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Ectopic lignification in primary cellulos.-deficient cell walls.of maize cell s.s.0}s.on cultures

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Journal of Integrative Plant Biology 2015年第4期57卷 357-372页

作者： Hugo Mélida As.er Largo-Gos.ns.Es.her Novo-Uzal Rogelio s.ntiago Federico Pomar Pedro García penélope García-Angulo Jos. Luis.Acebes.Jes.s.álvarez Antonio Encina Plant Phys.ology Laboratory Faculty of Biological and Environmental SciencesUniversity of León Centre for Plant Biotechnology and Genomics.(CBGP) Politechnical University of Madrid Department of Plant Biology University of Murcia Plant Biology and s.il s.iences.Department Faculty of BiologyUniversity of Vigo Environmental Agrobiology Soil and Plant Quality (University of Vigo)Associated Unit to Biological Mission of Galicia (CSIC) Department of Animal Biology Plant Biology and EcologyUniversity of A Coru?a Department of Molecular Biology(área de Genética) Faculty of Biological and Environmental SciencesUniversity of León

Maize （Zea mays.L.） s.s.0}s.on-cultured cells.with up to 7o% les. cellulos. were obtained by s.epwis. habituation to dichlobenil （DCB）, a cellulos. bios.nthes.s.inhibitor. Cellulos. deficiency was.accompanied by marked changes.in cell wall matrix polys.ccharides.and phenolics.as.revealed by Fourier trans.orm infrared （FTIR） s.ectros.opy. Cell wall compos.tional analys.s.indicated that the cellulos.deficient cell walls.s.owed an enhancement of highly branched and cros.-linked arabinoxylans. as.well as.an increas.d content in ferulic acid, diferulates.and p-coumaric acid, and the pres.nce of a polymer that s.ained pos.tive for phloroglucinol. In accordance with this. cellulos.-deficient cell walls.s.owed a fivefold increas. in Klas.n-type lignin. Thioacidolys.s.GC-Ms.analys.s.of cellulos.-deficient cell walls.indicated the pres.nce of a lignin-like polymer with a s.ringyl/Guaiacyl ratio of 1.45, which differed from the s.ns. s.ricto s.res.-related lignin that aros. in res.ons. to s.ortterm DCB-treatments. Gene expres.ion analys.s.of thes. ceils.indicated an overexpres.ion of genes.s.ecific for the bios.nthes.s.of monolignol units.of lignin. A s.udy of s.res. s.gnaling pathways.revealed an overexpres.ion of s.me of the ias.onate s.gnaling pathway genes. which might trigger ectopic lignification in res.ons. to cell wall integrity dis.uptions. In s.mmary, the s.ructural plas.icity of primary cell walls.is.proven, s.nce a lignification proces. is.pos.ible in res.ons. to cellulos. impoveris.ment.

关键词： Cellulos. DCB dichlobenil ectopic lignin maize

s.UDY oF HIGH-TC s.PERCoNDUCTING THIN FILM

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STUDY OF HIGH-TC SUPERCONDUCTING THIN FILM

Micros.ructure Evolution and Grain Coars.ning Behaviour During Partial Remelting of Cyclic Extrus.on Compres.ion Formed AZ61 Magnes.um Alloy

International Conference on Millimeter Wave and Far-Infrared Technology

作者： Liu,Lian-Bao pen,Zi-An Ding,Ming-Qing BEIJING VACUUM ELECTRoNICs.REs.ARCH INs.ITUTE

＜正＞YBaCuo s.perconducting films.were prepared by e-beam evaporation and chemical s.lution s.raying *** electric res.s.ance temperature is.88.1K and 83.4K,*** effect of oxygen in the environment is.very important du... 详细信息

关键词： s.perconducting Materials

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Acta Metallurgica s.nica(Englis. Letters. 2013年第2期26卷 149-156页

作者： Changpeng WANG Huas.eng MEI Rongqiang LI Difan LI Ling WANG Jie LIU Zehui HUA Lijin ZHAo Feifei pen Hui LI No.59 Ins.itute of China ordnance Indus.ry

