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Lipocalin-2-Mediated Insufficient Oligodendrocyte Progenitor Cell Remyelination for White Matter Injury After Subarachnoid Hemorrhage via SCL22A17 Receptor/Early Growth Response Protein 1 Signaling

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Neuroscience Bulletin 2022年第12期38卷 1457-1475页

作者： Qiang Li Xufang Ru Yang Yang Hengli Zhao Jie Qu Weixiang Chen pengyu pan Huaizhen Ruan Chaojun Li Yujie Chen Hua Feng Department of Neurosurgery and State Key Laboratory of Trauma Burn and Combined InjurySouthwest HospitalThird Military Medical University(Army Medical University)Chongqing 400038China Department of Neurobiology College of Basic Medical SciencesThird Military Medical University(Army Medical University)Chongqing 400038China Model Animal Research Center Nanjing UniversityNanjing 210032China Chongqing Key Laboratory of Precision Neuromedicine and Neuroregenaration Southwest HospitalThird Military Medical University(Army Medical University)Chongqing 400038China Chongqing Clinical Research Center for Neurosurgery Southwest HospitalThird Military Medical University(Army Medical University)Chongqing 400038China

Insufficient remyelination due to impaired oligodendrocyte precursor cell(OPC)differentiation and maturation is strongly associated with irreversible white matter injury(WMI)and neurological deficits.We analyzed whole transcriptome expression to elucidate the potential role and underlying mechanism of action of lipocalin-2(LCN2)in OPC differentiation and WMI and identified the receptor SCL22A17 and downstream transcription factor early growth response protein 1(EGR1)as the key signals contributing to LCN2-mediated insufficient OPC remyelination.In LCN-knockdown and OPC EGR1 conditional-knockout mice,we discovered enhanced OPC differentiation in developing and injured white matter(WM);consistent with this,the specific inactivation of LCN2/SCl22A17/EGR1 signaling promoted remyelination and neurological recovery in both atypical,acute WMI due to subarachnoid hemorrhage and typical,chronic WMI due to multiple sclerosis.This potentially represents a novel strategy to enhance differentiation and remyelination in patients with white matter injury.

关键词： White matter injury Oligodendrocyte progenitor cell Remyelination Subarachnoid hemorrhage Multiple sclerosis Lipocalin-2 Early growth response protein 1

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桥墩偏心情况下转体球铰结构受力分析及配筋设计

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铁道勘测与设计 2024年第1期 34-39页

作者：潘鹏宇中铁第四勘察设计院集团有限公司桥梁院武汉430063

曲线桥梁转体施工过程中因横向偏心产生不平衡弯矩,设计时需将桥墩相对于转盘进行偏心设置,现有对桥墩偏心情况下的转体结构研究较少,为研究桥墩偏心这一特殊情况对转盘受力的影响,提出更合理的配筋设计方案,以广东省某T构转体桥为背景... 详细信息

曲线桥梁转体施工过程中因横向偏心产生不平衡弯矩,设计时需将桥墩相对于转盘进行偏心设置,现有对桥墩偏心情况下的转体结构研究较少,为研究桥墩偏心这一特殊情况对转盘受力的影响,提出更合理的配筋设计方案,以广东省某T构转体桥为背景进行研究。基于Midas Fea平台,建立球铰实体有限元模型,通过桥梁纵向计算得到桥墩墩顶竖向反力、不平衡弯矩,分析球铰结构各施工阶段中的受力分布规律,然后进行配筋设计,通过对比不同预应力钢束参数下球铰不同施工阶段的受力情况,得到预应力钢束对球铰受力影响规律,提出曲线桥梁转体结构配束设计的一般性建议。结果表明:对于双肢薄壁墩,上转盘顶面中心区域主拉应力、拉应力最大,配筋设计时作为主要控制因素;桥墩存在横向偏心,导致横向正应力极值点向偏心方向移动;配置预应力钢束时应关注正应力较大的方向,采用对称配束。

关键词：偏心转体施工球铰实体分析配筋设计

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基于多电平滞环电流控制的牵引供电系统可控宽频带阻抗测量技术

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电工技术学报 2024年第9期39卷 2771-2783页

作者：陈司懿胡海涛肖冬华潘鹏宇宋依桐西南交通大学电气工程学院成都611756 国网四川省电力公司仁寿县供电分公司眉山620500 国网四川省电力公司电力科学研究院成都610072

精确获取牵引供电系统的宽频带阻抗特性,是分析车-网耦合系统的宽频带振荡问题的关键途径,对保障电气化铁路的安全稳定运行具有重要意义。目前的阻抗测量技术大多难以兼顾大功率与频带可控的测量要求,因此,该文提出一种基于多电平滞环... 详细信息

