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Differential regulation of H3S10 phosphorylation, mitosis progression and cell fate by Aurora Kinase B and C in mouse preimplantation embryos

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Protein & Cell 2017年第9期8卷 662-674页

作者： Wenzhi Li peizhe wang Bingjie Zhang Jing Zhang Jia Ming Wei Xie Jie Na Center for Stem Cell Biology and Regenerative Medicine School of Medicine Tsinghua University Beijing 100084 China Center for Stem Cell Biology and Regenerative Medicine MOE Key Laboratory of Bioinformatics THU-PKU Center for LifeSciences School of Life Sciences Tsinghua University Beijing 100084 China

Coordination of cell division and cell fate is crucial for the successful development of mammalian early embryos. Aurora kinases are evolutionarily conserved serine/threonine kinases and key regulators of mitosis. Aurora kinase B （AurkB） is ubiquitously expressed while Aurora kinase C （AurkC） is specifically expressed in gametes and preimplantation embryos. We found that increasing AurkC level in one blastomere of the 2-cell embryo accelerated cell division and decreasing AurkC level slowed down mitosis. Changing AurkB level had the opposite effect. The kinase domains of AurkB and AurkC were responsible for their different ability to phosphorylate Histone H3 Serine 10 （H3S10P） and regulate metaphase timing. Using an Oct4-photoactivat- able GFP fusion protein （Oct4-paGFP） and fluorescence decay after photoactivation assay, we found that AurkB overexpression reduced Oct4 retention in the nucleus. Finally, we show that blastomeres with higher AurkC level elevated pluripotency gene expression, which were inclined to enter the inner cell mass lineage and subsequently contributed to the embryo proper. Collectively, our results are the first demonstration that the activity of mitotic kinases can influence cell fate decisions in mammalian preimplantation embryos and have important implications to assisted reproduction.

关键词： Aurora kinase mouse preimplantation embryo cell fate development mitosis

Dgcr8 deletion in the primitive heart uncovered novel microRNA regulating the balance of cardiac-vascular gene program

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Protein & Cell 2019年第5期10卷 327-346页

作者： Xi Chen Lin wang Rujin Huang Hui Qiu peizhe wang Daren Wu Yonglin Zhu Jia Ming Yangming wang Jianbin wang Jie Na Center for Stem Cell Biology and Regenerative Medicine School of MedicineTsinghua UniversityBeijing 100084China Beijing Key Laboratory of Cardiometabolic Molecular Medicine Institute of Molecular MedicinePeking UniversityBeijing 100871China School of Life Sciences Tsinghua UniversityBeijing 100084China

Primitive mammalian heart transforms from a single tube to a four-chambered muscular organ during a short developmental *** found that knocking out global microRNA by deleting Dgcr8 microprocessor in Mespl cardiovascular progenitor cells lead to the formation of extremely dilated and enlarged heart due to defective cardiomyocyte(CM)*** analysis revealed unusual upregulation of vascular gene expression in Dgcr8 cKO *** cell RNA sequencing study further confirmed the increase of angiogenesis genes in single Dgcr8 cKO *** also performed global microRNA profiling of E9.5 heart for the first time,and identified that miR-541 was transiently highly expressed in E9.5 ***,introducing miR-541 back into microRNA-free CMs partially rescued their defects,downregulated angiogenesis genes and significantly upregulated cardiac ***,miR-541 can target Ctgf and inhibit endothelial *** results suggest that micro-RNAs are required to suppress abnormal angiogenesis gene program to maintain CM differentiation.

关键词： microRNA Dgcr8 Cardiovascular progenitor cells miRNA-541 Single cell RNA sequencing

基于CT的影像组学在胃肠道间质瘤中的应用进展

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老年医学研究 2022年第3期3卷 38-41页

作者：王沛哲闫呈新山东第一医科大学第二附属医院影像科山东泰安271000

胃肠道间质瘤是一种常见的消化系统间叶源性肿瘤,其临床症状与影像学表现特异性不高,故较难进行诊断与鉴别诊断。传统的影像学特征较难分辨,近年提出的基于CT的影像组学可以运用大数据分析的方法在医学影像图像中高通量地提取图像的特征... 详细信息