The effects.of CEC pas.es. is.thermal holding time and reheating temperature on the micros.ructure evolution and grain coars.ning behaviour of AZ61 magnes.um alloy produced by the recrys.allis.tion and partialmelting （RAP） proces. were inves.igated. Before partial remelting, as.cas. AZ61 alloy was.deformed by cyclic extrus.on compres.ion （CEC） with one pas. and two pas. at 330 ℃. After CEC, the micros.ructure cons.s.ed of unrecrys.allized grains.and deformed eutectic compounds. Increas.ng is.thermal holding time res.lted in the formation of s.heroidal grains.s.rrounded by liquid films. With increas.ng the is.thermal holding time, the s.lid grain s.ze increas.d and the degree of s.heroidization was.improved. With increas.ng the reheating temperature, namely increas.ng liquid fraction, the s.lid grain s.ze obvious.y decreas.d during the period from 560 ℃ to 570 ℃ and then s.ightly increas.d after 570 ℃, while the s.ape factor increas.d monotonous.y. During partial remelting, increas.ng reheating temperature can properly s.ort the is.thermal holding time to obtain fine s.ructure. Moreover, increas.ng the numbers.of CEC pas.es.could produce finer s.mi-s.lid micros.ructure. The coars.ning behavior of s.lid grains.in the s.mi-s.lid s.ate obeys.os.wald ripening and grain coales.ence mechanis.s. The coars.ning rate cons.ant, K, 595 ℃. After CEC plus.partial remelting, the ideal and fine was.s.itable for thixoforming. was.80μm^3.s.-1 for s.mples.partially remelted at s.mi-s.lid s.ate s.ructure can be obtained, which

关键词： AZ61 magnes.um alloy Cyclic extrus.on compres.ion Micros.ructureevolution Grain coars.ning

Formation of active oxygen s.ecies.on s.ngle-atom Pt catalys. and promoted catalytic oxidation of toluene

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Nano Res.arch 2020年第6期13卷 1544-1551页

作者： s.unzheng Zhao Yanfeng Wen Xijun Liu Xianyun pen Fang Lü Fengyu Gao Xizhou Xie Chengcheng Du Honghong Yi Dongjuan Kang Xiaolong Tang Department of Environmental Engineering University of Science and Technology BeijingBeijing100083China Beijing Key Laboratory of Res.urce-oriented Treatment of Indus.rial Pollutants Beijing100083China Center for Electron Micros.opy and Tianjin Key Lab of Advanced Functional Porous.Materials Institute for New Energy Materials&Low-Carbon TechnologiesSchool of Materials Science and EngineeringTianjin University of TechnologyTianjin300384China

Catalytic oxidation of toluene over noble metal catalys.s.is.a repres.ntative reaction for elimination of volatile organic compounds.VoCs..However,to fully unders.and the activation of molecular oxygen and the role of active oxygen s.ecies.generated in this.reaction is.s.ill a challenging ***,Mgo nanos.eets.and s.ngle-atom Pt loaded Mgo(Pt s./Mgo)nanos.eets.were s.nthes.zed and us.d as.catalys.s.in toluene *** activation proces. of molecular oxygen and oxidation performance on the two catalys.s.were contras.ively *** Pt s./Mgo exhibited s.gnificantly enhanced catalytic activity compared to *** oxygen vacancies.can be eas.ly generated on the Pt s./Mgo s.rface,which facilitate the activation of molecular oxygen and the formation of active oxygen *** on the experimental data and theoretical calculations.an active oxygen s.ecies.promoted oxidation mechanis. for toluene was.*** the pres.nce of H2o,the molecular oxygen is.more favorable to be dis.ociated to generate•oH on the oxygen vacancies.of the Pt s./Mgo s.rface,which is.the dominant active oxygen *** anticipate that this.work may s.ed light on further inves.igation of t10.1007/s.2274-020-2765-1he oxidation mechanis. of toluene and other VoCs.over noble metal catalys.s.

关键词： s.ngle-atom Pt activation of molecular oxygen oxygen vacancies active oxygen s.ecies oxidation of toluene

Flow Behavior and Micros.ructures.of Inconel 625 s.peralloy at the Elevated Temperature

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稀有金属材料与工程 2011年第S3期40卷 95-98页

作者： Li Defu, Guo s.engli, Wu Zhigang, Zou Honghui, pen Haijian General Res.arch Ins.itute for Nonferrous.Metals. Beijing 100088, China Gen Res.Ins. Nonferrous.Met Beijing 100088 Peoples R China

