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Reference ranges and age-related changes of peripheral blood lymphocyte subsets in chinese healthy adults

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Science China(Life sciences) 2009年第7期52卷 643-650页

作者： JIAO Yang1,QIU ZhiFeng1,XIE Jing1,LI DongJing2 & LI TaiSheng1 1 Department of Infectious Disease,peking union medical college Hospital,peking union medical college & chinese academy of medical sciences,Beijing,China 2 Department of Special Care Unit,peking union medical college Hospital,peking union medical college & chinese academy of medical sciences,Beijing,China Department of Infectious Disease Peking Union Medical College Hospital Peking Union Medical College & Chinese Academy of Medical Sciences Beijing China Department of Special Care Unit Peking Union Medical College Hospital Peking Union Medical College & Chinese Academy of Medical Sciences Beijing China

This study was performed to build region-specific reference ranges of peripheral blood lymphocyte subsets for chinese healthy adults from the young to the elderly and analyze the trends of changes in lymphocyte subsets for evaluating the impact of age on the values.151 healthy adults aged 19-86 were recruited based on the SENIEUR *** sets of reference ranges were finally built applicable for the healthy young(19-44 years),middle-aged(45-64 years) and elder adults(≥65).Comparisons in parameters among the three cohorts showed that a statistically significant increase in CD16CD56+ NK cell was observed between the middle-aged and elder cohorts,whereas for the majority of the parameters,a significant decline was observed between the young and the middle-aged *** results showed that inverse correlations were observed between the age and CD19+ B,CD3+ T,CD3+CD4+ T,CD4+CD45RA+CD62L+ nave T cell and CD4+CD28+/CD4+,while the positive one was identified between the age and the NK *** significant changes of the most of immune parameters provided evidence for ***,T cell activation markers of CD8+CD38+ and CD8+HLA-DR+ showed reverse trends of association with age,which provides a clue for further researches on the mechanisms underlying the paradoxical clinical presentation of the elder patients.

关键词： lymphocyte subsets age reference range immunosenescence

Liu Shih-Hao:Pioneer of translational medicine in China

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Science China(Life sciences) 2011年第12期54卷 1089-1095页

作者： LI NaiShi Department of Endocrinology,peking union medical college Hospital,peking union medical college,chinese academy of medical sciences Key Laboratory of Endocrinology,Ministry of Health of China,Beijing 100730,China 1. Department of Endocrinology Peking Union Medical College Hospital Peking Union Medical College Chinese Academy of Medical Sciences Beijing China

Editorial comment Translational medicine is a new discipline which aims to eliminate the barrier between preclinical and clinical medicine. Here, Dr. Li discusses the application of translational medicine in the research, teaching and clinical work of Prof. Liu Shih-Hao, the founder of endocrinology in China, who was particularly renowned for his early work in calcium and phosphorus metabolism. This well-known success story can be traced back to an early appreciation of translational medicine by Prof. Liu Shih-Hao, and serves as an important and revelatory lesson for us all.

关键词： translational medicine Liu Shih-Hao

Total Synthesis of (±)-Cordatolide A and its Anti-HIV Activity

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chinese Chemical Letters 1999年第8期10卷 653-656页

作者： Qi GAO Lin WANG Xiao Tian LIANG (Institute of Materia Medica, peking union medical college & chinese academy of medical sciences, Beijing 100050) Institute of Materia Medica Peking Union Medical College Chinese Academy of Medical Sciences Beijing 100050 China

The natural product (±)-cordatolide A has been synthesized by a four-step approach starting from phloroglucinol, including Pechmann reaction, Friedel-Crafts acylation, cyclization. chromenylation and Luche reduction.

关键词： Total synthesis (±)-cordatolide A

Synthesis of 11-demethyl and 6, 6,11-demethyl Calanolide A

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chinese Chemical Letters 1997年第10期8卷 859-860页

作者： Chun Mei ZHOU Lin WANG Zhi Zhong ZHAO (Institute of Materia Medica, peking union medical college & chinese academy of medical sciences, Bejing, 100050) chinese ACAD MED SCI BEIJING 100050PEOPLES R CHINA BEIJING union MED COLL INST MAT MEDBEIJING 100050PEOPLES R CHINA

Synthesis of 11-demethyl and 6, 6, 11-demethyl calanolides A (6-9) have been carried out by a four-step reaction sequence using a simple approach in order to investigate the structural requirements necessary for antiv... 详细信息

关键词： ppm HNMR MHz Synthesis of 11-demethyl and 6 demethyl Calanolide A

SYNTHESIS　OF　POLYSUBSTITUTED　AROMATIC　COMPOUNDS　***　DIELS-ALDER　REACTION　OF　PHENYLSELENO　SUBSTITUTED　DIENES　WITH　DIMETHYL　ACETYLENEDICARBOXYLATE

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chinese Chemical Letters 1995年第9期6卷 769-770页

作者： Guang Dlan HAN Guo Feng HUANG(Institute of Materia Medica, peking union medical college, chinese academy of medical sciences, Beijing 100060) chinese Acad Med Sci Inst Mat Med Peking Union Med Coll Beijing 100050 Peoples R China

Abstract: The phenylseleno substituted aromatlc compounds were prepared via the Diels-Alder reaction of phenylseleno substituted dlenes with dimethyl acetylenedicarboxylate followed by DDQ dehydrogenation.

