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Development of a quantum-dot-labelled magnetic immunoassay method for circulating colorectal cancer cell detection

World Journal of Gastroenterology 2012年第32期18卷 4419-4426页

作者： Maria Gazouli Anna Lyberopoulou pericles pericleous Spyros Rizos Gerassimos Aravantinos Nikolaos Nikiteas Nicholas P Anagnou Efstathios P Efstathopoulos Department of Basic Medical Sciences Laboratory of BiologySchool of MedicineUniversity of Athens11527 AthensGreece First Department of Surgery "Tzaneion" General Hospital18536 PiraeusGreece Third Department of Internal Medicine Agii Anargyri Hospital14564 AthensGreece Second Department of Propedeutic Surgery University of Athens School of MedicineLaiko General Hospital11527 AthensGreece Department of Radiology Attikon University Hospital12462 AthensGreece

AIM:To detect of colorectal cancer(CRC) circulating tumour cells(CTCs) surface antigens,we present an assay incorporating cadmium selenide quantum dots(QDs) in these ***:The principle of the assay is the immunomagnetic separation of CTCs from body fluids in conjunction with QDs,using specific antibody biomarkers:epithelial cell adhesion molecule antibody,and monoclonal cytokeratin 19 *** detection signal was acquired from the fluorescence signal of *** the evaluation of the performance,the method under study was used to isolate the human colon adenocarcinoma cell line(DLD-1) and CTCs from CRC patients' peripheral ***:The minimum detection limit of the assay was defined to 10 DLD-1 CRC cells/mL as fluorescence was measured with a *** cell sorting analysis and Real Time RT-PCR,they both have also been used to evaluate the performance of the described *** conclusion,we developed a simple,sensitive,efficient and of lower cost(than the existing ones) method for the detection of CRC CTCs in human *** have accomplished these results by using magnetic bead isolation and subsequent QD fluorescence ***:The method described here can be easily adjusted for any other protein target of either the CTC or the host.

关键词： Circulating tumor cells Cancer Quantum dots Nanoprobes Micrometastasis

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