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Transcriptomic analysis reveals hub genes and pathways in response to acetic acid stress in Kluyveromyces marxianus during high-temperature ethanol fermentation

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Stress Biology 2023年第1期3卷 288-299页

作者： Yumeng li Shiqi Hou Ziwei Ren Shaojie Fu Sunhaoyu Wang Mingpeng Chen Yan Dang Hongshen li Shizhong li pengsong li Beijing Key Lab for Source Control Technology of Water Pollution College of Environmental Science and EngineeringBeijing Forestry UniversityBeijing 100083China Engineering Research Center for Water Pollution Source Control&Eco-Remediation College of Environmental Science and EngineeringBeijing Forestry UniversityBeijing 100083China Institute of New Energy Technology Tsinghua UniversityBeijing 100084China

The thermotolerant yeast Kluyveromyces marxianus is known for its potential in high-temperature ethanol fermentation,yet it suffers from excess acetic acid production at elevated temperatures,which hinders ethanol production.To better understand how the yeast responds to acetic acid stress during high-temperature ethanol fermentation,this study investigated its transcriptomic changes under this condition.RNA sequencing(RNA-seq)was used to identify differentially expressed genes(DEGs)and enriched gene ontology(GO)terms and pathways under acetic acid stress.The results showed that 611 genes were differentially expressed,and GO and pathway enrichment analysis revealed that acetic acid stress promoted protein catabolism but repressed protein synthesis during high-temperature fermentation.Protein-protein interaction(PPI)networks were also constructed based on the interactions between proteins coded by the DEGs.Hub genes and key modules in the PPI networks were identified,providing insight into the mechanisms of this yeast’s response to acetic acid stress.The findings suggest that the decrease in ethanol production is caused by the imbalance between protein catabolism and protein synthesis.Overall,this study provides valuable insights into the mechanisms of K.marxianus’s response to acetic acid stress and highlights the importance of maintaining a proper balance between protein catabolism and protein synthesis for high-temperature ethanol fermentation.

关键词： Kluyveromyces marxianus Acetic acid Transcriptomics Protein-protein interaction network

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Sm and S Co-doping to Construct Homo-hetero Cu Catalysts for Synergistic Enhancing CO_(2) Electroreduction

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Chinese Journal of Chemistry 2023年第12期41卷 1443-1449页

作者： Jiyuan liu pengsong li Jiahui Bi Yong Wang Qinggong Zhu Xiaofu Sun Jianling Zhang Zhimin liu Buxing Han Beijing National Laboratory for Molecular Sciences CAS Laboratory of Colloid and Interface and Thermodynamics CAS Research/Education Center for Excellence in Molecular Sciences Institute of Chemistry Chinese Academy of Sciences Beijing 100190 China School of Chemical Science University of Chinese Academy of Sciences Beijing 100049 China Shanghai Key Laboratory of Green Chemistry and Chemical Processes School of Chemistry and Molecular Engineering East China Normal University Shanghai 200062 China

Copper(Cu)is recognized as one of the most efficient metal catalysts that can perform the electrocatalytic CO_(2) reduction reaction(CO_(2)RR)and its surface oxidation state determines the reaction pathway.The Cuδ+(0livered a high HCOOH Faradaic efficiency(FE)of 92.1%at the current density of 300 mA·cm^(–2) using 1 mol/L KOH aqueous solution as electrolyte,and the reduction potential was as low as–0.52 V vs.reversible hydrogen electrode(RHE).The co-doping of Sm and S resulted in excellent CO_(2)RR performance owing to the synergistic effect of the homo-hetero structure.The homo-doping of S could effectively adjust the electronic structure of Cu in favor of the formation of abundant Cu^(δ+)species.The existence of hetero-Sm species could not only stabilize the Cu^(δ+)sites,but also increase the concentration of H ions to form a favorable catalytic environment for HCOOH generation.

