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Isolation and Expression Patterns of Rice WRKY82 Transcription Factor Gene Responsive to Both Biotic and Abiotic Stresses

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Agricultural Sciences in China 2011年第6期10卷 893-901页

作者： peng xi-xu TANG xin-ke ZHOU Ping-lan HU Yao-jun DENG xiao-bo HE Yan WANG Hai-hua School of Life Sciences Hunan University of Science and Technology Xiangtan 411201 P.R.China

WRKY transcription factors are involved in the regulation of response to biotic and abiotic stresses in plants. A full-length cDNA clone of rice WRKY82 gene （OsWRKY82） was isolated from a cDNA library generated from leaves infected by Magnaporthe grisea. OsWRKY82 contained an entire open reading frame in length of 1 701 bp, and was predicted to encode a polypeptide of 566 amino acid residues consisting of two WRKY domains, each with a zinc finger motif of C2H2, belonging to the WRKY subgroup I. OsWRKY82 shared high identity at the amino acid level with those from Sorghum bicolor, Hordeum vulgare, and Zea mays. The transcript level of OsWRKY82 was relatively higher in stems, leaves, and flowers, and less abundant in grains. It was induced by inoculation with M. grisea and Rhizoctonia solani. However, the inducible expression in incompatible rice-M. grisea interactions was earlier and greater than that in compatible interactions. The expression of OsWRKY82 was up-regulated by methyl jasmonate and ethephon, whereas salicylic acid exerted no effects on its expression. Moreover, OsWRKY82 exhibited transcriptional activation ability in yeast. Additionally, OsWRKY82 transcripts could be induced by wounding and heat shocking, but not by abscisic acid, cold, high salinity and dehydration. By contrast, gibberellin suppressed the expression of OsWRKY82. These indicate that OsWRKY82 is a multiply stress-inducible gene responding to both biotic and abiotic stresses, and may be involved in the regulation of defense response to pathogens and tolerance against abiotic stresses by jasmonic acid/ethylene-dependent signaling pathway.

关键词： WRKY transcription factor biotic stress abiotic stress gene expression Oryza sativa

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Developing biolmaging and quantitative methods to study 3D genome

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Frontiers of Electrical and Electronic Engineering in China 2016年第2期4卷 129-147页

作者： Juntao Gao Xusan Yang Mohamed Nadhir Djekide Yang Wang peng xi Michael Q. Zhang MOE Key Laboratory of Bioinformatics BioinformatJcs Division and Center for Synthetic & Systems Biology TNLIST Department of Automation Tsinghua University Beijing 100084 China Department of Biomedical Engineering College of Engineering Peking University Beijing 100871 China De~)artment of Biological Sciences Center for SystemsBiology.the University of Texas at Dallas Richardson TX 75080-3021USA

The recent advances in chromosome configuration capture （3C）-based series molecular methods and optical super- resolution （SR） techniques offer powerful tools to investigate three dimensional （3D） genomic structure in prokaryotic and eukaryotic cell nucleus. In this review, we focus on the progress during the last decade in this exciting field. Here we at first introduce briefly genome organization at chromosome, domain and sub-domain level, respectively; then we provide a short introduction to various super-resolution microscopy techniques which can be employed to detect gcnome 3D structure. We also reviewed the progress of quantitative and visualization tools to evaluate and visualize chromatin interactions in 3D genome derived from Hi-C data. We end up with the discussion that imaging methods and 3C-based molecular methods are not mutually exclusive -- actually they arc complemental to each other and can be combined together to study 3D genome organization.

关键词： 3D Genome quantitative methods bioimaging super resolution

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基于锚点的无监督跨模态哈希算法

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软件学报 2024年第8期35卷 3739-3751页

作者：胡鹏彭玺彭德中四川大学计算机学院四川成都610065 成都瑞贝英特信息技术有限公司四川成都610094

基于图的无监督跨模态哈希学习具有存储空间小、检索效率高等优点,受到学术界和工业界的广泛关注,已成为跨模态检索不可或缺的工具之一.然而,图构造的高计算复杂度阻碍其应用于大规模多模态应用.主要尝试解决基于图的无监督跨模态哈希... 详细信息

基于图的无监督跨模态哈希学习具有存储空间小、检索效率高等优点,受到学术界和工业界的广泛关注,已成为跨模态检索不可或缺的工具之一.然而,图构造的高计算复杂度阻碍其应用于大规模多模态应用.主要尝试解决基于图的无监督跨模态哈希学习面临的两个重要挑战:1)在无监督跨模态哈希学习中如何高效地构建图?2)如何解决跨模态哈希学习中的离散值优化问题?针对这两个问题,分别提出基于锚点图的跨模态学习和可微分哈希层.具体地,首先从训练集中随机地选择若干图文对作为锚点集,利用该锚点集作为中介计算每批数据的图矩阵,以该图矩阵指导跨模态哈希学习,从而能极大地降低空间与时间开销;其次,提出的可微分哈希层可在网络前向传播时直接由二值编码计算,在反向传播时亦可产生梯度进行网络更新,而无需连续值松弛,从而具有更好的哈希编码效果;最后,引入跨模态排序损失,使得在训练过程中考虑排序结果,从而提升跨模态检索正确率.通过在3个通用数据集上与10种跨模态哈希算法进行对比,验证了提出算法的有效性.