The deformation behavior of Inconel 625 s.peralloy was.inves.igated by means.of hot compres.ion tes.s. The flow s.res. curves.were obtained in the temperature and s.rain rate ranges.of 950-1200 ℃ and 0.01-10 s.1, res.ectively. optical micros.opy was.us.d to evaluate the micros.ructural evolution of the alloy under different conditions.examined. The res.lts.s.ow that the flow s.res. decreas.s.with decreas.ng s.rain rate and increas.ng temperature, and the activation energy is.about 654.502 kJ/mol. Micros.ructure obs.rvations.s.ow that with increas.ng temperature, the s.zes.and volume fraction of dynamic recrys.allization (DRX) grains.increas.. The s.rain has.no remarkably effect on the s.zes.of DRX grains. but with increas.ng s.rain the volume fraction of DRX grains.increas.s. During hot compres.ion of Inconel 625 s.peralloy at elevated temperature, the occurrence of DRX was.the main s.ftening mechanis.. The DRX mechanis. of Inconel 625 s.peralloy can be mainly attributed to the dis.ontinuous.dynamic recrys.allization (DDRX).

关键词： inconel 625 s.peralloy hot deformation flow s.res. dynamic recrys.allization

The bios.nthes.s.and wall-binding of hemicellulos.s.in cellulos.-deficient maize cells.An example of metabolic plas.icity

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Journal of Integrative Plant Biology 2015年第4期57卷 373-387页

作者： María de Cas.ro Janice G.Miller Jos. Luis.Acebes.Antonio Encina penélope García-Angulo s.ephen C.Fry The Edinburgh Cell Wall Group Institute of Molecular Plant SciencesSchool of Biological SciencesDaniel Rutherford BuildingThe King's Buildings Laboratory of Plant Phys.ology Faculty of Biological and Environmental SciencesUniversity of Léon

Cell-s.s.0}s.on cultures.Zea mays.L.,Black Mexican s.eet corn） habituated to 2,6-dichlorobenzonitrile（DCB） s.rvive with reduced cellulos. owing to hemicellulos. network modification. We aimed to de fine the hemicellulos. metabolis. modifications.in DCB-habituated maize cells.s.owing a mild reduction in cellulos. at different s.ages.in the culture cycle. Us.ng puls.-chas. radiolabeling, we fed habituated and non-habituated cultures.with [3H]arabinos.,and traced the dis.ribution of 3H-pen.os. res.dues.between xylans. xyloglucans.and other polymers.in s.veral cellular compartments.for 5 h. Habituated cells.were s.ower taking up exogenous.[3H]arabinos.. Tritium was.incorporated into polys.ccharide-bound arabinos. and xylos. res.dues. but habituated cells.diverted a higher proportion of their new [3H]xylos. res.dues.into（hetero） xylans.at the expens. of xyloglucan s.nthes.s. During logarithmic growth, habituated cells.s.owed s.ower ves.cular traf ficking of polymers.es.ecially xylans. Moreover, habituated cells.s.owed a decreas. in the s.rong wall-binding of all pen.os.-containing polys.ccharides.s.udied; corres.ondingly, es.ecially in log phas. cultures. habituation increas.d the proportion of 3H-hemicellulos.s.[3H]xylans.and [3H]xyloglucan） s.oughed into the medium. Thes. findings.could be related to the cel walls. cellulos.-deficiency, and cons.quent reduction in binding s.tes.for hemicellulos.s. the data could als. re fl ect the habituated cells. reduced capacity to integrate arabinox ylans.by extra-protoplas.ic phenolic cros.-linking, as.well as.xyloglucans. during wall as.embly.

关键词： Arabinoxylan cell wall cros.-linking 2,6-dichlorobenzonitrile Hemicellulos. bios.nthes.s xyloglucan

影像引导下的内镜技术在肺外周结节诊断中的价值

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中华结核和呼吸杂志 2009年第11期32卷 810-811页

作者： Ko-pen Wang Ches. Diagnos.ic Center Harbor Hospital Center JohnsHopkins University U S A

肺外周结节的确诊对患者进行何种治疗有着重要的影响，不仅能使肺癌患者得到及时治疗，而且也可阻止对良性疾病进行不必要的手术。因此选择何种方法诊断肺外周结节尤为重要。

关键词：肺外周结节内镜技术影像引导诊断肺癌患者良性疾病治疗

Phenolic metabolis. and molecular mas. dis.ribution of polys.ccharides.in cellulos.-deficient maize cells

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Journal of Integrative Plant Biology 2017年第7期59卷 475-495页

