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Integrated metabolo-transcriptomics and functional characterization reveals that the wheat auxin receptor TIR1 negatively regulates defense against Fusarium graminearum

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Journal of Integrative Plant Biology 2021年第2期63卷 340-352页

作者： peisen su Lanfei Zhao Wen Li Jinxiao Zhao Jun Yan Xin Ma Anfei Li Hongwei Wang Lingrang Kong State Key Laboratory of Crop Biology Shandong Key Laboratory of Crop BiologyCollege of AgronomyShandong Agricultural UniversityTai’an 271018China College of Information Science and Engineering Shandong Agricultural UniversityTai'an 271018China

Fusarium head blight(FHB) caused by Fusarium graminearum Schwabe(teleomorph Gibberella zeae(Schw.) Perch) results in large yield losses in annual global wheat production. Although studies have identified a number of wheat FHB resistance genes,a deeper understanding of the mechanisms underlying host plant resistance to F. graminearum is required for the control of FHB. Here, an integrated metabolomics and transcriptomics analysis of infected wheat plants(Triticum aestivum L.) enabled identification of 789 differentially accumulated metabolites, including flavonoids, phenolamides, tryptamine derivatives, and phytohormones, and revealed altered expression of more than 100 genes that function in the biosynthesis or regulation of these pathways. Our data regarding the effects of F. graminearum infection on flavonoids and auxin signaling led to follow-up experiments that showed that exogenous kaempferide and apigenin application on spikes increased wheat resistance to FHB,while exogenous auxin treatment increased FHB susceptibility. RNAi-mediated knockdown of the gene encoding the auxin receptor, Ta TIR1, increased FHB resistance. Our data supported the use of Ta TIR1 knockdown in controlling FHB. Our study provides insights on the wheat response to *** infection and its FHB resistance mechanisms while illustrating the potential of Ta TIR1 knockdown in increasing FHB resistance during crop improvement programs.

关键词： FHB metabolomics TaTIR1 transcriptomics

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大田玉米接种禾谷镰刀菌的不同方法比较

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中国种业 2024年第2期 88-92页

作者：刘建陈旭李新新邹文玉唐国来张东鹤苏培森徐瑞斌叶海龙上海市农业技术推广服务中心上海201103 上海航育种子有限公司上海201100 聊城大学农学与农业工程学院山东聊城252000 北京市农林科学院玉米研究所北京100097

禾谷镰刀菌是危害玉米田间种植最严重的真菌病害之一,研究玉米禾谷镰刀菌的接种规律对于了解玉米禾谷镰刀菌病害的发生机制及培育和鉴定抗禾谷镰刀菌玉米品种资源等方面具有重要意义。采用灌根法、注射法和喷雾法对苗期、拔节期和抽穗... 详细信息

禾谷镰刀菌是危害玉米田间种植最严重的真菌病害之一,研究玉米禾谷镰刀菌的接种规律对于了解玉米禾谷镰刀菌病害的发生机制及培育和鉴定抗禾谷镰刀菌玉米品种资源等方面具有重要意义。采用灌根法、注射法和喷雾法对苗期、拔节期和抽穗期玉米进行禾谷镰刀菌田间接种,结果表明在玉米抽穗期采用注射法进行接种效果显著。

关键词：玉米禾谷镰刀菌接种方式灌根法注射法喷雾法

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Genome-wide identification,classification,evolutionary analysis and gene expression patterns of the protein kinase gene family in wheat and Aegilops tauschii

Genome-wide identification,classification,evolutionary analy...

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第八届全国小麦基因组学及分子育种大会

作者： Jun Yan peisen su Zhaoran Wei Eviatar Nevo Lingrang Kong College of Information Science and Engineering Shandong Agricultural University State Key Laboratory of Crop Biology Shandong Key Laboratory of Crop BiologyCollege of AgronomyShandong Agricultural University Institute of Evolution University of Haifa