关键词：无监督哈希学习跨模态检索锚点图可微分哈希公共汉明空间

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杉木叶片性状不支持广为接受的通量性状网络假说

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Journal of Plant Ecology 2023年第2期16卷 151-161页

作者： xi peng Meifang Zhao Shuguang Liu Wende Yan College of Life Science and Technology Central South University of Forestry and TechnologyChangsha 410004China Huitong National Field Station for Scientific Observation and Research of Chinese Fir Plantation Ecosystem in Hunan Province Huitong 410015China National Engineering Laboratory for Applied Technology of Forestry and Ecology in Southern China Changsha 410004China

“高叶脉密度-更快生长和更高的生产力”模型和假说认为高叶脉密度能促进更高的碳和水的耦合效率,暗示了个体的快速生长和高林分生产力,这些假说被研究者广泛关注和认同。然而,这些模型和假说并没有包括足够的裸子植物样本,特别是在亚... 详细信息

“高叶脉密度-更快生长和更高的生产力”模型和假说认为高叶脉密度能促进更高的碳和水的耦合效率,暗示了个体的快速生长和高林分生产力,这些假说被研究者广泛关注和认同。然而,这些模型和假说并没有包括足够的裸子植物样本,特别是在亚热带栽培的针叶树种。我们在此研究了叶脉密度和叶功能性状的值和比例关系,这些样本来自于以快速生长著称的杉木(Cunninghamia lanceolata)的主要分布区。我们还检索了一个经验数据集,该数据集包括杉木的光合作用、生物化学、解剖学和水力特性。杉木叶脉密度(范围为0.34–1.09 mm/mm)远低于全球报道的范围(1–25 mm/mm),而杉木以快速生长和高产在中国长期闻名。我们进一步证实,较高的脉络密度与较小的叶片负相关(r=-0.71,P<0.001),这与被子植物中发现的情况一致。然而,我们发现,与全球物种(正相关)相比,杉木的叶脉密度-叶厚相关性和叶片寿命可塑性呈现相反的趋势,这种关系可能暗示功能效率和生产力间的权衡。我们的结果将为普适的生长规律提供有效的补充评估,包括评价区域植物性状特征、植物物种的配置和性状效率对水力潜能的影响。

关键词：叶脉密度叶片大小叶片厚度性状可塑性种类变异

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Ultra-high spatio-temporal resolution imaging with parallel acquisition-readout structured illumination microscopy(PAR-SIM)

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Light(Science & Applications) 2024年第6期13卷 1140-1158页

作者： xinzhu Xu Wenyi Wang Liang Qiao Yunzhe Fu xichuan Ge Kun Zhao Karl Zhanghao Meiling Guan xin Chen Meiqi Li Dayong Jin peng xi Department of Biomedical Engineering College of Future TechnologyPeking UniversityBeijing100871China Wallace H.Coulter Dept.of Biomedical Engineering Georgia Institute of Technology and Emory UniversityAtlanta30332GAUSA National Biomedical Imaging Center Peking UniversityBeijing100871China Department of Biomedical Engineering College of EngineeringSouthern University of Science and TechnologyShenzhen518055GuangdongChina Airy Technologies Co. Ltd.Beijing100086China Eastern Institute for Advanced Study Eastern Institute of TechnologyNingboZhejiang315200China School of Life Science Peking UniversityBeijing100871China Institute for Biomedical Materials and Devices(IBMD) Faculty of ScienceUniversity of Technology SydneySydneyNSW2007Australia

