：张烜张奉春唐福林董怡 peter e lipsky Amrie C Grammer 中国医学科学院中国协和医科大学北京协和医院风湿免疫内科 Autoimmunity Branch NIAMSNIHBethesdaMDUSA 20892

目的:已知B淋巴细胞对免疫刺激的过度反应引发产生异常克隆细胞,分泌自身抗体,引发SLe。目前研究表明CD154／CD40的异常表达在SLe发病中有重要作用,而转录因子核因子κB(NF-κB)是淋巴细胞免疫反应极其重要调节因子,但CD154／CD40诱导NF...

目的:已知B淋巴细胞对免疫刺激的过度反应引发产生异常克隆细胞,分泌自身抗体,引发SLe。目前研究表明CD154／CD40的异常表达在SLe发病中有重要作用,而转录因子核因子κB(NF-κB)是淋巴细胞免疫反应极其重要调节因子,但CD154／CD40诱导NF-κB激活的具体机制还很不清楚,CD154／ CD40／NF-κB信号通路在SLe中的异常及其在SLe发病中的作用尚无文献报道。本研究将通过研究 CD154／CD40诱导NF-κB激活的具体机制,首次阐述其在SLe发病机制中的具体病理作用。前景是将来

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TRAF是介导人类B细胞核转录因子κB活化的重要因子

Metal-Organic Frameworks Housing Active Molecules as Bioinspired Catalysts

全国自身免疫性疾病专题研讨会暨第十一次全国风湿病学学术年会

作者：张文张烜何留胜 peter e．lipsky Amrie C．Grammer 北京协和医院风湿免疫科美国国立卫生研究院自身免疫病研究所

目的:TRAF2(肿瘤坏死因子受体相关因子2)是一种肿瘤坏死因子受体(如CD40)介导蛋白,传递 CD40的活化信号,对调节B细胞活化和分化功能起至关重要的作用。然而TRAF2如何调节CD40的核转录因子NF-κB信号通路还远不清楚。本研究将探讨TRAF2...

目的:TRAF2(肿瘤坏死因子受体相关因子2)是一种肿瘤坏死因子受体(如CD40)介导蛋白,传递 CD40的活化信号,对调节B细胞活化和分化功能起至关重要的作用。然而TRAF2如何调节CD40的核转录因子NF-κB信号通路还远不清楚。本研究将探讨TRAF2如何介导NF-κB通路的活化。方法:以人类B淋巴细胞株Ramos细胞为模型,转染野生型(WT-TRAF2)或功能区缺失型TRAF2

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CCS Chemistry 2024年第6期6卷 1380-1402页

作者： Yin Zhang peter e.VanNatta Junyu Ren Shengqian Ma Department of Chemistry University of North TexasDentonTexas 76201 Department of Chemical and Biomolecular engineering National University of SingaporeSingapore 117585

Optimization of activity,selectivity,and stability of catalysts for practical application is a long-soughtafter *** progress in the advancement of rational catalyst design routinely takes queues from ***,enzymes are renowned for their high efficiency and selectivity in diverse and significant reactions under mild *** the well-known merits of enzymes,they often remain unsuitable for industrial catalysis due to issues,including limited environment(solvent/temperature/pH)compatibility,considerable structural uncertainty,and highly specific reaction *** the context of bioinspired catalysis design,the development of synthetic heterogeneous catalyst analogs,which may not only mimic enzymes to reproduce or even surpass their performance but also bypass their intrinsic limitations,is thus,desirable and has been extensively studied in the context of porous *** metal-organic framework(MOF)is a superior candidate for porous materials towards this goal,thanks to facile and modular preparation,explicit crystalline composition/structure,tunable pore size/environment,and excellent *** introduction of active molecular species such as bioactive molecules(e.g.,proteins,enzymes,peptides,amino acids,porphyrins)and chemically active molecules(e.g.,frustrated Lewis pairs)into metal-organic frameworks(MOFs)via pore encapsulation,chemical postmodification,or bottom-up construction has been shown to facilitate the cooperative enhancement of catalysis through the enforcement of proximity,enhancement of control/activity,and even inducement of unexpected *** this minireview,we sought to summarize the last 5 years of fruitful progress,exploiting MOFs housing active molecules for substantial catalysis applications,including asymmetric reactions,CO_(2)conversion(reduction,cycloaddition),biocatalytic H_(2)O_(2)/dye degradation,and so on,and discuss challenges and opportunities in the prospective exploration of these system

