检索结果-南通市图书馆

Genetic variation and population dispersal of Yangtze voles Microtus fortis calamorum in the Dongting Lake region

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Current Zoology 2012年第2期58卷 211-220页

作者： Zongming GUO pengfei song Cong GUO Zhaobin song Yong WANG Bo LI Meiwen ZHANG Jianghong RAN College of Life Sciences Sichuan University Chengdu 610064 Sichuan China Key laboratory agro-ecological processes of Subtropical region Institute of Subtropical Agriculture Chinese Academy of Sciences Changsha 410125 China Yantai Nanshan University Yantai 265713 Shandong China

To understand genetic variation and population dispersal in the Yangtze vole Microtusfortis calamorum distributed in the Dongting Lake region, 144 individuals were collected from six habitat patches. The mitochondrial DNA control region was sequenced and 17 haplotypes were observed. Of the six investigated populations, haplotype and nucleotide diversities of those from larger patches were higher than those from smaller patches. Nonparametric correlation analysis showed that patch size had a positive correlation with haplotype diversity （r = 0.943, P 〈 0.01）. A neighbour-joining tree of the 17 haplotypes showed no geo- graphic genetic structure among the six populations. Analysis of isolation by distance showed that genetic differentiation among the six populations was not positively related to geographic distance. Analysis of mismatch distribution indicated that the voles had passed through a population expansion. The pattern of haplotype distribution in the Changsha population suggests that the population was established by a founder effect

关键词： Microtusfortis calamorum mitochondrial DNA control region genetic structure Dongting Lake Habitat patch

Effects of ex situ conservation on diversity and function of the gut microbiota of the Tibetan wild ass(Equus kiang)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Integrative Zoology 2023年第6期18卷 1089-1104页

作者： Hongmei GAO Feng JIANG Jingjie ZHANG Xiangwen CHI pengfei song Bin LI Zhenyuan CAI Tongzuo ZHANG Key Laboratory of Adaptation and Evolution of Plateau Biota Northwest Institute of Plateau BiologyChinese Academy of SciencesXiningChina Qinghai Provincial Key Laboratory of Animal Ecological Genomics XiningChina College of Life Sciences University of Chinese Academy of SciencesBeijingChina Department of Student Affairs Qinghai UniversityXiningChina

Ex situ conservation is the main method for the protection of endangered *** explore the effect of ex situ conservation on the gut microbiota of the kiang(Equus kiang),metagenomic sequencing combined with bioinformatics analysis was used to investigate the composition and function of the gut microbiota of the *** results showed that ex situ conservation not only protected wildlife,but also affected the composition and function of gut microbiota,as well as the health of *** the zoo,the ratio of the relative abundance of Firmicutes to that of Bacteroidetes(F/B)is higher,clusters of potentially pathogenic bacteria(such as Catonella,Catonella,and Mycoplasma)are more numerous,the abundance of resistance genes is higher,and the abundance of metabolic functions is *** dynamic changes of the gut microbiota also played an important role in the nutritional absorption,energy metabolism,and environmental adaptation of the *** the rearing environment and increasing food diversity play important roles for increasing the diversity of gut microbiota,reducing the spread of potentially pathogenic bacteria,and reducing *** the wild,especially in winter and in food-deficient areas,food supplementation can enhance the gut microbial homeostasis of wild animals and reduce the impact of *** depth studies of the gut microbial function of wildlife have important implications for improving ex situ conservation.

关键词： ex situ conservation metagenome potential pathogenic bacteria resistance genes Tibetan wild ass

Tuina alleviates neuropathic pain through regulate the activation of microglia and the secretion of inflammatory cytokine in spinal cord

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Traditional Chinese Medicine 2024年第4期44卷 762-769页

作者： WU Zhiwei ZHU Qingguang KONG Lingjun song pengfei ZHOU Xin GUO Guangxin ZHANG Shuaipan HE Tianxiang CHENG Yanbin FANG Min Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Shanghai University of Traditional Chinese MedicineShanghai 200437China Institute of Tuina Shanghai Institute of Traditional Chinese MedicineShanghai 200437China Shuguang Hospital Shanghai University of Traditional Chinese MedicineShanghai 200021China Department of Acupuncture and Tuina Shanghai University of Traditional Chinese MedicineShanghai 201203China