精确获取牵引供电系统的宽频带阻抗特性,是分析车-网耦合系统的宽频带振荡问题的关键途径,对保障电气化铁路的安全稳定运行具有重要意义。目前的阻抗测量技术大多难以兼顾大功率与频带可控的测量要求,因此,该文提出一种基于多电平滞环电流控制的牵引供电系统可控宽频带阻抗测量技术,测量频带为10 Hz~5 kHz,可通过一次谐波扰动注入快速获取目标频带的系统阻抗,相比于传统的滞环电流控制方法,具有更低的开关频率,并且能有效避免死区效应,改善输出电流的波形,更适用于宽频信号的跟踪控制。最后,通过仿真和硬件在环实验验证了该测量技术的有效性。

关键词：牵引供电系统阻抗测量技术多电平滞环电流控制谐波扰动

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Aggravated pulmonary injury after subarachnoid hemorrhage in PDGF-B^ret/ret mice

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Chinese Neurosurgical Journal 2020年第3期6卷 152-160页

作者： pengyu pan Jie Qu Qiang Li Rongwei Li Yang Yang Shilun Zuo Xin Liu Hua Feng Yujie Chen Department of Neurosurgery Southwest HospitalThird Military Medical UniversityChongqing 400038China Department of Neurosurgery General Hospital of Shenyang Military CommandShenyang 110016China State Key Laboratory of Trauma Burn and Combined InjuryThird Military Medical UniversityChongqing 400038China Chongqing Key Laboratory of Precision Neuromedicine and Neuroregenaration Southwest HospitalThird Medical UniversityChongqing 400038China

Background:Recent advances in surgical and neuroprotective strategies could effectively manage the pathophysiological progression of subarachnoid hemorrhage(SAH).However,pulmonary dysfunction frequently occurs in SAH patients with an increased risk of unsatisfactory outcomes.Based on the similar microvascular structures in the blood-air barrier and blood-brain barrier and possible brain-lung crosstalks,we believe that pericytes may be involved in both neurological and pulmonary dysfunction after SAH.Methods:In our experiments,platelet-derived growth factor B(PDGF-B)retention motif knockout(PDGF-Bret/ret)mice and adeno-associated virus PDGF-B were employed to show the involvement of pericyte deficiency and PDGF-B expression.Neurological score,SAH grade,hematoxylin-eosin staining,and PaO2/FiO2 ratio analysis were performed to evaluate the neurological deficits and pulmonary functions in endovascular perforation SAH models at 24 h after surgery,as well as western blotting and immunofluorescence staining for underlying molecular expressions.Results:We found that neonatal PDGF-Bret/ret mice exhibited pulmonary atelectasis 12 h after birth.Further investigation showed a decrease in PaO2/FiO2 and lung-specific surfactant proteins in adult PDGF-Bret/ret mice.These dysfunctions were much worse than those in wild-type mice at 24 h after SAH.PDGF-B overexpression alleviated pulmonary dysfunction after SAH.Conclusions:These results suggested pulmonary dysfunction after SAH and the pivotal role of PDGF-B signaling for the pathophysiological process and future therapeutic targets of pulmonary injury treatment after SAH.Further studies are needed for pathophysiological investigations and translational studies on pulmonary injuries after SAH.

关键词： Platelet-derived growth factor B Pulmonary injury Subarachnoid hemorrhage Lung-specific surfactant protein

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全功率变速抽水蓄能机组水泵模式建模及运行特性研究

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四川电力技术 2024年第3期47卷 13-16,80页

作者：潘鹏宇史华勃陈刚丁理杰国网四川省电力公司电力科学研究院四川成都610041 电力物联网四川省重点实验室四川成都610041

全功率变速抽水蓄能机组(FSC-VSPSU)能够为电网提供快速、灵活的调节容量,助力新能源为主体的新型电力系统快速发展,其应用逐渐广泛。为掌握实际FSC-VSPSU在水泵工作模式(抽水)时的运行特性,以及分析工程现场所出现的实际问题,建立了FSC... 详细信息

全功率变速抽水蓄能机组(FSC-VSPSU)能够为电网提供快速、灵活的调节容量,助力新能源为主体的新型电力系统快速发展,其应用逐渐广泛。为掌握实际FSC-VSPSU在水泵工作模式(抽水)时的运行特性,以及分析工程现场所出现的实际问题,建立了FSC-VSPSU水泵模式下的仿真模型。此外,FSC-VSPSU在水泵模式时通常采用直接开度控制方法,对抽水功率无法精确控制,通过外加功率控制环,就能够很好地控制抽水功率,可补充作为机组的另一种控制方法。通过仿真FSC-VSPSU在直接开度控制以及外加功率控制环两种方式下的运行,总结了其运行特性,为实际FSC-VSPSU现场运行提供仿真工具以及运行调试指导。

