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Super-resolution quantification of nanoscale damage to mitochondria in live cells

Nano Research 2020年第8期13卷 2149-2155页

作者： Xintian Shao Qixin Chen Lianting Hu Zhiqi Tian liuyi liu Fei liu Fengshan Wang peixue Ling Zong-Wan Mao Jiajie Diao Shandong Academy of Pharmaceutical Sciences Key Laboratory of BiopharmaceuticalsEngineering Laboratory of Polysaccharide DrugsNational-Local Joint Engineering Laboratory of Polysaccharide DrugsJinan250101China Department of Cancer Biology University of Cincinnati College of MedicineCincinnatiOH45267USA School of Pharmaceutical Sciences Cheeloo College of MedicineShandong UniversityJinan250012China Xintian Shao Qixin Chenand Lianting Hu contributed equally to this work.Shandong First Medical University&Shandong Academy of Medical SciencesJinan250062China Xintian Shao Qixin Chenand Lianting Hu contributed equally to this work.School of Information ManagementWuhan UniversityWuhan430072China MOE Key Laboratory of Bioinorganic and Synthetic Chemistry School of ChemistrySun Yat-Sen UniversityGuangzhou510275China

Mitochondrial damage,characterized by altered morphological distribution and the damage of cristae,is closely associated with mitochondrial ***,imaging methods for capturing mitochondrial morphology at the nanoscale level in live samples remain unavailable,which seriously hinders the accurate evaluation and diagnosis of mitochondrial-related *** response,we propose a super-resolution quantification strategy based on structured illumination microscopy(SIM)for the rapid,accurate evaluation of mitochondrial *** the strategy,we accurately captured the morphological distribution of mitochondria at the nanoscale level in a way generally applicable to checking various cell processes and identifying patients with mitochondrial disease who exhibit the SLC25A46 *** also used algorithm-assisted super-resolution imaging to quantitatively analyze damage to mitochondrial cristae,which supports a novel drug screening strategy—high-resolution drug screening—for investigating drugs’pharmacodynamics on organelles in living *** short,our strategy improves the accurate examination of changes in mitochondrial morphology in living cells and indicates new ways in which SIM-imaging can assist in diagnosing mitochondrial disease at the single-cell level.

关键词： mitochondria structured illumination microscopy quantification analyze morphology cristae

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中国旅游地理研究:成果应用转化和研究技术革新

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中国生态旅游 2021年第1期11卷 52-65页

作者：吴必虎黄潇婷刘培学黄震方张捷黄珊蕙孙晋坤北京大学城市与环境学院旅游研究与规划中心北京100871 山东大学管理学院济南250100 山东大学旅游行为实验室济南250100 南京师范大学地理科学学院南京210023 南京大学地理与海洋科学学院南京210093

旅游的产业属性决定了旅游地理研究的实践性,在服务国家战略和产业实践需求的过程中,中国旅游地理学科呈现出研究领域、方法和应用的新特征。本文围绕旅游规划、旅游资源、旅游大数据和旅游实验方法等进行总结与展望,认为:(1)旅游规划... 详细信息

旅游的产业属性决定了旅游地理研究的实践性,在服务国家战略和产业实践需求的过程中,中国旅游地理学科呈现出研究领域、方法和应用的新特征。本文围绕旅游规划、旅游资源、旅游大数据和旅游实验方法等进行总结与展望,认为:(1)旅游规划是透视旅游地理研究发展的重要视角,旅游规划的知识域主要包括旅游开发战略与对策、旅游业与旅游企业、乡村旅游与乡村振兴等12个方面;(2)新时代文化和旅游资源普查面临文旅资源保护和整合利用的重大现实需求,为旅游地理研究带来了文旅资源的内涵与分类评价、普查信息集成与应用、普查技术与组织方式等新命题;(3)大数据背景下要求旅游地理探寻新科学问题,重点要关注大数据旅游统计测量模型和指标规则的规范,数据伦理与信息茧房,大数据旅游现象的地理效应和相关空间格局等研究;(4)实验研究方法在认识论层面从描述走向解释预测,在方法论层面从调查走向直接测量,为认识和理解旅游情境下人地关系的核心科学问题提供了"科学化"的研究路径。因此,面对新时代战略要求和旅游业发展趋势,旅游地理学应加强产业实践、研究方法和学科交叉研究,提升学科服务国家战略和社会经济发展的支撑能力。