Structured illumination microscopy(SIM)has emerged as a promising super-resolution fluorescence imaging technique,offering diverse configurations and computational strategies to mitigate phototoxicity during real-time imaging of biological *** efforts to enhance system frame rates have concentrated on processing algorithms,like rolling reconstruction or reduced frame reconstruction,or on investments in costly sCMOS cameras with accelerated row readout *** this article,we introduce an approach to elevate SIM frame rates and region of interest(ROI)coverage at the hardware level,without necessitating an upsurge in camera expenses or intricate ***,parallel acquisition-readout SIM(PAR-SIM)achieves the highest imaging speed for fluorescence imaging at currently available detector *** using the full frame-width of the detector through synchronizing the pattern generation and image exposure-readout process,we have achieved a fundamentally stupendous information spatial-temporal flux of 132.9 MPixels·s^(−1),9.6-fold that of the latest techniques,with the lowest SNR of−2.11 dB and 100 nm ***-SIM demonstrates its proficiency in successfully reconstructing diverse cellular organelles in dual excitations,even under conditions of low signal due to ultra-short exposure ***,mitochondrial dynamic tubulation and ongoing membrane fusion processes have been captured in live COS-7 cell,recorded with PAR-SIM at an impressive 408 *** posit that this novel parallel exposure-readout mode not only augments SIM pattern modulation for superior frame rates but also holds the potential to benefit other complex imaging systems with a strategic controlling approach.

关键词： hardware illumination resolution

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Recent developments in application of single-cell RNA sequencing in the tumour immune microenvironment and cancer therapy

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Military Medical Research 2023年第3期10卷 383-402页

作者： Pei-Heng Li xiang-Yu Kong Ya-Zhou He Yi Liu xi peng Zhi‑Hui Li Heng Xu Han Luo Jihwan Park Department of Thyroid and Parathyroid Surgery Laboratory of Thyroid and Parathyroid DiseaseFrontiers Science Centre for Disease‑Related Molecular NetworkDepartment of General SurgeryWest China HospitalSichuan UniversityChengdu 610044China Department of Oncology West China School of Public Health and West China Fourth HospitalSichuan UniversityChengdu 610044China Department of Rheumatology and Immunology Rare Diseases CentreWest China HospitalSichuan UniversityChengdu 610044China College of Computer Science Sichuan UniversityChengdu 610065China State Key Laboratory of Biotherapy and Cancer Centre West China HospitalSichuan University and Collaborative Innovation CentreChengdu 610044China School of Life Sciences Gwangju Instituteof Science and Technology(GIST)Gwangju 61005Republic of Korea

The advent of single-cell RNA sequencing(scRNA-seq)has provided insight into the tumour immune microenvironment(TIME).This review focuses on the application of scRNA-seq in investigation of the *** time,scRNA-seq methods have evolved,and components of the TIME have been deciphered with high *** this review,we first introduced the principle of scRNA-seq and compared different sequencing *** cell types in the TIME,a continuous transitional state,and mutual intercommunication among TIME components present potential targets for prognosis prediction and treatment in ***,we concluded novel cell clusters of cancerassociated fibroblasts(CAFs),T cells,tumour-associated macrophages(TAMs)and dendritic cells(DCs)discovered after the application of scRNA-seq in *** also proposed the development of TAMs and exhausted T cells,as well as the possible targets to interrupt the *** addition,the therapeutic interventions based on cellular interactions in TIME were also *** decades,quantification of the TIME components has been adopted in clinical practice to predict patient survival and response to therapy and is expected to play an important role in the precise treatment of *** the current findings,we believe that advances in technology and wide application of single-cell analysis can lead to the discovery of novel perspectives on cancer therapy,which can subsequently be implemented in the ***,we propose some future directions in the field of TIME studies that can be aided by scRNA-seq technology.

关键词： Single-cell RNA sequencing(scRNA-seq) Tumour immune microenvironment(TIME) Trajectory Cellular interactions Therapeutic targets

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The impact of damming on geochemical behavior of dissolved inorganic carbon in a karst river

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Chinese Science Bulletin 2014年第4期59卷 2348-2355页

作者： xi peng Cong-Qiang Liu Baoli Wang Yan-Chuang Zhao State Key Laboratory of Environmental Geochemistry Institute of Geochemistry Chinese Academy of Sciences College of Earth Science University of Chinese Academy of Sciences

To determine the impact of damming on the geochemical behavior of dissolved inorganic carbon(DIC)in the Wujiang River basin,field measurements and samples were collected semimonthly for a *** surface waters of the reservoirs contained concentrations of chlorophyll a up to 5.6 times higher than the upstream *** with the entering waters,the contributions of HCO3-and dissolved CO2to DIC decreased,and the contribution of CO32-to DIC and the carbon isotopic composition of DIC(d13CDIC)increased in the surface waters of the reservoirs,while in the waters released,the DIC species showed reverse geochemical *** d13CDICranged from-10.2%to 2.5%,indicating that significant contributions were from carbonate weathering,photosynthesis,and *** the damming of a river,the bioactivity of phytoplankton was enhanced,and this affected the geochemistry of DIC compared to an unimpacted river and d13CDICcan be used to discern these ***-frequency monitoring of river-reservoir systems is necessary to evaluate the efflux of CO2and provide a better understanding of the carbon sinks and sources in the impounded river.