胃肠道间质瘤是一种常见的消化系统间叶源性肿瘤,其临床症状与影像学表现特异性不高,故较难进行诊断与鉴别诊断。传统的影像学特征较难分辨,近年提出的基于CT的影像组学可以运用大数据分析的方法在医学影像图像中高通量地提取图像的特征,来量化鉴别肿瘤,可以用于胃肠道肿瘤的鉴别诊断、病理危险度分级的预测和评估以及预测肿瘤细胞的增殖状态。

关键词：体层摄影术 CT 影像组学胃肠道肿瘤危险度分级

基于GRU神经网络的数据中心能耗预测模型研究

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电力信息与通信技术 2021年第3期19卷 10-18页

作者：杨丽娜赵鹏王佩哲西安交通大学电子与信息学部计算机学院陕西西安710049

数据中心产生的巨大能耗给经济和环境带来了压力,对能耗进行分析和预测可为其能耗效率的优化提供重要依据。考虑到包括室外天气、内部数据中心CPU负载等影响数据中心能耗的复杂因素,文章提出了基于门控循环单元(Gated Recurrent Unit,G... 详细信息

数据中心产生的巨大能耗给经济和环境带来了压力,对能耗进行分析和预测可为其能耗效率的优化提供重要依据。考虑到包括室外天气、内部数据中心CPU负载等影响数据中心能耗的复杂因素,文章提出了基于门控循环单元(Gated Recurrent Unit,GRU)神经网络的数据中心能耗预测模型。首先,通过对数据中心能耗数据进行分析,提取了与数据中心能耗最强相关的特征,并将这些时序特征数据作为输入进行模型训练。然后,提出一种结合神经网络(Artificial Neural Network,ANN)和GRU的网络模型(ANN-GRU)来预测数据中心能耗。最后,在仿真环境下基于真实轨迹数据进行了验证和分析。结果表明,与支持向量回归(Support Vector Regression,SVR)、LinearSVR、ANN模型相比,ANN-GRU具有更高的预测精度。

关键词：数据中心能耗预测时间序列数据人工神经网络 GRU

A review of extractive distillation from an azeotropic phenomenon for dynamic control

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Chinese Journal of Chemical Engineering 2019年第7期27卷 1510-1522页

作者： Yixin Ma peizhe Cui Yongkun wang Zhaoyou Zhu Yinglong wang Jun Gao College of Chemical and Environmental Engineering Shandong University of Science and TechnologyQingdao 266590China College of Chemical Engineering Qingdao University of Science and TechnologyQingdao 266042China

Extractive distillation is an effective method for separating azeotropic or close boiling point mixtures by adding a third *** technologies for performing the extractive distillation process have been explored to protect the environment and save *** paper focuses on the improvement of these advanced technologies in recent *** distillation is retrieved and analyzed from the view of phase equilibrium,selection of solvent in extractive distillation,process design,energy conservation,and dynamic *** quantitative structure–property relationship used in extractive distillation is discussed,and the future development of extractive distillation is proposed to determine how the solvent affects the relative volatility of the separated *** the steady state design,the relationship between the curvature of the residue curve and parameters of the optimal steady state is also highlighted as another field worthy of further study to simplify the distillation process.

关键词： Thermodynamic Quantitative Structure-property relationship Solvent selection Process design Energy conservation Dynamic control

Determination of a suitable index for a solvent via two-column extractive distillation using a heuristic method

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Frontiers of Chemical Science and Engineering 2020年第5期14卷 824-833,F0004页

作者： Zhaoyou Zhu Guoxuan Li Yao Dai peizhe Cui Dongmei Xu Yinglong wang College of Chemical Engineering Qingdao University of Science and TechnologyQingdao 266042China College of Chemical and Environmental Engineering Shandong University of Science and TechnologyQingdao 266590China Shandong Collaborative Innovation Center of Eco-Chemical Engineering Qingdao University of Science and TechnologyQingdao 266042China

The traditional approach to solvent selction in the extractive distillation process strictly focuses on the change in the relative voltility of light-heavy components induced by the ***,the total annual cost of the process may not be minimal when the solvent induces the largest change in relative *** work presents a heuristic method for selecting the optimal solvent to minimize the total annual *** functional relationship between the relative volatility and the total annual cost is established,where the main factors,such as the relative volatility of the light-heavy components and the relative volatility of the heavy-component solvent,are taken into *** azeotropic mixtures of methanol-toluene and methanol-acetone are separated to verify the feasibility of the *** results show that using the solvent with the minimal two-column extractive distillation index,the process achieves a minimal total annual *** method is conducive for sustainable advancements in chemistry and engineering because a suitable solvent can be selected without simulation verification.