关键词：全功率变速抽水蓄能机组水泵模式仿真建模运行特性

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以利伐沙班干混悬剂为例分析模型引导的药物研发在儿科药物开发中的应用

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中国医药工业杂志 2022年第11期53卷 1622-1627页

作者：潘鹏玉韩鸿璨车津晶王骏国家药品监督管理局药品审评中心北京100022 军事医学研究院毒物药物研究所北京100850

利伐沙班干混悬剂于2021年被美国FDA批准用于至18岁以下患者,在至少5 d的初始肠外抗凝治疗后,使用利伐沙班治疗静脉血栓栓塞(VTE)及降低复发性VTE的风险。利伐沙班干混悬剂作为儿科药物开发时是基于成人临床研究数据,采用模型引导的药... 详细信息

利伐沙班干混悬剂于2021年被美国FDA批准用于至18岁以下患者,在至少5 d的初始肠外抗凝治疗后,使用利伐沙班治疗静脉血栓栓塞(VTE)及降低复发性VTE的风险。利伐沙班干混悬剂作为儿科药物开发时是基于成人临床研究数据,采用模型引导的药物研发方式对儿科临床试验中的初始剂量选择、Ⅱ期和Ⅲ期临床试验的剂量调整提供支持。本研究分析了利伐沙班干混悬剂儿科研究的相关内容和FDA的评价考虑,讨论模型引导的药物研发在儿科药物开发中的作用。

关键词：利伐沙班干混悬剂儿科药物剂量选择模型引导的药物研发

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Aggravated Pulmonary Injury after Subarachnoid Hemorrhage in PDGF-Bret/ret Mice

Aggravated Pulmonary Injury after Subarachnoid Hemorrhage in...

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第十四届中国医师协会神经外科医师年会

作者： pengyu pan Jie Qu Qiang Li Rongwei Li Yang Yang Shilun Zuo Xin Liu Hua Feng Yujie Chen Department of Neurosurgery General hospital of Northern theater command

Background: Recent advances in surgical and neuroprotective strategies could effectively manage the pathophysiological progresses of SAH. However, pulmonary dysfunction frequently occurred in SAH patients with an increased risk of unsatisfied outcomes. Methods: In the present study, PDGF-B retention motif knockout mice(PDGF-B) and adeno-associated virus PDGF-B were employed. Neurological score, SAH grade, HE staining, PaO/FiO ratio, Western blotting and Immunofluorescence staining were performed to evaluate the neurological deficits and pulmonary functions in endovascular perforation SAH models. Results: We found neonatal PDGF-B mice exhibited pulmonary atelectasis at 12-hours after birth. Further investigation showed decreasing in PaO/FiO and lung-specific surfactant proteins in adult PDGF-B mice. These dysfunctions were much worse than wildtypes at 24-hours after SAH. And PDGF-B overexpressing could alleviate the pulmonary dysfunctions after SAH. Conclusions: These results suggested pulmonary dysfunction after SAH, as well as involvement of PDGF-B signal for the pathophysiological process and future therapeutic targets of the pulmonary injury treatment after SAH. Further studies are still needed for the pathophysiology investigations and translational researches on the pulmonary injuries after SAH.

关键词： Subarachnoid hemorrhage Pulmonary injury Platelet-derived growth factor B lung-specific surfactant protein

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MAP4K4 Induced Rapid Blood-Brain Barrier Damage after Subarachnoid Hemorrhage in Mice

MAP4K4 Induced Rapid Blood-Brain Barrier Damage after Subara...

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第十四届中国医师协会神经外科医师年会

作者： Zheng Zou pengyu pan Dongdong Liu Gen Li Guangzhi Hao Xu Gao Hua feng Yushu Dong Guobiao Liang Department of Neurosurgery General Hospital of Shenyang Military CommandThe Graduate Training Base of Liaoning Medical College Department of Neurosurgery General hospital of Shenyang Military Command Dalian Medical University Department of Neurosurgery Southwest HospitalThird Military Medical University Army Medical university

