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Overexpression of tonoplast ca^(2+)-ATPase in guard cells synergistically enhances stomatal opening and drought tolerance

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Journal of Integrative Plant Biology 2024年第8期66卷 1587-1602页

作者： Jinghan Su Bingqing He peiyuan Li Baiyang Yu Qiwen cen Lingfeng Xia Yi Jing Feibo Wu Rucha Karnik Dawei Xue Michael R.Blatt Yizhou Wang Institute of crop Science Zhejiang UniversityHangzhou 310058China Laboratory of Plant Physiology and Biophysics University of GlasgowGlasgow G128QQUK BGI Research Sanya 572025China college of Life and Environmental Sciences Hangzhou Normal UniversityHangzhou 311121China Zhejiang Provincial Key Laboratory of crop Germplasm Zhejiang UniversityHangzhou 310058China

Stomata play a c.ucial role in plants by controlling water status and responding to drought ***,simultaneously improving stomatal opening and drought tolerance has proven to be a significant *** address this issue,we employed the OnGuard quantitative model,which ac.urately represents the mechanics and coordination of ion transporters in guard *** the guidance of OnGuard,we successfully engineered plants that overexpressed the main tonoplast ca^(2+)-ATPase gene,AcA11,which promotes stomatal opening and enhances plant ***,these transgenic plants also exhibited improved drought tolerance due to reduced water loss through their ***,OnGuard assisted us in understanding the mechanism behind the unexpected stomatal behaviors observed in the AcA11 overexpressing *** study revealed that the overexpression of AcA11 facilitated the ac.umulation of ca^(2+)in the vacuole,thereby influencing ca^(2+)storage and leading to an enhanced ca^(2+)elevation in response to abscisic *** regulatory cascade finely tunes stomatal responses,ultimately leading to enhanced drought *** findings underscore the importance of tonoplast ca^(2+)-ATPase in manipulating stomatal behavior and improving drought ***,these results highlight the diverse functions of tonoplast-localized AcA11 in response to different conditions,emphasizing its potential for future applications in plant enhancement.

关键词： AcA11 drought guard cells modeling stomata

Polio eradication surveillance in Sri Lanka,2019-2023

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Asian Pacific Journal of Tropical Medicine 2024年第6期17卷 268-272页

作者： M.A.Y.Fernando N.S.Madarasinghe c.Rangana N.Weerasinghe D.c.u.D.Weligamage J.I.Abeynayake Department of Medical Microbiology and Immunology Faculty of MedicineUniversity of ColomboSri Lanka Department of Virology Medical Research InstituteColombo 08Sri Lanka Medical Research Institute Colombo 08Sri Lanka

Objective:To evaluate the polio laboratory surveillance carried out from January,2019 to May,2023 by the Polio Regional Reference Laboratory,Sri ***:This retrospective study analyzed all stool samples received under the acute flaccid paralysis(AFP)and immunodeficient vaccine-derived poliovirus(VDPV)surveillance at Polio Regional Reference Laboratory,Sri Lanka from January,2019 to May,*** results of the testing methodologies were extracted from the laboratory data system,i.e.,poliovirus virus isolation,intra-typic differentiation/VDPV real time reverse transcriptase polymerase chain reaction(ITD/VDPV rRTPcR)and sequencing,along with the data on timing of reporting results,stool adequacy and *** was analyzed using descriptive ***:A total of 2141 stool samples from 1644 cases were received for AFP surveillance from Sri Lanka(93.61%),Maldives(1.52%),and immunodeficient VDPV(4.86%)*** polioviruses(19/1644,1.15%)and non-polio enteroviruses(73/1644,4.44%)were isolated,while Sabin-like 3 virus was detected in majority(12/19,63.15%)among the poliovirus *** polioviruses or circulating VDPVs were not detected among the *** all years of the study,the non-polio AFP detection rate was>1/100000 in children aged less than 15 years,whereas stool adequacy rate was>80%.All results were reported within 14 days of receipt,ensuring timely reporting as per global ***:The Polio Regional Reference Laboratory,Sri Lanka plays a vital role in maintaining the polio-free status in the c.untry through its robust laboratory surveillance,while adhering to the surveillance ***-detection of wild polioviruses and circulating VDPV during the study period reinforces the polio-free status in the c.untry.