OBJECTIVE:To observe the analgesic effects of Tuina on neuropathic pain(NPP)and the underlying ***:Forty-eight Sprague-Dawley(SD)rats were assigned by random into three treatment groups:sham,chronic constriction injury(CCI),and *** group contained sixteen *** model was generated by ligating the right sciatic *** changes of CCI were assessed by the paw withdrawal threshold(PWT)and paw withdrawal latency(PWL).In addition,biochemical techniques such as immunofluorescence staining,enzyme-linked immunosorbent assay(ELISA)and Western blotting were used to profile levels of microglia activation and inflammatory factors in the spinal dorsal horn(SDH)of ***(clockwise pressing and rubbing)was performed at Chengshan(BL57)to observe the analgesic effects on CCI rats and the underlying ***:Rats with CCI experienced significant reduction in the PWT and PWL of the right hind paw relative to CCI group at day *** treatment rescued this situation significantly on days 10 and ***,Iba-1,microglia M1 receptor CD68,tumor necrosis factor-α(TNF-α),and interleukin-1β(IL-1β)were higher in the right SDH for CCI group compared to the sham group on day *** expected,Tuina partially downregulated the CCI-induced overexpressed Iba-1,CD68,TNF-α,and IL-1βin the SDH of CCI ***:Tuina induces a time-dependent cumulative analgesic effect in CCI rats by inhibiting the activation of microglia and the secretion of IL-1βand TNF-αin SDH.

关键词： Tuina neuralgia microglia inflammatory cytokine spinal cord

A paper-based microfluidic platform with shapememory-polymer-actuated fluid valves for automated multi-step immunoassays

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Microsystems & Nanoengineering 2019年第1期5卷 238-249页

作者： Hao Fu pengfei song Qiyang Wu Chen Zhao Peng Pan Xiao Li Nicole YKLi-Jessen Xinyu Liu Department of Mechanical and Industrial Engineering University of TorontoTorontoONM5S 3G8Canada Department of Mechanical Engineering McGill UniversityMontrealQCH3A 0C3Canada Department of Electrical and Electronic Engineering Xi’an Jiaotong-Liverpool University215123 SuzhouJiangsuChina School of Communication Sciences and Disorders McGill UniversityMontrealQC H3A 1G1Canada Institute of Biomaterials and Biomedical Engineering University of TorontoTorontoON M5S 3G9Canada Present address:Department of Chemistry Stanford UniversityStanfordCA 94305USA

Smart fluid manipulation with automatically controlled paper valves will enable automated and multi-step immunoassays on paper-based microfluidic *** this work,we present an integrated paper-based microfluidic platform with shape-memory polymer(SMP)-actuated fluid valves capable of automated colorimetric enzyme-linked immunosorbent assays(ELISAs).A single-layer microfluidic paper-based analytical device(μPAD)was designed to store all the reagents on the chip,and sequentially transfer reagents to a paper test zone following a specific ELISA protocol through automatic fluidic flow control by the multiple SMP-actuated *** actuation of a paper valve was based on the thermally responsive,duel-state shape transformation of a SMP sheet attached to the root of a paper cantilever beam for driving a hydrophilic paper bridge to connect and disconnect two paper channels.A portable colorimetric reader was developed to control the on-chip valve operations,quantify the colorimetric signal output,display the assay result,and wirelessly transmit the data to a smart phone for the application of *** operations of the paper valve and the entireμPAD were demonstrated with success rates of 97%and 93%,respectively.A detection mechanism for valve malfunction was designed and confirmed effective to identify any mal-operation of individual valves,thus rendering our platform reliable in real *** device calibration,we conducted direct ELISAs of rabbit IgG in phosphate-buffered saline(PBS),and achieved a low limit of detection(LOD)of 27 pM(comparable to that of standard and paper-based ELISAs).In order to demonstrate the clinical application of our multi-step immunoassay platform,we also conducted sandwich ELISAs to quantify the protein level of an inflammatory cytokine,namely tumor necrosis factor(TNF)-α,in surgically injured laryngeal tissues of *** protein levels of TNF-αwere shown similar between the conventional andμPAD ELISAs.