The protein kinase(PK) gene superfamily is one of the largest families in plants and participates in various plant processes,including growth,development,and stress *** better understand wheat PKs,we conducted genome-wide identification,classification,evolutionary analysis and expression profiles of wheat and Aegilops tauschii *** identified 3269,1213 and 1448 typical PK genes vaTriticum aestivum,Triticum urartu and Aegilops tauschii,respectively,and classified them into major groups and *** distributions and gene structures were analyzed and *** conserved intron-exon structures within the conserved kinase domain were found in ***,*** and ***,as well as the primitive land plants Selaginella moellendorffii and Physcomitrella patens,imp lying the important roles and conserved evolutionary history of these *** analyized the collinearity of *** PKs and identified PKs from polyploidizations and tandem duplication *** expression pattern analysis of *** PKs revealed tissue-specific and stress-specific expression profiles,hinting that some wheat PKs may regulate abiotic and biotic stress response signaling ***-PCR of 15 selected PKs were performed under drought treatment and with infection of Fusarium graminearum to validate the prediction of *** results will provide the foundational information for further studies on the molecular functions of wheat PKs.

关键词： Wheat protein kinase family conserved exon-intron structures Collinearity events Expression pattern

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桩筏复合地基在盾构下穿时的力学特性研究

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现代隧道技术 2020年第S1期57卷 502-506页

作者：苏培森中铁第四勘察设计院集团有限公司武汉430063

桩筏复合地基常用于地质较软弱的工程中,桩筏结构能够有效提高地基的承载能力和抗变形能力,桩-筏板-地基三者通过协调变形来承担上部荷载作用。盾构隧道下穿桩筏结构时,隧道的开挖支护将改变桩-筏复合地基的边界条件并产生应力扰动,从... 详细信息

桩筏复合地基常用于地质较软弱的工程中,桩筏结构能够有效提高地基的承载能力和抗变形能力,桩-筏板-地基三者通过协调变形来承担上部荷载作用。盾构隧道下穿桩筏结构时,隧道的开挖支护将改变桩-筏复合地基的边界条件并产生应力扰动,从而对上覆建(构)筑物的安全造成威胁。通过采用有限元软件对盾构隧道下穿既有桩筏复合地基进行平面应变分析,探究了隧道开挖对桩筏结构内力和地基应力影响的变化规律,得出如下结论:(1)桩筏复合地基结构的筏板和边跨处桩基的弯矩值较大,跨中处桩基的轴力较大;(2)盾构隧道的施工对刚度较大的桩筏结构的内力影响较小,隧道开挖对桩筏结构的内力附加作用不显著;(3)桩筏结构在岩土介质中具有隔离作用,可以有效减小隧道开挖对上覆建构筑物的影响。

关键词：高速铁路力学特性数值计算桩筏复合地基盾构隧道

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温州深厚软土地层盾构隧道建设的地质风险及控制研究

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城市轨道交通研究 2023年第12期26卷 200-203页

作者：苏培森中铁第四勘察设计院集团有限公司武汉430063

目的:温州地处东海之滨,根据温州市轨道交通既有勘察资料显示,约60%区间隧道洞身处于深厚淤泥及淤泥质软土地层中。而当前对深厚软土地层盾构隧道建设经验不足,需开展软土地层盾构隧道建设的地质风险分析并提出控制措施。方法:通过查阅... 详细信息

目的:温州地处东海之滨,根据温州市轨道交通既有勘察资料显示,约60%区间隧道洞身处于深厚淤泥及淤泥质软土地层中。而当前对深厚软土地层盾构隧道建设经验不足,需开展软土地层盾构隧道建设的地质风险分析并提出控制措施。方法:通过查阅和分析既有研究成果,探讨了软土地层盾构隧道建设的研究现状,在此基础上结合温州软土既有工程勘察实践,总结了其工程力学特性,借助有限元软件分析了隧道衬砌结构的受力特点,并基于软土盾构隧道病害的不利因素分析,进而提出风险控制措施。结果及结论:从温州深厚软土的工程力学特性和隧道衬砌结构受力特点出发,分析总结了深厚软土地层盾构隧道建设的地质风险,提出了以扰动控制为核心的相关控制措施,以供温州轨道交通及其他深厚软土地层盾构隧道建设。

关键词：软土地层盾构隧道地质风险风险控制

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Genome-wide and evolutionary analysis of the class Ⅲ peroxidase gene family in wheat and Aegilops tauschii reveals that some members are involved in stress responses

Genome-wide and evolutionary analysis of the class Ⅲ peroxi...

