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Functionalized scaffolds to enhance tissue regeneration

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Regenerative Biomaterials 2015年第1期2卷 47-57页

作者： Baolin Guo Bo Lei Peng Li peter x.ma Center for Biomedical Engineering and Regenerative Medicine Frontier Institute of Science and TechnologyXi’an Jiaotong UniversityXi’an 710049China Department of Biomedical Engineering University of Michigan Department of Biologic and materials Sciences University of Michigan1011North University AvenueRoom 2209 macromolecular Science and Engineering Center University of Michigan Department of materials Science and Engineering University of MichiganAnn ArborMI 48109USA

Tissue engineering scaffolds play a vital role in regenerative *** not only provides a temporary 3-dimensional support during tissue repair,but also regulates the cell behavior,such as cell adhesion,proliferation and *** this review,we summarize the development and trends of functional scaffolding biomaterials including electrically conducting hydrogels and nanocomposites of hydroxyapatite(HA)and bioactive glasses(BGs)with various biodegradable ***,the progress on the fabrication of biomimetic nanofibrous scaffolds from conducting polymers and composites of HA and BG via electrospinning,deposition and thermally induced phase separation is ***,bioactive molecules and surface properties of scaffolds are very important during tissue *** molecule-releasing scaffolds and antimicrobial surface coatings for biomedical implants and scaffolds are also reviewed.

关键词： biomaterials scaffolds electrically conductive polymers bioactive nanocomposites bone tissue engineering molecule-releasing scaffolds antimicrobial coatings

CHIP regulates bone mass by targeting multiple TRAF family members in bone marrow stromal cells

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Bone Research 2018年第2期6卷 145-154页

作者： Tingyu Wang Shan Li Dan Yi Guang-Qian Zhou Zhijie Chang peter x.ma Guozhi xiao Di Chen Department of Pharmacy Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine Department of Orthopedic Surgery Rush University Medical Center Department of Medical Cell Biology and Genetics Shenzhen Key Laboratory and the Center for Anti-Ageing and Regenerative Medicine Shenzhen University Medical School State Key Laboratory of Biomembrane and Membrane Biotechnology Tsinghua University School of Medicine Department of Biologic and materials Science University of Michigan

Carboxyl terminus of Hsp70-interacting protein（CHIP or STUB1） is an E3 ligase and regulates the stability of several proteins which are involved in different cellular functions. Our previous studies demonstrated that Chip deficient mice display bone loss phenotype due to increased osteoclast formation through enhancing TRAF6 activity in osteoclasts. In this study we provide novel evidence about the function of CHIP. We found that osteoblast differentiation and bone formation were also decreased in Chip KO mice. In bone marrow stromal（BMS） cells derived from Chip^-/- mice, expression of a panel of osteoblast marker genes was significantly decreased. ALP activity and mineralized bone matrix formation were also reduced in Chip-deficient BMS cells. We also found that in addition to the regulation of TRAF6, CHIP also inhibits TNFα-induced NF-κB signaling through promoting TRAF2 and TRAF5 degradation. Specific deletion of Chip in BMS cells downregulated expression of osteoblast marker genes which could be reversed by the addition of NF-κB inhibitor. These results demonstrate that the osteopenic phenotype observed in Chip^-/- mice was due to the combination of increased osteoclast formation and decreased osteoblast differentiation. Taken together, our findings indicate a significant role of CHIP in bone remodeling.

关键词： CHIP regulates bone mass targeting multiple TRAF family members marrow stromal cells

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Biomimetic polymer scaffolds for regeneration

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Biomimetic polymer scaffolds for regeneration

Biomimetic delivery of signals for bone tissue engineering

2007年全国高分子学术论文报告会

作者： peter x.ma

＜正＞Department of Biologic and materials Sciences,Department of Biomedical Engineering,macromolecular Science and Engineering Center,The University of Michigan,Ann Arbor,Michigan 48109-1078,USA（email:mapx@***）

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Bone Research 2018年第3期6卷 205-216页

