检索结果-南通市图书馆

维普期刊数据库

Bioorthogonal chemistry in living animals

在线全文

学校读者我要写书评

暂无评论

National Science review 2017年第3期4卷 300-302页

作者： Xinyuan Fan Jie Li peng r.chen Beijing National Laboratory for Molecular Sciences Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education Synthetic and Functional Biomolecules Center College of Chemistry and Molecular Engineering Peking University Peking-Tsinghua Center for Life Sciences Peking University

Bioorthogonal chemistry refers to those chemical reactions that can be performed in living systems without interfering with native biological *** its first introduction by Bertozzi et *** 2003

关键词： in is that with Bioorthogonal chemistry in living animals been

Proximity Chemistry in Living Systems

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

CCS Chemistry 2023年第4期5卷 802-813页

作者： Yanjun Liu Yun Ge ruxin Zeng William Shu Ching Ngai Xinyuan Fan peng r.chen Synthetic and Functional Biomolecules Center Beijing National Laboratory for Molecular SciencesKey Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of EducationCollege of Chemistry and Molecular EngineeringPeking UniversityBeijing 100871 National Key Laboratory of Crop Genetic Improvement Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070 Shenzhen Bay Laboratory Institute of Chemical BiologyShenzhen 518107

Enzyme-and catalyst-generated reactive species have been leveraged in the past decade to covalently label biomolecules within a short range of a defined site or space inside cells or at the cell–cell *** to their high spatial resolution,such proximity labeling strategies have been coupled with various bioanalytical techniques for dissecting dynamic and complex biological ***,we review the development of enzyme-and catalyst-triggered proximity chemistry and their applications to identifying protein interaction networks as well as cell–cell communications in living systems.

关键词： proximity labeling bioorthogonal chemistry spatiotemporal proteomics cell-cell interactions living systems

Light-induced protein translocation by genetically encoded unnatural amino acid in Caenorhabditis elegans

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Protein & Cell 2013年第12期4卷 883-886页

作者： Hao Chang Mei Han Wenming Huang Guifeng Wei Juanjuan chen peng r.chen runsheng chen Junlong Zhang Tao Xu Pingyong Xu National Laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China College of Life Science and Technology Huazhong University of Science and Tech-nologyWuhan 430074China Laboratory of Non-coding rNA Institute of BiophysicsChinese Academy of SciencesBeijing 100101China College of Chemistry and Molecular Engi-neering Peking UniversityBeijing 100871China Key Laboratory of Interdisciplinary research Institute of BiophysicsChinese Academy of SciencesBeijing 100101China

Dear Editor,Living systems such as Caenorhabditis elegans are organized by interaction networks of biopolymer and small *** molecular engineering of specifi c targets to monitor and control these biological molecules has led to significant advances in our understand-ing of how biological systems are organ-ized,maintain themselves and disin-tegrate(Prescher and Bertozzi,2005).For instance,green fl uorescent protein(GFP)derived from jellyfi sh Aequorea Victoria helps us to accurately locate and observe proteins over long term *** addition,many novel LOV(light,oxy-gen,or voltage)domains such as LOV2-Jαand Vivid offer the possibility of cell-based motility management and tissue-based gene expression control(Wu et al.,2009;Wang et al.,2012).

关键词： instance maintain addition

维普期刊数据库

位点特异、拓扑结构明确的蛋白质-聚氨基酸偶联物的简洁合成

在线全文

学校读者我要写书评

暂无评论

高分子学报 2016年第11期26卷 1465-1467页

作者：陈鹏北京大学化学与分子工程学院北京100871

发展位点特异且具有明确拓扑结构的蛋白质-高分子偶联物是高分子和化学生物学领域共同面对的挑战之一.在聚合物末端精确引入一个或多个具有特殊反应活性的生物正交官能团是实现"位点特异"生物偶联的关键前提.这一过程通常比较低效、需... 详细信息

发展位点特异且具有明确拓扑结构的蛋白质-高分子偶联物是高分子和化学生物学领域共同面对的挑战之一.在聚合物末端精确引入一个或多个具有特殊反应活性的生物正交官能团是实现"位点特异"生物偶联的关键前提.这一过程通常比较低效、需要多步骤的官能团转化、聚合后修饰以及保护脱保护,费时且繁琐.最近,通过在聚合过程中原位生成官能团,以一锅-两步的过程得到可直接用于蛋白质偶联的异遥爪聚合物,从而实现了多种不同拓扑结构的蛋白质-聚氨基酸偶联物的快速构筑.这一简洁的合成路线实现了以前尚未获得的头-尾相接的环状偶联物的制备,使这些偶联物表现出了很强的体外酶稳定性以及热稳定性.该工作是蛋白质-高分子偶联化学的一次创新的尝试;同时,利用该方法所制备的偶联物在蛋白质药物领域具有广阔的应用前景.

