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Horticulture Research 2020年第1期7卷 1852-1864页

作者： Youwei Fan Jitao liu Jing Zou Xiangyu Zhang liwei Jiang Kun liu peitao lü Junping Gao Changqing Zhang Department of Ornamental Horticulture College of HorticultureChina Agricultural UniversityBeijing 100193China Crop Research Institute Guangdong Academy of Agricultural SciencesGuangdong Provincial Key Laboratory of Crop Genetic ImprovementGuangzhouGuangdong 510642China

Phytohormones are key factors in plant responsiveness to abiotic and biotic stresses,and maintaining hormone homeostasis is critically important during stress *** rose(Rosa hybrida)flowers experience dehydration stress during postharvest handling,and jasmonic acid(JA)levels change as a result of this ***,how JA is involved in dehydration tolerance remains *** investigated the functions of the JA-and dehydration-induced RhHB1 gene,which encodes a homeodomain-leucine zipper Iγ-clade transcription factor,in rose *** RhHB1 decreased petal dehydration tolerance and resulted in a persistent increase in JA-Ile content and reduced dehydration *** elevated JA-Ile level had a detrimental effect on rose petal dehydration ***1 was shown to lower the transient induction of JA-Ile accumulation in response to *** addition to transcriptomic data,we obtained evidence that RhHB1 suppresses the expression of the lipoxygenase 4(RhlOX4)gene by directly binding to its promoter both in vivo and in *** propose that increased JA-Ile levels weaken the capacity for osmotic adjustment in petal cells,resulting in reduced dehydration *** conclusion,a JA feedback loop mediated by an RhHB1/RhlOX4 regulatory module provides dehydration tolerance by fine-tuning bioactive JA levels in dehydrated flowers.

关键词： flowers rose conclusion

Regulation of rose petal dehydration tolerance and senescence by RhNAP transcription factor via the modulation of cytokinin catabolism

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Molecular Horticulture 2021年第1期1卷 45-59页

作者： Jing Zou peitao lü liwei Jiang Kun liu Tao Zhang Jin Chen Yi Yao Yusen Cui Junping Gao Changqing Zhang Department of Ornamental Horticulture China Agricultural UniversityBeijing 100193China College of Horticulture FAFU-UCR Joint Center for Horticultural Biology and MetabolomicsHaixia Institute of Science and TechnologyFujian Agriculture and Forestry UniversityFuzhou 350002China

Petals and leaves share common evolutionary origins but have different phenotypic characteristics,such as the absence of stomata in the petals of most angiosperm *** NAC transcription factor,NAP,is involved in ABA responses and regulates senescence-associated genes,and especially those that affect stomatal ***,the regulatory mechanisms and significance of NAP action in senescing astomatous petals is unclear.A major limiting factor is failure of flower opening and accelerated *** goal is to understand the finely regulatory mechanism of dehydration tolerance and aging in rose *** functionally characterized RhNAP,an AtNAP-like transcription factor gene that is induced by dehydration and aging in astomatous rose ***(CKs)are known to delay petal senescence and we found that a cytokinin oxidase/dehydrogenase gene 6(RhCKX6)shares similar expression patterns with *** of RhNAP or RhCKX6 expression in rose petals by virus induced gene silencing markedly reduced petal dehydration tolerance and delayed petal *** CK levels in RhNAP-or RhCKX6-silenced petals were significantly higher than those of the ***,RhCKX6 expression was reduced in RhNAP-silenced *** suggests that the expression of RhCKX6 is regulated by *** one-hybrid experiments and electrophoresis mobility shift assays showed that RhNAP binds to the RhCKX6 promoter in heterologous in vivo system and in vitro,***,the expression of putative signal transduction and downstream genes of ABA-signaling pathways were also reduced due to the repression of PP2C homolog genes by RhNAP in rose *** together,our study indicates that the RhNAP/RhCKX6 interaction represents a regulatory step enhancing dehydration tolerance in young rose petals and accelerating senescence in mature petals in a stomata-independent manner.