关键词： heuristic method solvent selection extractive distillation total annual cost

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Chinese Journal of Aeronautics 2023年第5期36卷 18-32页

作者： Zhuming RAO Ruichao LI peizhe ZHAO Bing wang Dan ZHAO Qiaofeng XIE School of Aerospace Engineering Tsinghua UniversityBeijing 100084China Department of Mechanical Engineering University of CanterburyChristchurch 8041New Zealand

In this study,flame responses to acoustic disturbances with different frequencies and amplitudes were experimentally investigated in a lean premixed swirl-stabilized combustor operating at different bulk *** total heat release rate fluctuations and spatial CH*chemiluminescence distributions were captured using a photomultiplier tube and high-speed camera,*** results indicate that the heat release rate exhibits a relatively drastic oscillation and high-order harmonics for low-frequency *** the bulk velocity and forcing frequency were doubled simultaneously,similar flame structures were observed in the CH*chemiluminescence *** the bulk velocity increases,the gain of the Flame Describing Function(FDF)extends toward the higher frequencies,and the delay time of the flame response *** similarity among FDFs at different bulk velocities was effectively captured by introducing a non-dimensional parameter,defined as the ratio of the flame response delay to the forcing time scale,to replace the dimensional forcing ***,the availability of the newly defined non-dimensional parameter was verified for flames with different swirl numbers,as this played an important role in determining the flame structures and associated unsteady heat release rate.

关键词： Bulk velocity Combustion instability Flame describing function Strouhal number Swirling flame Swirl number

Elucidation of the last steps of photo-ammonification:analytical method development and mechanism elucidation

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Frontiers of Environmental Science & Engineering 2024年第8期18卷 101-106页

作者： Yingjing Miao Hui wang Chunpeng Yang Ruochun Zhang Hao Zhang peizhe Sun School of Environmental Science and Engineering Tianjin UniversityTianjin 300072China SINOPEC Research Institute of Petroleum Processing Co. Ltd.Beijing 100083China School of Earth System Science Tianjin UniversityTianjin 300072China

The mechanisms underlying the photo-ammonification of nitrogenous organic compounds(NOCs)remain unclear,partly due to the analytical challenges of small NOC *** study introduced a simple methodology for accurately and simultaneously quantifying multiple small NOCs during ammonification *** developed method employed phenyl isothiocyanate as derivatization reagents,followed by high-performance liquid chromatography analysis to measure primary and secondary amines,amides,as well as NH4+over variable photo-ammonification *** our experimental setup,vacuum ultraviolet(VUV)irradiation serves as the controlled reaction environment to simulate harsh photo-ammonification *** NOCs,including pyridine,N,N-dimethylformamide,and acrylonitrile,were chosen due to their structural diversity and environmental relevance as model *** method was able to achieve excellent nitrogen mass balance,and revealed that the last steps of photo-ammonification involved oxidation of nitrogen-adjacent carbon to amide followed by the cleavage of N-C *** novel method may also help quantitative investigation of nitrogen transformations in different environmental contexts.

关键词： Ammonification NOCs Amines Nitrogen mass balance

A New Method for Fitting Current–Voltage Curves of Planar Heterojunction Perovskite Solar Cells

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Nano-Micro Letters 2018年第1期10卷 45-52页

作者： peizhe Liao Xiaojuan Zhao Guolong Li Yan Shen Mingkui wang Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Ningxia University

Herein we propose a new equivalent circuit including double heterojunctions in series to simulate the current–voltage characteristic of P–I–N planar structure perovskite solar cells. This new method can theoretically solve the dilemma of the parameter diode ideal factor being larger than2 from an ideal single heterojunction equivalent circuit,which usually is in the range from 1 to 2. The diode ideal factor reflects PN junction quality, which influences the recombination at electron transport layer/perovskite and perovskite/hole transport layer interface. Based on the double PN junction equivalent circuit, we can also simulate the dark current–voltage curve for analyzing recombination current(Shockley–Read–Hall recombination) and diffusion current(including direct recombination), and thus carrier recombination and transportation characteristics. This new model offers an efficacious and simple method to investigate interfaces condition, film quality of perovskite absorbing layer and performance of transport layer, helping us furtherimprove the device efficiency and analyze the working mechanism.

关键词： Dark current Device simulation Junction property Perovskite Solar cell