关键词：旅游地理学旅游规划知识域旅游资源普查旅游大数据旅游实验方法

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Research Progress of Sunscreen Agents and Encapsulate Technology

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China Detergent & Cosmetics 2023年第2期8卷 56-61页

作者： Zheng Xiaoyang Meng Xiangjing Li Min liu Bo Zhang Xiangkui Duan Chong gang Zhang Daizhou Ling peixue Wang Dongliang Shandong Engineering Research Center of Skin and Mucosa Drug Administration Shandong Academy of Pharmaceutical SciencesChina Hebei Edible Bird’s Nest Fresh Stew Technology Innovation Center China

The harm of ultraviolet radiation to human skin and the mechanism of traditional sunscreen agents and natural sunscreen agents were *** agents were usually encapsulated with delivery systems due to the instability and skin *** research progress of several common sunscreen delivery systems was introduced,the future research direction of sunscreen agents was also prospected.

关键词： UV radiation sunscreen encapsulate photo-protection

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Drosophila Homolog of FMRP Maintains Genome Integrity by Interacting with Piwi

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Journal of Genetics and Genomics 2016年第1期43卷 11-24页

作者： Fangfang Jiang Falong Lu peixue Li Wei liu Lu Zhao Qifu Wang Xiaofeng Cao Lei Zhang Yong Q.Zhang State Key Laboratory for Molecular and Developmental Biology Institute of Genetics and Developmental Biology Chinese Academy of Sciences State Key Laboratory of Plant Genomics and National Center for Plant Gene Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Science and Technology Center Fenyang College Shanxi Medical University Department of Medical Laboratory Science Fenyang College Shanxi Medical University

Fragile X syndrome （FraX）, the most common form of inherited mental retardation, is caused by the absence of the evolutionally conserved fragile X mental retardation protein （FMRP）. While neuronal functions of FMRP have been intensively studied for the last two decades, its role in non-neuronal cells remains poorly understood. Piwi, a key component of the Piwi-interacting RNA （piRNA） pathway, plays an essential role in germline development. In the present study, we report that similar to piwi, dfmrl, the Drosophila homolog of human FMR1, is required for transposon suppression in the germlines. Genetic analyses showed that dfmrl and piwi act synergistically in heterochromatic silencing, and in inhibiting the differentiation of primordial germline cells and transposon expression. Northern analyses showed that roo piRNA expression levels are reduced in dfmrl mutant ovaries, suggesting a role of dfmrl in piRNA biogenesis. Biochemical analysis demonstrated a physical interaction between dFMRP and Piwi via their N-termini. Taken together, we propose that dFMRP cooperates with Piwi in maintaining genome integrity by regulating heterochromatic silencing in somatic cells and suppressing transposon activity via the piRNA pathway in germlines.

关键词： Fragile X syndrome FMRP piwi piRNA Germline

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Nanoscale monitoring of mitochondria and lysosome interactions for drug screening and discover

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Nano Research 2019年第5期12卷 1009-1015页

作者： Qixin Chen Xintian Shao Zhiqi Tian Yang Chen Payel Mondal Fei liu Fengshan Wang peixue Ling Weijiang He Kai Zhang Zijian Guo Jiajie Diao School of Pharmaceutical Sciences Shandong UniversityJinan 250101China Shandong Academy of Pharmaceutical Science Key Laboratory of BiopharmaceuticalsEngineering Laboratory of Polysaccharide DrugsNational-Local Joint Engineering Laboratory of Polysaccharide DrugsJinan 250101China Department of Cancer Biology University of Cincinnati College of MedicineCincinnatiOH 45267USA Department of Biochemistry University of Illinois at Urbana-ChampaignUrbanaIL 61801USA State Key Laboratory of Coordination Chemistry Coordination Chemistry InstituteSchool of Chemistry and Chemical EngineeringNanjing University Nanjing 210093China