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第十届全国小麦基因组学及分子育种大会

作者： Jun Yan peisen su Wen Li Yan Zhao Xin Ma Hongwei Wang Eviatar Nevo Lingrang Kong College of Information Science and Engineering Shandong Agricultural University State Key Laboratory of Crop Biology Shandong Key Laboratory of Crop BiologyCollege of AgronomyShandong Agricultural University Institute of Evolution University of Haifa

The class Ⅲ peroxidase(PRX) gene family is a plant-specific member of the PRX superfamily that is closely related to various physiological processes,such as cell wall loosening,lignification,and abiotic and biotic stress ***,its classification,evolutionary history and gene expression patterns are unclear in wheat and Aegilops ***,we identified 374,159 and 169 PRXs in Triticum aestivum,Triticum urartu and ***,*** with PRXs detected from eight otherplants,they were classified into 18 *** subfamilies V to XVIII,a conserved exon-intron structure within the "001" exon phases was detected in the PRX *** on the analysis,we proposed a phylogenetic model to infer the evolutionary history of the exon-intron structures of PRX subfamilies.A comparative genomics analysis showed that subfamily VII could be the ancient subfamily that originated from green algae(Chlamydomonas reinhardtii).Further integrated analysis of chromosome locations and collinearity events of PRX genes suggested that both whole genome duplication(WGD) and tandem duplication(TD)events contributed to the expansion of *** PRXs(TaePRXs) during wheat *** validate functions of these genes in the regulation of various physiological processes,the expression patterns of PRXs in different tissues and under various stresses were studied using public microarray *** results suggested that there were distinct expression patterns among different tissues and PRXs could be involved in biotic and abiotic responses in ***-PCR was performed on samples exposed to drought,phytohormone treatments and Fusarium graminearum infection to validate the microarray *** predicted subcellular localizations of some TaePRXs were consistent with the confocal microscopy *** predicted that some TaePRXs had hormone-responsive cis-elements in their promoter regions and validated these predicted cis-acting elements by sequencin

关键词： Wheat class Ⅲ peroxidase gene family Conserved exon-intron structures Collinearity events Expression pattern

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A wheat Peroxidase Gene TaPRX mediates the defence responses to drought and salinity stresses in transgenic wheat

A wheat Peroxidase Gene TaPRX mediates the defence responses...

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第十届全国小麦基因组学及分子育种大会

作者： peisen su Jun Yan Liang Wang Wen Li Jinxiao Zhao Xin Ma Anfei Li Hongwei Wang Lingrang Kong State Key Laboratory of Crop Biology Shandong Agricultural University College of Information Science and Engineering Shandong Agricultural University

Peroxidases(PRX),a large group of widely distributed enzymes,play a central role in regulation of many plant processes,including abiotic ***,far less information of molecular mechanisms is available on abiotic-responsive PRX genes in ***,we reported insights into the function of a wheat(Triticum aestivum) class III peroxidases gene *** to the identification PRXs of twelve plants,we proposed a evolutionary model of this TaPRX *** inferred that this TaPRX might experience some exon fusion events in *** expression pattern of this TaPRX was performed in different tissues by qRT-PCR,and the result showed that it exhibited relatively higher expression levels in root than stem and *** expression pattern of abiotic stresses showed that it was up-regulated by the treatment with PEG6000,NaCl,H202,and hormones(including SA,JA,ABA).We constructed the TaPRX-overexpressing transgenic wheat,and the result exhibited that it significantly enhanced tolerances to drought and salinity stresses in transgenic wheat than *** drought and salinity stresses,the transgenic wheat plants had a higher survive rate,higher relative water content,and higher shoot length than WT,but the root length was almost no *** results indicated that transgenic plants enhanced drought and salinity tolerance by enhancing oxidative stress tolerance,such as lower levels of reactive oxygen species(ROS) accumulation,MDA content and higher antioxidant enzymes activities of SOD,CAT,***,a subset of stress-related genes were up-regulated by overexpression of the *** together,these results demonstrate that TaPRX functions act as a positive factor under drought and salinity stresses by controlling ROS homeostasis and regulating transcription of stress related genes.

关键词： Wheat Drought and salinity stresses Peroxidases TaPRX

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Application of Brachypodium genotypes in analysis of Type Ⅱ resistance to Fusarium head blight(FHB)

Application of Brachypodium genotypes in analysis of Type Ⅱ...

