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Natural Variation in the Promoter of GSE5 Contributes to Grain Size Diversity in Rice

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Molecular Plant 2017年第5期10卷 685-694页

作者： penggen duan Jinsong Xu Dali Zeng Baolan Zhang Mufan Geng Guozheng Zhang Ke Huang Luojiang Huang Ran Xu Song Ge Qian Qian Yunhai Li State Key Laboratory of Plant Cell and Chromosome Engineering CAS Centre for Excellence in Molecular Plant Biology Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 P. R. China State Key Laboratory of Rice Biology China National Rice Research Institute Hangzhou 310006 China State Key Laboratory of Systematic and Evolutionary Botany Institute of Botany Chinese Academy of Sciences Beijing 100093 P. R. China University of Chinese Academy of Sciences Beijing 100039 P. R. China These authors contributed equally to this article

The utilization of natural genetic variation greatly contributes to improvement of important agronomic traits in crops. Understanding the genetic basis for natural variation of grain size can help breeders develop high- yield rice varieties. In this study, we identify a previously unrecognized gene, named GSE5, in the qSW5/ GW5 locus controlling rice grain size by combining the genome-wide association study with functional analyses. GSE5 encodes a plasma membrane-associated protein with ｜Q domains, which interacts with the rice calmodulin protein, OsCaMl-1. We found that loss of GSE5 function caused wide and heavy grains, while overexpression of GSE5 resulted in narrow grains. We showed that GSE5 regulates grain size predominantly by influencing cell proliferation in spikelet hulls. Three major haplotypes of GSE5 （GSE5, GSE5DELl＋IN1, and GSESDEL~ in cultivated rice were identified based on the deletion/insertion type in its pro- moter region. We demonstrated that a 950-bp deletion （DELl） in indica varieties carrying the GSE5DELl＋IN1 haplotype and a 1212-bp deletion （DEL2） in japonica varieties carrying the GSE5DEL2 haplotype associated with decreased expression of GSE5, resulting in wide grains. Further analyses indicate that wild rice acces- sions contain all three haplotypes of GSE5, suggesting that the GSE5 haplotypes present in cultivated rice are likely to have originated from different wild rice accessions during rice domestication. Taken together, our results indicate that the previously unrecognized GSE5 gene in the qSW5/GW5 locus, which is widely utilized by rice breeders, controls grain size, and reveal that natural variation in the promoter region of GSE5 contributes to grain size diversity in rice.

关键词： rice natural variation GSE5 grain size cell proliferation

The GW2-WG1-OsbZIP47 pathway controls grain size and weight in rice

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Molecular Plant 2021年第8期14卷 1266-1280页

作者： Jianqin Hao Dekai Wang Yingbao Wu Ke Huang penggen duan Na Li Ran Xu Dali Zeng Guojun Dong Baolan Zhang Limin Zhang Dirk Inze Qian Qian Yunhai Li State Key Laboratory of Plant Cell and Chromosome Engineering CAS Centre for Excellence in Molecular Plant BiologyInstitute of Genetics and Developmental BiologyThe Innovative Academy of Seed DesignChinese Academy of SciencesBeijing 100101China College of Life Sciences and Medicine Zhejiang Sci-Tech UniversityHangzhou 310018China State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhou 310006China Ghent University Department of Plant Biotechnology and Bioinformatics9052 GhentBelgium VIB Center for Plant Systems Biology 9052 GhentBelgium University of Chinese Academy of Sciences Beijing 100039China

Regulation of seed size is a key strategy for improving crop yield and is also a basic biological ***,the molecular mechanisms by which plants determine their seed size remain ***,we report that the GW2-WG1-OsbZIP47 regulatory module controls grain width and weight in ***1,which encodes a glutaredoxin protein,promotes grain growth by increasing cell ***,WG1 interacts with the transcription factor OsbZIP47 and represses its transcriptional activity by associating with the transcriptional co-repressor ASP1,indicating that WG1 may act as an adaptor protein to recruit the transcriptional *** contrary,OsbZIP47 restricts grain growth by decreasing cell *** studies reveal that the E3 ubiquitin ligase GW2 ubiquitinates WG1 and targets it for *** analyses confirm that GW2,WG1,and OsbZIP47 function in a comm on pathway to control grain *** together,ourfindi ngs reveal a genetic and molecular framework for the control of grain size and weight by the GW2-WG1-OsbZIP47 regulatory module,providing new targets for improving seed size and weight in crops.