Technology advances in genomics,proteomics,and metabolomics largely expanded the pool of potential therapeutic *** with the in vitro setting,cell-based screening assays have been playing a key role in the processes of drug discovery and *** the commonly used strategies based on colorimetric and cell viability,we reason that methods that capture the dynamic cellular events will facilitate optimal hit identification with high sensitivity and ***,we propose a live-cell screening strategy using structured illumination microscopy (SIM) combined with an automated cell colocalization analysis software,CellprofilerTM,to screen and discover drugs for mitochondria and lysosomes interaction at a nanoscale resolution in living *** strategy quantitatively benchmarks the mitochondria-lysosome interactions such as mitochondria and lysosomes contact (MLC) and *** automatic quantitative analysis also resolves fine changes of the mitochondria-lysosome interaction in response to genetic and pharmacological ***-resolution live-cell imaging on the basis of quantitative analysis opens up new avenues for drug screening and development by targeting dynamic organelle interactions at the nanoscale resolution,which could facilitate optimal hit identification and potentially shorten the cycle of drug discovery.

关键词： drug screening mitochondria lysosome mitophagy structured illumination microscopy

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SHANK2 is a frequently amplified oncogene with evolutionarily conserved roles in regulating Hippo signaling

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Protein & Cell 2021年第3期12卷 174-193页

作者： Liang Xu peixue Li Xue Hao Yi Lu Mingxian liu Wenqian Song Lin Shan Jiao Yu Hongyu Ding Shishuang Chen Ailing Yang Yi Arial Zeng Lei Zhang Hai Jiang State Key Laboratory of Cell Biology CAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai 200031China School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China Bio-Research Innovation Center Shanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesSuzhou 215121China

Dysfunction of the Hippo pathway enables cells to evade contact inhibition and provides advantages for cancerous ***,for a significant portion of human cancer,how Hippo signaling is perturbed remains *** answer this question,we performed a genome-wide screening for genes that affect the Hippo pathway in Drosophila and cross-referenced the hit genes with human cancer *** our screen,Prosap was identified as a novel regulator of the Hippo pathway that potently affects tissue ***,a mammalian homolog of Prosap,SHANK2,is the most frequently amplified gene on 11 q13,a major tumor amplicon in human *** amplification profile in this 11q13 amplicon clearly indicates selective pressure for SHANK2 *** importantly,across the human cancer genome,SHANK2 is the most frequently amplified gene that is not located within the Myc *** studies in multiple human cell lines confirmed that SHANK2 overexpression causes deregulation of Hippo signaling through competitive binding for a LATS1 activator,and as a potential oncogene,SHANK2 promotes cellular transformation and tumor formation in *** cancer cell lines with deregulated Hippo pathway,depletion of SHANK2 restores Hippo signaling and ceases cellular *** together,these results suggest that SHANK2 is an evolutionarily conserved Hippo pathway regulator,commonly amplified in human cancer and potently promotes *** study for the first time illustrated oncogenic function of SHANK2,one of the most frequently amplified gene in human ***,given that in normal adult tissues,SHANK2 s expression is largely restricted to the nervous system,SHANK2 may represent an interesting target for anticancer therapy.

关键词： SHANK2 oncogene Hippo signaling cancer

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水热炭对餐厨垃圾厌氧消化性能影响研究

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可再生能源 2023年第10期41卷 1287-1293页

作者：刘永侠宋培学唐响超徐杰枣庄学院城市与建筑工程学院山东枣庄277160 北京市化工职业病防治院北京100089

文章通过餐厨垃圾的批式厌氧消化试验研究了水热炭制备温度和水热炭含量对不同接种比下餐厨垃圾产沼气的影响。研究结果表明:未添加水热炭时,随着接种比由1.5降低到0.5,产气抑制严重,餐厨垃圾的产沼气潜力由383.4 mL/g降低到151.6 mL/g... 详细信息

文章通过餐厨垃圾的批式厌氧消化试验研究了水热炭制备温度和水热炭含量对不同接种比下餐厨垃圾产沼气的影响。研究结果表明:未添加水热炭时,随着接种比由1.5降低到0.5,产气抑制严重,餐厨垃圾的产沼气潜力由383.4 mL/g降低到151.6 mL/g;添加水热炭能够提高产气量,减少产气延滞时间;接种比相同时,沼气总产量随着水热炭添加量的增加而提高;当水热炭(H240)的添加量为30%时,接种比为1.5,1和0.5的试验组的沼气总产量较对照组的最大增加量分别为11.5%,31.7%和81.2%;当水热炭添加量相同时,H240对厌氧消化的促进效果最优,这归功于其表面丰富和活跃的官能团;添加水热炭后,修正Gompertz方程对不同接种比下累积产气量的拟合效果均得到了提高。