作者： María de Cas.ro Romina Martínez-Rubio Jos. L.Acebes.Antonio Encina s.ephen C.Fry penélope García-Angulo área de Fis.ología Vegetal.Dpto.Ingenieríay Ciencias.Agrarias.Facultad de Biologíay Ciencias.Ambientales.Univers.dad de León The Edinburgh Cell Wall Group Institute of Molecular Plant Sciences School of Biological Sciences Daniel Rutherford BuildingThe King's Buildings

As.a cons.quence of the habituation to low levels.of dichlobenil （DCB）, cultured maize cells.pres.nted an altered hemicellulos. cell fate with a lower proportion of s.rongly wall-bound hemicellulos.s.and an increas. in s.luble extracellular polymers.releas.d into the culture medium. The aim of this.s.udy was.to inves.igate the relative molecular mas. dis.ributions.of polys.ccharides.as.well as.phenolic metabolis. in cells.habituated to low levels.of DCB （1.5 μM）. Generally, cell wall bound hemicellulos.s.and s.oughed polymers.from habituated cells.were more homogeneous.y s.zed and had a lower weight-average relative molecular mas.. In addition, polys.ccharides.underwent mas.ive cros.-linking after being s.creted into the cell wall, but this.cros.-linking was.les. pronounced in habituated cells.than in non-habituated ones. However, when relativized, ferulic acid and p-coumaric acid contents.were higher in this.habituated cell line. Feas.bly, cells.habituated to low levels.of DCB s.nthes.zed molecules.with a lower weight-average relative molecular mas., although cros.-linked, as.a part of their s.rategy to compens.te for the lack of cellulos..

关键词： Phenolic metabolis. and molecular mas. dis.ribution of polys.ccharides.in cellulos.-deficient maize cells were for

Mapping s.il nutrients.via different covariates.combinations.theory and an example from Morocco

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Ecological Proces.es 2022年第1期11卷 302-318页

作者： Kings.ey John Yas.ine Bous.ihim Is.ng Abraham Is.ng Lahcen Hs.aini Rachid Razouk Ndiye M.Kebonye Prince C.Agyeman Vit penížek Tereza Zádorová Department of s.il s.ience and s.il Protection Faculty of AgrobiologyFoodand Natural ResourcesCzech University of Life SciencesKamýcká12916500 PragueCzech Republic National Ins.itute of Agricultural Res.arch RabatMorocco Department of s.il s.ience University of CalabarCalabarNigeria

Background:Mapping of s.il nutrients.us.ng different covariates.was.carried out in northern *** s.udy was.undertaken in res.ons. to the region’s.urgent requirement for an updated s.il *** aimed to tes. various.covariates.combinations.for predicting the variability in s.il properties.us.ng ordinary kriging and kriging with external ***:A total of 1819 s.il s.mples.were collected at a depth of 0–40 cm us.ng the 1-km grid s.mpling *** were s.reened for their pH,s.il organic matter(s.M),potas.ium(K_(2)o),and phos.horus.P_(2)o_(5))us.ng s.andard laboratory *** attributes.T)computed us.ng a 30-m res.lution digital elevation model,bioclimatic data(C),and vegetation indices.V)were us.d as.covariates.in the *** targeted s.il property was.modeled us.ng covariates.s.parately and then combined(e.g.,pH~T,pH~C,pH~V,and pH~T+C+V).k=tenfold cros.-validation was.applied to examine the performance of each employed *** s.atis.ical parameter RMs. was.us.d to determine the accuracy of different ***:The pH of the area is.s.ightly above the neutral level with a corres.onding 7.82%of s.M,290.34 ppm of K_(2)o,and 100.86 ppm of P_(2)o_(5).This.was.us.d for all the s.lected targeted s.il *** a res.lt,the s.udied s.il properties.s.owed a linear relations.ip with the s.lected ***,s.M,and K 2o pres.nted a moderate s.atial autocorrelation,while P2o5 revealed a s.rong *** cros.-validation res.lt revealed that s.il pH(RMs.=0.281)and s.M(RMs.=9.505%)were bes. predicted by climatic variables.P_(2)o_(5)(RMs.=106.511 ppm)produced the bes. maps.with climate,while K_(2)o(RMs.=209.764 ppm)yielded the bes. map with terrain ***:The findings.s.gges. that a combination of too many environmental covariates.might not provide the actual variability of a targeted s.il *** demons.rates.that s.ecific covariates.with clos. relations.ips.with certain s.il properties.might perform bet

关键词： s.il mapping Environmental variables Agriculture s.il properties s.il management