关键词： rabbit automated operations

Three-Dimensional Shear Wave Elastography Using a 2D Row Column Addressing(RCA)Array

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Biomedical Engineering Frontiers 2022年第1期3卷 337-347页

作者： Zhijie Dong Jihun Kim Chengwu Huang Matthew RLowerison U-Wai Lok Shigao Chen pengfei song Beckman Institute for Advanced Science and Technology University of Illinois Urbana-ChampaignUrbanaILUSA Department of Electrical and Computer Engineering University of Illinois Urbana-ChampaignUrbanaILUSA Division of ICT Convergence Engineering/Major in Electronic Engineering Kangnam UniversityRepublic of Korea Department of Radiology Mayo Clinic College of Medicine and ScienceRochesterMNUSA

*** develop a 3D shear wave elastography(SWE)technique using a 2D row column addressing(RCA)array,with either external vibration or acoustic radiation force(ARF)as the shear wave *** *** proposed method paves the way for clinical translation of 3D SWE based on the 2D RCA,providing a low-cost and high volume rate solution that is compatible with existing clinical *** is an established ultrasound imaging modality that provides a direct and quantitative assessment of tissue stiffness,which is significant for a wide range of clinical applications including cancer and liver *** requires high frame rate imaging for robust shear wave *** to the technical challenges associated with high volume rate imaging in 3D,current SWE techniques are typically confined to *** SWE from 2D to 3D is significant because of the heterogeneous nature of tissue,which demands 3D imaging for accurate and comprehensive ***.A 3D SWE method using a RCA array was developed with a volume rate up to 2000 *** performance of the proposed method was systematically evaluated on tissue-mimicking elasticity phantoms and in an in vivo case ***.3D shear wave motion induced by either external vibration or ARF was successfully detected with the proposed *** 3D shear wave speed maps were reconstructed for phantoms and in *** high volume rate 3D imaging provided by the 2D RCA array provides a robust and practical solution for 3D SWE with a clear pathway for future clinical translation.

关键词： Shear elasticity typically

A spiral microfluidic device for rapid sorting,trapping,and long-term live imaging of Caenorhabditis elegans embryos

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Microsystems & Nanoengineering 2023年第1期9卷 203-213页

作者： Peng Pan Zhen Qin William Sun Yuxiao Zhou Shaojia Wang pengfei song Yong Wang Changhai Ru Xin Wang John Calarco Xinyu Liu Department of Mechanical and Industrial Engineering University of Toronto5 King’s College RoadTorontoOntarioM5S 3G8Canada Upper Canada College 200 Lonsdale RoadTorontoOntarioM4V 1W6Canada School of Advanced Technology Xi’an Jiaotong-Liverpool University111 Ren’ai RoadSuzhou215000China School of Electronic and Information Engineering Suzhou University of Science and TechnologySuzhou215009China Department of Mechanical and Aerospace Engineering Jilin UniversityChangchun130012China Department of Cell&Systems Biology University of Toronto25 Harbord StTorontoOntarioM5S 3G5Canada Institute of Biomedical Engineering University of Toronto164 College StreetTorontoOntarioM5S 3G9Canada

Caenorhabditis elegans embryos have been widely used to study cellular processes and developmental regulation at early ***,most existing microfluidic devices focus on the studies of larval or adult worms rather than *** accurately study the real-time dynamics of embryonic development under different conditions,many technical barriers must be overcome;these can include single-embryo sorting and immobilization,precise control of the experimental environment,and long-term live imaging of *** paper reports a spiral microfluidic device for effective sorting,trapping,and long-term live imaging of single *** embryos under precisely controlled experimental *** device successfully sorts embryos from a mixed population of *** at different developmental stages via Dean vortices generated inside a spiral microchannel and traps the sorted embryos at single-cell resolution through hydrodynamic traps on the sidewall of the spiral channel for long-term *** the well-controlled microenvironment inside the microfluidic device,the response of the trapped *** embryos to mechanical and chemical stimulation can be quantitatively *** experimental results show that a gentle hydrodynamic force would induce faster growth of embryos,and embryos developmentally arrested in the high-salinity solution could be rescued by the M9 *** microfluidic device provides new avenues for easy,rapid,high-content screening of *** embryos.