作者： Ming Dang Laura Saunders xufeng Niu Yubo Fan peter x.ma macromolecular Science and Engineering Center University of Michigan Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education School of Biological Science and Medical Engineering Beihang University Beijing Advanced Innovation Center for Biomedical Engineering Beihang University Department of Biologic and materials Sciences University of Michigan Department of Biomedical Engineering University of Michigan Department of materials Science and Engineering University of Michigan

Bone tissue engineering is an exciting approach to directly repair bone defects or engineer bone tissue for *** play a pivotal role in providing a template and extracellular environment to support regenerative cells and promote tissue regeneration. A variety of signaling cues have been identified to regulate cellular activity, tissue development, and the healing process. Numerous studies and trials have shown the promise of tissue engineering, but successful translations of bone tissue engineering research into clinical applications have been limited, due in part to a lack of optimal delivery systems for these signals. Biomedical engineers are therefore highly motivated to develop biomimetic drug delivery systems, which benefit from mimicking signaling molecule release or presentation by the native extracellular matrix during development or the natural healing process. Engineered biomimetic drug delivery systems aim to provide control over the location, timing, and release kinetics of the signal molecules according to the drug's physiochemical properties and specific biological mechanisms. This article reviews biomimetic strategies in signaling delivery for bone tissue engineering, with a focus on delivery systems rather than specific molecules. Both fundamental considerations and specific design strategies are discussed with examples of recent research progress, demonstrating the significance and potential of biomimetic delivery systems for bone tissue engineering.

关键词： exciting approach therefore highly motivated

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人牙髓干细胞来源的外泌体在体外促进人牙髓干细胞分化及矿化

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人牙髓干细胞来源的外泌体在体外促进人牙髓干细胞分化及矿化

2019年中华口腔医学会老年口腔医学专业委员会第十四次全国老年口腔医学学术年会

作者：龚婷吴红崑楼北雁 peter x.ma 口腔疾病研究国家重点实验室国家口腔疾病临床医学研究中心四川大学华西口腔医院老年口腔科 Department of Biologic and materials Science School of DentistryUniversity of Michigan

目的:外泌体(Exosomes,Exos)是一种包含了复杂RNA和蛋白质的由细胞分泌的直径为50-150nm的盘状囊泡。由于外泌体可调节受体细胞相关生物活动,并且没有天然或组织工程干细胞移植的免疫排异反应,因而在组织再生领域逐渐受到重视。本研究... 详细信息

目的:外泌体(Exosomes,Exos)是一种包含了复杂RNA和蛋白质的由细胞分泌的直径为50-150nm的盘状囊泡。由于外泌体可调节受体细胞相关生物活动,并且没有天然或组织工程干细胞移植的免疫排异反应,因而在组织再生领域逐渐受到重视。本研究旨在探讨人牙髓干细胞(human Dental pulp stem cells,hDPSCs)外泌体在体外促进hDSPCs分化及矿化的作用。方法:hDPSCS在促矿化培养基(odontogenic media,OM)中培养14天后,从上清液中分离出外泌体,命名为OM-hDPSCExos。通过直径表征和特异性表面标记物证明提取的为外泌体。利用hDPSCs研究外泌体对其迁移、分化和矿化的影响。结果:茜素红染色显示,OM+OM-hDPSC-Exos处理后14天,基质矿化程度明显高于单纯OM处理。实时聚合酶链反应结果显示,在OM中添加OM-hDPSC-Exos 14天后,牙本质唾液磷蛋白(DSPP)和runt相关转录因子2 (RUNx2)表达上调。Transwell迁移实验表明,OM-hDPSCExos引起细胞迁移的多少与外泌体浓度成正比。结论:OM-hDPSC-Exos与OM协同诱导hDPSCs向成牙细胞分化,并在体外诱导矿化。来自hDPSCs的外泌体可能是一种很有前途的促进牙本质再生的工具。

关键词：外泌体人牙髓干细胞矿化

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纳米纤维微球促进成牙本质再生的基础研究

纳米纤维微球促进成牙本质再生的基础研究

Achieving strength–ductility synergy in novel paramagnetic Fe-based medium-entropy alloys through deep cryogenic deformation

