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Post-transcriptional regulation of grain weight and shape by the RBP-A-J-K complex in rice

Journal of Integrative Plant Biology 2024年第1期66卷 66-85页

作者： Ding Ren Hui Liu Xuejun Sun Fan Zhang Ling Jiang y.ng Wang ning Jiang peiwen y.n Jinhao Cui Jinshui y.ng Zhikang Li Pingli Lu Xiaojin Luo State Key Laboratory of Genetic Engineering and MOE Engineering Research Center of Gene Technology School of Life SciencesFudan UniversityShanghai 200438China State Key Laboratory of Crop Stress Adaptation and Improvement School of Life SciencesHenan UniversityKaifeng 475004China MOE Key Laboratory of Crop Physiology Ecology and Genetic Breeding College of AgronomyJiangxi Agricultural UniversityNanchang 330045China State Key Laboratory of Crop Gene Resources and Breeding Institute of Crop SciencesChinese Academy of Agricultural Sciences(CAAS)Beijing 100081China College of Agronomy Anhui Agricultural UniversityHefei 230036China

RnA-binding proteins(RBPs)are components of the post-transcriptional regulatory system,but their regulatory effects on complex traits remain *** an integrated strategy involving map-based cloning,functional characterizations,and transcriptomic and population genomic analyses,we revealed that RBP-K(LOC_Os08g23120),RBP-A(LOC_Os11g41890),and RBP-J(LOC_Os10g33230)encode proteins that form an RBP-A-J-K complex that negatively regulates rice yield-related *** of the RBP-A-J-K complex indicated RBP-K functions as a relatively.non-specific RBP chaperone that enables RBP-A and RBP-J to function ***,RBP-J most likely affects GA pathways,resulting in considerable increases in grain and panicle lengths,but decreases in grain width and *** contrast,RBP-A negatively regulates the expression of genes most likely involved in auxin-regulated pathways controlling cell wall elongation and carbohydrate transport,with substantial effects on the rice grain filling process as well as grain length and ***,RBP-K is relatively ancient and highly conserved,whereas RBP-J and RBP-A are more ***,the RBP-A-J-K complex may represent a typical functional model for many RBPs and protein complexes that function at transcriptional and post-transcriptional levels in plants and animals for increased functional consistency,efficiency,and versatility,as well as increased evolutionary *** results clearly demonstrate the importance of RBP-mediated post-transcriptional regulation for the diversity of complex ***,rice grain yield and quality may be enhanced by introducing various complete or partial loss-of-function mutations to specific RBP genes using clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9 technology and by exploiting desirable natural tri-genic allelic combinations at the loci encoding the components of the RBP-A-J-K complex through marker-assisted select

关键词： GL11 grain weight and shape post-transcriptional regulation RnA-binding proteins

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Analysis of the Properties of 44 ABC Transporter Genes from Biocontrol Agent Trichoderma asperellum ACCC30536 and Their Responses to Pathogenic Alternaria alternata Toxin Stress

Analysis of the Properties of 44 ABC Transporter Genes from ...

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中国植物病理学会2024年学术年会

作者： DU Huay.ng ZAHnG yuzhou LIU Kuo GU peiwen CAO Shuang GAO Xiang WAnG Zhiy.ng LIU Zhihua yU Zey.ng School of Agriculture Ningxia University School of Forestry Northeast Forestry University College of Forestry Shenyang Agricultural University

ATP-binding cassette(ABC) transporters are involved in transporting multiple substrates,such as toxins,and may be important for the survival of Trichoderma when encountering biotic *** this study,genome searching revealed that there are 44 ABC transporters encoded in the genome of Trichoderma *** ABC transporters were divided into six types based on three-dimensional(3 D) structure prediction,of which four,represented by 39 ABCs,are involved in transport and the remaining two,represented by 5 ABCs,are involved in regulating *** characteristics of nucleotide-binding domain(nBD) are important in the identification of ABC *** though the 3 D structures of the 79 nBDs in the 44 ABCs are similar,multiple sequence alignment showed they can be divided into three *** total,794 motifs were found in the promoter regions of the 44 ABC genes,of which 541 were cis-regulators related to stress *** characterize how their ABCs respond when *** interact with fungi or plants,*** was cultivated in either minimal media(MM) control,C-hungry.n-hungry,or poplar medium(PdPap) to simulate normal conditions,competition with pathogens,interaction with pathogens,and interaction with plants,*** results show that 17 of 39 transport ABCs are highly expressed in at least one condition,whereas four of the five translation-regulating ABCs are highly expressed in at least one *** these 21 highly expressed ABCs,6 were chosen for RT-qPCR expression under the toxin stress of phytopathogen Alternaria alternata,and the results show ABC01,ABC04,ABC05,and ABC31 were highly expressed and may be involved in pathogen interaction and detoxify.ng toxins from ***.

