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Detection of class 1 integron in Acinetobacter baumannii isolates collected from nine hospitals in Turkey

Asian Pacific Journal of Tropical Biomedicine 2013年第9期3卷 743-747页

作者： Aysegül Copur Cicek Azer Ozad Düzgün Aysegül Saral Tuba Kayman Zeynep Cizmeci pervin ozlem balci Tuba Dal Mehmet Firat Ismail Tosun Yasemin Ay Alitntop Ahmet Caliskan Yelda Yazici Cemal Sandalli Department of Medical Microbiology Faculty of MedicineRecep Tayyip Erdogan University Department of Genetics and Bioengineering Faculty of Engineering and Natural SciencesGümüshane University Department of Biology Faculty of Arts and SciencesArtvin Coruh University Clinical Microbiology Laboratory Research and Training Hospital Clinical Microbiology Laboratory Kecioren Research and Training Hospital Clinical Microbiology Laboratory General State Hospital Department of Medical Microbiology Faculty of MedicineDicle University Clinic of Infectious Diseases and Clinical Microbiology PrivateOSM Ortadogu Hospital Microbiology Laboratory Kirikkale Highly Specialized Hospital General State Hospital Microbiology Laboratory Necip Fazil City Hospital Clinical Microbiology Laboratory Ahi Evren Chest and Cardiovascular Surgery Research and Training Hospital Department of Biology Faculty of Arts and SciencesRecep Tayyip Erdogan University

Objective:To investigate the antibiotic resistance genes inserted into class I and class 2integrons in Acinetobacler baumannii[***)isolates obtained from nine different cities in ***:A collection of 281 *** clinical isolates were collected from nine diferenl state hospitals in Turkey and were confirmed as *** by conventional biochemical,API testing and bla_(-OXA-51)specific *** isolates were examined by PCR for existence of class I and2 integron gene ***:They were characterized by antimicrobial susceptibility testing and the highest resistance rates were determined for piperacillin(90.03%),ciprofloxacin(87.54%),cefepime and trimethoprim/sulfamethoxazole(81.13%).The lowest resistance rates was for cefotaxime(3.55%).class 1 integrons were detected in 6.4%(18/281)of *** strains and no class 2 integron was *** gene casselles of class 1 inlegrons AacCI-AAC(3)l-aadAI,AacCI-aadA1,AAC(3)-I,AAC(3)-I-AAC(3)-I-aadA1,***(3)-I-aadA1-AAC(3)-I-AAC(3)-I,AAC(3)-I-AAC(3)-I-AAC(3)-I-aadA1,AAC(3)-I-aadA1,AAC(3)-I-AAC(3)-I,AAC(3)-I-aadA1-AAC(3)-I-aadA1,AAC(3)-I-AAC(3)-I-aadA1-AAC(3)-I-aadA1 were detected in eighteen *** aac genes family were most frequently found integrated into the class 1 inlegrons and it was followed by aadA genes and TEM-1 ***:This is an extensive study on the distribulion of class 1 integron among *** in *** addition to these,two new alleles were *** percentage rates of similarity to other cassettes are 95%aadA1(TK A18)and 89%,aadA 1(ANKA3).

关键词： Acinetobacter baumannii Class 1 integron Gene cassette Resistance

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Screening of Class 1 and Class 2 Integrons in Clinical Isolates of Pseudomonas aeruginosa Collected from Seven Hospitals in Turkey:A Multicenter Study

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Open Journal of Medical Microbiology 2013年第4期3卷 227-233页

作者： Aysegul Copur Cicek Aysegul Saral Azer Ozad Duzgun Zeynep Cizmeci Tuba Kayman pervin ozlem balci Tuba Dal Mehmet Firat Yelda Yazici Metin Sancaktar Osman Birol Ozgumus Cemal Sandalli Department of Medical Microbiology Faculty of MedicineRecep Tayyip Erdogan UniversityRizeTurkey Department of Biology Faculty of Arts and SciencesArtvin Coruh UniversityArtvinTurkey Department of Genetics and Bioengineering Faculty of Engineering and Natural SciencesGumushane UniversityGumushaneTurkey Clinical Microbiology Laboratory Kecioren Research and Training HospitalAnkaraTurkey Clinical Microbiology Laboratory Research and Training HospitalKayseriTurkey Clinical Microbiology Laboratory General State HospitalTokatTurkey Department of Medical Microbiology Faculty of MedicineDicle UniversityDiyarbakirTurkey Clinic of Infectious Diseases and Clinical Microbiology Private OSM Ortadogu HospitalSanliurfaTurkey Clinical Microbiology Laboratory Ahi Evren Chest and Cardiovascular Surgery Research and Training HospitalTrabzonTurkey Clinical Microbiology Laboratory Akcaabat Hackali Baba General State HospitalTrabzonTurkey Department of Biology Faculty of Arts and SciencesRecep Tayyip Erdogan UniversityRizeTurkey

Pseudomonas aeruginosa is one of the leading nosocomial pathogens worldwide, and their infections are difficult to treat due to acquired resistance to many antibiotics. This study aimed to detect class 1 and 2 integrons and antibiotic susceptibility of clinical isolates of P. aeruginosa. Two hundred and five P. aeruginosa strains were collected from the seven general state hospitals in Turkey. They were characterized by antimicrobial susceptibility testing, screened for class 1 and class 2 integrons, and evaluated for the association between antibiotic resistance phenotypes and the presence of integrons. intI gene was amplified in 10 isolates (4.87%) by PCR and in seven isolates of them (70%) were found different gene cassettes. The aadA gene integrated into the class 1 integrons was most frequently found and it was followed by aac genes and blaOXA family genes. Sequence analysis of variable regions of the class 1 integrons showed five gene cassette arrays;aadA1(99%), aac(3)-Id(82%)-orf-aac(3”)-Ia(99%), aac(3)-Ie(83%)-blaoxa10(100%)- aadA1 (100%), aadA6(99%, 100%), aac(6’)-I(97%)-orf-aadA2(99%). No class 2 integron was detected. This study is the first multicenter study for class 1 integrons and it indicates the low rate of presence of class 1 gene cassette in P. aeruginosa.

关键词： Pseudomonas aeruginosa Antibiotic Resistance Class 1 Integron AadA Multicenter Study

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