关键词：简洁合成蛋白质-高分子偶联物聚氨基酸原位官能团生成

维普期刊数据库

Bioorthogonal Prodrug–Antibody Conjugates for On-Target and On-Demand Chemotherapy

在线全文

学校读者我要写书评

暂无评论

CCS Chemistry 2019年第2期1卷 226-236页

作者： Feng Lin Long chen Heng Zhang William Shu Ching Ngai Xiangmei Zeng Jian Lin peng r.chen Synthetic and Functional Biomolecules Center Beijing National Laboratory for Molecular SciencesCollege of Chemistry and Molecular EngineeringPeking UniversityBeijing 100871China Academy for Advanced Interdisciplinary Studies Peking UniversityBeijing 100871China Peking-Tsinghua Center for Life Sciences Peking UniversityBeijing 100871China

Current antibody–drug conjugates(ADCs)suffer from low tissue penetration and significant side effects,largely due to the permanent linkage and/or premature release of cytotoxic ***,we developed a prodrug–antibody conjugate(ProADC)strategy by conjugating a bioorthogonal-activatable prodrug with an antibody that allowed on-target release and on-demand activation of cytotoxic drugs at a tumor *** bioorthogonal-caged prodrug exhibited an enhanced permeability into and on-demand activation within cancer cells,while the pH-sensitive ADC linker allowed on-target release of the anticancer ***,the ProADCs showed enhanced tumor penetration and alleviated side effects for use as an on-target and on-demand chemotherapy agents.

关键词： antibody-drug conjugates bioortho-gonal prodrug iEDDA reaction enhanced tumor penetration alleviated side effects

Site-specific dual labeling of EGFr in living cells

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Site-specific dual labeling of EGFR in living cells

第八届全国化学生物学学术会议

作者： Yi Yang peng r.chen Department of Chemical Biology College of Chemistry and Molecular EngineeringPeking University

＜正＞Epidermal growth factor（EGF）receptor is a tyro sine kinase which regulates cell growth and differentiation and is implicated in many human *** vivo site-specific labeling of fluorescent or functional probes on... 详细信息

关键词： EGFr dual labeling click chemistry lipoic acid ligase

The application of Cu-mediated bioothogonal cleavage reaction in living system

在线全文

cnki会议

学校读者我要写书评

暂无评论

The application of Cu-mediated bioothogonal cleavage reactio...

第十届全国化学生物学学术会议

作者： Xin Wang Yanjun Liu peng r.chen Beijing National Laboratory for Molecular Sciences Synthetic and Functional Biomolecules CenterDepartment of Chemical BiologyCollege of Chemistry and Molecular EngineeringPeking University

Bioorthogonal reaction pairs are playing more and more important role in chemical biology. Compared to traditional bioorthogonal coupling reaction, the biorthogonal cleavage reaction is brought into focus in recent years. So in this work, we developed a copper-mediated bioorthogonal cleavage pair. Then we expended this pair on activation of prodrug, unnatural amino acid for protein activation in living cells. At last, we proposed that it could be as a linker of antibody-drug conjugate for cancer therapy in living animals.

关键词： copper bioorthogonal cleavage reaction unnatural amino acid

Site-specifically incorporated and decaged photo-affinity lysine to capture histone–effector interactions in mammalian cells

在线全文

cnki会议

学校读者我要写书评

暂无评论

Site-specifically incorporated and decaged photo-affinity ly...

第十一届全国化学生物学学术会议

作者： Lu Liu Gong Zhang peng r.chen College of Chemistry and Molecular Engineering Peking University Peking-Tsinghua Center for Life Sciences Peking University

Post-translational modifications（PTMs） on lysine residues are essential histone marks for dynamic regulation of chromatin structure and gene transcription. Among them, unmodified lysine at certain site（s） represents a unique histone mark that can be preferentially recognized by effector proteins that control transcriptional regulations. To identify such effectors, we herein developed a chemically caged photo-affinity lysine precursor（caged K*） that can be genetically incorporated and enzymatically decaged at a given histone site in mammalian cells. By coupling the genetic code expansion technique with bioorthogonal enzyme decaging and subsequent photo-crosslinking, our strategy allowed the site-specific photo-affinity capture of unmodified lysine-mediated histone–effector interactions in living cells.

关键词： histone–effector interactions photo-affinity lysine unmodified histone enzyme-triggered decaging

rapid kinase decaging coupled with phosphotyrosine proteomics enables cellular signal pulse-chase

在线全文

cnki会议

学校读者我要写书评

暂无评论

Rapid kinase decaging coupled with phosphotyrosine proteomic...

第十一届全国化学生物学学术会议

作者： Yicheng Weng Wendong chen ruijun Tian peng r.chen Academy for Advanced Interdisciplinary Studies Peking University Departments of Chemistry Southern University of Science and Technology College of Chemistry and Molecular Engineering Peking University

Tyrosine phosphorylation(p-Tyr) is a canonical form of cellular signaling transduction,deregulation of which can drive cancer ***,studying p-Tyr mediated signaling can promote understanding disease development mechanism and drug *** kind of signal transduction usually takes tens of seconds to several minutes,which can be a challenge when p-Tyr signaling is studied in a cellular ***,we designed a general strategy to dissect the p-Tyr signaling downstream tyrosine *** kinase was expressed in an inactive state,where the critical lysine residue,conserved in the catalytic pocket of kinases,was caged by a photo-sensitive group with genetic code expansion *** caging group could be rapidly removed by 375 nm ultraviolet light illumination,releasing the native functional *** with p-Tyr superbinder enrichment enabled p-Tyr proteomics,the dynamic of was captured in a high temporal *** within an extended incubation,the downstream p-Tyr events were chased.9514 high-confidential p-Tyr sites were quantified,revealing the unexpected signaling disturbation of caused a single kinase activation.

关键词： Kinase decaging tyrosine phosphoproteomics second-scale temporal resolution