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Electrochemical Immunosensors for the Diagnosis of Celiac Disease

Advances in Chemical Engineering and Science 2015年第1期5卷 83-95页

作者： Katharina Anne Scherf peter koehler Herbert Wieser Deutsche Forschungsanstalt für Lebensmittelchemie Leibniz Institut Freising Germany

Celiac disease is a permanent intolerance to gluten proteins of wheat, rye, barley, and oats in genetically susceptible individuals. The clinical picture is characterized by inflammation and damage of the small intestinal mucosa and malabsorption of essential nutrients. Therapeutically, a lifelong strict gluten-free diet is necessary. The diagnosis of celiac disease is complex and includes symptomatology, serology, small intestinal histology, and genetic status. Serological testing plays a central role within the diagnostic procedure and is based on the measurement of disease-specific antibodies against gluten proteins (antigen) and tissue transglutaminase (autoantigen). Immunofluorescence detection and enzyme-linked immunosorbent assays are currently most often applied for antibody testing. However, these tests are expensive and time-consuming. Therefore, simple and rapid alternative methods have been developed during the last years, and electro-chemical immunosensors seem to be the most promising analytical tools. The architecture of these sensors may comprise the following elements: working and reference electrodes, covalent or noncovalent binding of the antigen to the surface of the working electrode by means of a functional monolayer, and blocking of unreacted binding sites. The analytical procedure is initiated by adding the analyte (serum antibodies) and an analyte-specific second antibody, which is usually labeled with an enzyme. The special reaction of the enzyme with an appropriate substrate results in a product that initiates a current that can be measured by different electrical methods. A number of different electrochemical immunosensors variable in different electrodes, binding systems, secondary antibodies, and current measurements have been developed. Most of them have been tested with real human serum samples of celiac patients and healthy individuals, and some of them reached disease sensitivity and specificity comparable with traditional analytica

关键词： Celiac Disease Diagnosis Electrochemical Immunosensors Serum Antibodies

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现代制造 2008年第35期 40-40页

作者： peter koehler

意大利滚筒式印染机床生产厂 Reggiani 公司为日本住友公司的新型 Fine-Cyclo 系列精密摆线减速机所折服,并在他们的新一代Dream 印染机中使用了这种减速机。

关键词：大功率摆线减速机质量日本住友公司 Dream ni公司印染机生产厂

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TRAIL-induced apoptosis of hepatocellular carcinoma cells is augmented by targeted therapies

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World Journal of Gastroenterology 2009年第47期15卷 5924-5935页

作者： Bruno Christian koehler Toni Urbanik Binje Vick Regina Johanna Boger Steffen Heeger peter R Galle Marcus Schuchmann Henning Schulze-Bergkamen First Department of Medicine Johannes Gutenberg-University Mainz55101 MainzGermany Steffen HeegerMerck KGaAMS-D Global Clinical Development Unit-Oncology64293 DarmstadtGermany National Center of Tumor Diseases Department of Medical OncologyUniversity Clinic of Heidelberg69120 HeidelbergGermany

AIM:To analyze the effect of chemotherapeutic drugs and specific kinase inhibitors,in combination with the death receptor ligand tumor necrosis factor-related apoptosis inducing ligand(TRAIL),on overcoming TRAIL resistance in hepatocellular carcinoma(HCC)and to study the efficacy of agonistic TRAIL antibodies,as well as the commitment of antiapoptotic BCL-2 proteins, in TRAIL-induced apoptosis. METHODS:Surface expression of TRAIL receptors (TRAIL-R1-4)and expression levels of the antiapoptotic BCL-2 proteins MCL-1 and BCL-xL were analyzed by flow cytometry and Western blotting,respectively. Knock-down of MCL-1 and BCL-xL was performed by transfecting specific small interfering *** cellswere treated with kinase inhibitors and chemotherapeutic *** induction and cell viability were analyzed via flow cytometry and 3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. RESULTS:TRAIL-R1 and-R2 were profoundly expressed on the HCC cell lines Huh7 and Hep-G2. However,treatment of Huh7 and Hep-G2 with TRAIL and agonistic antibodies only induced minor apoptosis *** resistance towards TRAIL could be considerably reduced by adding the chemotherapeutic drugs 5-fluorouracil and doxorubicin as well as the kinase inhibitors LY294002[inhibition of phosphoinositol- 3-kinase(PI3K)],AG1478(epidermal growth factor receptor kinase),PD98059(MEK1),rapamycin(mam- malian target of rapamycin)and the multi-kinase inhibitor ***,the antiapoptotic BCL-2 proteins MCL-1 and BCL-xL play a major role in TRAIL resistance:knock-down by RNA interference increased TRAIL-induced apoptosis of HCC ***, knock-down of MCL-1 and BCL-xL led to a significant sensitization of HCC cells towards inhibition of both c-Jun N-terminal kinase and ***:Our data identify the blockage of survival kinases,combination with chemotherapeutic drugs and targeting of antiapoptotic BCL-2 proteins as promising ways to overcome TRAIL resistance in

关键词： Hepatocellular carcinoma Apoptosis Tumor necrosis factor-related apoptosis inducing ligand BCL-xL MCL-1 5-fluorouracil Doxorubicin Sorafenib Phosphoinositol-3-kinase (Mitogen-activated protein kinase)/(extracellular signal regulated kinase) kinase c-Jun N-terminal kinase