关键词： Poliovirus Polio laboratory surveillance Regional reference laboratory Polio eradication

Advancing public health preparedness:Establishment of Nipah virus molecular diagnostic test at the National Reference Laboratory,Sri Lanka

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Asian Pacific Journal of Tropical Medicine 2024年第8期17卷 369-374页

作者： Hewa Babarandage chathurika Harshani Denagama Vitharanage Rishan Geeth Ruwan udage Kankanamge Isuru udara Samaraweera Dedunu c.u Dias Weligamage Janaki I Abeynayake Medical Research Institute Colombo-08Sri Lanka

Objective:To establish Nipah virus diagnostic capabilities at the National Reference Laboratory in Sri Lanka using the NIV Pune real-time PcR ***:Strict safety prec.utions were adhered during testing due to the high pathogenicity of the Nipah virus,with all diagnostics conducted in a BSL2+laboratory at the Medical Research Institute in Sri *** extraction was performed using the QIAamp Viral RNA Mini *** NIV Pune in-house real-time PcR kit was employed,following established primer/probe sequences and *** assay was validated using the Rotor-Gene Q Series Real-time PcR ***:The validation run of the Nipah virus real-time PcR test demonstrated robust performance,with positive controls consistently detecting Nipah RNA at a ct value of 21.50±*** controls confirmed assay specificity with an external negative control which was also used as an extraction control and showed no *** internal control exhibited stable behavior,enhancing confidence in PcR *** qPcR analysis graph illustrated the successful detection of internal and positive controls,validating the reliability of the ***:Establishing Nipah virus diagnostic capabilities in Sri Lanka signifies a proactive and collaborative response to the persistent global health threat.

关键词： Nipah virus Real-time PcR Validation Molecular diagnostics

Muon energy reconstruction in the high-energy neutrino observations

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Astronomical Techniques and Instruments 2024年第3期1卷 197-202页

作者： Ying Qi Jiali Liu Maoyuan Liu Mingjun chen Zike Wang Tianqi Huang peiyuan c.u college of Science Tibet UniversityLhasa 850000China Key Laboratory of cosmic Rays Ministry of EducationTibet UniversityLhasa 850000China Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China Tianfu cosmic Ray Research center Chengdu 610000China university of chinese Academy of Sciences Beijing 100049China

The reconstruction of muon energies is c.ucial for the data analysis of neutrino experiments using large water cherenkov detectors,but the resolution for muon energy reconstruction using traditional methods is ***,we propose a revised approach to remove noisy optical modules along the track produced by the propagation of muons through *** number of photons on the optical modules is first corrected by the attenuation properties of light in *** the difference in time between the observed optical modules and the expected ones is determined based on the geometry of the triggered optical ***,the standard of correction is measured by the ratio of photon number before and after *** modules selection conditions were optimized according to these parameters,with most noisy optical modules successfully removed,improving the resolution of muon energy reconstruction.

关键词： dE/dx detectors Neutrino detectors Data processing

Role of marine natural products in the development of antiviral agents against SARS‑coV‑2:potential and prospects

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Marine Life Science & Technology 2024年第2期6卷 280-297页

作者： D.P.Nagahawatta N.M.Liyanage Thilina u.Jayawardena H.H.A.c.K.Jayawardhana Seong‑Hun Jeong Hyung‑Jun Kwon You‑Jin Jeon Department of Marine Life Sciences Jeju National UniversityJeju 690-756Republic of Korea Department of chemistry Biochemistry and PhysicsUniversitédu QuébecàTrois-RivièresTrois-RivièresQC G8Z 4M3Canada Marine Science Institute Jeju National UniversityJeju 63333Republic of Korea Functional Biomaterial Research center Korea Research Institute of Bioscience and BiotechnologyJeongeup-siRepublic of Korea

A novel coronavirus,known as severe acute respiratory syndrome coronavirus 2(SARS-coV-2),has surfaced and c.used global concern owing to its ***-coV-2 is the c.usative agent of coronavirus disease 2019;however,it was only discovered at the end of the year and was considered a pandemic by the World Health ***,the develop-ment of novel potent inhibitors against SARS-coV-2 and future outbreaks is urgently *** naturally oc.urring bioactive substances have been studied in the clinical setting for diverse *** intricate infection and replication mechanism of SARS-coV-2 offers diverse therapeutic drug targets for developing antiviral medicines by employing natural products that are safer than synthetic *** natural products(MNPs)have received increased attention in the development of novel drugs owing to their high diversity and ***,this review article investigates the infection and replication mechanisms,inc.uding the function of the SARS-coV-2 genome and ***,we highlighted anti-SARS-coV-2 therapeutic intervention efforts utilizing MNPs and predicted SARS-coV-2 inhibitor design.