关键词： Carbon dioxide Electrochemistry Lanthanides Homo-hetero doping Copper

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Topotactic reduction of layered double hydroxides for atomically thick two-dimensional non-noble-metal alloy

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Nano Research 2017年第9期10卷 2988-2997页

作者： pengsong li Qixian Xie lirong Zheng Guang Feng Yingjie li Zhao Cai Yongmin Bi Yaping li Yun Kuang Xiaoming Sun Xue Duan State Key Laboratory of Chemical Resource Engineering Beijing University of Chemical Technology Beijing 100029 China College of Energy Beijing University of Chemical Technology Beijing 100029 China Beijing Advanced Innovation Centre for Soft Matter Science and Engineering Beijing University of Chemical Technology Beijing 100029 China Institute of High Energy Physics Chinese Academy of Sciences Beijing 100049 China

Layered double hydroxides (LDHs) have been widely used as catalysts owing to their tunable structure and atomic dispersion of high-valence metal ions; however, limited tunability of electronic structure and valence states have hindered further improvement in their catalytic performance. Herein, we reduced ultrathin LDH precursors in situ and topotactically converted them to atomically thick (~2 nm) two-dimensional (2D) multi-metallic, single crystalline alloy nanosheets with highly tunable metallic compositions. The as-obtained alloy nanosheets not only maintained the vertically aligned ultrathin 2D structure, but also inherited the atomic dispersion of the minor metallic compositions of the LDH precursors, even though the atomic percentage was higher than 20%, which is far beyond the reported percentages for single-atom dispersions (usually less than 0.1%). Besides, surface engineering of the alloy nanosheets can finely tune the surface electronic structure for catalytic applications. Such in situ topotactic conversion strategy has introduced a novel approach for atomically dispersed alloy nanostructures and reinforced the synthetic methodology for ultrathin 2D metal-based catalysts.

关键词： atomic thickness two-dimensional (2D) nanosheets non-noble-metal alloy atomic dispersion catalysis

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Layered double hydroxides with atomic-scale defects for superior electrocatalysis

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Nano Research 2018年第9期11卷 4524-4534页

作者： Oixian Xie Zhao Cai pengsong li Daojin Zhou Yongmin Bi Xuya Xiong Enyuan Hu Yaping li Yun Kuang Xiaoming Sun State Key Laboratory of Chemical Resource Engineering Beijing University of Chemical Technology Beijing 100029 China Department of Chemistry and Energy Sciences Institute Yale University West Haven Connecticut 06516 USA Chemistry Division Brookhaven National Laboratory Upzon New York 11973 USA College of Energy Beijing Advanced Innovation Center for Soft Matter Science and Engineering Beijing University of Chemical Technology Beijing 100029 China

Atomic composition tuning and defect engineering are effective strategies to enhance the catalytic performance of multicomponent catalysts by improving the synergetic effect; however, it remains challenging to dramatically tune the active sites on mulficomponent materials through simultaneous defect engineering at the atomic scale because of the similarities of the local environment. Herein, using the oxygen evolution reaction （OER） as a probe reaction, we deliberately introduced base-soluble Zn（II） or AI（III） sites into NiFe layered double hydroxides （LDHs）, which are one of the best OER catalysts. Then, the Zn（II） or AI（III） sites were selectively etched to create atomic M（I0/M（IIo defects, which dramatically enhanced the OER activity. At a current density of 20 mA.cm-2, only 200 mV overpotential was required to generate M（II） defect-rich NiFe LDHs, which is the best NiFe-based OER catalyst reported to date. Density functional theory （DFT） calculations revealed that the creation of dangling Ni-Fe sites （i.e., unsaturated coordinated Ni-Fe sites） by defect engineering of a Ni-O-Fe site at the atomic scale efficiently lowers the Gibbs free energy of the oxygen evolution process. This defect engineering strategy provides new insights into catalysts at the atomic scale and should be beneficial for the design of a variety of catalysts.

关键词： selective defect engineering atomic vacancy layered double hydroxide electrocatalysis oxygen evolution reaction

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Development of a whole-cell biosensor based on an ArsR-Pars regulatory circuit from Geobacter sulfurreducens

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Environmental Science and Ecotechnology 2021年第2期6卷 3-10页

作者： pengsong li Yumingzi Wang Xin Yuan Xinying liu Chunmao liu Xiaofen Fu Dezhi Sun Yan Dang Dawn E.Holmes Beijing Key Laboratory for Source Control Technology of Water Pollution Engineering Research Center for Water Pollution Source Control and EcoremediationCollege of Environmental Science and EngineeringBeijing Forestry UniversityBeijing 100083China Key Laboratory of Beijing Yanjing Beer Brewing Technology Beijing Yan Jing Brewery Company Ltd.Beijing 101300China MOST-USDA Joint Research Center for Biofuels Beijing Engineering Research Center for BiofuelsInstitute of New Energy TechnologyTsinghua UniversityBeijing 100084China Department of Physical and Biological Sciences Western New England University1215 Wilbraham RdSpringfieldMA 01119USA