关键词： metal-organic framework bioinspired catalyst active molecular species

experimental investigation on slip conditions during thread rolling with flat dies

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Friction 2024年第1期12卷 136-143页

作者： Stefan VOLZ peter GROCHe Institute for Production engineering and Forming Machines Technical University DarmstadtOtto-Berndt Straße 2Darmstadt 64287Germany

The process design of flat die rolling operations can be a tedious and costly process due to the challenges posed by small process *** this work,an effort is made to closely investigate and understand the process limits caused by excessive slippage of the *** measurements are carried out to capture the characteristics of excessive slippage and the influence of the stroke rate and the tribological system on this process *** differences in slippage were observed,depending on the tribological *** systems with polymer based lubricants showed excessive slippage at low stroke rates,systems using oil or no lubricant endured higher stroke rates before process limits were *** optical measurements revealed three major forms of slippage:successful rolling without slippage,failure due to excessive slippage at process start,and failure due to excessive slippage at around one workpiece rotation.

关键词： rolling incremental forming thread rolling slippage profile rolling

Ultrafast magneto-optical dynamics in nickel(111)single crystal studied by the integration of ultrafast reflectivity and polarimetry probes

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Chinese Physics B 2024年第3期33卷 65-69页

作者：匡皓余军潇陈洁 H.e.elsayed-Ali 李润泽 peter M.Rentzepis School of Physical Science and Technology ShanghaiTech UniversityShanghai 201210China Center for Transformative Science ShanghaiTech UniversityShanghai 201210China Center for Ultrafast Science and Technology Key Laboratory for Laser Plasmas (Ministry of Education)Collaborative Innovation Center of IFSA (CICIFSA)School of Physics and AstronomyShanghai Jiao Tong UniversityShanghai 200240China Department of electrical and Computer engineering Old Dominion UniversityNorfolkVirginia 23529USA Department of electrical and Computer engineering Texas A&M UniversityCollege StationTexas 77843USA

With the integration of ultrafast reflectivity and polarimetry probes,we observed carrier relaxation and spin dynamics induced by ultrafast laser excitation of Ni(111)single *** carrier relaxation time within the linear excitation range reveals that electron-phonon coupling and dissipation of photon energy into the bulk of the crystal take tens of *** the other hand,the observed spin dynamics indicate a longer time of about 120 *** further understand how the lattice degree of freedom is coupled with these dynamics may require the integration of an ultrafast diffraction probe.

关键词： ultrafast spin dynamics non-equilibrium dynamics multi-probe

Quantifying source-sink relationships in leaf-color modified rice genotypes during grain filling

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Journal of Integrative Agriculture 2024年第9期23卷 2923-2940页

作者： Zhenxiang Zhou Paul CStruik Junfei Gu peter e.L.van der Putten Zhiqin Wang Jianchang Yang Xinyou Yin Centre for Crop Systems Analysis Department of Plant SciencesWageningen University&ResearchWageningen 6700 AKThe Netherlands College of Agriculture Yangzhou UniversityYangzhou 225009China

Leaf-color modification can affect canopy photosynthesis,with potential effects on rice yield and yield *** source-sink relationships through crop management is often used to improve crop *** study investigated whether and how modifying leaf color alters source-sink relationships and whether current crop cultivation practices remain applicable for leaf-color modified *** collected data of total biomass and nitrogen(N)accumulation in rice genotypes of four genetic backgrounds and their leaf-color modified variants(greener or yellower)were analyzed,using a recently established modelling method to quantify the source-sink(im)balance during grain *** all leaf-color variants,only one yellower-leaf variant showed a higher source capacity than its normal *** was associated with greater post-flowering N-uptake that prolonged the functional leaf-N duration,and this greater post-flowering N-uptake was possible because of reduced pre-flowering N-uptake.A density experiment showed that current management practices(insufficient planting density accompanied by abundant N application)are unsuitable for the yellower-leaf genotype,ultimately limiting its yield ***-color modification affects source-sink relationships by regulating the N trade-off between pre-and post-flowering uptake,as well as N translocation between source and sink *** best exploit leaf-color modification for improving crop productivity,adjustments of crop management practices are required.