关键词： SARS-coV-2 Marine natural products Drug targets Inhibitors

Old-growth mixed dipterocarp forests show variable losses and gains in aboveground biomass and standing carbon over forty years

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Forest Ecosystems 2024年第1期11卷 14-23页

作者： Sisira Ediriweera champika Bandara Tithira Lakkana Shanika Jayasinghe David Woodbury Arun Dayanandan B.M.P.Singhakumara Xiangcheng Mi I.A.u.N.Gunatilleke c.V.S.Gunatilleke Mark SAshton Department of Science and Technology Uva Wellassa UniversityBadullaSri Lanka The Forest School Yale School of the EnvironmentYale UniversityNew HavenCT06511USA Department of Forestry and Environmental Science University of Sri JayewardenepuraNugegodaSri Lanka State Key Laboratory of Vegetation and Environmental change Institute of BotanyChinese Academy of SciencesBeijing100093China Botany Department University of PeradeniyaPeradeniyaSri Lanka

Background:No studies have documented long-term trends in aboveground biomass(AGB)for mixed-dipterocarp forests(MDF),the dominant rain forest type in tropical wet equatorial *** our study,we sought to document such trends over forty years across three sites representing lowland to lower montane ***:To do this,we established fifty 100 m×25 m plots in 1978 across three sites sampled along an elevation gradient,identified as mature old-growth *** measured trees for diameter at breast height that we identified to species and *** took wood samples to calculate species wood-specific *** re-measured plots in 1998 and again in ***:We show standing AGB for all sites combined to be 517.52 Mg·ha^(-1)in 1978,but this declined by 17%over 40 years to 430.11 Mg·ha^(-1).No differences exist among sites in AGB primarily bec.use of considerable within site variation;but interactions of time with site show declines across sites were not uniform,one remained about the *** few species represented a high proportion of the AGB with the top five species comprising between 34%and 65%,depending upon site and year *** species,Mesua nagassarium,represented a disproportionately large amount of AGB and decline over time,particularly at the low elevation ***:Our results are directly relevant to estimating AGB and standing carbon sequestered in *** study is the first to demonstrate varying but overall,declining trends in amounts of AGB among forests making predictions of biomass and standing carbon in MDF difficult over wide regions.

关键词： Western ghats Shorea spp. Southeast Asia Sri Lanka Tropical rain forest Wood specific gravity

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多系统单频紧组合GNSS-R测高方法

武汉大学学报（信息科学版） 2024年第1期49卷 146-155页

作者：邓垦周佩元杜兰蔡巍信息工程大学地理空间信息学院河南郑州450001 77120部队四川成都610000 中国科学院精密测量科学与技术创新研究院大地测量与地球动力学国家重点实验室湖北武汉430077 西安测绘总站陕西西安710054

全球卫星导航系统反射测量(global navigation satellite system-reflectometry,GNSS-R)技术可以同时接收导航卫星直射信号和来自水面的反射信号,进而利用伪距、载波相位和信噪比信息实现高时空分辨率的水面高度反演。由于岸基设备特别... 详细信息

全球卫星导航系统反射测量(global navigation satellite system-reflectometry,GNSS-R)技术可以同时接收导航卫星直射信号和来自水面的反射信号,进而利用伪距、载波相位和信噪比信息实现高时空分辨率的水面高度反演。由于岸基设备特别是单频设备在部分受限环境中观测量不足,导致基于载波相位处理的GNSS-R实时测高性能降低,因此提出了一种利用GNSS重叠频率B1c/L1/E1信号的紧组合模型以提升单频GNSS-R的测高性能。首先,从天线极化方式的角度对差分系统间偏差(differential inter-system bias,DISB)进行评估和分析。结果表明,对于左圆极化天线和右圆极化天线组成的短基线,在使用同型号接收机时可以忽略DISB的影响。然后,基于实测北斗三号卫星导航系统/全球定位系统(global positioning system,GPS)/伽利略(Galileo)系统数据评估了多系统紧组合模型下的GNSS-R测高性能。实验结果表明,相比于松组合模型,多系统紧组合模型能够显著减少安装环境对设备的限制,特别是在单系统反射信号观测量不足4个的条件下,紧组合模型GNSS-R水面高度反演在保证厘米级精度的条件下成功率仍高达约94%,从而有效提升恶劣观测条件下GNSS-R测高性能。