In this study,an Escherichia coli(E.coli)whole-cell biosensor for the specific detection of bioavailable arsenic was developed by placing a green fluorescent protein(GFP)reporter gene under the control of the ArsR1(GSU2952)regulatory circuit from Geobacter sulfurreducens.E.coli cells only emitted green fluorescence in the presence of arsenite and were more sensitive to arsenite when they were grown in M9 supplemented medium compared to LB medium.Under optimal test conditions,the Geobacter arsR1 promoter had a detection limit of 0.01 mM arsenite and the GFP expression was linear within a range of 0.03-0.1 mM(2.25-7.5 mg/l).These values were well below World Health Organization’s drinking water quality standard,which is 10 mg/l.The feasibility of using this whole-cell biosensor to detect arsenic in water samples,such as arsenic polluted tap water and landfill leachate was verified.The biosensor was determined to be just as sensitive as atomic fluorescence spectrometry.This study examines the potential applications of biosensors constructed with Geobacter ArsR-Pars regulatory circuits and provides a rapid and cost-effective tool that can be used for arsenic detection in water samples.

关键词： Arsenic detection Whole-cell biosensor Geobacter sulfurreducens ars operon

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基于固有模态能量熵的多端混合直流线路方向暂态保护研究

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电网技术 2023年第11期47卷 4737-4745页

作者：邢超李朋松陈仕龙毕贵红邓健张梓航云南电网有限责任公司电力科学研究院云南省昆明市650217 昆明理工大学电力工程学院云南省昆明市650500

特高压多端混合直流输电线路首端整流侧边界、线路末端逆变侧边界不对称,T区边界不明显,线路保护故障区间的准确判别是目前亟需解决的重要问题。该文以昆柳龙特高压三端混合直流输电系统为研究对象,将保护元件安装于T区左右两侧,提出一... 详细信息

特高压多端混合直流输电线路首端整流侧边界、线路末端逆变侧边界不对称,T区边界不明显,线路保护故障区间的准确判别是目前亟需解决的重要问题。该文以昆柳龙特高压三端混合直流输电系统为研究对象,将保护元件安装于T区左右两侧,提出一种基于固有模态能量熵的多端混合直流线路方向暂态保护方法。论文分析特高压多端混合直流输电线路首端整流侧边界、线路末端逆变侧边界频率特性。根据T区两侧保护元件检测到的故障电流暂态能量的正负特性,提出T区左右两侧故障方向判据。利用互补集合经验模态分解(complementaryensembleempiricalmode decomposition,CEEMD)对故障暂态电流进行不同尺度分解,根据固有模态能量熵提出线路首端整流侧及线路末端逆变侧区内、外故障判据,解决了提取单一信号频带检测故障的局限性。分析和仿真结果表明,该保护方案仅利用T区左右两侧的保护元件就能实现特高压多端混合直流输电线路全线速动保护,且具有较好的耐受过渡电阻能力。

关键词：多端混合直流 T区边界频率特性固有模态能量熵方向暂态保护

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老龄化背景下广场舞锻炼与老年人死亡焦虑的关系:链式中介效应分析

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沈阳体育学院学报 2022年第2期41卷 63-69,128页

作者：刘雯静张钰潘妮李芃松大连理工大学体育与健康学院辽宁大连116024

为探讨广场舞锻炼与老年人死亡焦虑的关系及其作用机制,采用《死亡焦虑量表》《社会支持量表》《自我效能感量表》对385名60岁以上的老年人进行调查。结果表明:(1)广场舞锻炼、社会支持、自我效能感和死亡焦虑彼此间显著相关。(2)社会... 详细信息

为探讨广场舞锻炼与老年人死亡焦虑的关系及其作用机制,采用《死亡焦虑量表》《社会支持量表》《自我效能感量表》对385名60岁以上的老年人进行调查。结果表明:(1)广场舞锻炼、社会支持、自我效能感和死亡焦虑彼此间显著相关。(2)社会支持在广场舞锻炼和死亡焦虑间起单独中介作用,占总效应的38.84%;社会支持和自我效能感在广场舞锻炼和死亡焦虑间起链式中介效应,占总效应的15.03%。结论:广场舞锻炼通过社会支持和自我效能感的链式中介效应间接影响老年人的死亡焦虑水平;在老龄化背景下,广场舞锻炼对老年人的心理健康有积极的促进作用。