Subarachnoid hemorrhage is a catastrophic disease with high morbidity and mortality. Sterile-20-like mitogen-activated protein kinase kinase kinase kinase 4（MAP4K4） was found to be expressed in endothelial cell（EC） and previous studies showed MAP4K4 was involved in vascular damage. To investigate whether MAP4K4 play a role in pathophysiology of subarachnoid hemorrhage, we evaluated time course expression and endothelial cell expression of MAP4K4 in ipsilateral brain post SAH. SAH model was established by endovascular puncture with mice, intracerebroventricular injection was used to delivery MAP4K4 recombinant protein, scramble small interfering RNA and MAP4K4 small interfering RNA. PF-06260933, a small molecule inhibitor of MAP4K4 was administrated orally. Neurological score, brain water content, Evans blue extravasation, immunofluorescence, western blot, and gelatinase zymography were utilized for analyzing neurological outcome and mechanism of MAP4K4 and downstream NF-κB. MAP4K4 elevated in cortex of left/ipsilateral hemisphere and peak at 24 h after SAH and was colocalized with endothelial cells. MAP4K4 recombinant protein infusion aggravated neurological impairment, brain edema, BBB damage through activating NF-κB signal which inducing MMP9 maturation, while MAP4K4 small interfering RNA injection reversed those effects. Taken together, these results reveal that MAP4K4 is a key factor that promoting BBB disruption after SAH via activating NF-κB then enhancing MMP9 expression. PF-06260933 10 mg/kg protected BBB and neurological function by reducing NF-κB activation and MMP expression which degraded tight junction. Above results provided evidence supporting to target MAP4K4 after SAH.

关键词： subarachnoid hemorrhage blood-brain barrier vascular inflammation Sterile-20-like mitogen-activated protein kinase kinase kinase kinase 4 early brain injury

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多室箱梁桥横隔梁的拉压杆模型及其自动实现

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东南大学学报:自然科学版 2023年第5期53卷 886-895页

作者：宋旭明孙凯潘鹏宇唐冕郑梦洋中南大学土木工程学院长沙410075 中铁第四勘察设计院集团有限公司武汉430063

为解决现有规范中单箱多室箱梁支点横隔梁缺乏系统的拉压杆模型建立方法的问题,首先利用拓扑优化功能得到横隔梁拉压杆模型的初步构形,然后根据应力迹线图对模型中的杆件进行补充修正,得到合理的拉压杆模型.研究了支座间距、支座宽度、... 详细信息

为解决现有规范中单箱多室箱梁支点横隔梁缺乏系统的拉压杆模型建立方法的问题,首先利用拓扑优化功能得到横隔梁拉压杆模型的初步构形,然后根据应力迹线图对模型中的杆件进行补充修正,得到合理的拉压杆模型.研究了支座间距、支座宽度、横隔梁宽高比等参数对拉压杆模型的影响,根据支座间距和横隔梁宽高比将单箱三室支点横隔梁的拉压杆模型分为3类,并采用遗传算法实现该3类拉压杆模型的自动构建.结果表明,自动构建方法生成的拉压杆模型与有限元应力积分的计算结果比值在0.95~1.35之间,可以满足支点横隔梁的设计要求.所提出的方法可以实现自动构建多室箱梁横隔梁的拉压杆模型,对支点横隔梁的受力分析及设计计算具有一定的实用价值.

关键词：横隔梁拉压杆模型拓扑优化应力迹线遗传算法自动实现

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Pericyte-dependent suppression of transcytosis in experimental subarachnoid hemorrhage induced blood brain barrier leakage

Pericyte-dependent suppression of transcytosis in experiment...

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第十四届中国医师协会神经外科医师年会

作者： Qiang Li Xufang Ru pengyu pan Jie Qu Hengli Zhao Shilun Zuo Yujie Chen John H Zhang Hua Feng Huaizhen Ruan Department of Neurobiology College of Basic Medical SciencesThird Military Medical University (Army Medical University) Department of Neurosurgery Southwest HospitalThird Military Medical University (Army Medical University) Department of Physiology and Pharmacology Loma Linda University

Objective: Suppression of transcytosis is required for blood brain barrier(BBB) maintenance, and pericyte is potential regulating the BBB by inhibiting transcytosis. Therefore, Clarify and intervention to transcytosis induced BBB leakage after subarachnoid hemorrhage(SAH) are of prime interest. Our main aim was to study the potential role of pericyte foxf2 in transcytosis and consequent BBB disruption after SAH. Approach and Results: In an endovascular perforation SAH model, we characterize the SAH-induced BBB leakage in wide type(WT) and pericyte knockout(p) mice and subsequent brain edema on a cellular, molecular, and functional level. Pericyte paucity aggravated the reduction of capillary foxf2 and docosahexaenoic acid(DHA) levels, endothelial transcytosis and BBB leakage after SAH;Using both retroviral and adenovirus associated viral approaches, we show that genetic inhibition of foxf2 significantly reduced the production of DHA from cultured pericyte;In contrast, overexpression of foxf2 enriched capillary DHA concentration, reduce cav1 and transcytosis, and eventually alleviated BBB disruption, brain edema and neurological defect at 2d after SAH. Conclusions: SAH causes pericyte reduce the production of DHA thereby enhances transcytosis and BBB disruption. Foxf2 plays an important role in those pathologies and could be a therapeutic target for SAH-induced BBB leakage.

关键词： blood–brain barrier pericyte foxf2 transcytosis subarachnoid hemorrhage

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