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第八届全国小麦基因组学及分子育种大会

作者： peisen su Xiuxiu Guo Liang Wang Guanghui Yu Biao Wang Lanfei Zhao Xin Ma Anfei Li Jiajie Wu Hongwei Wang Lingrang Kong State Key Laboratory of Crop Biology Shan dong Key Laboratory of Crop BiologyCollege of AgronomyShandong Agricultural University

The host resistance mechanisms to Fusarium head blight(FHB) are still largely *** distachyon 21(Bd21) is able interact with *** while its application in evaluation of genes conferring the major FHB resistance(type II) is still to be *** this study,we found the spores mainly germinate on pistil,then the hyphae quickly extend to the bottom of the floret and entered into spike *** carefully examined the disease development process on spikes of *** in comparison with that in wheat and barley,and the expansion rates over the rachis node of inoculated spike let was proved to be an important index to evaluate type II resistance of *** under a controlled condition at 28°C,50%-70%*** using this strategy,we cloned a TaTGA2 gene in wheat and proved its positive reguktbn in FHB resistance,by using BMSV-induced gene silencing(VIGS) and tilling mutants of tetraploid *** examination expression of TaTGA2 and its downstream genes indicated that TaTGA2 probably regulated FHB resistance by depressing the SA signaling ***,a total of 49 barchypodium germplasms were further screened for FHB resistance,among which 10 were found to exhibit more susceptibility than Bd21,with transformation efficiency from 6.38%to 24.44%.These germplasms might be alternative hosts expressing genes positively regulating FHB *** this study,no consistence was found for the FHB resistance between coleoptiles and spikes neither in transgenic plants and natural germplasms.

关键词： Brachypodium distachyon Fusanum head blight(FHB) Rachis TaTGA2

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小麦赤霉病抗病机制研究进展

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生物技术进展 2021年第5期11卷 599-609页

作者：苏培森聊城大学农学院山东聊城252000

小麦赤霉病是一种小麦穗部病害,严重影响小麦的产量和品质。挖掘小麦赤霉病抗性基因,揭示其抗病机制,对于提高小麦赤霉病抗性,推动小麦赤霉病抗性育种进程具有重要的意义。系统阐述了抗赤霉病相关QTL、多组学研究、细胞壁防卫、信号转... 详细信息

小麦赤霉病是一种小麦穗部病害,严重影响小麦的产量和品质。挖掘小麦赤霉病抗性基因,揭示其抗病机制,对于提高小麦赤霉病抗性,推动小麦赤霉病抗性育种进程具有重要的意义。系统阐述了抗赤霉病相关QTL、多组学研究、细胞壁防卫、信号转导、次生代谢物合成、识别应答等小麦赤霉病抗性机制的研究进展,并对未来小麦赤霉病抗性机制的研究方向进行了探讨。希望以此加深研究者对小麦赤霉病抗性机制的了解,为未来小麦抗赤霉病分子机制研究提供理论基础,为小麦抗赤霉病遗传改良提供丰富的基因资源。

关键词：小麦赤霉病抗性机制

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小麦-禾谷镰孢菌互作机制的研究进展

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中国科学：生命科学 2021年第11期51卷 1493-1507页

作者：苏培森葛文扬王宏伟孔令让山东农业大学农学院/作物生物学国家重点实验室泰安271018 聊城大学农学院聊城252000

禾谷镰孢菌(俗称禾谷镰刀菌,Fusarium graminearum)引起的小麦赤霉病是小麦生产上一种极具毁灭性的真菌病害,在世界各主要产麦国普遍发生,我国是世界上受害面积最大的国家,该病害严重威胁我国粮食生产和食品安全.解析小麦-禾谷镰孢菌之... 详细信息

禾谷镰孢菌(俗称禾谷镰刀菌,Fusarium graminearum)引起的小麦赤霉病是小麦生产上一种极具毁灭性的真菌病害,在世界各主要产麦国普遍发生,我国是世界上受害面积最大的国家,该病害严重威胁我国粮食生产和食品安全.解析小麦-禾谷镰孢菌之间的互作机制,挖掘有效抗病基因,对于解决小麦赤霉病这一世界难题具有重要意义.本文对禾谷镰孢菌致病机制,小麦与禾谷镰孢菌致病因子DON毒素互作机制,禾谷镰孢菌侵染与小麦识别互作机制以及寄主-病原菌基因水平转移等研究进展进行了综述.

关键词：小麦赤霉病禾谷镰孢菌小麦-禾谷镰孢菌互作呕吐毒素(DON)

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