关键词： rice WG1 GW2 OsbZIP47 grain size and weight cell proliferation

Control of Grain Size and Weight by the OsMKKK10-OsM KK4-OsMAPK6 Signaling Pathway in Rice

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Molecular Plant 2018年第6期11卷 860-873页

作者： Ran Xu penggen duan Haiyue Yu Zhengkui Zhou Baolan Zhang Ruci Wang Jing Li Guozheng Zhang Shangshang Zhuang Jia Lyu Na Li Tuanyao Chai Zhixi Tian Shanguo Yao Yunhai Li State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China CAS Centre for Excellence in Molecular Plant Biology Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China State Key Laboratory of Plant Genomics National Centre for Plant Gene Research (Beijing) Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China University of Chinese Academy of Sciences Beijing 100039 China

Grain size is one of the key agronomic traits that determine grain yield in crops. However, the mechanisms underlying grain size control in crops remain elusive. Here we demonstrate that the OsMKKK10-OsMKK4- OsMAPK6 signaling pathway positively regulates grain size and weight in rice. In rice, loss of OsMKKKIO function results in small and light grains, short panicles, and semi-dwarf plants, while overexpression of constitutively active OsMKKK10 （CA-OsMKKK10） results in large and heavy grains, long panicles, and tall plants. OsMKKK10 interacts with and phosphorylates OsMKK4. We identified an OsMKK4 gain-of-func- tion mutant （large11-1D）that produces large and heavy grains. OsMKK4A227T encoded by the large11-1D allele has stronger kinase activity than OsMKK4. Plants overexpressing a constitutively active form of OsMKK4 （OsMKK4oDD） also produce large grains. Further biochemical and genetic analyses revealed that OsMKKK10, OsMKK4, and OsMAPK6 function in a common pathway to control grain size. Taken together, our study establishes an important genetic and molecular framework for OsMKKK10-OsMKK4- OsMAPK6 cascade-mediated control of grain size and weight in rice.

关键词： Oryza sativa grain size MAPK signaling cell proliferation

Control of grain size by GS1 in rice

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Control of grain size by GS1 in rice

水稻窄叶突变体zy17的遗传分析和候选基因鉴定

2016年全国植物生物学大会

作者： Jianqin Hao Dekai Wang Yingbao Wu Ran Xu penggen duan Baolan Zhang Yunhai Li Institute of Genetics and Developmental Biology Chinese Academy of Sciences

Grain size is one of the most important agronomic traits in crops. Several factors that regulate grain size have been identified in rice,but the genetic and molecular mechanisms of grain size control remain almost unknown. Here,we describe the GS1 gene that encodes an unknown protein. Elevated expression of GS1 significantly increases grain size and weight by increasing cell proliferation in spikelet hulls and accelerating the grain milk filling rate,while downregulation of GS1 decreases grain size and weight. To understand the molecular mechanism of GS1 in grain size control,we have identified GS1-interacting proteins(GIP). Biochemical analyses show that GIP1 interacts with GS1 in vitro and in vivo and modulates the stability of GS1. GS1 also physically interacts with the transcription factor GIP2 to regulate its transcription activity. Thus,our findings define a novel genetic and molecular mechanism by which GIP1-GS1-GIP2 function to control grain size and weight in rice,suggesting this pathway is a promising target for rice grain yield improvement.

关键词： rice GS1 gene grain size interacting proteins

来源： cnki会议评论

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遗传 2015年第6期37卷 582-589页

作者：李雪倩徐冉段朋根伍应保罗越华李云海海南大学农学院海南省热带生物资源可持续利用重点实验室海口570228 中国科学院遗传与发育生物学研究所植物细胞与染色体工程国家重点实验室北京100101

器官大小调控是一个基本的发育生物学过程,受细胞分裂和细胞扩展的影响。然而,植物器官大小调控的遗传和分子机理仍不清楚。为了进一步了解器官大小调控的分子机制,文章分离了一系列水稻叶子宽窄改变的突变体。其中,窄叶突变体zy17叶变... 详细信息

器官大小调控是一个基本的发育生物学过程,受细胞分裂和细胞扩展的影响。然而,植物器官大小调控的遗传和分子机理仍不清楚。为了进一步了解器官大小调控的分子机制,文章分离了一系列水稻叶子宽窄改变的突变体。其中,窄叶突变体zy17叶变窄,同时伴有植株矮化、穗子变小、枝梗数和穗粒数降低的表型。遗传分析表明该窄叶性状受1个隐性基因控制;细胞学分析表明该突变体叶子的细胞数目和维管束数目显著降低,表明ZY17影响了细胞分裂。基因组重测序进一步筛选出ZY17的3个候选基因:Os02g22390基因突变发生在内含子区,编码蛋白为逆转座蛋白;Os02g28280和Os02g29530基因突变都发生在外显子区,其中Os02g28280编码一个功能未知蛋白,该基因突变后,发生碱基置换,产生非同义突变;Os02g29530编码一个含糖基转移酶相关的PFAM结构域的蛋白,该基因突变后,出现两个碱基的缺失,从而导致其蛋白翻译提前终止。对候选基因的深入研究,将揭示水稻叶子大小调控的机制。