关键词：餐厨垃圾接种比产气动力学表面官能团水热炭含量

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纳米多孔氩薄膜热导率的分子动力学模拟

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计算物理 2008年第4期25卷 457-462页

作者：刘其鑫姜培学向恒热科学与动力工程教育部重点实验室清华大学热能工程系北京100084

利用经典的Lennard-Jones势能模型,采用分子动力学模拟方法研究纳米多孔氩薄膜热导率.结果表明,纳米多孔氩薄膜热导率明显小于同温度下纯纳米氩薄膜热导率,并且孔隙率越大热导率减小越多.借助基于并联方法的有效热导率模型,对纳米多孔... 详细信息

利用经典的Lennard-Jones势能模型,采用分子动力学模拟方法研究纳米多孔氩薄膜热导率.结果表明,纳米多孔氩薄膜热导率明显小于同温度下纯纳米氩薄膜热导率,并且孔隙率越大热导率减小越多.借助基于并联方法的有效热导率模型,对纳米多孔氩薄膜的热导率随孔隙率的变化进行解释.模拟结果还发现,薄膜内的多孔分布对薄膜的热导率有影响.

关键词：热导率多孔纳米氩薄膜

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急诊胃镜对经皮冠状动脉介入术后合并上消化道出血的诊治价值

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中华消化内镜杂志 2022年第2期39卷 146-148页

作者：王培学董卫国柳舟曹明为谭小平武汉大学人民医院消化内科武汉430060 武汉大学人民医院重症监护室武汉430060 武汉大学人民医院儿科武汉430060 长江大学附属第一医院荆州市第一人民医院消化内科荆州434000

2015年6月-2020年 6月期间, 因经皮冠状动脉介入术(percutaneous coronary intervention, PCI)术后合并上消化道出血, 在武汉大学人民医院行急诊胃镜检查和治疗的52例患者纳入回顾性分析, 主要观察急诊胃镜出血病因诊断、止血治疗结果... 详细信息

2015年6月-2020年 6月期间, 因经皮冠状动脉介入术(percutaneous coronary intervention, PCI)术后合并上消化道出血, 在武汉大学人民医院行急诊胃镜检查和治疗的52例患者纳入回顾性分析, 主要观察急诊胃镜出血病因诊断、止血治疗结果和诊疗过程中并发症发生情况。其中, 47 例(90.4%)急诊胃镜下发现可解释上消化道出血的病灶, 其余5例(9.6%)未能明确诊断出血病因。26例(50.0%)行急诊内镜下止血治疗, 其中25例(96.2%)急诊内镜下止血成功。急诊内镜诊疗中位用时18 min, 7例(13.5%)出现一过性心率减慢及血压降低, 无严重并发症发生。由此可见, 急诊胃镜在PCI术后合并上消化道出血诊治中安全有效。

关键词：止血,内窥镜急诊胃镜经皮冠状动脉介入术上消化道出血

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纳米氩颗粒导热性能的非平衡分子动力学模拟

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清华大学学报（自然科学版） 2008年第2期48卷 248-251页

作者：向恒姜培学刘其鑫清华大学热能工程系热科学与动力工程教育部重点实验室北京100084

为了研究尺寸变化对纳米颗粒导热的影响，采用非平衡分子动力学模拟方法研究了固定原子壁面边界条件下，平均温度在45K、边长为3．186～12．744nm的立方形和半径为4．248～10．62nm的球形2种纳米Ar颗粒的热导率，并与其他研究者的结果... 详细信息

为了研究尺寸变化对纳米颗粒导热的影响，采用非平衡分子动力学模拟方法研究了固定原子壁面边界条件下，平均温度在45K、边长为3．186～12．744nm的立方形和半径为4．248～10．62nm的球形2种纳米Ar颗粒的热导率，并与其他研究者的结果及薄膜的模拟值进行了对比。模拟结果表明，在固壁边界条件下，2种颗粒的热导率均随着尺寸的增加而趋向块体值，但均小于相同厚度、相同边界条件的Ar薄膜的热导率；球形颗粒的热导率小于边长与其半径相等的立方形颗粒的热导率。

关键词：热导率非平衡分子动力学立方形纳米颗粒球形纳米颗粒

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