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Antibacterial properties of lucifensin in Lucilia sericata maggots after septic injury

Asian Pacific Journal of Tropical Biomedicine 2014年第5期4卷 358-361页

作者： Ivana Valachova Emanuel Prochazka Jana Bohova Petr Novak peter takac Juraj Majtan Institute of Zoology Slovak Academy of Sciences Institute of Microbiology Academy of Sciences of the Czech Republic Scientica s.r.o.

Objective:To investigate the antibacterial properties of lucifensin in maggots of IjicUia sericala after septic ***:In our preliminary study we have shown that injuring the maggots with a needle soaked in lipopolysaccharide solution induced within 24 h lucifensin expression in the fat body and in the grease coupler of the salivary *** is assumed that lucifensin is secreted solely from this tissue into the haemoiymph(similar to other insect defensins) and not into secreted/excreted *** used high-performance liquid chromatography fractionation and radial diffusion assay to investigate the antibacterial properties of haemoiymph extracted from larvae after septic ***:After septic injury,production of lucifensin in the haemoiymph is *** led to higher antibacterial activity of such haemoiymph in comparison to non-stimulated ***:These results suggest that beside the previously demonstrated role of lucifensin in the debridement therapy,lucifensin is simultaneously important as a part of the systematic immune response.

关键词： Lucilia sericata Wound bacteria Defensin Lucifensin Immune-challenge

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The Welfare Assessment of Calves in Terms of the European Union Legislation

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Journal of Life Sciences 2012年第11期6卷 1299-1305页

作者： Jana takacova peter Korim Ladislav takac Jana Korimova JanaMal'ova Lukas Michalov Martin Bulik Institute of Judicial and Public Veterinary Medicine and Economics University of Veterinary Medicine and Pharmacy in Ko#iceKomensk~ho 73 Kogiee 04181 Slovak Republic

After entering to the Europe Union, Slovak Republic has implemented the EU legislation into the legislation of the Slovak Republic. The previous legislation of this country was effective in practice. So we were interested in, whether actual legislation is effective in practice too as previous or it will be necessary to make some changes in the legislation. This led us to carry out the assessment of welfare by farmed animal, specifically by calves. Our assessment was based on the Bristol Welfare Assurance Programme protocol developed at the University of Bristol. Very good results were achieved by assessment, and it shows about good level of legislation in the area of protection of animals, it has conditions and requirements, which will not require big changes and the breeder can ensure without bigger problems so to ensure to animals their five freedoms originally established by the Brambell Committee in 1965：（1） Freedom from hunger and thirst; （2） Freedom from discomfort; （3） Freedom fi＇om pain, injury or disease; （4） Freedom to express normal behaviour; （5） Freedom from fear and distress.

关键词： Calf welfare protection of animals legislation the European Union.

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