关键词： biological control ABC protein detoxification

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nonepitaxial growth of a (001) textured L1_0 Fe-Pt film in H_2 and n_2 annealing atmospheres

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Rare Metals 2006年第3期25卷 260-264页

作者： WU peiwen HU Xuerang QIAn Jun yUAn Jun Electron Microscope Laboratory Department of Materials Science and Engineering Tsinghua University Beijing 100084 China

A nonepitaxial （001） textured Fe-Pt alloyed film was obtained by annealing Fe/Pt multilayers in H2. no Such nonepitaxial （001） texture was observed for similar multilayers annealed in n2 atmosphere. Sensitive electron energy loss spectroscopy and the left shift of the L10 FePt （111） diffr cfion peak indicate oxidation in the n2 annealed samples. The oxidation dramatically degrades the （001） texture of the Fe-Pt fdm and induces the composition change in the alloyed layer. The relation between the texture formation and oxidation was discussed.

关键词： metal (001) textured FePt annealing oxidation shift of diffraction peak

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顺酐及其配套正丁烷装置的热联合节能模拟优化

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上海化工 2024年第1期49卷 36-40页

作者：刘万广杨佩雯众一伍德工程有限公司 201100 杭州星传新材料科技有限公司浙江杭州310012

针对顺酐装置及轻烃制丙烷-异丁烷正构装置,在设计过程中进行了热联合系统的优化改造。根据轻烃分离塔(C4组分分离塔)操作温度较低、塔顶-塔底温度梯度小的特点,合理有效地利用顺酐装置副产的大量低温位(85℃)热水,从项目整体设计角度... 详细信息

针对顺酐装置及轻烃制丙烷-异丁烷正构装置,在设计过程中进行了热联合系统的优化改造。根据轻烃分离塔(C4组分分离塔)操作温度较低、塔顶-塔底温度梯度小的特点,合理有效地利用顺酐装置副产的大量低温位(85℃)热水,从项目整体设计角度实现了装置间取冷与供热的互补,实现了1+1>2的效果。项目中E-510和E-202的能耗由优化前的58.61 kg标准油/t产品降低至19.54 kg标准油/t产品,C4分离塔再沸器和顺酐产品冷却器的节能效率达67%。

关键词：热联合精馏塔工艺设计操作参数模拟计算

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木质素衍生介孔碳固体酸制备及催化木聚糖制备糠醛

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精细化工 2024年第10期41卷 2258-2265页

作者：周翠瑶朱沛雯邓晓雅陶昱恒王利群卿青常州大学生物与食品工程学院江苏常州213164

以丙酮/水混合溶剂提取玉米秸秆得到的木质素和间苯三酚为碳前体、乙二醛为交联剂,采用溶剂蒸发诱导自组装法制备了介孔碳(MC),再经磺化合成了木质素衍生介孔碳固体酸(MC-SO_(3)H)。采用SEM、XPS、元素分析仪、n2吸附-脱附和酸度测定对... 详细信息

以丙酮/水混合溶剂提取玉米秸秆得到的木质素和间苯三酚为碳前体、乙二醛为交联剂,采用溶剂蒸发诱导自组装法制备了介孔碳(MC),再经磺化合成了木质素衍生介孔碳固体酸(MC-SO_(3)H)。采用SEM、XPS、元素分析仪、n2吸附-脱附和酸度测定对催化剂进行了表征,以木聚糖转化率和糠醛产率为指标,考察了MC-SO_(3)H催化降解木聚糖制备糠醛的反应条件,探究了MC-SO_(3)H失活的原因。结果表明,MC-SO_(3)H具有120.74 cm^(2)/g的介孔比表面积和4.15 nm的平均孔径;在1.0 g木聚糖、1.0 g MC-SO_(3)H、30 mLγ-戊内酯为溶剂、反应温度180℃、反应时间10 min的最佳反应条件下,木聚糖转化率和糠醛产率分别为90.0%和79.5%。MC-SO_(3)H的总酸度(2.06 mmol/g)和—SO_(3)H酸度(0.82 mmol/g)是其催化活性的主要来源,未反应的木聚糖及反应过程中形成的降解物等在催化剂上附着造成了MC-SO_(3)H的总酸度和—SO_(3)H酸度的下降,是其失活的主要原因。在以木糖代替木聚糖评价MC-SO_(3)H稳定性的实验中,重复使用4次后,木糖转化率和糠醛产率仍可达89.5%和66.8%。