关键词：广场舞锻炼老年人死亡焦虑社会支持自我效能感

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植物油基紫外光固化材料研究进展

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华南农业大学学报 2022年第1期43卷 1-12页

作者：杨卓鸿李朋松楚状状袁腾胡洋生物基材料与能源教育部重点实验室/农业农村部现代农业材料重点实验室/华南农业大学材料与能源学院广东广州510642

全球性化石资源的过度消耗及环境污染问题的日益加剧,严重制约了石油基合成高分子材料产业的发展。以可再生植物油为原料,结合绿色高效的紫外光固化技术,构建植物油基紫外光固化材料“双绿色”应用体系,可望有效替代石油基合成高分子材... 详细信息

全球性化石资源的过度消耗及环境污染问题的日益加剧,严重制约了石油基合成高分子材料产业的发展。以可再生植物油为原料,结合绿色高效的紫外光固化技术,构建植物油基紫外光固化材料“双绿色”应用体系,可望有效替代石油基合成高分子材料,推动植物油在光固化领域的高值化发展。本文综述了近年来植物油基紫外光固化材料的研究进展,介绍了植物油结构与紫外光固化技术之间的联系,重点论述了大豆油、桐油、蓖麻油和亚麻油等典型植物油基紫外光固化材料的研究现状,分析了影响植物油基紫外光固化材料进一步发展的制约因素,并提出了该类材料未来的应用前景。

关键词：植物油紫外光固化技术大豆油桐油蓖麻油亚麻油

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智慧社区公共体育服务政策协同供给的府际关系研究

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沈阳体育学院学报 2023年第2期42卷 70-78页

作者：朱焱袁诗怡尚婉婷李芃松大连理工大学体育与健康学院辽宁大连116024 华东师范大学体育与健康学院上海200241

以104份政策文本为实证依据,运用社会网络分析法对我国智慧社区公共体育服务政策协同供给的府际关系进行研究,旨在为加快智慧社区建设与推动全民健身智慧化转型发展提供参考。结果表明:1)目前我国智慧社区公共体育服务政策协同供给关系... 详细信息

以104份政策文本为实证依据,运用社会网络分析法对我国智慧社区公共体育服务政策协同供给的府际关系进行研究,旨在为加快智慧社区建设与推动全民健身智慧化转型发展提供参考。结果表明:1)目前我国智慧社区公共体育服务政策协同供给关系呈现以中央政府为核心,以住房和城乡建设部、财政部、体育总局、国家发展和改革委员会等部门为传导基站的“倒T型”府际关系网络,辐射带动同级和下级部门进行智慧化公共体育服务协同供给,但整体网络密度较低,部门间协同供给关系仍然有较大提升空间。2)从纵向关系看,网络密度逐级递减,平均度数由高到低依次为中央、市级、省级、区级,从中央到地方的政策贯穿性不强,呈现自上而下的松散型网络结构特征。3)从横向关系看,四级关系网络呈现明显的“核心-边缘”化结构特征且存在较大的层级差异,中央联系较为紧密,地方联系相对松散。据此本文提出促进我国智慧社区公共体育服务政策协同供给的建议。

关键词：智慧社区公共体育服务政策协同府际关系社会网络

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我国公共体育场馆运营的困境及改革路径探究

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辽宁体育科技 2022年第4期44卷 52-56页

作者：杨雨龙李芃松大连理工大学体育与健康学院辽宁大连116023

当前我国公共体育场馆运营面临着新的挑战,如何在新发展格局中找到公共体育场馆运营新路径和提高运营自生动力,是当前亟待解决的难题。运用文献资料法,对我国公共体育场馆运营中存在的问题和困境进行分析,并提出改革新路径:加强公共体... 详细信息

当前我国公共体育场馆运营面临着新的挑战,如何在新发展格局中找到公共体育场馆运营新路径和提高运营自生动力,是当前亟待解决的难题。运用文献资料法,对我国公共体育场馆运营中存在的问题和困境进行分析,并提出改革新路径:加强公共体育场馆实质运营质量,促进可持续的、包容性的需求侧服务增长驱动力,增进公共体育场馆智慧构建与服务共赢,拓宽思路,以人民的利益为导向,积极创造个性化、多元化、精准化的智慧运营模式。

关键词：公共体育场馆运营改革路径困境

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