关键词： larval spiral trapping

Possible mechanism underlying analgesic effect of Tuina in rats may involve piezo mechanosensitive channels within dorsal root ganglia axon

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Traditional Chinese Medicine 2018年第6期38卷 834-841页

作者： song pengfei Lin Zhigang Zhu Qingguang Jiang Shichao Zhang Hao Cheng Yanbin Sun Wuquan Wu Zhiwei Zhou Xin Lv Zhizhen Fang Min Department of Tuina YueYang Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese MedicineShanghai 200437China Fujian University of Traditional Chinese Medicine Subsidiary Rehabilitation Hospital Fuzhou 350003China Shanghai University of Traditional Chinese Medicine Shanghai 201203China Research Institute of Tuina Shanghai Academy of Traditional Chinese MedicineShanghai 200437China

OBJECTIVE: To demonstrate the analgesic effect of Tuina mainly from mechanically sensitive ion channels in peripheral myelinated nerve ***: A total of 40 healthy and pathogen-free adult male Sprague-Dawley rats were used in the study [weight:(220.0 ± 1.4) g, Shanghai Slac Laboratory Animal Co., Ltd., Shanghai, China; license No. Shanghai ICP 05033115]. The rats were housed in cages with free access to water and food in a temperature-controlled room [(22 ± 1) ℃] and12-h/12-h light-dark cycle. Thirty-two rats were randomly divided into five groups: naive, sham, chronic compression of dorsal root ganglion(CCD), Tuina(7 d) and Tuina(21 d). CCD rat model was established via unilateral DRG compression by"L"liked steel bar. Chinese Tuina treatment was accepted once per day. Behavior monitoring of paw withdrawal threshold(PWT) and paw withdrawal latency(PWL) were tested. The expression of Piezo1 and Piezo2 in myelinated nerve fiber were analyzed by immunohistochemistry and ***: There was a high expression of Piezo2 and a low expression of Piezo1 in the naive and CCD groups. In contrast, the expression of Piezo2 was down regulated and Piezo1 was increased after a period of Tuina. There was significant difference(P ≤ 0.05) between the ***: Our findings suggest that Tuina therapy can increase the expression of Piezo2 and decrease the expression of Piezo1 in the test *** different changes in the expressions of Piezo1 and Piezo2 may play an important role in alleviating CCD-induced allodynia and hyperalgesia.

关键词： Tuina Ganglia spinal Hyperalgesia Mechansensitiveion channels

Microfluidic-Assisted Caenorhabditis elegans Sorting:Current Status and Future Prospects

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Cyborg and Bionic Systems 2023年第1期4卷 330-350页

作者： Hang Yuan Wenwen Yuan Sixuan Duan Keran Jiao Quan Zhang Eng Gee Lim Min Chen Chun Zhao Peng Pan Xinyu Liu pengfei song School of Advanced Technology Xi'an Jiaotong-Liverpool UniversitySuzhouChina Department of Electrical and Electronic Engineering University of LiverpoolLiverpoolUK Department of Chemistry Xi’an Jiaotong-Liverpool UniversitySuzhouChina Department of Mechanical&Industrial Engineering University of TorontoTorontoCanada

Caenorhabditis elegans(***)has been a popular model organism for several decades since its first discovery of the huge research potential for modeling human diseases and *** is an important means of providing stage-or age-synchronized worm populations for many worm-based ***,conventional manual techniques for *** sorting are tedious and inefficient,and commercial complex object parametric analyzer and sorter is too expensive and bulky for most ***,the development of lab-on-a-chip(microfluidics)technology has greatly facilitated *** studies where large numbers of synchronized worm populations are required and advances of new designs,mechanisms,and automation *** previous reviews have focused on the development of microfluidic devices but lacked the summaries and discussion of the biological research demands of ***,and are hard to read for worm *** aim to comprehensively review the up-to-date microfluidic-assisted *** sorting developments from several angles to suit different background researchers,i.e.,biologists and ***,we highlighted the microfluidic *** sorting devices'advantages and limitations compared to the conventional commercialized worm sorting ***,to benefit the engineers,we reviewed the current devices from the perspectives of active or passive sorting,sorting strategies,target populations,and sorting ***,to benefit the biologists,we reviewed the contributions of sorting to biological *** expect,by providing this comprehensive review,that each researcher from this multidisciplinary community can effectively find the needed information and,in turn,facilitate future research.

关键词： sorting fluid engineer

Design and Application of Vehicle 48V Battery Fixing Structure Based on Collision Condition

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Design and Application of Vehicle 48V Battery Fixing Structu...