关键词：木质素介孔碳固体酸木聚糖糠醛催化技术

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1型糖尿病患者各发病年龄段胰岛功能短暂缓解期特征及对远期预后的影响

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中国糖尿病杂志 2024年第6期32卷 432-436页

作者：佘雪梅田沛文孙喜明王宁黄正丽乔俊峰郭志芳大同市第五人民医院内分泌科 037009 大同市第五人民医院儿科 037009

目的观察T1DM患者各发病年龄段胰岛功能短暂缓解期特征,探讨其对远期预后的影响。方法选取2014年1月至2018年1月于我院内分泌科治疗的新诊断T1DM患者156例,根据发病年龄分为童年组18例、青少年组108例及成年组30例,比较各组胰岛功能... 详细信息

目的观察T1DM患者各发病年龄段胰岛功能短暂缓解期特征,探讨其对远期预后的影响。方法选取2014年1月至2018年1月于我院内分泌科治疗的新诊断T1DM患者156例,根据发病年龄分为童年组18例、青少年组108例及成年组30例,比较各组胰岛功能短暂缓解情况及随访胰岛功能保留率。结果青少年组BMI、FC-P、2 hC-P高于童年组(P<0.05);成年组蛋白酪氨酸磷酸酶及锌转运体8抗体阳性率低于童年组(P<0.05);青少年组胰岛功能短暂缓解发生率高于童年、成年组(P<0.05);青少年、成年组胰岛功能短暂缓解持续时间低于童年组(P<0.05);青少年组发病1年和4年胰岛功能保留率高于童年、成年组(P<0.05)。广义估计方程分析显示,青少年组远期FC-P、2 hC-P高于童年、成年组(P<0.05);成年组既往短暂缓解患者FC-P、2 hC-P高于童年组(P<0.05)。结论 T1DM青少年患者更易出现胰岛功能短暂缓解,童年患者胰岛功能短暂缓解时间更长;胰岛功能短暂缓解人群中以成年患者远期胰岛功能最佳。

关键词：糖尿病,1型年龄胰岛功能预后

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Porous carbon supported atomic iron as electrocatalysts for acidic oxygen reduction reaction

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Science Bulletin 2018年第4期63卷 213-215页

作者： peiwen yu Renjie Chen Qiao Zhang Sihui Liang Mei ni Wen y.ng Key Laboratory of Cluster Science of Ministry of Education Beijing Key Laboratory of Photoelectronic/Electrophotonic Conversion Materials School of Chemistry and Chemical Engineering Beijing Institute of Technology School of Material Science and Engineering Beijing Institute of Technology Shaanxi Coal and Chemical Industry Technology Development Center Co. LTD State Key Laboratory for Modification of Chemical Fibers and Polymer Materials Donghua University

Iron and nitrogen co-doped carbon(Fe-n-C)materials are promising oxygen reduction reaction(ORR)catalysts due to its considerable catalytic performance[1–4].Currently,the catalytic activity of the Fe-n-C materials still cannot match the performance of commercial Pt/C electrocatalysts due to its low density of active *** dispersion of catalytic active sites is a promising method for improving catalytic efficiency,and making

关键词：反应原子催化剂线多孔铁氧碳催化活动

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伢典凝胶去腐技术对口腔龋病患儿疼痛程度和并发症的影响

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黑龙江医学 2024年第15期48卷 1798-1801页

作者：刘培文吴韫慧樊丽娟郑州市第七人民医院口腔科河南郑州450016

目的:分析伢典凝胶去腐技术对口腔龋病患儿疼痛程度和并发症的影响。方法:选取2022年1—10月郑州市第七人民医院收治的82例口腔龋病患儿作为研究对象,根据治疗方法不同将其分为观察组(n=41)与常规组(n=41)。常规组接受常规机械去腐法,... 详细信息