关键词：水稻(Oryza sativa L.) 窄叶突变体细胞分裂遗传基础

一个新的OsBRI1弱等位突变体的鉴定及其调控种子大小的功能研究

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植物学报 2020年第3期55卷 279-286页

作者：管柳蓉刘祖培徐冉段朋根张国政于海跃李静罗越华李云海海南大学热带作物学院海南省热带生物资源可持续利用重点实验室海口570228 中国科学院遗传与发育生物学研究所植物细胞与染色体工程国家重点实验室北京100101 中国科学院大学北京100039

水稻(Oryza sativa)籽粒大小是影响其产量的关键农艺性状,克隆并研究水稻籽粒大小相关基因对于提高水稻产量具有重要意义。为深入探究水稻籽粒大小的调控机制,通过EMS诱变品种宽叶粳(KYJ),分离了一系列水稻籽粒大小改变的突变体,其中sm... 详细信息

水稻(Oryza sativa)籽粒大小是影响其产量的关键农艺性状,克隆并研究水稻籽粒大小相关基因对于提高水稻产量具有重要意义。为深入探究水稻籽粒大小的调控机制,通过EMS诱变品种宽叶粳(KYJ),分离了一系列水稻籽粒大小改变的突变体,其中smg12表现为籽粒变小,株高变矮,一级枝梗数和二级枝梗数减少。遗传分析表明,该小粒突变体受隐性单基因控制。细胞学分析显示,该突变体颖壳纵向细胞长度显著变短,表明SMG12主要影响细胞扩展。利用Mutmap方法对候选基因进行克隆,筛选出SMG12的候选基因OsBRI1,该基因编码油菜素内酯受体激酶。OsBRI1外显子上的第2074个碱基发生了由C到T的置换,产生非同义突变,使得该位置编码的脯氨酸变为丝氨酸,从而影响OsBRI1的功能。综上,该研究鉴定了OsBRI1基因的1个新等位变异,揭示了油菜素内酯途径调控水稻籽粒大小的细胞和分子基础。

关键词：水稻籽粒大小细胞扩展 Mutmap OsBRI1

Research on the Angular Glint of Targets in the Near-Zone

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Wuhan University Journal of Natural Sciences 2007年第3期12卷 481-484页

作者： XIA Yingqing TIAN Huamei duan Zhiguo WU Ling XU penggen College of Physical Science and Technology Huazhong Normal University Wuhan 430079 Hubei China School of Electronic Information Wuhan University Wuhan 430072 Hubei China

This paper presents a method to analyze and calculate the angular glint of targets. By parting the target to very small size cells, using high-frequency approximation, the near-field radar cross section （RCS） is calculated based on the scattering from complex targets and environments （SCTE） system, and the angular glint is calculated by the phase grads. The results show that the angular glint can be calculated exactly in the SCTE system, and this method is correct and efficient. In the near-zone, the far-field theory is not applicable and the angular glint should be calculated by the near-field theory.

关键词： RCS angular glint near-zone

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汽车盘毂精密锻造工艺仿真研究

锻压装备与制造技术 2016年第2期51卷 98-100页

作者：王石磊段兴旺杨祖强刘志奇武子威刘鹏根沧州嘉晟液压气动设备有限公司河北东光061600 太原科技大学山西太原030024

汽车盘毂是汽车离合器总成的重要组成零件,要求具有高强、高韧、耐冲击、抗疲劳等综合力学性能。传统的生产工艺由自由锻制坯和机械加工生产,产品力学性能及材料利用率较低,且生产中煤炭炉加热能耗巨大,污染严重。本公司与太原科技大学... 详细信息

汽车盘毂是汽车离合器总成的重要组成零件,要求具有高强、高韧、耐冲击、抗疲劳等综合力学性能。传统的生产工艺由自由锻制坯和机械加工生产,产品力学性能及材料利用率较低,且生产中煤炭炉加热能耗巨大,污染严重。本公司与太原科技大学机械工程学院进行产学研合作,提出汽车盘毂零件的精密闭式模锻成形工艺,并对成形工艺进行计算机仿真计算,通过理论分析和模拟,提出合理的锻造工艺;分析了零件精密闭式模锻变形过程的载荷变化规律、温度变化规律和摩擦系数对零件成形的影响,进一步修正了零件精密闭式模锻工艺。

关键词：精密锻造汽车盘毂 Deform 闭式模锻工艺