关键词： Rosa hybrida RhNAP RhCKX6 Dehydration Petal senescence Cytokinins

The OsFTIP6-OsHB22-OsMYBR57 module regulates drought response in rice

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Molecular Plant 2022年第7期15卷 1227-1242页

作者： lijia Yang Ying Chen liang Xu Jiaxuan Wang Haoyue Qi Jiazhuo Guo liang Zhang Jun Shen Huanyu Wang Fan Zhang lijun Xie Wenjun Zhu peitao lü Qian Qian Hao Yu Shiyong Song State Key laboratory of Rice Biology Zhejiang Provincial Key Laboratory of Crop Genetic ResourcesInstitute of Crop ScienceCollege of Agriculture and BiotechnologyZhejiang UniversityHangzhou 310058China State Key laboratory of Rice Biology China National Rice Research InstituteHangzhou 311400China College of Horticulture FAFU-UCR Joint Center for Horticultural Biology and MetabolomicsHaixia Institute of Science and TechnologyFujian Agriculture and Forestry UniversityFuzhou 350002China Department of Biological Sciences and Temasek life Sciences laboratory National University of SingaporeSingapore 117543Singapore

Plants have evolved a sophisticated set of mechanisms to adapt to drought *** factors play crucial roles in plant responses to various environmental stimuli by modulating the expression of numerous stress-responsive ***,how the crosstalk between different transcription factor families orchestrates initiation of the key transcriptional network and the role of posttranscriptional modification of transcription factors,especially in cellular localization/trafficking in response to stress in rice,remain still largely *** this study,we isolated an Osmybr57 mutant that displays a drought-sensitive phenotype through a genetic screen for drought stress *** found that OsMYBR57,an MYB-related protein,directly regulates the expression of several key drought-related OsbZ/Ps in response to drought *** studies revealed that OsMYBR57 interacts with a homeodomain transcription factor,OsHB22,which also plays a positive role in drought *** further demonstrate that OsFTIP6 interacts with OsHB22 and promotes the nucleocytoplasmic translocation of OsHB22 into the nucleus,where OsHB22 cooperates with OsMYBR57 to regulate the expression of drought-responsive *** findings have revealed a mechanistic framework underlying the OsFTIP6-0sHB22-0sMYBR57 module-mediated regulation of drought response in *** OsFTIP6-mediated OsHB22 nucleocytoplasmic shuttling and OsMYBR57-0sHB22 regulation of OsbZIP transcription ensure precise control of expression of OslEA3 and Rab21,and thereby regulate the response to water deficiency in rice.

关键词： OsMYBR57 OsHB22 OsFTIP6 drought response nucleocytoplasmic trafficking rice

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香蕉MAP3K基因的克隆及表达

应用与环境生物学报 2022年第6期28卷 1520-1526页

作者：胡萌萌刘嘉鹏王斌黄玉吉吕培涛程春振福建农林大学园艺学院福州350002 山西农业大学园艺学院太谷030801

为研究一个受低温诱导的香蕉MAP3K基因的序列特征以及其与靶定它的miRNA(miR408d)和lncRNA(MSTRG.4851)的表达特征,以‘天宝蕉’为材料,采用反转录PCR(RT-PCR)技术克隆该基因,分析其核苷酸和编码蛋白序列特性,基于长链非编码RNA(lncRNA... 详细信息

为研究一个受低温诱导的香蕉MAP3K基因的序列特征以及其与靶定它的miRNA(miR408d)和lncRNA(MSTRG.4851)的表达特征,以‘天宝蕉’为材料,采用反转录PCR(RT-PCR)技术克隆该基因,分析其核苷酸和编码蛋白序列特性,基于长链非编码RNA(lncRNA)和小RNA(sRNA)测序数据分析MAP3K-miR408d-MSTRG.4851三者的表达趋势,并利用实时荧光定量PCR技术(qRT-PCR)验证它们在低温胁迫下的表达情况.结果显示:MAP3K编码序列(CDS)长为2 262 bp,可编码一个分子式为C3566H5625N1047O1090S32,氨基酸大小为753 aa,分子量为81 631.17,等电点为9.33的不稳定亲水性蛋白.MAP3K含有保守的PKinase结构域和MEKK结构域,不含跨膜结构和信号肽,与小果野蕉MAP3K亲缘关系最近.亚细胞定位预测结果显示MAP3K主要定位于外膜.蛋白互作预测结果显示MAP3K与MAPKK A亚族的3个MAP2K蛋白存在互作.MAP3K启动子含有大量的光响应和多种激素响应等元件.与组学测序结果一致,qRT-PCR结果显示MAP3K的表达受低温显著诱导,而靶定它的miRNA和lncRNA受低温抑制.本研究表明MAP3K的表达受miRNA和lncRNA调控,可能在香蕉响应低温胁迫过程中发挥重要作用.(图7表3参32)