关键词：地球化学行为溶解无机碳河流筑坝喀斯特碳同位素组成水库系统 DIC

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±500kV天广直流输电系统交流滤波器频繁投切分析

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电网技术 2005年第4期29卷 1-3,22页

作者：蔡希鹏中国南方电网超高压输电公司广东省广州市510620

天(生桥)—广(州)±500kV直流输电工程已于2001年6月双极投入运行,系统在运行过程中偶有交流滤波器短时间内频繁投切现象。作者分析了天广直流的无功控制及换流变分接头的控制方式,并应用带有天广直流真实模型的直流电磁暂态仿真程序(EM... 详细信息

天(生桥)—广(州)±500kV直流输电工程已于2001年6月双极投入运行,系统在运行过程中偶有交流滤波器短时间内频繁投切现象。作者分析了天广直流的无功控制及换流变分接头的控制方式,并应用带有天广直流真实模型的直流电磁暂态仿真程序(EMTDC)对故障记录数据进行了详细分析。分析结果表明,换流变分接头控制与无功控制中的不正确参数设置引发了交流滤波器的频繁投切。作者还提出了修改直流控制系统内部参数等切实可行的改进措施。

关键词： ±500kV天广直流输电系统交流滤波器无功控制策略电力系统

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Nonphytate Phosphorus Requirement and Efficacy of a Genetically Engineered Yeast Phytase for Yellow Broilers at 22- to 42-d-Old Age

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Agricultural Sciences in China 2011年第2期10卷 304-311页

作者： JIANG Zong-yong ZHOU Gui-lian LIN Ying-cai JIANG Shou-qun LI De-zhong xi peng-bin ZHANG Han-xing Key Laboratory of Animal Nutrition and Feed Science（South China） Ministry of Agriculture/Institute of Animal ScienceGuangdong Academy of Agricultural SciencesGuangzhou 510640P.R.China

An experiment was conducted to investigate the requirement of nonphytate phosphorus（nPP） and efficacy of a genetically engineered yeast phytase（PHY A） for Lingnan yellow broilers from 22-to 42-d-old age.A total of 1 320 1-d-old male chicks were randomly divided into 11 dietary treatment groups,which consisted of 4 replicate floor pens with 30 birds per *** control group（treatment 1） was fed with basal diet of nPP 0.08% without dicalcium phosphate or phytase *** levels of nPP were 0.16,0.24,0.32,0.40,0.48,and 0.56%,respectively,for treatments 2 to 7,through addition of dicalcium phosphate（chemistry grade） to the basal *** of treatments 8 to 11 were supplemented with PHY A at 200,400 and 600 U kg-1,a commercial phytase product（PHY B） at 400 U kg-1 level,*** birds in 0.32-0.56% nPP groups gained more than those of the other groups（P0.05）.The nPP supplementation significantly improved feed intake（P0.05）.The feed gain ratio was significantly decreased by 0.40% nPP diet compared to the control birds（P0.05）.The level of 0.48% nPP was required for optimum tibia *** additions of PHY A at 400 and 600 U kg-1 level and PHY B all significantly improved ADG（P0.05）,ADFI（P0.05）,and dry defatted tibia weight（P0.05）.Similarly,the percentage of tibia ash was increased by 600 U kg-1 PHY A supplementation（P0.05）.The requirement of nPP for maximal ADG and highest percentage tibia ash both was 0.40%.The phosphorus equivalency value of PHY A was estimated as 685 U kg-1 for male yellow broilers of 22-to 42-d-old age.

关键词： nonphytate phosphorus growth tibia ash genetically engineered yeast phytase phosphorus equivalency value yellow broilers

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重症烧伤患者能量消耗与需求平衡：值得深入研究的问题

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中华烧伤杂志 2013年第4期29卷 331-334页

作者：彭曦第三军医大学西南医院全军烧伤研究所创伤、烧伤与复合伤国家重点实验室重庆400038

经过近半个世纪的研究和探索，我国烧伤临床营养取得了长足进步，初步阐明了烧伤代谢规律，基本形成了较完善的营养治疗模式，一般烧伤患者能获得较满意的效果，但重症烧伤患者的疗效还不甚理想。重症烧伤患者一方面呈现持续的高分解状... 详细信息

经过近半个世纪的研究和探索，我国烧伤临床营养取得了长足进步，初步阐明了烧伤代谢规律，基本形成了较完善的营养治疗模式，一般烧伤患者能获得较满意的效果，但重症烧伤患者的疗效还不甚理想。重症烧伤患者一方面呈现持续的高分解状态，机体消耗增加，急需补给；同时又存在营养物质利用障碍，常规营养治疗难以逆转顽固的高分解代谢，其结果为机体自身消耗、自噬代谢等病理表现依旧。

关键词：烧伤高代谢分解代谢合成代谢能量需求能量消耗

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