第九次全国口腔材料学术交流会

作者：邝容张展鹏 peter x.ma 倪龙兴第四军医大学口腔医学院牙体牙髓病科

目的:牙髓牙本质再生一直是口腔医学的巨大挑战。本研究研发成功了一种纳米纤维微球,作为新型的可注射型细胞载体,研究了该材料对牙髓干细胞的增殖、分化的影响,进行了系列体外、体内实验,对促进牙本质再生应用的可能进行了评估。方法:... 详细信息

目的:牙髓牙本质再生一直是口腔医学的巨大挑战。本研究研发成功了一种纳米纤维微球,作为新型的可注射型细胞载体,研究了该材料对牙髓干细胞的增殖、分化的影响,进行了系列体外、体内实验,对促进牙本质再生应用的可能进行了评估。方法:合成了具有生物可降解的左旋聚乳酸共聚物,利用相分离技术合成了具有纳米纤维结构的微球体,用同样的原料合成了传统的实心光滑微球作为对照组。牙髓干细胞接种于微球表面并在生物反应器中培养,扫描电镜观察了细胞在微球表面生长形态,在培养1,3,5,7,9,12,14天用DNA定量的方法测定了细胞增殖曲线;在矿化诱导培养条件下,测试了3,7,14天细胞的碱性磷酸酶活性,实时定量PCR方法检测了2周和4周时成牙本质相关基因的表达。体内进行了裸鼠皮下注射细胞/微球复合体实验,术后6周取材进行了组织学检测。结果:纳米纤维微球和光滑实心微球具有同样直径范围,选用30-60微米直径的微球作为实验对象。纳米纤维微球由左旋聚乳酸纤维构成中空微球,与光滑实心微球相比,牙髓干细胞在纳米微球上更有立体感,有细胞突起,黏附数量多。DNA定量结果显示,14天内,培养在纳米纤维微球上的牙髓干细胞比在实心光滑微球上的增殖速度快。用成牙/成骨诱导液诱导培养7天、14天后,牙髓干细胞在纳米微球组的碱性磷酸酶活性明显强于实心光滑微球组;DSPP免疫荧光染色显示纳米纤维微球组染色阳性,而光滑实心微球组为阴性。皮下注射微球/细胞复合体6周后,纳米纤维微球组显示较好的成矿化组织的趋势,HE染色显示在纳米纤维微球组,新生组织生长良好,细胞密度大,微球载体有大量降解,组织有矿化,DSPP免疫组化染色阳性,而在光滑实心微球组的细胞生长增殖较弱,微球不降解,组织无矿化,DSPP染色阴性,展示了纳米纤维微球在成牙诱导方面的优势与未来巨大的应用潜能。结论:纳米纤维微球是一种很好的可注射性细胞载体,促进了牙髓干细胞增殖、成牙分化,为牙本质再生提供了良好的微环境。

关键词：纳米纤维微球牙髓干细胞牙本质组织工程

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Rare Metals 2024年第9期43卷 4493-4507页

作者： Hu-Wen ma Yan-Chun Zhao Li Feng Tian-Zeng Liu Zhi-Qi Yu Bo Jin Wang-Chun Duan peter K.Liaw Dong ma State Key Laboratory of Advanced Processing and Recycling of Non-Ferrous Metals Lanzhou University of Technology Wenzhou Pump and Valve Engineering Research Institute Lanzhou University of Technology Iron and Steel Research Institute Jiuquan Iron and Steel Corporation Lanzhou Resources and Environment Voc-Tech University Department of materials Science and Engineering The University of Tennessee Neutron Science Center Songshan Lake Materials Laboratory

Cryogenic pre-deformation treatment has been widely used to effectively improve the comprehensive mechanical properties of steels and novel metals. However,the dislocation evolution and phase transformation induced by different degrees of deep cryogenic deformation are not yet fully elucidated. In this study, the effects of multiple cryogenic pre-treatments on the mechanical properties and deformation mechanisms of a paramagnetic Fe63.3Mn14-Si9.1Cr9.8C3.8medium-entropy alloy（MEA） were investigated, leading to the discovery of a pretreated MEA that exhibits exceptional mechanical properties, including a fracture strength of 3.0 GPa, plastic strain of 26.1% and work-hardening index of 0.57. In addition, x-ray diffraction（xRD） and transmission electron microscopy（TEM）analyses revealed that multiple cryogenic pre-deformation treatments significantly increased the dislocation density of the MEA(from 9 × 1015to 4 × 1016m-2after three pretreatments), along with a transition in the dislocation type from predominantly edge dislocations to mixed dislocations（including screw-and edge-type dislocations）. Notably, this pretreated MEA retained its paramagnetic properties（μrx.mately four(i.e., from 9.7 to2.6 m J·m-2), thereby activating the synergistic effects of transformation-induced plasticity, twinning-induced plasticity and dislocation strengthening mechanisms. These synergistic effects lead to simultaneous strength and ductility enhancement of the MEA.