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Localization of Active Caspase-3 and Caspase-8 in Nasal Polyps and Nasal Hyperplasia in Consideration of Mast Cell Function: A Semiquantitatively Analysis

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International Journal of Otolaryngology and Head & Neck Surgery 2012年第3期1卷 63-70页

作者： Nadine Franzke Sibylle koehler peter Middel Claudia Fuoco Francesco Cecconi Fabio Quondamatteo Rainer Laskawi Saskia Rohrbach Anatomy National University of Ireland Galway Ireland Department of Audiology and Phoniatrics Charite Medical University of Berlin Berlin Germany Department of Dermatology University Hamburg-Eppendorf Hamburg Germany Department of Histology University of Goettingen Goettingen Germany Department of Otorhinolaryngology University of Goettingen Goettingen Germany Dulbecco Telethon Institute Department of Biology University of Rome Tor Vergata Rome Italy Institute of Pathology Klinikum Kassel Kassel Germany

Introduction: The pathogenesis of nasal polyposis and nasal hyperplasia is still unknown. The localization of caspases in nasal polyps and nasal hyperplasia of patients with and without allergic rhinitis was studied. Methods: Sections of human nasal polyps (n = 5) and hyperplastic nasal turbinates (5 with, 5 without allergy) were stained for active caspase-3 and caspase-8. Double immunofluorescence was used to evaluate colocalization of the caspases with Ki-M1P and tryptase. TUNEL was performed. Results: Active caspase-3 and caspase-8 were seen in nearly all nasal polyps and hyperplastic nasal turbinates. Active caspase-3 was predominantly localized in stromal cells, identified as mast cells. Caspase-8 was localized in mast cells with the pattern similar to active caspase-3 and additionally found in epithelial cells at the nasal and polyp surface and in epithelial cells of glands. Conclusion: Our results suggest that mast cell apoptosis may be involved in the pathological mechanisms which characterize and sustain chronic inflammatory disorders of the nasal mucosa with and without allergy.

关键词： Nasal Polyposis Caspase-3 Caspase-8 Allergic Rhinitis Non-Allergic Rhinitis Mast Cells Nasal Pathology Chronic Rhinosinusitis Apoptosis Nasal Hyperplasia

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伴缓慢性心律失常的睡眠呼吸暂停综合征的发病率和病死率

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德国医学 1995年第2期12卷 122-124页

作者： U.koehler T.Wetzig J.H.peter T.Ploch H.Schfer M.Stellwaag 蒋学俊 Medizinische Poliklinik Zentrum Innere Medizin der UniversitatBaldingerstraβeD-35043 MarburgGermany 同济医科大学附属同济医院

睡眠呼吸暂停综合征患者,心血管疾病发病率和病死率较高,可与心功能不全、高血压、肺动脉高压及缓慢性心律失常同时出现。睡眠呼吸暂停综合征合并冠心病者可出现心肌缺血。很多学者提出,伴缓慢性心律失常者病死率较高。本研究回顾性分... 详细信息

睡眠呼吸暂停综合征患者,心血管疾病发病率和病死率较高,可与心功能不全、高血压、肺动脉高压及缓慢性心律失常同时出现。睡眠呼吸暂停综合征合并冠心病者可出现心肌缺血。很多学者提出,伴缓慢性心律失常者病死率较高。本研究回顾性分析了伴和不伴夜间缓慢性心律失常的睡眠呼吸暂停综合征患者的发病率和病死率。

关键词：睡眠性呼吸暂停综合征心律失常发病率病死率

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斯坦福大学露西尔帕卡德儿童医院

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当代建筑 2020年第3期 52-61页

作者：无 Rob Goodwin Robin Guenther Jason Allen Gerry Del Priore Nash Hurley Dutch Osborne Bay Chapman Breeze Glazer peter Syrett Cheryl Woo Annelise Chikhale Megan koehler Chris Swigert Tom Silva Hyeuk Rhee Vanna Conyers Suzanne Drake Tim Hernaez Angelene Hoffert Donna Sanderson Michael Tam Andy Tsay-Jacobs Kristian Fosholt Haji Ishikawa Vittoria Zupicich(摄) Emily Hagopian(摄) Steve Babuljak(摄) Tim Griffith(摄) 帕金斯威尔建筑设计事务所不详

既有的斯坦福大学露西尔帕卡德儿童医院建成于1990年,因标志性的设计和醒目的中央庭院而深受人们的喜爱。随着时间的推移,如今这座医院的承载容量已经无法满足不断增长的患者规模和当前复杂医疗运营模式的需要。半私密性病房再也无法达... 详细信息

既有的斯坦福大学露西尔帕卡德儿童医院建成于1990年,因标志性的设计和醒目的中央庭院而深受人们的喜爱。随着时间的推移,如今这座医院的承载容量已经无法满足不断增长的患者规模和当前复杂医疗运营模式的需要。半私密性病房再也无法达到预期的私密程度,也未能充分利用场地周边生机勃勃的自然资源环境,尤其是斯坦福植物园及远处连绵起伏的丘陵山貌。

关键词：儿童医院私密性自然资源环境斯坦福大学运营模式帕卡德连绵起伏中央庭院

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