关键词： source-sink relationship biomass nitrogen Oryza sativa leaf-color modification

Identific tion of quantitative trait loci and associated candidate genes for pregnancy success in Angus–Brahman crossbred heifers

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Journal of Animal Science and Biotechnology 2024年第1期15卷 162-170页

作者： Quinn A.Hoorn Gabriel A.Zayas eduardo e.Rodriguez Laura M.Jensen Raluca G.Mateescu peter J.Hansen Department of Animal Sciences Donald Henry Barron Reproductive and Perinatal Biology Research Programand the Genetics InstituteUniversity of FloridaGainesvilleFLUSA School of Applied Systems Biology La Trobe UniversityBundooraVIC 3083Australia

Background In beef cattle, more than 50% of the energy input to produce a unit of beef is consumed by the female that produced the calf. Development of genomic tools to identify females with high genetic merit for reproductive function could increase the profitability and sustainability of beef *** Genome-wide association studies(GWAS) were performed using a single-step genomic best linear unbiased prediction approach on pregnancy outcome traits from a population of Angus–Brahman crossbred heifers. Furthermore, a validation GWAS was performed using data from another farm. Heifers were genotyped with the Bovine GGP F250 array that contains 221,077 SNPs. In the discovery population, heifers were bred in winter breeding seasons involving a single round of timed artificial insemination(AI) followed by natural mating for 3 months. Two phenotypes were analyzed: pregnancy outcome to first-service AI(PAI;n = 1,481) and pregnancy status at the end of the breeding season(PeBS;n = 1,725). The heritability was estimated as 0.149 and 0.122 for PAI and PeBS, respectively. In the PAI model, one quantitative trait locus(QTL), located between 52.3 and 52.5 Mb on BTA7, explained about 3% of the genetic variation, in a region containing a cluster of γ-protocadherin genes and SLC25A2. Other QTLs explaining between 0.5% and 1% of the genetic variation were found on BTA12 and 25. In the PeBS model, a large QTL on BTA7 was synonymous with the QTL for PAI, with minor QTLs located on BTA5, 9, 10, 11, 19, and 20. The validation population for pregnancy status at the end of the breeding season were Angus–Brahman crossbred heifers bred by natural mating. In concordance with the discovery population, the large QTL on BTA7 and QTLs on BTA10 and 12 were *** In summary, QTLs and candidate SNPs identified were associated with pregnancy outcomes in beef heifers, including a large QTL associated with a group of protocadherin genes. Confirmation of these associations

关键词： Beef cattle Fertility GWAS QTL

Towards implementation of alloy-specific thermo-fluid modelling for laser powder-bed fusion of Mg alloys

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Journal of Magnesium and Alloys 2024年第6期12卷 2327-2344页

作者： Mohammad Hoseini-Athar Mikael ersson peter Hedström Department of Material Science and engineering KTH Royal Institute of TechnologyStockholmSE-10044 Sweden

Multi-physics thermo-fluid modeling has been extensively used as an approach to understand melt pool dynamics and defect formation as well as optimizing the process-related parameters of laser powder-bed fusion(L-PBF).However,its capabilities for being implemented as a reliable tool for material design,where minor changes in material-related parameters must be accurately captured,is still in *** the present research,first,a thermo-fluid computational fluid dynamics(CFD)model is developed and validated against experimental *** the predicted material properties of the pure Mg and commercial ZK60 and We43 Mg alloys,parametric studies are done attempting to elucidate how the difference in some of the material properties,i.e.,saturated vapor pressure,viscosity,and solidification range,can influence the melt pool *** is found that a higher saturated vapor pressure,associated with the ZK60 alloy,leads to a deeper unstable keyhole,increasing the keyhole-induced porosity and evaporation mass *** viscosity and wider solidification range can increase the non-uniformity of temperature and velocity distribution on the keyhole walls,resulting in increased keyhole instability and formation of ***,the We43 alloy showed the best behavior in terms of defect formation and evaporation mass loss,providing theoretical support to the extensive use of this alloy in *** summary,this study suggests an approach to investigate the effect of materials-related parameters on L-PBF melting and solidification,which can be extremely helpful for future design of new alloys suitable for L-PBF.

关键词： Mg alloys Laser powder-bed fusion(L-PBF) Melt pool dynamics Computational fluid dynamics Fluid flow