关键词：香蕉丝裂原活化蛋白激酶激酶激酶基因克隆生物信息学分析表达模式

香蕉磷酸盐转运蛋白1(PHT1)基因家族的鉴定及表达

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应用与环境生物学报 2022年第6期28卷 1510-1519页

作者：麻荣慧田娜王斌刘嘉鹏胡萌萌刘范吕培涛程春振福建农林大学园艺学院福州350002 山西农业大学园艺学院太谷030801

磷酸盐转运蛋白1(phosphate transporter 1,PHT1)在植物对磷元素吸收过程中扮演着重要角色.为鉴定香蕉PHT1家族成员和分析它们的表达模式,对香蕉PHT1基因家族(MaPHT1)进行全基因组鉴定和生物信息学分析;并基于转录组数据研究MaPHT1s在... 详细信息

磷酸盐转运蛋白1(phosphate transporter 1,PHT1)在植物对磷元素吸收过程中扮演着重要角色.为鉴定香蕉PHT1家族成员和分析它们的表达模式,对香蕉PHT1基因家族(MaPHT1)进行全基因组鉴定和生物信息学分析;并基于转录组数据研究MaPHT1s在有和无印度梨形孢(Piriformospora indica)定殖的根系、45℃和4℃处理的叶片以及自然成熟和乙烯催熟的果实中的表达模式;同时利用实时荧光定量PCR(qRT-PCR)分析部分成员在不同磷浓度处理下有无P. indica定殖的香蕉根系中的表达情况.香蕉中共存在可分为4个亚家族(Group I-IV)的12个MaPHT1s,绝大多数成员含有1-2个外显子,其编码蛋白长度介于517-834 aa,具有10-12个跨膜结构域且均定位于质膜.MaPHT1s中存在1对串联重复基因和4对片段重复基因;启动子区含多种植物激素和逆境胁迫响应的顺式作用元件(cis-acting element)以及14种转录因子的结合位点(transcription factor binding sites,TFBS);MaPHT1s不同亚家族在香蕉不同组织部位表达差异较大,如Group III的MaPHT1-1、1-2和Group II的MaPHT1-7在根系、叶片和果实中均表达;Group IV中MaPHT1-8、1-9和1-11在根系与叶片中高表达.qRT-PCR结果显示:MaPHT1-5、1-7、1-8和1-9受低磷或缺磷诱导,接种P. indica诱导MaPHT1-1、1-5、1-7、1-8和1-9的表达.本研究表明MaPHT1s的表达受激素和逆境调控,不同亚家族成员的表达模式差异较大,推测部分成员在香蕉应答磷胁迫中发挥着重要作用.(图7表3参54)

关键词：香蕉高亲和力磷酸盐转运蛋白基因家族表达分析磷胁迫

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李早熟新品种早芙蓉的选育

果树学报 2022年第10期39卷 1961-1964页

作者：李建昆易小燕吕培涛罗水鑫宁火根林华忠方禄明陈发兴福建农林大学亚热带果树研究所福州350002 福建农林大学园艺学院福州350002 福建省连城县农业农村局福建连城366200 福建省清流县农业农村局福建清流365300 福建省将乐国有林场福建将乐353300

早芙蓉是从福建省连城县四堡镇上枧村的柰李选种圃中优选出来的李(Prunus salicina lindl.)新品种。果实近圆形,果顶平而微凹,梗洼深,缝合线明显、对称。平均单果质量66.55 g,果形指数为0.95,成熟期果皮鲜红色,色泽鲜亮,果粉厚,果肉深红... 详细信息

