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中国畜牧兽医学会兽医产科学分会第十六次学术研讨会

作者： JIA Yanni GONG Suhua LI Haijing LIU Haokun SHANG Chunmei LI Zuhui JIN Yaping lin pengfei

Objective:The study aimed to characterize the expression of long non-coding RNA STAT3-AS in goat endometrium and its regulatory mechanism on the establishment of receptive endometrium in ***:Adult cyclic female dairy goats were mated with fertile males of the same strain to induce natural pregnancy,and collected goat uterine tissue on days 5,15,and 18 of *** goat endometrial epithelial cells（gEECs） in vitro,using E,P and IFNT to simulate the hormonal environment during early embryo adhesion in goat ***-qPCR,Western Blot,CCK8 and Flow cytometry were used to detect the expression of STAT3-AS in goat endometrium,as well as its effects on gEECs cell proliferation,apoptosis,cycle,and JAK/STAT signaling ***:Compared with the 5 th day of pregnancy,the expression level of STAT3-AS was significantly increased on the 15 th day of pregnancy（P<0.05）,but there was no significant change on the 18 th day of ***,the expression of STAT3-AS mRNA in the gEECs was significantly stimulated by IFNT treatment（P<0.05）,but E and P seemed to have not a regulating effect on STAT3-AS *** with the negative group,the expression level of STATAS was significantly down regulated after siRNA treatment（P<0.05）,and the silencing of STATAS expression significantly reduced the classic ISGs such as ISG15 and OAS2 mRNA levels in ESCs（P<0.05）.Inhibiting the expression of STAT3-AS can also significantly inhibit the proliferation of gEECs,but there have no significant effect on cell apoptosis and cell *** addition,after inhibiting the expression of STAT3-AS,the expression of STAT1 and STAT3 was significantly reduced at the mRNA level（P<0.05）,and the expression levels of P-STAT3 and PSTAT1 were also significantly reduced at the protein level（P<0.05）.Conclusion:In summary,these results indicate that STAT3-AS is regulated by IFNT,and it may regulate the establishment of endometrial receptivity of goats by influencing the proliferati

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Force-driven moisture-mediated CsPbBr_(3)nanocrystallization from amorphous glass

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Science China Materials 2024年第10期67卷 3106-3114页

作者： Peng Gao Hang lin pengfei Wang Ming Liu Ju Xu Yao Cheng Yuansheng Wang State Key Laboratory of Structural Chemistry Key Laboratory of Optoelectronic Materials Chemistry and PhysicsKey Lab Design&Assembly Funct NanostructFujian Institute of Research on the Structure of MatterChinese Academy of SciencesFuzhou 350002China Fujian Science&Technology Innovation Laboratory for Optoelectronic Information of China Fuzhou 350108China Fujian Provincial Key Laboratory of Terahertz Functional Devices and Intelligent Sensing School of Mechanical Engineering and AutomationFuzhou UniversityFuzhou 350108China University of Chinese Academy of Sciences Beijing 100049China

While the“amorphous to crystalline”transformation process,which has significant potential for application,has been widely studied,the microscopic mechanism on the nanometer scale is not fully *** contrast to common heat-driven phase transformations,the present study demonstrated the force-driven moisture-mediated nanocrystallization of perovskite CsPbBr_(3)precipitated from a glass *** the present case,the breakage of the glass network under shearing force produces high-energy sites to absorb H2O molecules/clusters from ambient moisture,and the hydration process promotes the crystallization *** analysis combined with confocal laser scanning microscopy revealed that the distribution of CsPbBr_(3)nanocrystals almost reproduced that of the localized stress field and clearly reflected the crack propagation *** potential applications of perovskite glass in the optical sensing of force and moisture are also *** findings provide insight into crystal nucleation/growth in glass,as well as understanding the dynamics of crack propagation during the brittle fracture process.

关键词： luminescence glass ceramic crystallization optical materials optical sensing CsPbBr_(3) perovskite

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Expression pattern analysis of STAT1/3 in goat endometrium during the embryo implantation period

Expression pattern analysis of STAT1/3 in goat endometrium d...