关键词： GNSS反射测量差分系统间偏差反射信号多系统紧组合

Generating Baikal-GVD high energy cascade-like neutrino events with a GEANT4-based simulation toolkit

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chinese Physics c 2024年第10期48卷 256-263页

作者： Zike Wang A.D.Avrorin Zhen cao Mingjun chen peiyuan c.u Zh.-A.M.Dzhilkibaev Bo Gao Tianqi Huang Jiali Liu Ying Qi Xiaohao You D.N.Zaborov Key Laboratory of Particle Astrophysics Experimental Physics Division&Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China university of chinese Academy of Sciences Beijing 100049China Institute for Nuclear Research of the Russian Academy of Sciences Moscow 117312Russia TIANFu cosmic Ray Research center Chengdu 610213China School of Science Tibet UniversityLhasa 850000China

using the GEANT4 and cosmic Ray Monte carlo(cRMc)software packages,we developed a new simulation toolkit for astrophysical neutrino *** configuring the Baikal-GVD detector and comparing the vertex position and direction of incident particles,as well as the channel-by-channel signals,to the events detected by Baikal-GVD,we successfully generated 13 high-energy cascade neutrino events with the *** analysis revealed a systematic offset between the reconstructed shower position and the true interaction position,with a distance close to the scale of the shower maximum of−0.54±1.29 *** achieved a good linear relationship between the photoelectron number of neutrino events obtained by simulation and the real data measured by *** simulation toolkit c.uld serve as a reliable basis for studying the performance of astrophysical neutrino telescopes.

关键词： high-energy neutrino cascade event simulation cherenkov

Generation of Eco-Friendly and Disease-Resistant channel catfish(Ictalurus punctatus)Harboring the Alligator cathelicidin Gene via cRISPR/cas9 Engineering

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Engineering 2024年第8期39卷 273-286页

作者： Jinhai Wang Baofeng Su De Xing Timothy J.Bruce Shangjia Li Logan Bern Mei Shang Andrew Johnson Rhoda Mae c.Simora Michael coogan Darshika u.Hettiarachchi Wenwen Wang Tasnuba Hasin Jacob Al-Armanazi cuiyu Lu Rex A.Dunham School of Fisheries Aquacultureand Aquatic SciencesAuburn UniversityAuburnAL 36849USA

As a precise and versatile tool for genome manipulation,the c.ustered regularly interspaced short palindromic repeats(cRISPR)/cRISPR-associated protein 9(cas9)platform holds promise for modifying fish traits of *** the aim of reducing transgene introgression and controlling reproduction,upscaled disease resistance and reproductive intervention in catfish species have been studied to lower the potential environmental risks of the introgression of escapees as transgenic *** advantage of the cRISPR/cas9-mediated system,we succeeded in integrating the cathelicidin gene(As-cath)from an alligator(Alligator sinensis)into the target luteinizing hormone(lh)locus of channel catfish(Ictalurus punctatus)using two delivery systems assisted by double-stranded DNA(dsDNA)and single-stranded oligodeoxynucleotides(ssODNs),*** this study,high knock in(KI)efficiency(22.38%,64/286)but low ontarget events was achieved using the ssODN strategy,whereas adopting a dsDNA as the donor template led to an efficient on-target KI(10.80%,23/213).The on-target KI of As-cath was instrumental in establishing the lh knockout(LH^(–)_As-cath^(+))catfish line,which displayed heightened disease resistance and reduced fecundity compared with the wild-type(WT)sibling ***,administration of human chorionic gonadotropin(HcG)and luteinizing hormone-releasing hormone analogue(LHRHa)can restore the reproduction of the transgenic fish ***,we replaced the lh gene with an alligator cathelicidin transgene and then administered hormone therapy to move towards complete reproductive control of diseaseresistant transgenic catfish in an environmentally responsible *** strategy not only effectively improves consumer-valued traits but also guards against unwanted introgression,providing a breakthrough in aquaculture genetics to confine fish reproduction and prevent the establishment of transgenic or domestic genotypes in the natural environment.

关键词： Genome editing ssODN dsDNA Antimicrobial peptide Reproductive confinement Aquaculture