早芙蓉是从福建省连城县四堡镇上枧村的柰李选种圃中优选出来的李(Prunus salicina lindl.)新品种。果实近圆形,果顶平而微凹,梗洼深,缝合线明显、对称。平均单果质量66.55 g,果形指数为0.95,成熟期果皮鲜红色,色泽鲜亮,果粉厚,果肉深红色,肉质致密,纤维少,酸甜适口,可食率为96.1%,可溶性固形物含量(w)为12.97%,核小,果核质量1.53~1.85 g,黏核,卵圆形,表面粗糙,顶部钝圆。早芙蓉成熟期为6月底至7月初,比芙蓉李品种早15~20 d。适应性强,早果、丰产、稳产性突出,成年树每666.7 m^(2)产量可达2 541.9 kg(每666.7 m^(2)栽植37株)。

关键词：李新品种早芙蓉早熟

矮牵牛花瓣衰老和逆境胁迫响应相关NAC基因的鉴定与分析

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福建农业学报 2024年第6期39卷 700-710页

作者：杨应杰张付昆穆静怡付鲁峰陈倬李华关夏玉吕培涛福建农林大学园艺学院福建福州350002

【目的】NAC(NAM, ATAF and CUC)参与植物生长发育和多种逆境胁迫响应过程的调控。本文旨在鉴定和研究对矮牵牛生长发育和逆境胁迫响应的关键NAC成员,为优质抗逆矮牵牛育种提供基因资源。【方法】以腋生矮牵牛(Petunia axillaris)基因... 详细信息

【目的】NAC(NAM, ATAF and CUC)参与植物生长发育和多种逆境胁迫响应过程的调控。本文旨在鉴定和研究对矮牵牛生长发育和逆境胁迫响应的关键NAC成员,为优质抗逆矮牵牛育种提供基因资源。【方法】以腋生矮牵牛(Petunia axillaris)基因组为参考基因组,利用矮牵牛花器官衰老过程、烟草脆裂病毒(Tobacco rattle virus,TRV)侵染、低磷、低温、NaCl、铜离子和干旱胁迫处理后的转录组数据,分析矮牵牛NAC基因(PaNACs)差异表达情况,并对差异表达PaNACs的启动子顺式作用元件及转录因子结合位点进行分析。利用实时荧光定量PCR验证了部分差异表达PaNACs在矮牵牛花衰老过程中的表达情况,并预测了差异表达PaNACs编码蛋白的潜在靶基因。【结果】鉴定的131个PaNAC基因中,59个(45.04%)被鉴定为花器官衰老和逆境胁迫响应过程中的差异表达基因。PaNAC72、 PaNAC22、 PaNAC29、 PaNAC40、 PaNAC2、 PaNAC90、 PaNAC83、 PaNAC56、 PaNAC36和PaNAC35在至少3个生物学过程响应中差异表达显著,其中拟南芥衰老关键基因AtNAP的直系同源基因PaNAC29在花器官衰老过程和低温、低磷、铜离子胁迫逆境处理中显著上调表达;PaNAC72在除受铜离子胁迫外的所有6种处理中表达差异显著;PaNAC22在花器官衰老过程和低温和低磷胁迫中上调表达,在铜离子和干旱逆境下调表达。启动子分析结果显示这10个PaNAC启动子区域存在多种逆境胁迫响应相关元件,且大量响应衰老和逆境胁迫的差异表达基因的启动子区域存在NAC的结合位点。【结论】PaNACs广泛参与矮牵牛生长发育及逆境胁迫响应,其中PaNAC29可能是花衰老关键的正调控因子,PaNAC72广泛响应多种逆境胁迫。

关键词：矮牵牛 NAC 转录组衰老胁迫生物信息学

Tsunami hazard and mitigation analysis for bathing beaches in China based on numerical simulations

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Acta Oceanologica Sinica 2022年第12期41卷 27-37页

作者： Jingming Hou Wei lu Tingting Fan peitao Wang National Marine Environmental Forecasting Center Beijing 100081China Key laboratory of Marine Hazards Forecasting Ministry of Natural ResourcesBeijing 100081China