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中国畜牧兽医学会兽医产科学分会第八届会员代表大会暨第十五次学术研讨会

作者： LIU Haokun WANG Caixia LI Zuhui SHANG Chunmei ZHANG Xinyan ZHANG Ruixue JIN Yaping lin pengfei College of Veterinary Medicine Northwest A&F University Key Laboratory of Animal Biotechnology Ministry of Agriculture and Rural AffairsNorthwest A&F University

Objective:This study aimed to characterize the gene expression network of IFNT-treated goat endometrial epithelial cells(gEECs) and analyze the expression pattern of STAT1 and STAT3 in the gEECs and early pregnant uterus based on RNA-seq ***:The gEECs were treated with or without 20 ng/mL IFNT for RNA-seq *** Guanzhong dairy goats were mated with fertile males of the same strain to induce natural *** was ascertained by recovering embryos and elongated conceptus from the uterus,*** day 5,15 and 18,the whole uteri of pregnant goats were collected immediately by midventral ***:Differential comparison showed that there were 442 up-regulated differentially expressed genes(DEGs) and 510 down-regulated *** bioinformatics analyses,we found that DEGs were significantly enriched in immune-related functions or *** the enriched pathways,JAK-STAT signaling pathway activates signal transmission from cell membrane to nucleus,affecting many aspects of mammalian immune *** expression pattern of STAT1,STAT2,STAT3,and IRF9 mRNA were the same as the RNA-seq *** the change of mRNA levels,phosphorylated-STAT1 expression was markedly increased with 6 h after treated by IFNT when compared to the control group in *** the contrary,the level of p-STAT3 in gEECs decreased at 6 h and then increased with the treatment time after treated by *** in vivo experiments further showed that the mRNA and protein phosphorylation levels of STAT1 and STAT3 in the uterus were up-regulated from pre-implantation(5 days of pregnancy) to embryo adhesion period(18 days of pregnancy).Additionally,the expression of STAT1 protein decreased in luminal epithelium(LE) and superficial glandular epithelium(sGE) during the peri-implantation period(15 days of pregnancy),but increased on 18 days of pregnancy;p-STAT1 was highly expressed in deep glandular epithelium(GE) on 18 days of ***3 was highly expresse

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Expression of Luman/CREB3 in testis and the biological significance in testosterone production

Expression of Luman/CREB3 in testis and the biological signi...

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中国畜牧兽医学会兽医产科学分会第十三次学术研讨会

作者： Lei Wang pengfei lin Fenglei Chen YongJie Xiong Xiao Li Nan Wang Aihua Wang YaPing Jin Key Laboratory of Animal Biotechnology of the Ministry of Agriculture Northwest A&F University College of Veterinary Medicine Northwest A&F University

Luman/CREB3 has been identified as a novel transcriptional co-regulator of a variety of nuclear *** report herein for the first time that Luman is expressed in male mouse *** and real-time PCR results showed positive Luman immunostaining in Leydig cells and the expression pattern of Luman in mouse testis exhibited an age-related *** staining of Luman in the MLTC-1 Leydig cell line indicates that Luman is located within the *** significantly up-regulated the levels of expression of Luman in MLTC-1 cells and mouse testes,*** elucidate the function of Luman,experiments were conducted to examine the consequences of lentiviral-mediated RNA interference(RNAi) of Luman on testosterone production and expression of several genes involved in *** silencing caused a increase in HCG-stimulated testosterone *** addition,Luman silencing significantly up-regulated the mRNA levels of P450 scc and P450c17 in primary Ledyig cells and MLTC-1 ***,our data are the first to provide evidence for a possible direct action of Luman in the control of testicular ***,these data indicate that the expression of Luman in mouse testis is stage dependent and androgen inductive,and suggest the important role of Luman in male reproduction.

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祁连山地区林下地被物持水量与采样方法的关系研究

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冰川冻土 2021年第2期43卷 610-617页

作者：杨军军何志斌蔺鹏飞咸阳师范学院陕西咸阳712000 中国科学院西北生态环境资源研究院中国生态系统研究网络临泽内陆河流域研究站/内陆河流域生态水文重点实验室甘肃兰州730000

林下地被物持水量的准确计算对生态环境脆弱的寒区水文功能的模拟和预测尤为关键。本研究以青藏高原北麓的祁连山典型流域为研究区,对原状和散状两种采样方法下,青海云杉林下苔枯层(苔藓与枯落物形成的层状结构)持水特征参数的差异进行... 详细信息