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Assay of mitochondrial functions by resazurin in vitro

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Acta Pharmacologica Sinica 2004年第3期25卷 131-135页

作者： Hai-xia ZHANG,Guan-hua DU2,Jun-tian ZHANG Institute of Materia Medica,peking union medical college & chinese academy of medical sciences,Beijing100050,China Institute of Materia Medica Peking Union Medical College & Chinese Academy of Medical SciencesBeijing 100050 China

AIM: To study the mechanism of resazurin as indicator of mitochondrial function and to develop a rapid and sensitive assay for measuring metabolic activity of isolated mitochondria from rat liver in vitro. METHODS: The screening was carried out on 96-well microtitre plates by monitoring fluorescence intensity of resazurin reduced by mitochondria. Experimental conditions were optimized and influences of several inhibitors on mitochondrial func- tion were observed. RESULTS: Fluorescence intensity increased in a linear manner when the mitochondrial protein concentration from 5 to 50 μg protein per well was incubated with resazurin (5 μmol/L) during 230 min period at 37 oC. Edetic acid could promote the reduction of resazurin in mitochondria. The fluorescence intensity decreased greatly after pretreatment with NaN3, antimycin A, carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP), and oligomycin compared with the control. However, the typical complex I inhibitor, rotenone enhanced the fluorescence intensity without mitochondria. CONCLUSION: Using resazurin to determine mitochondrial func- tion is sensitive, inexpensive and could be easily automated for high throughput screening.

关键词： resazurin drug screening mitochondria

A Convenient Method for the Stereoselective Synthesis of 7αHydroxycholesterol

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chinese Chemical Letters 1998年第8期9卷 715-716页

作者： Ling Jie GAO and Tian Zeng ZHAO Guang Dian HAN(The Institute of Chemistry, Henan academy of sciences, Zhengzhou, 450002)(Institute of Materia Medica, peking union medical college,chinese academy of medical sciences, Beijing 100050) Henan Acad Sci Inst Chem Zhengzhou 450002 Peoples R China chinese Acad Med Sci Inst Mat Med Oeking Union Med Coll Beijing 100050 Peoples R China

7α-Hydroxycholesterol was prepared from cholesteryl acetate via allylic browhnation followed by hydroxylation in 73% overall yield.

关键词： 7α-hyrdoxycholesterol cholesteryl acetate stereoselective hydroxylation

Synthesis of 2S-Hydroxymethyl-3R, 4R-dihydroxypyrrolidine

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chinese Chemical Letters 1999年第8期10卷 651-652页

作者： Zhen CHEN Ren Yun WANG Li Lian ZHU Xiao Tian LIANG (Institute of Materia Medica. peking union medical college) (The chinese academy of medical sciences, Bejing 100050) chinese Acad Med Sci Inst Mat Med Peking Union Med Coll Beijing 100050 Peoples R China

The sythesis of 2S-hydroxymethyl-3R, 4R-dihydroxypyrrolidine from D-arabinose was described in this paper.

关键词： polydroxylated pyrrolidine 2S-hydroxymethyl-3R 4R-dihydroxypyrrolidine D-arabinose

Immunobiology of Facial Nerve Repair and Regeneration

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Journal of Otology 2006年第2期1卷 107-115页

作者： QUAN Shi-ming, GAO Zhi-qiang Department of Otorhinolaryngology, peking union medical college Hospital, chinese academy of medical sciences and peking union medical college, Beijing People’s Republic, China Department of Otorhinolaryngology Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing People’s Republic China

Immunobiological study is a key to revealing the important basis of facial nerve repair and regeneration for both research and development of clinic treatments. The microenvironmental changes around an injuried facial motoneuron, i.e., the aggregation and expression of various types of immune cells and molecules in a dynamic equilibrium, impenetrate from the start to the end of the repair of an injured facial nerve. The concept of 'immune microenvironment for facial nerve repair and regeneration', mainly concerns with the dynamic exchange between expression and regulation networks and a variaty of immune cells and immune molecules in the process of facial nerve repair and regeneration for the maintenance of a immune microenvironment favorable for nerve repair. Investigation on microglial activation and recruitment, T cell behavior, cytokine networks, and immunological cellular and molecular signaling pathways in facial nerve repair and regeneration are the current hot spots in the research on immunobiology of facial nerve injury. The current paper provides a comprehensive review of the above mentioned issues. Research of these issues will eventually make immunological interventions practicable treatments for facial nerve injury in the clinic.

关键词： microglia T cell cytokine network microenviroment signaling pathway repair and regeneration facial nerve