目的:分析伢典凝胶去腐技术对口腔龋病患儿疼痛程度和并发症的影响。方法:选取2022年1—10月郑州市第七人民医院收治的82例口腔龋病患儿作为研究对象,根据治疗方法不同将其分为观察组(n=41)与常规组(n=41)。常规组接受常规机械去腐法,观察组采用伢典凝胶去腐技术。对比两组患儿疼痛程度、炎症因子白细胞介素-6(IL-6)及肿瘤坏死因子-α(TnF-α)水平及并发症,治疗后3个月、6个月的治疗成功率。结果:治疗后,两组患儿视觉模拟评分法(VAS)评分均降低,且观察组低于常规组,差异有统计学意义(t=36.982,PnF-α水平均升高,但观察组龈沟液中IL-6、TnF-α水平低于常规组,差异有统计学意义(t=13.198、12.571,P<0.05)。观察组并发症总发生率(4.88%)低于常规组(19.51%),差异有统计学意义(t=4.100,P<0.05),治疗后3个月、6个月时,观察组患儿的治疗成功率均高于常规组,差异有统计学意义(t=4.232、4.100,P<0.05)。结论:口腔龋病中应用伢典凝胶去腐技术的效果确切,可改善口腔龋病患儿治疗后疼痛症状,减轻炎症反应,且可降低并发症发生风险。

关键词：口腔龋病伢典凝胶去腐技术并发症白细胞介素-6 肿瘤坏死因子-α

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Effect of Cesium Modification on CuO/CeO2 Catalysts for the Catalytic Decomposition of n2O

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Chemical Research in Chinese Universities 2019年第1期35卷 47-52页

作者： GUO peiwen QIn Feng SUn Chao HUAnG Zhen SHEn Wei XU Hualong Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials Laboratory of Advanced Materials Collaborative Innovation Center of Chemistry for Energy MaterialsDepartment of Chemistry Fudan UniversityShanghai 200433P.R.China

A series of Cs-modified CuO/CeO2 mixed oxide catalysts was prepared for enhancing the stable activity of n2O *** was found that Cs modification promoted the catalytic performance of CuO/CeO2 catalysts significantly,The l%Cs-CuO/CeO2 catalyst exhibited the best activity,and the conversion of n2O reached 100%at 380℃ in the presence of 2% *** catalytic behaviors were investigated by means of XRD,n2 adsorption isotherms,XPS,H2-TPR(TPR:temperature-prograrmmed reduction),CO-IR,O2-TPD(TPD:temperature-programmed desorption)and diffused reflectance infrared Fourier transform spectorscopy(DRiFTs).The results revealed that Cs modification promoted the activity and the oxygen resistance by enhancing the desorption of surface oxygen species and increasing the content of Ce^3+.CO-DRIFTs revealed that Ce^3+could efficiently facilitate the regeneration of active Cu^+sites by an oxygen migration *** possible reaction mechanism was also discussed.

关键词： Cs-Modified CuO/CeO2 n2O decomposition Reaction mechanism

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Spherical Cu2O Assembled by Small nanoparticles and Its High Efficiency in Photodegradations of Methylene Blue Under Different Light Sources

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Chemical Research in Chinese Universities 2020年第6期36卷 1108-1115页

作者： CHEn peiwen ZHU Lianjie CHAnG Zhuangzhuang GAO Hongjia CHEn Deyou QIU Mo School of Chemistry&Chemical Engineering Tianjin Key Laboratory of Organic Solar Cells and Photochemical ConversionTianjin University of TechnologyTianjin 300384P.R.China Agro-Environmental Protection Institute Chinese Academy of Agricultural SciencesTianjin 300191P.R.China

Well-dispersed mesoporous spherical Cu2O assembled by small nanoparticles was synthesized by a green and rapid solution route at room temperature under assistance of ultrasonic,using eco-friendly ascorbic acid(AA)as the main reducing agent and glucose as a weak reducing agent and soft *** CuO spheres were characterized by XRD,SEM,HRTEM,XPS and n2 adsorption-desorption *** optical property was studied by UV-Vis diffuse reflectance absorption spectrum and the photocatalytic activities were evaluted by photodegradations of me thylene blue(MB)under different light *** results show that each spherical Cu2O(ca.200 nm in diameter)was accumulated by.numerous small nanoparticles of 8-10 nm and mesopores exist between the nanoparticles,which result in a high BET surface *** spherical Cu2O exhibits good light-harvesting ability in both UV and visiblelight region and excellent photodegradation activities to high concentrations of MB solutions under both UV-visible light and visible light.

关键词： Spherical Cu2O Photodegradation Methylene blue Visible light

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