林下地被物持水量的准确计算对生态环境脆弱的寒区水文功能的模拟和预测尤为关键。本研究以青藏高原北麓的祁连山典型流域为研究区,对原状和散状两种采样方法下,青海云杉林下苔枯层(苔藓与枯落物形成的层状结构)持水特征参数的差异进行分析,探讨不同采样方法在林下苔枯层水文特征研究时的影响。本研究中原状样本采用PVC管取样,散状样本采用尼龙网袋取样,获得2 596个实验数据。研究发现平均厚度为14.3cm的原状苔藓与苔枯样本最大持水量分别为2.29kg·m^(-2)与5.42kg·m^(-2),最大持水率分别为2 071%与890%,散状苔藓最大持水率为610%。苔枯层与苔藓层的最大持水量差异显著,苔枯层相比苔藓层具有更好的保水功能,林下地被物水文特征量化时应区别对待;相比于原状样本,散状采样方法下苔藓层的最大持水率被低估近70.5%,即在散状采样方法下,林下苔藓层的最大持水率会被严重低估;地被物脱湿曲线为幂函数,蒸发时长为4.8~5.6h,关键参数的估算对于地被物水分蒸发过程的模拟和预测具有重要参考价值。不同采样方法对地被物持水量的估算差异显著,实践中应根据实验目的和研究对象,选择合适的采样方法。

关键词：寒区水文最大持水量地被物苔藓祁连山

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潮汐混合对大西洋经圈翻转环流(AMOC)模拟影响的数值模拟研究

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大气科学 2017年第5期41卷 1087-1100页

作者：于子棚刘海龙林鹏飞中国科学院大气物理研究所大气科学和地球流体力学数值模拟国家重点试验室北京100029 中国科学院大学北京100049

海洋中的潮汐混合对大西洋经圈翻转环流AMOC(Atlantic Meridional Overturning Circulation)模拟的影响是海洋环流模式研究的热点问题之一。本文采用IAP/LASG发展的气候系统海洋模式LICOM(LASG/IAP Climate system Ocean Model)及与海冰耦合模式进行了有无潮汐混合方案的试验,重点探讨了潮汐混合对AMOC强度模拟的影响。结果显示,引入潮汐混合后模拟的AMOC强度极大值比对照试验增加约1倍,更接近RAPID(Rapid Climate Change Programme)观测。而且,潮汐混合试验中模拟的AMOC上层环流深度(3200 m)比对照试验加深1000 m左右,同样更接近RAPID观测。海洋底部的垂直混合增强,使海洋层结变得更加不稳定,加强了北大西洋高纬地区,特别是拉布拉多海等地区的深对流,这是AMOC加强的直接原因。同时,潮汐混合试验中上层海洋环流也加强,增加了中低纬副热带高盐海水向高纬输送,使表层增密,海洋层结更加不稳定,也可以进一步增强AMOC。

关键词： LASG/IAP气候系统海洋模式潮汐混合大西洋经圈翻转环流

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热带太平洋盐度年际变化对海表温度异常作用比较:1997/1998、2014/2015和2015/2016年El Nino事件

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大气科学 2020年第5期44卷 1057-1075页

作者：石世玮智海林鹏飞陈涛南京信息工程大学气象灾害教育部重点实验室/气候与环境变化国际合作联合实验室/气象灾害预报预警与评估协同创新中心南京210044 中国科学院大气物理研究所大气科学和地球流体力学数值模拟国家重点实验室(LASG) 北京100029

海洋盐度变化为研究气候变化的机制提供了一个新的视角。本文通过对比1997/1998年、2015/2016年两次强厄尔尼诺(El Nino)事件和2014/2015年特殊El Nino事件,对盐度变化及其影响海表面温度异常(SSTA)的物理过程进行了比较分析。研究表明,... 详细信息

海洋盐度变化为研究气候变化的机制提供了一个新的视角。本文通过对比1997/1998年、2015/2016年两次强厄尔尼诺(El Nino)事件和2014/2015年特殊El Nino事件,对盐度变化及其影响海表面温度异常(SSTA)的物理过程进行了比较分析。研究表明,El Nino和南方涛动(El Nino–Southern Oscillation, ENSO)发展的强弱与热带西太平洋大范围海表层盐度异常(SSSA)及其向东扩散的差异有明显关联。1997/1998、2015/2016年赤道东太平洋SSTA的增暖,对应两次强El Nino事件,在发生年4月,中西太平洋海域出现了明显的负SSSA,之后东移至日期变更线以西,SSSA引发的混合层深度(MLD)变浅、障碍层厚度(BLT)变厚,导致热带中—西太平洋表层升温增强,促使了赤道中太平洋的早期变暖;2014/2015年弱El Nino事件虽然在发生年4月,位于赤道中西太平洋出现了负SSSA,但没有发展东移,导致BLT的增厚过程减弱,对表层温度的调制作用减弱甚至消失。三次事件对应的盐度变化过程中,水平平流和淡水通量(FWF)引起的表层强迫是影响盐度收支的主要因子,水平平流影响盐度异常的前期变化,触发事件的发生;热带太平洋西部降水引起的FWF负异常的影响最为显著,对ENSO异常信号出现后SSSA的维持起决定性作用。相比较两次强El Nino事件,2014/2015年El Nino对应的早期FWF负异常没有发展和东移,并且之后迅速减弱,导致中西太平洋盐度负趋势减缓,MLD加深,BLT变薄,促使上表层海水冷却,抑制了赤道东太平洋的早期变暖和ENSO发展。研究结果表明,盐度变化与ENSO密切相关,热带中西太平洋海域早期表层盐度变化可能可以作为SSTA的指数。特别地,SSSA在调节SSTA时,不仅影响它的强度,而且可以作为判断ENSO是否发展及其强弱的前兆因子。