关键词： Deep cryogenic transformation Dislocation evolution Iron-based medium-entropy alloys Phase transformation Stacking fault energy

Metal-Organic Frameworks Housing Active Molecules as Bioinspired Catalysts

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CCS Chemistry 2024年第6期6卷 1380-1402页

作者： Yin Zhang peter E.VanNatta Junyu Ren Shengqian ma Department of Chemistry University of North TexasDentonTexas 76201 Department of Chemical and Biomolecular Engineering National University of SingaporeSingapore 117585

Optimization of activity,selectivity,and stability of catalysts for practical application is a long-soughtafter *** progress in the advancement of rational catalyst design routinely takes queues from ***,enzymes are renowned for their high efficiency and selectivity in diverse and significant reactions under mild *** the well-known merits of enzymes,they often remain unsuitable for industrial catalysis due to issues,including limited environment(solvent/temperature/pH)compatibility,considerable structural uncertainty,and highly specific reaction *** the context of bioinspired catalysis design,the development of synthetic heterogeneous catalyst analogs,which may not only mimic enzymes to reproduce or even surpass their performance but also bypass their intrinsic limitations,is thus,desirable and has been extensively studied in the context of porous *** metal-organic framework(MOF)is a superior candidate for porous materials towards this goal,thanks to facile and modular preparation,explicit crystalline composition/structure,tunable pore size/environment,and excellent *** introduction of active molecular species such as bioactive molecules(e.g.,proteins,enzymes,peptides,amino acids,porphyrins)and chemically active molecules(e.g.,frustrated Lewis pairs)into metal-organic frameworks(MOFs)via pore encapsulation,chemical postmodification,or bottom-up construction has been shown to facilitate the cooperative enhancement of catalysis through the enforcement of proximity,enhancement of control/activity,and even inducement of unexpected *** this minireview,we sought to summarize the last 5 years of fruitful progress,exploiting MOFs housing active molecules for substantial catalysis applications,including asymmetric reactions,CO_(2)conversion(reduction,cycloaddition),biocatalytic H_(2)O_(2)/dye degradation,and so on,and discuss challenges and opportunities in the prospective exploration of these system

关键词： metal-organic framework bioinspired catalyst active molecular species

Synthetic biodegradable functional polymers for tissue engineering:a brief review

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Science China Chemistry 2014年第4期57卷 490-500页

作者： GUO BaoLin ma peter x Center for Biomedical Engineering and Regenerative Medicine Frontier Institute of Science and Technology Xi'an Jiaotong University Department of Biomedical Engineering University of Michigan Department of Biologic and materials Sciences University of Michigan macromolecular Science and Engineering Center University of Michigan Department of materials Science and Engineering University of Michigan

Scaffolds play a crucial role in tissue engineering. Biodegradable polymers with great processing flexibility are the predominant scaffolding materials. Synthetic biodegradable polymers with well-defined structure and without immunological concerns associated with naturally derived polymers are widely used in tissue engineering. The synthetic biodegradable polymers that are widely used in tissue engineering, including polyesters, polyanhydrides, polyphosphazenes, polyurethane, and poly(glycerol sebacate) are summarized in this article. New developments in conducting polymers, photoresponsive polymers, amino-acid-based polymers, enzymatically degradable polymers, and peptide-activated polymers are also discussed. In addition to chemical functionalization, the scaffold designs that mimic the nano and micro features of the extracellular matrix(ECM) are presented as well, and composite and nanocomposite scaffolds are also reviewed.

关键词： synthetic biodegradable polymers functional polymers scaffolds tissue engineering

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