Bathing beaches are usually the first to suffer disasters when tsunamis occur,owing to their proximity to the *** large seismic fault zones are located off the coast of *** impact of each tsunami scenario on Chinese bathing beaches is *** this study,numerical models of the worst tsunami scenarios associated with seismic fault zones were considered to assess the tsunami hazard of bathing beaches in *** results show that tsunami waves from the Pacific Ocean could affect the East China Sea coast through gaps between the Ryukyu *** Zhejiang and Shanghai coasts would be threatened by a tsunami from Ryukyu Trench,and the coasts of Hainan and Guangdong provinces would be threatened by a tsunami from the Manila *** tsunami hazard associated with the Philippine Trench scenario needs particular *** to China’s offshore topography,the sequential order of tsunami arrival times to coastal provinces in several tsunami scenarios is almost the *** to the tsunami hazard analysis results,Yalongwan Beach and eight other bathing beaches are at the highest hazard level.A high-resolution numerical calculation model was established to analyze the tsunami physical characteristics for the high-risk bathing *** explore mitigating effects of a tsunami disaster,this study simulated tsunami propagation with the addition of *** experimental results show that the tsunami prevention seawalls constructed in an appropriate shallow water location have some effect on reducing tsunami *** separated by a certain distance work even *** analysis results can provide a scientific reference for subsequent preventive measures such as facility construction and evacuation.

关键词： tsunami bathing beach hazard mitigation

Thermal transport and phase transitions of zirconia by on-the-fly machine-learned interatomic potentials

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npj Computational Materials 2021年第1期7卷 1426-1434页

作者： Carla Verdi Ferenc Karsai peitao liu Ryosuke Jinnouchi Georg Kresse University of Vienna Faculty of PhysicsComputational Materials PhysicsKolingasse 14-161090 ViennaAustria VASP Software GmbH Sensengasse 81090 ViennaAustria Toyota Central R&D labs. Inc.Aichi 480-1192Japan

Machine-learned interatomic potentials enable realistic finite temperature calculations of complex materials properties with firstprinciples *** is not yet clear,however,how accurately they describe anharmonic properties,which are crucial for predicting the lattice thermal conductivity and phase transitions in solids and,thus,shape their technological *** we employ a recently developed on-the-fly learning technique based on molecular dynamics and Bayesian inference in order to generate an interatomic potential capable to describe the thermodynamic properties of zirconia,an important transition metal *** machine-learned potential accurately captures the temperature-induced phase transitions below the melting *** further showcase the predictive power of the potential by calculating the heat transport on the basis of Green–Kubo theory,which allows to account for anharmonic effects to all *** study indicates that machine-learned potentials trained on the fly offer a routine solution for accurate and efficient simulations of the thermodynamic properties of a vast class of anharmonic materials.

关键词： materials transitions harmonic

First-principles study on the electronic structure of Pb_(10−x)Cu_(x)(PO_(4))_(6)O(x=0,1)

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Journal of Materials Science & Technology 2024年第4期171卷 66-70页

作者： Junwen lai Jiangxu li peitao liu Yan Sun Xing-Qiu Chen Shenyang National laboratory for Materials Science Institute of Metal ResearchChinese Academy of SciencesShenyang 110016China School of Materials Science and Engineering University of Science and Technology of ChinaShenyang 110016China

Recently,lee et *** the experimental discovery of room-temperature ambient-pressure super-conductivity in a Cu-doped lead-apatite(lK-99)(arXiv:2307.12008,arXiv:2307.12037).Remarkably,the claimed superconductivity can persist up to 400 K at ambient *** the experimental im-plication,the electronic structure of lK-99 has not yet been ***,we investigate the electronic structures of lK-99 and its parent compound using first-principles calculations,aiming to elucidate the doping effects of *** results reveal that the parent compound Pb_(10)(PO_(4))_(6)O is an insulator,while Cu doping induces an insulator-metal transition and thus volume *** band structures of lK-99 around the Fermi level are featured by a half-filled flat band and a fully-occupied flat *** two very flat bands arise from both the 2p orbitals of 1/4-occupied O atoms and the hybridization of the 3d orbitals of Cu with the 2p orbitals of its nearest-neighboring O ***,we observe four van Hove singularities on these two flat ***,we show that the flat band structures can be tuned by including electronic correlation effects or by doping different *** find that among the considered doping elements(Ni,Cu,Zn,Ag,and Au),both Ni and Zn doping result in the gap opening,whereas Au exhibits doping effects more similar to Cu than *** work establishes a foundation for fu-ture studies to investigate the role of unique electronic structures of lK-99 in its claimed superconducting properties.

关键词： First-principles calculations Density functional theory Electronic structur e Superconductivity Flat bands Strongly correlated electrons