关键词：盐度异常年际变化强厄尔尼诺热带太平洋

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Genome-wide analysis and functional prediction of long non-coding RNAs in mouse uterus during the implantation window

Genome-wide analysis and functional prediction of long non-c...

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中国畜牧兽医学会兽医产科学分会第十三次学术研讨会

作者： Qi Wang Nan Wang Rui Cai Fan Zhao Yongjie Xiong Xiao Li Aihua Wang pengfei lin Yaping Jin College of Veterinary Medicine Northwest A&F University Key Laboratory of Animal Biotechnology Ministry of AgricultureNorthwest A&F University

Successful implantation is dependent on the intricate genetic and molecular dialogue between the receptive uterus and active *** of the receptive uterus is a crucial step for embryo *** this study,the expression profiles and characterization of long non-coding RNAs(IncRNAs)in uterine samples from pregnant mice on day 4(D4U),day 5 at implantation sites(D5IU)and

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基于六维加速度传感器的大型机械臂柔性关节振动抑制

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空间控制技术与应用 2018年第5期44卷 7-13页

作者：倪风雷林鹏飞邹添哈尔滨工业大学机器人技术与系统国家重点实验室哈尔滨150080

空间大型机械臂具有关节柔性大、臂杆长、基座漂浮等特点,将导致其在自由空间运动存在较大的残余振动,约束空间力控制精度和稳定性较低.基于设计的机械臂末端六维加速度传感器,设计关节加速度反馈控制器,并对控制性能进行分析对比,最后... 详细信息

空间大型机械臂具有关节柔性大、臂杆长、基座漂浮等特点,将导致其在自由空间运动存在较大的残余振动,约束空间力控制精度和稳定性较低.基于设计的机械臂末端六维加速度传感器,设计关节加速度反馈控制器,并对控制性能进行分析对比,最后搭建机械臂柔性关节实验平台,开展关节空间位置和阻抗控制试验,试验结果表明基于加速度反馈的残余振动相比于采用传统的输入整形方法,具有振动抑制快,振幅小等优点.

关键词：六维加速度柔性关节振动抑制机械臂

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Knock-down of apoptosis inducing factor gene protects endoplasmic reticulum stress-mediated goat granulosa cell apoptosis

Knock-down of apoptosis inducing factor gene protects endopl...

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中国畜牧兽医学会兽医产科学分会第十三次学术研讨会

作者：杨迪琦 Tingting Jiang pengfei lin Huatao Chen Lei Wang Nan Wang Fan Zhao Aihua Wang 靳亚平 Key Laboratory of Animal Biotechnology of the Ministry of Agriculture Northwest A&F University College of Veterinary Medicine Northwest A&F University

The apoptosis of granulosa cells is the main cause of follicular atresia,and endoplasmic reticulum(ER)stress is involved in the apoptosis of granulosa *** inducing factor(AIF) mediates caspase-independent apoptosis and causes chromatin condensation and DNA fragmentation,but its role in ER stress-mediated granulosa cell apoptosis during goat follicular atresia remains largely *** aim of this study was to investigate the function of AIF in the apoptosis of goat granulosa cells media ted by ER *** results of immunohistochemical and Western blot analyses demonstrated that AIF was mainly located in granulosa cells,and the expression of AIF significantly increased during follicular ***,AIF-shRNA recombinant lentiviral vectors were constructed successfully and transfected into hTERT-goat granulosa cells(hTERT-GGCs).Real-time quantitative PCR and Western blot analysis confirmed that AIF was effectively knocked down in *** cytometry results showed that the knock-down of AIF in hTERT-GGCs reduced apoptosis due to serum starvation or thapsigargin(Tg)***,AIF depletion changed the expression of related molecular markers molecules of ER stress under Tg *** conclusion,AIF may serve as a key factor during follicular atresia and AIF depletion protects ER stress-mediated goat granulosa cell apoptosis.

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